SLC35E4 ORF clone FHC06871

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Halotag ORF Clone FHC06871

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Clone info.

Species

human

Clone Name

pFN21AE0520 Sequence (with vector) Restriction Map

Product ID

FHC06871

Flexi Clone PID

FXC06871

ORF Sequence

DNA sequence (1050 bp)
Protein sequence (350 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528237]

Gene

KIEE0520

Symbol

SLC35E4

Description

solute carrier family 35, member E4

Original Clone ID

oc01220

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1050 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 350 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13882.1 8.9e-132 100.0 SLC35E4 CCDS82708.1 3.1e-78 96.3 SLC35E4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000620 80 177 PF00892 Drug/metabolite transporter IPR004853 191 326 PF03151 Domain of unknown function DUF250
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 48 RVAMAALVWLLAGASMSSLNKWI 70 SECONDARY 23 2 73 VHGFGRPLLLSALHMLVAALACH 95 SECONDARY 23 3 135 LAQLVTTTTPLFTLALSALLLG 156 SECONDARY 22 4 221 LYATSLPSFCLLAGAALVLEAGV 243 SECONDARY 23 5 254 LWACILLSCLLSVLYNLASFSLL 276 PRIMARY 23 6 303 FGSRLSALSYVGIALTLSGMFLY 325 SECONDARY 23 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0520 Download

>KIEE0520  1050 bp
ATGTGCCGCTGCCCGCCGGAGCACCATGATGGCAGGATGACCTCAGCCGAAGTAGGAGCA
GCAGCTGGTGGTGCTCAGGCGGCTGGGCCCCCCGAGTGGCCCCCTGGCAGCCCTCAGGCC
CTCCGGCAGCCTGGCCGGGCCCGAGTGGCCATGGCAGCACTGGTGTGGCTGCTGGCGGGA
GCCAGCATGTCAAGCCTCAACAAGTGGATCTTCACAGTGCACGGCTTTGGGCGGCCCCTG
CTGCTGTCGGCCCTGCACATGCTGGTGGCAGCCCTGGCATGCCACCGGGGGGCACGGCGC
CCCATGCCAGGCGGCACTCGCTGCCGAGTCCTACTGCTCAGTCTCACCTTTGGCACGTCC
ATGGCCTGCGGCAACGTGGGCCTAAGGGCTGTGCCCCTGGACCTGGCACAACTGGTTACT
ACCACCACACCTCTGTTCACCCTGGCCCTGTCGGCGCTGCTGCTGGGCCGCCGCCACCAC
CCGCTTCAGTTGGCCGCCATGGGTCCGCTCTGCCTGGGGGCCGCCTGCAGCCTGGCTGGA
GAGTTCCGGACACCCCCTACCGGCTGTGGCTTCCTGCTCGCAGCCACCTGCCTCCGCGGA
CTCAAGTCGGTTCAGCAAAGTGCCCTGCTGCAGGAGGAGAGGCTGGACGCGGTGACCCTG
CTTTACGCCACCTCGCTGCCCAGCTTCTGCCTGCTGGCGGGTGCAGCCCTGGTGCTGGAG
GCTGGCGTTGCCCCACCGCCCACTGCTGGCGACTCTCGCCTCTGGGCCTGCATCCTGCTC
AGCTGCCTCCTGTCTGTTCTCTATAACCTGGCCAGCTTCTCCCTGCTGGCCCTCACCTCT
GCCCTCACCGTCCACGTCCTGGGCAACCTCACCGTGGTGGGCAACCTCATCCTGTCCCGG
CTGTTGTTTGGCAGCCGCCTCAGTGCCCTCAGCTACGTGGGCATCGCACTCACTCTTTCA
GGAATGTTCCTTTACCACAACTGCGAGTTCGTGGCCTCCTGGGCTGCCCGTCGGGGGCTG
TGGCGGAGGGACCAGCCCAGCAAGGGTCTT

ORF Protein

Cloned ORF protein sequence for pFN21AE0520 Download

>KIEE0520  350 aa
MCRCPPEHHDGRMTSAEVGAAAGGAQAAGPPEWPPGSPQALRQPGRARVAMAALVWLLAG
ASMSSLNKWIFTVHGFGRPLLLSALHMLVAALACHRGARRPMPGGTRCRVLLLSLTFGTS
MACGNVGLRAVPLDLAQLVTTTTPLFTLALSALLLGRRHHPLQLAAMGPLCLGAACSLAG
EFRTPPTGCGFLLAATCLRGLKSVQQSALLQEERLDAVTLLYATSLPSFCLLAGAALVLE
AGVAPPPTAGDSRLWACILLSCLLSVLYNLASFSLLALTSALTVHVLGNLTVVGNLILSR
LLFGSRLSALSYVGIALTLSGMFLYHNCEFVASWAARRGLWRRDQPSKGL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0520 Download

>pFN21AE0520  5755 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTGCCGCTGCCCGCCGGAGCACCATGATGGCAG
GATGACCTCAGCCGAAGTAGGAGCAGCAGCTGGTGGTGCTCAGGCGGCTGGGCCCCCCGA
GTGGCCCCCTGGCAGCCCTCAGGCCCTCCGGCAGCCTGGCCGGGCCCGAGTGGCCATGGC
AGCACTGGTGTGGCTGCTGGCGGGAGCCAGCATGTCAAGCCTCAACAAGTGGATCTTCAC
AGTGCACGGCTTTGGGCGGCCCCTGCTGCTGTCGGCCCTGCACATGCTGGTGGCAGCCCT
GGCATGCCACCGGGGGGCACGGCGCCCCATGCCAGGCGGCACTCGCTGCCGAGTCCTACT
GCTCAGTCTCACCTTTGGCACGTCCATGGCCTGCGGCAACGTGGGCCTAAGGGCTGTGCC
CCTGGACCTGGCACAACTGGTTACTACCACCACACCTCTGTTCACCCTGGCCCTGTCGGC
GCTGCTGCTGGGCCGCCGCCACCACCCGCTTCAGTTGGCCGCCATGGGTCCGCTCTGCCT
GGGGGCCGCCTGCAGCCTGGCTGGAGAGTTCCGGACACCCCCTACCGGCTGTGGCTTCCT
GCTCGCAGCCACCTGCCTCCGCGGACTCAAGTCGGTTCAGCAAAGTGCCCTGCTGCAGGA
GGAGAGGCTGGACGCGGTGACCCTGCTTTACGCCACCTCGCTGCCCAGCTTCTGCCTGCT
GGCGGGTGCAGCCCTGGTGCTGGAGGCTGGCGTTGCCCCACCGCCCACTGCTGGCGACTC
TCGCCTCTGGGCCTGCATCCTGCTCAGCTGCCTCCTGTCTGTTCTCTATAACCTGGCCAG
CTTCTCCCTGCTGGCCCTCACCTCTGCCCTCACCGTCCACGTCCTGGGCAACCTCACCGT
GGTGGGCAACCTCATCCTGTCCCGGCTGTTGTTTGGCAGCCGCCTCAGTGCCCTCAGCTA
CGTGGGCATCGCACTCACTCTTTCAGGAATGTTCCTTTACCACAACTGCGAGTTCGTGGC
CTCCTGGGCTGCCCGTCGGGGGCTGTGGCGGAGGGACCAGCCCAGCAAGGGTCTTGTTTA
AACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTG
ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAAT
AACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAG
TTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGAT
GCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC
ATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAAC
CTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTT
GTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCT
TTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACC
CCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCC
TGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTC
GCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTG
GTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGAT
CTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGC
ACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA
CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA
AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGT
GATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCT
TTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT
GAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTG
CGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGG
ATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTT
ATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGG
CCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG
GATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTC
GAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGT
TATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGG
CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACG
AGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT
ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA
CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCT
GTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCC
CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA
GACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATG
TAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG
TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT
GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTA
CGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTC
AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCA
CCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGA
TGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTAC
CCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTT
TCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTA
GCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATC
GAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT