ARHGAP1 ORF clone FHC08942

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC08942

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Clone info.

Species

human

Clone Name

pFN21AB6626 Sequence (with vector) Restriction Map

Product ID

FHC08942

Flexi Clone PID

FXC08942

ORF Sequence

DNA sequence (1317 bp)
Protein sequence (439 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528423]

Gene

KIBB6626

Symbol

ARHGAP1

Alias : CDC42GAP, RHOGAP, RHOGAP1, p50rhoGAP

Description

Rho GTPase activating protein 1

Original Clone ID

cs01226

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1317 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 439 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7922.1 2.5e-144 100.0 ARHGAP1 CCDS14060.2 1.3e-71 57.3 ARHGAP8 CCDS54538.2 1.6e-71 57.3 ARHGAP8 CCDS56233.1 2.6e-58 62.2 ARHGAP8 CCDS33664.1 8e-49 53.0 ARHGAP8 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001251 127 204 PF00650 Cellular retinaldehyde-binding/triple function IPR000198 260 405 PF00620 Rho GTPase-activating protein domain HMMSmart IPR001251 64 215 SM00516 Cellular retinaldehyde-binding/triple function IPR000198 257 428 SM00324 Rho GTPase-activating protein domain ProfileScan IPR001251 63 218 PS50191 Cellular retinaldehyde-binding/triple function IPR000198 244 431 PS50238 Rho GTPase-activating protein domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011518397 439 602732 100.0 100.0 NP_004299 439 602732 100.0 100.0 XP_016873209 395 602732 90.0 100.0 NP_851852 433 609405 57.3 86.8 NP_001017526 464 609405 53.0 86.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6626 Download

>KIBB6626  1317 bp
ATGGATCCGCTCTCAGAGCTGCAGGATGATCTGACCTTGGATGACACCAGCGAGGCTCTG
AACCAGCTGAAGCTGGCCTCCATCGATGAGAAGAACTGGCCCTCGGATGAAATGCCTGAC
TTCCCCAAGTCAGATGACTCCAAAAGCAGCTCCCCGGAACTTGTCACACACCTGAAGTGG
GATGACCCATACTATGACATCGCCCGGCACCAGATCGTGGAGGTGGCAGGAGATGACAAG
TATGGGCGGAAGATCATTGTGTTTAGTGCCTGTCGAATGCCCCCCAGCCACCAGCTCGAC
CACAGCAAGCTCCTGGGGTACCTGAAGCACACCCTGGACCAGTACGTGGAGAGTGACTAC
ACACTTCTGTATCTGCACCACGGCCTGACCAGCGACAACAAGCCCTCCCTCAGCTGGCTC
CGTGATGCCTACCGGGAGTTTGACCGCAAGTACAAGAAGAACATCAAGGCCTTGTACATC
GTGCATCCAACCATGTTCATCAAAACTCTGCTCATCCTCTTCAAGCCCCTCATCAGCTTC
AAGTTCGGGCAGAAGATCTTCTATGTGAATTACCTGAGCGAGCTGAGCGAGCACGTGAAG
CTGGAGCAGCTGGGGATCCCTCGCCAAGTGCTCAAATATGACGACTTCCTGAAATCCACA
CAGAAGAGCCCCGCGACAGCCCCCAAGCCCATGCCCCCACGGCCCCCCCTGCCCAACCAG
CAGTTTGGAGTCTCGCTGCAGCACCTCCAGGAGAAGAATCCAGAGCAGGAGCCCATTCCC
ATTGTACTCAGGGAGACTGTTGCCTACTTACAGGCCCACGCTCTCACCACCGAGGGCATC
TTCCGGAGGTCGGCCAACACCCAAGTGGTCCGGGAAGTGCAGCAGAAGTACAACATGGGG
CTGCCTGTGGATTTCGACCAGTACAATGAGCTGCACCTGCCAGCAGTCATCCTCAAGACC
TTCCTCCGGGAGCTTCCTGAGCCCCTGCTCACCTTTGACCTCTACCCCCATGTGGTGGGC
TTCCTCAACATTGATGAAAGCCAGAGGGTGCCAGCGACACTGCAGGTCCTCCAGACGCTG
CCCGAGGAGAACTACCAGGTGCTTCGTTTCCTGACTGCTTTCCTGGTGCAGATTTCTGCA
CACAGTGACCAGAACAAGATGACCAACACTAACCTGGCTGTTGTTTTCGGCCCTAACCTG
CTGTGGGCCAAGGATGCGGCCATCACCCTCAAGGCCATTAATCCCATCAACACCTTCACC
AAGTTCCTTCTGGATCACCAAGGGGAGCTGTTCCCAAGCCCGGACCCCAGCGGGCTC

ORF Protein

Cloned ORF protein sequence for pFN21AB6626 Download

>KIBB6626  439 aa
MDPLSELQDDLTLDDTSEALNQLKLASIDEKNWPSDEMPDFPKSDDSKSSSPELVTHLKW
DDPYYDIARHQIVEVAGDDKYGRKIIVFSACRMPPSHQLDHSKLLGYLKHTLDQYVESDY
TLLYLHHGLTSDNKPSLSWLRDAYREFDRKYKKNIKALYIVHPTMFIKTLLILFKPLISF
KFGQKIFYVNYLSELSEHVKLEQLGIPRQVLKYDDFLKSTQKSPATAPKPMPPRPPLPNQ
QFGVSLQHLQEKNPEQEPIPIVLRETVAYLQAHALTTEGIFRRSANTQVVREVQQKYNMG
LPVDFDQYNELHLPAVILKTFLRELPEPLLTFDLYPHVVGFLNIDESQRVPATLQVLQTL
PEENYQVLRFLTAFLVQISAHSDQNKMTNTNLAVVFGPNLLWAKDAAITLKAINPINTFT
KFLLDHQGELFPSPDPSGL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6626 Download

>pFN21AB6626  6022 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGATCCGCTCTCAGAGCTGCAGGATGATCTGAC
CTTGGATGACACCAGCGAGGCTCTGAACCAGCTGAAGCTGGCCTCCATCGATGAGAAGAA
CTGGCCCTCGGATGAAATGCCTGACTTCCCCAAGTCAGATGACTCCAAAAGCAGCTCCCC
GGAACTTGTCACACACCTGAAGTGGGATGACCCATACTATGACATCGCCCGGCACCAGAT
CGTGGAGGTGGCAGGAGATGACAAGTATGGGCGGAAGATCATTGTGTTTAGTGCCTGTCG
AATGCCCCCCAGCCACCAGCTCGACCACAGCAAGCTCCTGGGGTACCTGAAGCACACCCT
GGACCAGTACGTGGAGAGTGACTACACACTTCTGTATCTGCACCACGGCCTGACCAGCGA
CAACAAGCCCTCCCTCAGCTGGCTCCGTGATGCCTACCGGGAGTTTGACCGCAAGTACAA
GAAGAACATCAAGGCCTTGTACATCGTGCATCCAACCATGTTCATCAAAACTCTGCTCAT
CCTCTTCAAGCCCCTCATCAGCTTCAAGTTCGGGCAGAAGATCTTCTATGTGAATTACCT
GAGCGAGCTGAGCGAGCACGTGAAGCTGGAGCAGCTGGGGATCCCTCGCCAAGTGCTCAA
ATATGACGACTTCCTGAAATCCACACAGAAGAGCCCCGCGACAGCCCCCAAGCCCATGCC
CCCACGGCCCCCCCTGCCCAACCAGCAGTTTGGAGTCTCGCTGCAGCACCTCCAGGAGAA
GAATCCAGAGCAGGAGCCCATTCCCATTGTACTCAGGGAGACTGTTGCCTACTTACAGGC
CCACGCTCTCACCACCGAGGGCATCTTCCGGAGGTCGGCCAACACCCAAGTGGTCCGGGA
AGTGCAGCAGAAGTACAACATGGGGCTGCCTGTGGATTTCGACCAGTACAATGAGCTGCA
CCTGCCAGCAGTCATCCTCAAGACCTTCCTCCGGGAGCTTCCTGAGCCCCTGCTCACCTT
TGACCTCTACCCCCATGTGGTGGGCTTCCTCAACATTGATGAAAGCCAGAGGGTGCCAGC
GACACTGCAGGTCCTCCAGACGCTGCCCGAGGAGAACTACCAGGTGCTTCGTTTCCTGAC
TGCTTTCCTGGTGCAGATTTCTGCACACAGTGACCAGAACAAGATGACCAACACTAACCT
GGCTGTTGTTTTCGGCCCTAACCTGCTGTGGGCCAAGGATGCGGCCATCACCCTCAAGGC
CATTAATCCCATCAACACCTTCACCAAGTTCCTTCTGGATCACCAAGGGGAGCTGTTCCC
AAGCCCGGACCCCAGCGGGCTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT