LIMS2 ORF clone FHC05837

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC05837

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Clone info.

Species

human

Clone Name

pFN21AB6527 Sequence (with vector) Restriction Map

Product ID

FHC05837

Flexi Clone PID

FXC05837

ORF Sequence

DNA sequence (1095 bp)
Protein sequence (365 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463832]

Gene

KIBB6527

Symbol

LIMS2

Alias : LGMD2W, MDRCMTT, PINCH-2, PINCH2

Description

LIM and senescent cell antigen-like domains 2, transcript variant 2

Original Clone ID

cs01127

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1095 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 365 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2147.1 1.1e-96 100.0 LIMS2 CCDS54395.1 1.4e-89 99.7 LIMS2 CCDS54396.1 1.6e-89 100.0 LIMS2 CCDS54394.1 2.7e-89 100.0 LIMS2 CCDS54384.1 9.7e-79 80.8 LIMS1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001781 39 95 PF00412 Zinc finger IPR001781 100 154 PF00412 Zinc finger IPR001781 164 215 PF00412 Zinc finger IPR001781 222 276 PF00412 Zinc finger IPR001781 281 335 PF00412 Zinc finger HMMSmart IPR001781 38 91 SM00132 Zinc finger IPR001781 99 150 SM00132 Zinc finger IPR001781 163 213 SM00132 Zinc finger IPR001781 221 272 SM00132 Zinc finger IPR001781 280 332 SM00132 Zinc finger ProfileScan IPR001781 37 98 PS50023 Zinc finger IPR001781 100 157 PS50023 Zinc finger IPR001781 162 219 PS50023 Zinc finger IPR001781 220 279 PS50023 Zinc finger IPR001781 280 339 PS50023 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060450 365 607908,616827 100.0 100.0 NP_001154875 341 607908,616827 99.7 92.9 NP_001129509 363 607908,616827 100.0 92.3 NP_001154876 336 607908,616827 100.0 92.1 NP_001180413 362 602567 80.8 96.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6527 Download

>KIBB6527  1095 bp
ATGGCGGCCCGGCTGGGTGCGCTGGCCGCGTCGGGGCTGTACCGGCGGCGCCAGCACCGG
CAGAGCCCGCCGCCAGCCACGGGCAATATGTCGGACGCCTTGGCCAACGCCGTGTGCCAG
CGCTGCCAGGCCCGCTTCTCCCCCGCCGAGCGCATTGTCAACAGCAATGGGGAGCTGTAC
CATGAGCACTGCTTCGTGTGTGCCCAGTGCTTCCGGCCCTTCCCCGAGGGGCTCTTCTAT
GAGTTTGAAGGCCGGAAGTACTGCGAACACGACTTCCAAATGCTGTTTGCTCCGTGCTGT
GGATCCTGCGGTGAGTTCATCATTGGCCGCGTCATCAAGGCCATGAACAACAACTGGCAC
CCGGGCTGCTTCCGCTGCGAGCTGTGTGATGTGGAGCTGGCTGACCTGGGCTTTGTGAAG
AATGCCGGCAGGCATCTCTGCCGGCCTTGCCACAACCGTGAGAAGGCCAAGGGCCTGGGC
AAGTACATCTGCCAGCGGTGCCACCTGGTCATCGACGAGCAGCCCCTCATGTTCAGGAGC
GACGCCTACCACCCTGACCACTTCAACTGCACCCACTGTGGGAAGGAGCTGACAGCCGAG
GCCCGCGAGCTGAAGGGTGAGCTCTACTGCCTGCCCTGCCATGACAAGATGGGCGTCCCC
ATCTGCGGGGCCTGTCGCCGGCCCATCGAGGGCCGAGTGGTCAACGCGCTGGGCAAGCAG
TGGCACGTGGAGCACTTTGTCTGTGCCAAGTGTGAGAAGCCATTCCTGGGGCACCGGCAC
TATGAGAAGAAGGGCCTGGCCTACTGCGAGACTCACTACAACCAGCTCTTCGGGGACGTC
TGCTACAACTGCAGCCATGTGATTGAAGGCGATGTGGTGTCGGCCCTCAACAAGGCCTGG
TGTGTGAGCTGCTTCTCCTGCTCCACCTGCAACAGCAAGCTCACCCTGAAGAACAAGTTT
GTGGAGTTCGACATGAAGCCCGTGTGTAAGAGGTGCTACGAGAAGTTCCCGCTGGAGCTG
AAGAAGCGGCTGAAGAAGCTGTCGGAGCTGACCTCCCGCAAGGCCCAGCCCAAGGCCACA
GACCTCAACTCTGCC

ORF Protein

Cloned ORF protein sequence for pFN21AB6527 Download

>KIBB6527  365 aa
MAARLGALAASGLYRRRQHRQSPPPATGNMSDALANAVCQRCQARFSPAERIVNSNGELY
HEHCFVCAQCFRPFPEGLFYEFEGRKYCEHDFQMLFAPCCGSCGEFIIGRVIKAMNNNWH
PGCFRCELCDVELADLGFVKNAGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRS
DAYHPDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQ
WHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAW
CVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKAT
DLNSA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6527 Download

>pFN21AB6527  5800 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCCCGGCTGGGTGCGCTGGCCGCGTCGGG
GCTGTACCGGCGGCGCCAGCACCGGCAGAGCCCGCCGCCAGCCACGGGCAATATGTCGGA
CGCCTTGGCCAACGCCGTGTGCCAGCGCTGCCAGGCCCGCTTCTCCCCCGCCGAGCGCAT
TGTCAACAGCAATGGGGAGCTGTACCATGAGCACTGCTTCGTGTGTGCCCAGTGCTTCCG
GCCCTTCCCCGAGGGGCTCTTCTATGAGTTTGAAGGCCGGAAGTACTGCGAACACGACTT
CCAAATGCTGTTTGCTCCGTGCTGTGGATCCTGCGGTGAGTTCATCATTGGCCGCGTCAT
CAAGGCCATGAACAACAACTGGCACCCGGGCTGCTTCCGCTGCGAGCTGTGTGATGTGGA
GCTGGCTGACCTGGGCTTTGTGAAGAATGCCGGCAGGCATCTCTGCCGGCCTTGCCACAA
CCGTGAGAAGGCCAAGGGCCTGGGCAAGTACATCTGCCAGCGGTGCCACCTGGTCATCGA
CGAGCAGCCCCTCATGTTCAGGAGCGACGCCTACCACCCTGACCACTTCAACTGCACCCA
CTGTGGGAAGGAGCTGACAGCCGAGGCCCGCGAGCTGAAGGGTGAGCTCTACTGCCTGCC
CTGCCATGACAAGATGGGCGTCCCCATCTGCGGGGCCTGTCGCCGGCCCATCGAGGGCCG
AGTGGTCAACGCGCTGGGCAAGCAGTGGCACGTGGAGCACTTTGTCTGTGCCAAGTGTGA
GAAGCCATTCCTGGGGCACCGGCACTATGAGAAGAAGGGCCTGGCCTACTGCGAGACTCA
CTACAACCAGCTCTTCGGGGACGTCTGCTACAACTGCAGCCATGTGATTGAAGGCGATGT
GGTGTCGGCCCTCAACAAGGCCTGGTGTGTGAGCTGCTTCTCCTGCTCCACCTGCAACAG
CAAGCTCACCCTGAAGAACAAGTTTGTGGAGTTCGACATGAAGCCCGTGTGTAAGAGGTG
CTACGAGAAGTTCCCGCTGGAGCTGAAGAAGCGGCTGAAGAAGCTGTCGGAGCTGACCTC
CCGCAAGGCCCAGCCCAAGGCCACAGACCTCAACTCTGCCGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT