ZNF256 ORF clone FHC07450

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Halotag ORF Clone FHC07450

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Clone info.

Species

human

Clone Name

pFN21AB7856 Sequence (with vector) Restriction Map

Product ID

FHC07450

Flexi Clone PID

FXC07450

ORF Sequence

DNA sequence (1881 bp)
Protein sequence (627 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464386]

Gene

KIBB7856

Symbol

ZNF256

Alias : BMZF-3, BMZF3

Description

zinc finger protein 256

Original Clone ID

cs00356

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1881 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 627 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12966.1 2.7e-134 100.0 ZNF256 CCDS42642.1 3.9e-72 57.7 ZNF418 CCDS82410.1 4e-72 57.7 ZNF418 CCDS12955.1 1e-67 56.0 ZNF530 CCDS12959.2 8.8e-67 54.2 ZNF551 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001909 14 54 PF01352 Krueppel-associated box IPR007087 267 289 PF00096 Zinc finger IPR007087 295 317 PF00096 Zinc finger IPR007087 323 345 PF00096 Zinc finger IPR007087 351 373 PF00096 Zinc finger IPR007087 491 513 PF00096 Zinc finger IPR007087 519 541 PF00096 Zinc finger IPR007087 547 569 PF00096 Zinc finger IPR007087 575 597 PF00096 Zinc finger IPR007087 603 625 PF00096 Zinc finger HMMSmart IPR001909 14 73 SM00349 Krueppel-associated box IPR015880 90 112 SM00355 Zinc finger IPR015880 239 261 SM00355 Zinc finger IPR015880 267 289 SM00355 Zinc finger IPR015880 295 317 SM00355 Zinc finger IPR015880 323 345 SM00355 Zinc finger IPR015880 351 373 SM00355 Zinc finger IPR015880 379 401 SM00355 Zinc finger IPR015880 407 429 SM00355 Zinc finger IPR015880 435 457 SM00355 Zinc finger IPR015880 463 485 SM00355 Zinc finger IPR015880 491 513 SM00355 Zinc finger IPR015880 519 541 SM00355 Zinc finger IPR015880 547 569 SM00355 Zinc finger IPR015880 575 597 SM00355 Zinc finger IPR015880 603 625 SM00355 Zinc finger ProfileScan IPR001909 14 96 PS50805 Krueppel-associated box IPR007087 90 117 PS50157 Zinc finger IPR007087 239 266 PS50157 Zinc finger IPR007087 267 294 PS50157 Zinc finger IPR007087 295 322 PS50157 Zinc finger IPR007087 323 350 PS50157 Zinc finger IPR007087 351 378 PS50157 Zinc finger IPR007087 379 406 PS50157 Zinc finger IPR007087 407 434 PS50157 Zinc finger IPR007087 435 462 PS50157 Zinc finger IPR007087 463 490 PS50157 Zinc finger IPR007087 491 518 PS50157 Zinc finger IPR007087 519 546 PS50157 Zinc finger IPR007087 547 574 PS50157 Zinc finger IPR007087 575 602 PS50157 Zinc finger IPR007087 603 627 PS50157 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005764 627 606956 100.0 100.0 NP_003424 706 604074 52.2 98.1 NP_942152 564 601856 59.3 87.1 NP_006376 577 601856 58.8 87.1 NP_001252529 503 601856 57.9 80.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7856 Download

>KIBB7856  1881 bp
ATGGCGGCGGCCGAGCTGACGGCCCCGGCCCAGGGCATTGTGACCTTTGAGGACGTGGCT
GTTTACTTCTCCTGGAAGGAATGGGGTCTTCTTGATGAGGCTCAGAAATGCCTGTACCAC
GATGTGATGCTGGAGAACTTGACACTTACAACCTCCCTGGGTGGTTCTGGAGCAGGGGAT
GAGGAGGCACCTTATCAGCAGAGCACTTCTCCACAGCGGGTGTCACAGGTTAGGATTCCT
AAGGCCCTTCCTTCTCCCCAGAAGACCAACCCCTGTGAGATATGTGGCCCAGTCTTGAGA
CAGATTTTGCACTTGGTTGAACACCAAGGAACACACCATGGTCAGAAACTGTATACAGAC
GGGGCATGTAGGAAACAATTACAATTTACTGCATACCTTCATCAGCACCAGAAGCAGCAT
GTTGGACAGAAACACTTCAGAAGCAATGGGGGCAGAGACATGTTTTTGAGCAGCTGCACA
TTTGAAGTATCTGGGAAGCCCTTCACTTGCAAGGAGGTTGGGAAGGATTTCCTGGTGAGA
TCAAGATTTCTTCAGCAACAGGCTGCTCACACCAGAAAGAAGTCAAACAGAACCAAGAGT
GCAGTGGCCTTTCACAGTGTAAAAAATCATTACAACTGGGGAGAATGTGTGAAAGCTTTC
AGCTACAAACATGTACGTGTTCAGCACCAGGGAGACCTCATTAGGGAAAGATCTTACATG
TGCAGTGAATGTGGGAAATCTTTTAGCACAAGCTGTAGCCTCAGTGATCATTTGAGAGTT
CACACTTCAGAAAAGCCTTATACATGTGGAGAATGTGGGAAATCCTATAGGCAGAGCTCT
AGCCTTATTACGCACCGAAGAATTCACACTGGAGTAAGACCTCATCAATGTGATGAATGT
GGAAAATTATTTAACAGGAAGTATGACCTTCTTATACATCAGAGAGTTCATACTGGAGAA
AGGCCTTACAAGTGCAGTGAATGTGGGAAATCCTTTAGCCATAGCTCTAGCCTCATTACA
CACCAGAGAATTCATACTGGAATGAGGCCTTATGAGTGCAGTGAATGTGGGAAATCTTTT
ATCCATAGTTCTAGCCTTATTACACACCAGAGAGTTCACACTGGTACAAGGCCTTATATG
TGCAGTGAATGTGGGAAATCCTTTAGCCAGAGCTGTCACCTCATTAAACACCGGAGACTT
CACATTGGAGAAGGGCCTTATGAGTGTAGTGAATGTGGGAAATTGTTTACTTATAGATCT
CGTTTCTTCCAACACCAGAGAGTTCATACTGGAGTAAGATCTCATGAATGTCATGAATGT
GGAAAATTATTTAGCAGGAAATTTGACCTCATTGTACATGAGAGAGTTCACACAGGAGAA
AGGCCATATGAGTGCAGTGAATGTGGAAAATCCTTTACCTGTAAATCCTACCTCATCTCA
CACTGGAAAGTTCATACTGGAGCAAGGCCTTATGAATGTGGGGAGTGTGGGAAATCATTT
ACTCATAGCTCTACGCTCCTTCAACACCAGAGAGTTCACACTGGAGAAAGGCCTTATGAG
TGCAATGAATGTGGGAAGTTTTTTAGCCAGAGCTCCAGCCTCATTAGACATAGGAGAAGT
CACACCGGAGAAAGGCCTTATGAGTGCAGTGAGTGTTGGAAATCCTTTAGTAACCACTCT
AGCCTCGTTAAACACCGAAGAGTTCATACCGGAGAAAGGCCTTATGAATGCAGTGAATGT
GGAAAATCCTTTAGCCAGAGCTCTAACCTCACTAATCACCAGCGAATTCACAGTGGGGAA
AGGCCTTATGAGTGTAGTGACTGTGGAAAATTTTTTACCTTCAACTCCAACCTCCTAAAA
CATCAGAACGTTCACAAGGGA

ORF Protein

Cloned ORF protein sequence for pFN21AB7856 Download

>KIBB7856  627 aa
MAAAELTAPAQGIVTFEDVAVYFSWKEWGLLDEAQKCLYHDVMLENLTLTTSLGGSGAGD
EEAPYQQSTSPQRVSQVRIPKALPSPQKTNPCEICGPVLRQILHLVEHQGTHHGQKLYTD
GACRKQLQFTAYLHQHQKQHVGQKHFRSNGGRDMFLSSCTFEVSGKPFTCKEVGKDFLVR
SRFLQQQAAHTRKKSNRTKSAVAFHSVKNHYNWGECVKAFSYKHVRVQHQGDLIRERSYM
CSECGKSFSTSCSLSDHLRVHTSEKPYTCGECGKSYRQSSSLITHRRIHTGVRPHQCDEC
GKLFNRKYDLLIHQRVHTGERPYKCSECGKSFSHSSSLITHQRIHTGMRPYECSECGKSF
IHSSSLITHQRVHTGTRPYMCSECGKSFSQSCHLIKHRRLHIGEGPYECSECGKLFTYRS
RFFQHQRVHTGVRSHECHECGKLFSRKFDLIVHERVHTGERPYECSECGKSFTCKSYLIS
HWKVHTGARPYECGECGKSFTHSSTLLQHQRVHTGERPYECNECGKFFSQSSSLIRHRRS
HTGERPYECSECWKSFSNHSSLVKHRRVHTGERPYECSECGKSFSQSSNLTNHQRIHSGE
RPYECSDCGKFFTFNSNLLKHQNVHKG

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7856 Download

>pFN21AB7856  6586 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCGGCCGAGCTGACGGCCCCGGCCCAGGG
CATTGTGACCTTTGAGGACGTGGCTGTTTACTTCTCCTGGAAGGAATGGGGTCTTCTTGA
TGAGGCTCAGAAATGCCTGTACCACGATGTGATGCTGGAGAACTTGACACTTACAACCTC
CCTGGGTGGTTCTGGAGCAGGGGATGAGGAGGCACCTTATCAGCAGAGCACTTCTCCACA
GCGGGTGTCACAGGTTAGGATTCCTAAGGCCCTTCCTTCTCCCCAGAAGACCAACCCCTG
TGAGATATGTGGCCCAGTCTTGAGACAGATTTTGCACTTGGTTGAACACCAAGGAACACA
CCATGGTCAGAAACTGTATACAGACGGGGCATGTAGGAAACAATTACAATTTACTGCATA
CCTTCATCAGCACCAGAAGCAGCATGTTGGACAGAAACACTTCAGAAGCAATGGGGGCAG
AGACATGTTTTTGAGCAGCTGCACATTTGAAGTATCTGGGAAGCCCTTCACTTGCAAGGA
GGTTGGGAAGGATTTCCTGGTGAGATCAAGATTTCTTCAGCAACAGGCTGCTCACACCAG
AAAGAAGTCAAACAGAACCAAGAGTGCAGTGGCCTTTCACAGTGTAAAAAATCATTACAA
CTGGGGAGAATGTGTGAAAGCTTTCAGCTACAAACATGTACGTGTTCAGCACCAGGGAGA
CCTCATTAGGGAAAGATCTTACATGTGCAGTGAATGTGGGAAATCTTTTAGCACAAGCTG
TAGCCTCAGTGATCATTTGAGAGTTCACACTTCAGAAAAGCCTTATACATGTGGAGAATG
TGGGAAATCCTATAGGCAGAGCTCTAGCCTTATTACGCACCGAAGAATTCACACTGGAGT
AAGACCTCATCAATGTGATGAATGTGGAAAATTATTTAACAGGAAGTATGACCTTCTTAT
ACATCAGAGAGTTCATACTGGAGAAAGGCCTTACAAGTGCAGTGAATGTGGGAAATCCTT
TAGCCATAGCTCTAGCCTCATTACACACCAGAGAATTCATACTGGAATGAGGCCTTATGA
GTGCAGTGAATGTGGGAAATCTTTTATCCATAGTTCTAGCCTTATTACACACCAGAGAGT
TCACACTGGTACAAGGCCTTATATGTGCAGTGAATGTGGGAAATCCTTTAGCCAGAGCTG
TCACCTCATTAAACACCGGAGACTTCACATTGGAGAAGGGCCTTATGAGTGTAGTGAATG
TGGGAAATTGTTTACTTATAGATCTCGTTTCTTCCAACACCAGAGAGTTCATACTGGAGT
AAGATCTCATGAATGTCATGAATGTGGAAAATTATTTAGCAGGAAATTTGACCTCATTGT
ACATGAGAGAGTTCACACAGGAGAAAGGCCATATGAGTGCAGTGAATGTGGAAAATCCTT
TACCTGTAAATCCTACCTCATCTCACACTGGAAAGTTCATACTGGAGCAAGGCCTTATGA
ATGTGGGGAGTGTGGGAAATCATTTACTCATAGCTCTACGCTCCTTCAACACCAGAGAGT
TCACACTGGAGAAAGGCCTTATGAGTGCAATGAATGTGGGAAGTTTTTTAGCCAGAGCTC
CAGCCTCATTAGACATAGGAGAAGTCACACCGGAGAAAGGCCTTATGAGTGCAGTGAGTG
TTGGAAATCCTTTAGTAACCACTCTAGCCTCGTTAAACACCGAAGAGTTCATACCGGAGA
AAGGCCTTATGAATGCAGTGAATGTGGAAAATCCTTTAGCCAGAGCTCTAACCTCACTAA
TCACCAGCGAATTCACAGTGGGGAAAGGCCTTATGAGTGTAGTGACTGTGGAAAATTTTT
TACCTTCAACTCCAACCTCCTAAAACATCAGAACGTTCACAAGGGAGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT