BDKRB1 ORF clone FHC09533

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Halotag ORF Clone FHC09533

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Clone info.

Species

human

Clone Name

pFN21AE9529 Sequence (with vector) Restriction Map

Product ID

FHC09533

ORF Sequence

DNA sequence (1059 bp)
Protein sequence (353 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9529

Symbol

BDKRB1

Alias : B1BKR, B1R, BKB1R, BKR1, BRADYB1

Description

bradykinin receptor B1

Original Clone ID

cp99529

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1059 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 353 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9943.1 2.5e-132 100.0 BDKRB1 CCDS9942.1 2.4e-38 37.7 BDKRB2 CCDS3137.1 1e-31 32.8 AGTR1 CCDS82855.1 1.1e-31 32.8 AGTR1 CCDS82854.1 1.1e-31 32.8 AGTR1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition BlastProDom NULL 3 48 PD012188 NULL FPrintScan IPR001186 8 22 PR00993 B1 bradykinin receptor IPR001186 22 38 PR00993 B1 bradykinin receptor IPR000276 39 63 PR00237 GPCR IPR000276 72 93 PR00237 GPCR IPR000496 92 103 PR00425 Bradykinin receptor IPR000496 112 125 PR00425 Bradykinin receptor IPR000276 117 139 PR00237 GPCR IPR001186 122 133 PR00993 B1 bradykinin receptor IPR000276 153 174 PR00237 GPCR IPR000496 166 179 PR00425 Bradykinin receptor IPR001186 170 185 PR00993 B1 bradykinin receptor IPR000496 185 198 PR00425 Bradykinin receptor IPR001186 195 206 PR00993 B1 bradykinin receptor IPR000276 203 226 PR00237 GPCR IPR000496 218 230 PR00425 Bradykinin receptor IPR001186 219 234 PR00993 B1 bradykinin receptor IPR001186 239 256 PR00993 B1 bradykinin receptor IPR000276 248 272 PR00237 GPCR IPR001186 267 286 PR00993 B1 bradykinin receptor IPR000276 294 320 PR00237 GPCR IPR000496 313 325 PR00425 Bradykinin receptor HMMPfam IPR000276 54 312 PF00001 GPCR ProfileScan IPR017452 54 312 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 38 RVLPTFIISICFFGLLGNLFVLL 60 PRIMARY 23 2 72 AEIYLANLAASDLVFVLGLPFWA 94 SECONDARY 23 3 107 ALLCRVINGVIKANLFISIFLVV 129 PRIMARY 23 4 156 VTCVLIWVVGGLLSIPTFLLRSI 178 PRIMARY 23 5 184 LNITACILLLPHEAWHFARIVEL 206 SECONDARY 23 6 209 LGFLLPLAAIVFFNYHILASLR 230 PRIMARY 22 7 252 ILTLVVAFLVCWAPYHFFAFLEF 274 PRIMARY 23 8 293 GLQLANFFAFTNSSLNPVIYVFV 315 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_000701 353 600337 100.0 100.0 NP_000614 391 113503 37.7 91.8 NP_000676 359 106165,145500,267430 32.8 87.3 NP_033611 359 106165,145500,267430 32.8 87.3 NP_114438 388 106165,145500,267430 32.8 87.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9529 Download

>KIEE9529  1059 bp
ATGGCATCATCCTGGCCCCCTCTAGAGCTCCAATCCTCCAACCAGAGCCAGCTCTTCCCT
CAAAATGCTACGGCCTGTGACAATGCTCCAGAAGCCTGGGACCTGCTGCACAGAGTGCTG
CCGACATTTATCATCTCCATCTGTTTCTTCGGCCTCCTAGGGAACCTTTTTGTCCTGTTG
GTCTTCCTCCTGCCCCGGCGGCAACTGAACGTGGCAGAAATCTACCTGGCCAACCTGGCA
GCCTCTGATCTGGTGTTTGTCTTGGGCTTGCCCTTCTGGGCAGAGAATATCTGGAACCAG
TTTAACTGGCCTTTCGGAGCCCTCCTCTGCCGTGTCATCAACGGGGTCATCAAGGCCAAT
TTGTTCATCAGCATCTTCCTGGTGGTGGCCATCAGCCAGGACCGCTACCGCGTGCTGGTG
CACCCTATGGCCAGCCGGAGGCAGCAGCGGCGGAGGCAGGCCCGGGTCACCTGCGTGCTC
ATCTGGGTTGTGGGGGGCCTCTTGAGCATCCCCACATTCCTGCTGCGATCCATCCAAGCC
GTCCCAGATCTGAACATCACCGCCTGCATCCTGCTCCTCCCCCATGAGGCCTGGCACTTT
GCAAGGATTGTGGAGTTAAATATTCTGGGTTTCCTCCTACCACTGGCTGCGATCGTCTTC
TTCAACTACCACATCCTGGCCTCCCTGCGAACGCGGGAGGAGGTCAGCAGGACAAGGTGC
GGGGGCCGCAAGGATAGCAAGACCACAGCGCTGATCCTCACGCTCGTGGTTGCCTTCCTG
GTCTGCTGGGCCCCTTACCACTTCTTTGCCTTCCTGGAATTCTTATTCCAGGTGCAAGCA
GTCCGAGGCTGCTTTTGGGAGGACTTCATTGACCTGGGCCTGCAATTGGCCAACTTCTTT
GCCTTCACTAACAGCTCCCTGAATCCAGTAATTTATGTCTTTGTGGGCCGGCTCTTCAGG
ACCAAGGTCTGGGAACTTTATAAACAATGCACCCCTAAAAGTCTTGCTCCAATATCTTCA
TCCCATAGGAAAGAAATCTTCCAACTTTTCTGGCGGAAT

ORF Protein

Cloned ORF protein sequence for pFN21AE9529 Download

>KIEE9529  353 aa
MASSWPPLELQSSNQSQLFPQNATACDNAPEAWDLLHRVLPTFIISICFFGLLGNLFVLL
VFLLPRRQLNVAEIYLANLAASDLVFVLGLPFWAENIWNQFNWPFGALLCRVINGVIKAN
LFISIFLVVAISQDRYRVLVHPMASRRQQRRRQARVTCVLIWVVGGLLSIPTFLLRSIQA
VPDLNITACILLLPHEAWHFARIVELNILGFLLPLAAIVFFNYHILASLRTREEVSRTRC
GGRKDSKTTALILTLVVAFLVCWAPYHFFAFLEFLFQVQAVRGCFWEDFIDLGLQLANFF
AFTNSSLNPVIYVFVGRLFRTKVWELYKQCTPKSLAPISSSHRKEIFQLFWRN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9529 Download

>pFN21AE9529  5764 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCATCATCCTGGCCCCCTCTAGAGCTCCAATC
CTCCAACCAGAGCCAGCTCTTCCCTCAAAATGCTACGGCCTGTGACAATGCTCCAGAAGC
CTGGGACCTGCTGCACAGAGTGCTGCCGACATTTATCATCTCCATCTGTTTCTTCGGCCT
CCTAGGGAACCTTTTTGTCCTGTTGGTCTTCCTCCTGCCCCGGCGGCAACTGAACGTGGC
AGAAATCTACCTGGCCAACCTGGCAGCCTCTGATCTGGTGTTTGTCTTGGGCTTGCCCTT
CTGGGCAGAGAATATCTGGAACCAGTTTAACTGGCCTTTCGGAGCCCTCCTCTGCCGTGT
CATCAACGGGGTCATCAAGGCCAATTTGTTCATCAGCATCTTCCTGGTGGTGGCCATCAG
CCAGGACCGCTACCGCGTGCTGGTGCACCCTATGGCCAGCCGGAGGCAGCAGCGGCGGAG
GCAGGCCCGGGTCACCTGCGTGCTCATCTGGGTTGTGGGGGGCCTCTTGAGCATCCCCAC
ATTCCTGCTGCGATCCATCCAAGCCGTCCCAGATCTGAACATCACCGCCTGCATCCTGCT
CCTCCCCCATGAGGCCTGGCACTTTGCAAGGATTGTGGAGTTAAATATTCTGGGTTTCCT
CCTACCACTGGCTGCGATCGTCTTCTTCAACTACCACATCCTGGCCTCCCTGCGAACGCG
GGAGGAGGTCAGCAGGACAAGGTGCGGGGGCCGCAAGGATAGCAAGACCACAGCGCTGAT
CCTCACGCTCGTGGTTGCCTTCCTGGTCTGCTGGGCCCCTTACCACTTCTTTGCCTTCCT
GGAATTCTTATTCCAGGTGCAAGCAGTCCGAGGCTGCTTTTGGGAGGACTTCATTGACCT
GGGCCTGCAATTGGCCAACTTCTTTGCCTTCACTAACAGCTCCCTGAATCCAGTAATTTA
TGTCTTTGTGGGCCGGCTCTTCAGGACCAAGGTCTGGGAACTTTATAAACAATGCACCCC
TAAAAGTCTTGCTCCAATATCTTCATCCCATAGGAAAGAAATCTTCCAACTTTTCTGGCG
GAATGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT