BRF2 ORF clone FHC04286

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC04286

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Clone info.

Species

human

Clone Name

pFN21AB7707 Sequence (with vector) Restriction Map

Product ID

FHC04286

Flexi Clone PID

FXC04286

ORF Sequence

DNA sequence (1257 bp)
Protein sequence (419 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463449]

Gene

KIBB7707

Symbol

BRF2

Alias : BRFU, TFIIIB50

Description

BRF2, RNA polymerase III transcription initiation factor 50 kDa subunit

Original Clone ID

cs00207

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1257 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 419 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6098.1 4.4e-155 100.0 BRF2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013137 6 37 PF08271 Zinc finger ProfileScan IPR013137 2 36 PS51134 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060780 419 607013 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7707 Download

>KIBB7707  1257 bp
ATGCCAGGCAGAGGCCGCTGCCCGGACTGCGGCTCCACGGAGCTGGTGGAAGACTCGCAC
TATTCGCAGAGCCAGCTGGTGTGCTCCGACTGCGGCTGCGTGGTCACCGAGGGGGTCCTT
ACCACTACCTTCAGCGACGAGGGCAATCTCCGAGAGGTAACATATTCCCGAAGCACAGGG
GAAAACGAACAAGTTAGTCGCAGCCAGCAACGAGGTCTCCGGCGAGTGAGAGACCTTTGT
CGAGTTCTGCAGTTGCCACCAACATTTGAGGATACCGCGGTTGCCTACTACCAACAGGCA
TATCGGCACTCTGGCATCCGAGCGGCCAGGCTGCAAAAGAAGGAGGTGTTGGTTGGGTGC
TGCGTCTTAATCACCTGCCGACAGCATAACTGGCCCCTAACAATGGGGGCCATCTGCACG
CTGTTGTATGCAGATTTGGATGTGTTTTCTAGCACTTACATGCAGATAGTGAAGCTCCTG
GGACTGGATGTGCCATCTCTGTGCTTGGCAGAACTGGTGAAGACCTATTGCAGCAGCTTC
AAACTGTTCCAAGCTTCACCTTCTGTGCCAGCCAAATACGTGGAAGACAAAGAGAAGATG
CTGTCTCGAACAATGCAGTTGGTGGAGCTGGCAAATGAGACGTGGCTGGTGACCGGGAGG
CATCCCTTGCCCGTCATCACTGCTGCGACTTTCCTGGCTTGGCAGTCGCTGCAGCCTGCA
GATCGGCTTTCATGTTCCCTTGCCCGATTTTGTAAATTGGCAAATGTGGACCTGCCCTAC
CCGGCGTCCTCCCGCCTGCAGGAGCTGCTGGCTGTGCTGCTGCGGATGGCTGAGCAGCTG
GCCTGGTTACGAGTTCTGAGACTTGACAAACGGTCTGTGGTGAAGCACATCGGTGACCTT
CTCCAGCACCGCCAGTCACTGGTCCGCTCTGCCTTTCGGGATGGGACAGCAGAAGTGGAG
ACCCGAGAGAAGGAGCCACCGGGGTGGGGACAGGGGCAAGGAGAAGGGGAGGTGGGAAAT
AATTCCTTAGGTTTACCCCAGGGGAAGCGGCCGGCCAGTCCTGCCCTTCTCTTGCCACCC
TGCATGTTGAAGTCCCCGAAGCGGATCTGCCCTGTACCCCCTGTCTCCACTGTCACTGGA
GATGAGAACATTTCTGATAGTGAAATAGAACAGTATTTGCGTACCCCTCAGGAAGTTAGG
GACTTTCAGAGAGCCCAGGCTGCTAGACAGGCTGCCACGAGTGTCCCTAACCCTCCC

ORF Protein

Cloned ORF protein sequence for pFN21AB7707 Download

>KIBB7707  419 aa
MPGRGRCPDCGSTELVEDSHYSQSQLVCSDCGCVVTEGVLTTTFSDEGNLREVTYSRSTG
ENEQVSRSQQRGLRRVRDLCRVLQLPPTFEDTAVAYYQQAYRHSGIRAARLQKKEVLVGC
CVLITCRQHNWPLTMGAICTLLYADLDVFSSTYMQIVKLLGLDVPSLCLAELVKTYCSSF
KLFQASPSVPAKYVEDKEKMLSRTMQLVELANETWLVTGRHPLPVITAATFLAWQSLQPA
DRLSCSLARFCKLANVDLPYPASSRLQELLAVLLRMAEQLAWLRVLRLDKRSVVKHIGDL
LQHRQSLVRSAFRDGTAEVETREKEPPGWGQGQGEGEVGNNSLGLPQGKRPASPALLLPP
CMLKSPKRICPVPPVSTVTGDENISDSEIEQYLRTPQEVRDFQRAQAARQAATSVPNPP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7707 Download

>pFN21AB7707  5962 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCAGGCAGAGGCCGCTGCCCGGACTGCGGCTC
CACGGAGCTGGTGGAAGACTCGCACTATTCGCAGAGCCAGCTGGTGTGCTCCGACTGCGG
CTGCGTGGTCACCGAGGGGGTCCTTACCACTACCTTCAGCGACGAGGGCAATCTCCGAGA
GGTAACATATTCCCGAAGCACAGGGGAAAACGAACAAGTTAGTCGCAGCCAGCAACGAGG
TCTCCGGCGAGTGAGAGACCTTTGTCGAGTTCTGCAGTTGCCACCAACATTTGAGGATAC
CGCGGTTGCCTACTACCAACAGGCATATCGGCACTCTGGCATCCGAGCGGCCAGGCTGCA
AAAGAAGGAGGTGTTGGTTGGGTGCTGCGTCTTAATCACCTGCCGACAGCATAACTGGCC
CCTAACAATGGGGGCCATCTGCACGCTGTTGTATGCAGATTTGGATGTGTTTTCTAGCAC
TTACATGCAGATAGTGAAGCTCCTGGGACTGGATGTGCCATCTCTGTGCTTGGCAGAACT
GGTGAAGACCTATTGCAGCAGCTTCAAACTGTTCCAAGCTTCACCTTCTGTGCCAGCCAA
ATACGTGGAAGACAAAGAGAAGATGCTGTCTCGAACAATGCAGTTGGTGGAGCTGGCAAA
TGAGACGTGGCTGGTGACCGGGAGGCATCCCTTGCCCGTCATCACTGCTGCGACTTTCCT
GGCTTGGCAGTCGCTGCAGCCTGCAGATCGGCTTTCATGTTCCCTTGCCCGATTTTGTAA
ATTGGCAAATGTGGACCTGCCCTACCCGGCGTCCTCCCGCCTGCAGGAGCTGCTGGCTGT
GCTGCTGCGGATGGCTGAGCAGCTGGCCTGGTTACGAGTTCTGAGACTTGACAAACGGTC
TGTGGTGAAGCACATCGGTGACCTTCTCCAGCACCGCCAGTCACTGGTCCGCTCTGCCTT
TCGGGATGGGACAGCAGAAGTGGAGACCCGAGAGAAGGAGCCACCGGGGTGGGGACAGGG
GCAAGGAGAAGGGGAGGTGGGAAATAATTCCTTAGGTTTACCCCAGGGGAAGCGGCCGGC
CAGTCCTGCCCTTCTCTTGCCACCCTGCATGTTGAAGTCCCCGAAGCGGATCTGCCCTGT
ACCCCCTGTCTCCACTGTCACTGGAGATGAGAACATTTCTGATAGTGAAATAGAACAGTA
TTTGCGTACCCCTCAGGAAGTTAGGGACTTTCAGAGAGCCCAGGCTGCTAGACAGGCTGC
CACGAGTGTCCCTAACCCTCCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT