PLEKHA1 ORF clone FHC09757

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC09757

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Clone info.

Species

human

Clone Name

pFN21AE0696 Sequence (with vector) Restriction Map

Product ID

FHC09757

Flexi Clone PID

FXC09757

ORF Sequence

DNA sequence (1212 bp)
Protein sequence (404 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528495]

Gene

KIEE0696

Symbol

PLEKHA1

Alias : TAPP1

Description

pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1, transcript variant 1

Original Clone ID

cs02196

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1212 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 404 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7629.1 1.7e-145 100.0 PLEKHA1 CCDS55730.1 1.8e-107 92.4 PLEKHA1 CCDS81517.1 2.3e-107 100.0 PLEKHA1 CCDS75732.1 1.6e-43 47.0 PLEKHA2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001849 11 112 PF00169 Pleckstrin homology domain IPR001849 192 285 PF00169 Pleckstrin homology domain HMMSmart IPR001849 8 114 SM00233 Pleckstrin homology domain IPR001849 192 291 SM00233 Pleckstrin homology domain ProfileScan IPR001849 7 112 PS50003 Pleckstrin homology domain IPR001849 191 289 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016871969 404 603075,607772 100.0 100.0 XP_005270073 404 603075,607772 100.0 100.0 NP_067635 404 603075,607772 100.0 100.0 XP_016871970 404 603075,607772 100.0 100.0 XP_016871971 404 603075,607772 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0696 Download

>KIEE0696  1212 bp
ATGCCTTATGTGGATCGTCAGAATCGCATTTGTGGTTTTCTAGACATTGAAGAAAATGAA
AACAGTGGGAAATTTCTTCGAAGGTACTTCATACTGGATACCAGAGAAGATAGTTTCGTG
TGGTACATGGATAATCCACAGAACCTACCTTCTGGATCATCACGTGTTGGAGCCATTAAG
CTTACCTACATTTCAAAGGTTAGCGATGCTACTAAGCTAAGGCCAAAGGCGGAGTTCTGT
TTTGTTATGAATGCAGGAATGAGGAAGTACTTCCTACAAGCCAATGATCAGCAGGACCTA
GTGGAATGGGTAAATGTGTTAAACAAAGCTATAAAAATTACAGTACCAAAGCAGTCAGAC
TCACAGCCTAATTCTGATAACCTAAGTCGCCATGGTGAATGTGGGAAAAAGCAAGTGTCT
TACAGAACTGATATTGTTGGTGGCGTACCCATCATTACTCCCACTCAGAAAGAAGAAGTA
AATGAATGTGGTGAAAGTATTGACAGAAATAATCTGAAACGGTCACAAAGCCATCTTCCT
TACTTTACTCCTAAACCACCTCAAGATAGTGCGGTTATCAAAGCTGGATATTGTGTAAAA
CAAGGAGCAGTGATGAAAAACTGGAAGAGAAGATATTTTCAATTGGATGAAAACACAATA
GGCTACTTCAAATCTGAACTGGAAAAGGAACCTCTTCGCGTAATACCACTTAAAGAGGTT
CATAAAGTCCAGGAATGTAAGCAAAGCGACATAATGATGAGGGACAACCTCTTTGAAATT
GTAACAACGTCTCGAACTTTCTATGTGCAGGCTGATAGCCCTGAAGAGATGCACAGTTGG
ATTAAAGCAGTCTCTGGCGCCATTGTAGCACAGCGGGGTCCCGGCAGATCTGCGTCTTCT
GAGCATCCCCCCGGTCCTTCAGAATCCAAACACGCTTTCCGTCCTACCAACGCAGCCACC
GCCACCTCACATTCCACAGCCTCTCGCAGCAACTCTTTGGTCTCAACCTTTACCATGGAG
AAGCGAGGATTTTACGAGTCTCTTGCCAAGGTCAAGCCAGGGAACTTCAAGGTCCAGACT
GTCTCTCCAAGAGAACCAGCTTCCAAAGTGACTGAACAAGCTCTGTTAAGACCTCAAAGT
AAAAATGGCCCTCAGGAAAAAGATTGTGACCTAGTAGACTTGGACGATGCGAGCCTTCCG
GTCAGTGACGTG

ORF Protein

Cloned ORF protein sequence for pFN21AE0696 Download

>KIEE0696  404 aa
MPYVDRQNRICGFLDIEENENSGKFLRRYFILDTREDSFVWYMDNPQNLPSGSSRVGAIK
LTYISKVSDATKLRPKAEFCFVMNAGMRKYFLQANDQQDLVEWVNVLNKAIKITVPKQSD
SQPNSDNLSRHGECGKKQVSYRTDIVGGVPIITPTQKEEVNECGESIDRNNLKRSQSHLP
YFTPKPPQDSAVIKAGYCVKQGAVMKNWKRRYFQLDENTIGYFKSELEKEPLRVIPLKEV
HKVQECKQSDIMMRDNLFEIVTTSRTFYVQADSPEEMHSWIKAVSGAIVAQRGPGRSASS
EHPPGPSESKHAFRPTNAATATSHSTASRSNSLVSTFTMEKRGFYESLAKVKPGNFKVQT
VSPREPASKVTEQALLRPQSKNGPQEKDCDLVDLDDASLPVSDV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0696 Download

>pFN21AE0696  5917 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCTTATGTGGATCGTCAGAATCGCATTTGTGG
TTTTCTAGACATTGAAGAAAATGAAAACAGTGGGAAATTTCTTCGAAGGTACTTCATACT
GGATACCAGAGAAGATAGTTTCGTGTGGTACATGGATAATCCACAGAACCTACCTTCTGG
ATCATCACGTGTTGGAGCCATTAAGCTTACCTACATTTCAAAGGTTAGCGATGCTACTAA
GCTAAGGCCAAAGGCGGAGTTCTGTTTTGTTATGAATGCAGGAATGAGGAAGTACTTCCT
ACAAGCCAATGATCAGCAGGACCTAGTGGAATGGGTAAATGTGTTAAACAAAGCTATAAA
AATTACAGTACCAAAGCAGTCAGACTCACAGCCTAATTCTGATAACCTAAGTCGCCATGG
TGAATGTGGGAAAAAGCAAGTGTCTTACAGAACTGATATTGTTGGTGGCGTACCCATCAT
TACTCCCACTCAGAAAGAAGAAGTAAATGAATGTGGTGAAAGTATTGACAGAAATAATCT
GAAACGGTCACAAAGCCATCTTCCTTACTTTACTCCTAAACCACCTCAAGATAGTGCGGT
TATCAAAGCTGGATATTGTGTAAAACAAGGAGCAGTGATGAAAAACTGGAAGAGAAGATA
TTTTCAATTGGATGAAAACACAATAGGCTACTTCAAATCTGAACTGGAAAAGGAACCTCT
TCGCGTAATACCACTTAAAGAGGTTCATAAAGTCCAGGAATGTAAGCAAAGCGACATAAT
GATGAGGGACAACCTCTTTGAAATTGTAACAACGTCTCGAACTTTCTATGTGCAGGCTGA
TAGCCCTGAAGAGATGCACAGTTGGATTAAAGCAGTCTCTGGCGCCATTGTAGCACAGCG
GGGTCCCGGCAGATCTGCGTCTTCTGAGCATCCCCCCGGTCCTTCAGAATCCAAACACGC
TTTCCGTCCTACCAACGCAGCCACCGCCACCTCACATTCCACAGCCTCTCGCAGCAACTC
TTTGGTCTCAACCTTTACCATGGAGAAGCGAGGATTTTACGAGTCTCTTGCCAAGGTCAA
GCCAGGGAACTTCAAGGTCCAGACTGTCTCTCCAAGAGAACCAGCTTCCAAAGTGACTGA
ACAAGCTCTGTTAAGACCTCAAAGTAAAAATGGCCCTCAGGAAAAAGATTGTGACCTAGT
AGACTTGGACGATGCGAGCCTTCCGGTCAGTGACGTGGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT