VPS33A ORF clone FHC03590

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC03590

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Clone info.

Species

human

Clone Name

pFN21AB6151 Sequence (with vector) Restriction Map

Product ID

FHC03590

Flexi Clone PID

FXC03590

ORF Sequence

DNA sequence (1788 bp)
Protein sequence (596 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527663]

Gene

KIBB6151

Symbol

VPS33A

Alias : MPSPS

Description

vacuolar protein sorting 33 homolog A (S. cerevisiae)

Original Clone ID

cs00751

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1788 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 596 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9231.1 0 100.0 VPS33A CCDS10369.1 6.3e-31 31.6 VPS33B CCDS73783.1 1.4e-26 31.9 VPS33B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001619 34 591 PF00995 Sec1-like protein
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_075067 596 610034 100.0 100.0 NP_061138 617 208085,608552 31.6 97.8 NP_001276077 590 208085,608552 31.9 90.9 XP_011519750 526 208085,608552 31.9 84.6 NP_001276078 526 208085,608552 31.9 84.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6151 Download

>KIBB6151  1788 bp
ATGGCGGCTCATCTGTCCTACGGCCGAGTGAACCTAAACGTGTTGCGCGAGGCGGTGCGT
CGCGAGCTGCGCGAGTTCCTGGACAAGTGCGCAGGAAGCAAGGCAATAGTTTGGGATGAA
TACCTAACTGGACCCTTTGGCCTGATTGCACAGTATTCACTATTGAAGGAACATGAAGTG
GAAAAAATGTTCACACTTAAAGGAAATCGTTTGCCGGCAGCTGATGTGAAGAATATAATT
TTTTTTGTCAGACCCAGGCTAGAGTTGATGGATATAATCGCTGAAAACGTGCTCAGTGAA
GATAGACGAGGCCCAACGAGAGATTTTCATATTCTGTTTGTGCCACGCCGTAGCCTGTTG
TGCGAACAGCGGTTGAAGGATCTGGGTGTCTTGGGATCCTTTATTCACAGGGAGGAGTAC
AGCTTAGATCTCATTCCATTCGATGGGGATCTCTTATCCATGGAATCAGAGGGTGCATTC
AAAGAGTGCTACCTGGAGGGTGACCAGACGAGCCTGTACCACGCAGCCAAGGGGCTGATG
ACCCTGCAAGCTCTGTATGGAACGATCCCCCAGATCTTTGGGAAAGGAGAATGCGCTCGG
CAAGTGGCCAATATGATGATCAGGATGAAGAGAGAGTTTACAGGAAGCCAGAATTCAATA
TTTCCTGTTTTTGATAATCTCTTGTTGCTTGATCGGAATGTGGATTTATTAACACCTCTT
GCCACTCAGCTGACATATGAAGGACTCATTGATGAAATTTATGGCATTCAGAACAGTTAT
GTGAAATTACCTCCAGAGAAATTTGCACCTAAGAAACAGGGCGATGGTGGTAAGGACCTC
CCCACGGAAGCAAAGAAGCTGCAGCTGAATTCTGCAGAGGAGCTCTATGCTGAGATCCGA
GATAAGAACTTCAACGCAGTTGGCTCTGTGCTCAGCAAGAAAGCAAAGATCATCTCTGCA
GCATTCGAGGAAAGACACAATGCTAAGACCGTGGGGGAGATCAAGCAGTTTGTTTCCCAG
TTGCCCCACATGCAGGCAGCAAGGGGCTCGCTTGCAAACCATACCTCAATTGCAGAATTG
ATCAAAGATGTCACTACTTCTGAAGACTTTTTTGATAAATTAACCGTGGAACAGGAGTTT
ATGTCTGGAATAGACACTGATAAGGTCAACAATTACATTGAGGATTGTATCGCCCAAAAG
CACTCGTTGATCAAGGTGTTAAGACTAGTTTGCCTCCAATCCGTGTGTAATAGTGGGCTC
AAACAAAAAGTTTTGGATTATTACAAAAGAGAGATTCTCCAGACATACGGCTATGAGCAC
ATATTGACCTTACACAACCTGGAGAAGGCCGGCCTGCTGAAACCGCAGACGGGGGGCAGA
AACAATTACCCAACTATACGGAAAACATTACGCCTCTGGATGGATGATGTTAATGAGCAA
AACCCCACGGACATATCGTATGTGTACAGTGGGTATGCCCCGCTCAGTGTGCGGCTGGCC
CAGCTGCTTTCCCGGCCTGGCTGGCGGAGCATCGAGGAGGTCCTCCGCATCCTCCCAGGG
CCCCACTTTGAGGAGCGGCAGCCACTGCCCACAGGACTGCAGAAGAAACGTCAACCGGGA
GAAAACCGAGTGACTCTGATATTTTTCCTTGGGGGCGTAACCTTCGCTGAAATTGCTGCC
CTGCGATTTCTCTCCCAGTTGGAAGATGGAGGTACAGAATATGTCATTGCCACCACTAAA
CTAATGAATGGAACCAGTTGGATAGAGGCTCTGATGGAAAAACCTTTC

ORF Protein

Cloned ORF protein sequence for pFN21AB6151 Download

>KIBB6151  596 aa
MAAHLSYGRVNLNVLREAVRRELREFLDKCAGSKAIVWDEYLTGPFGLIAQYSLLKEHEV
EKMFTLKGNRLPAADVKNIIFFVRPRLELMDIIAENVLSEDRRGPTRDFHILFVPRRSLL
CEQRLKDLGVLGSFIHREEYSLDLIPFDGDLLSMESEGAFKECYLEGDQTSLYHAAKGLM
TLQALYGTIPQIFGKGECARQVANMMIRMKREFTGSQNSIFPVFDNLLLLDRNVDLLTPL
ATQLTYEGLIDEIYGIQNSYVKLPPEKFAPKKQGDGGKDLPTEAKKLQLNSAEELYAEIR
DKNFNAVGSVLSKKAKIISAAFEERHNAKTVGEIKQFVSQLPHMQAARGSLANHTSIAEL
IKDVTTSEDFFDKLTVEQEFMSGIDTDKVNNYIEDCIAQKHSLIKVLRLVCLQSVCNSGL
KQKVLDYYKREILQTYGYEHILTLHNLEKAGLLKPQTGGRNNYPTIRKTLRLWMDDVNEQ
NPTDISYVYSGYAPLSVRLAQLLSRPGWRSIEEVLRILPGPHFEERQPLPTGLQKKRQPG
ENRVTLIFFLGGVTFAEIAALRFLSQLEDGGTEYVIATTKLMNGTSWIEALMEKPF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6151 Download

>pFN21AB6151  6493 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCTCATCTGTCCTACGGCCGAGTGAACCT
AAACGTGTTGCGCGAGGCGGTGCGTCGCGAGCTGCGCGAGTTCCTGGACAAGTGCGCAGG
AAGCAAGGCAATAGTTTGGGATGAATACCTAACTGGACCCTTTGGCCTGATTGCACAGTA
TTCACTATTGAAGGAACATGAAGTGGAAAAAATGTTCACACTTAAAGGAAATCGTTTGCC
GGCAGCTGATGTGAAGAATATAATTTTTTTTGTCAGACCCAGGCTAGAGTTGATGGATAT
AATCGCTGAAAACGTGCTCAGTGAAGATAGACGAGGCCCAACGAGAGATTTTCATATTCT
GTTTGTGCCACGCCGTAGCCTGTTGTGCGAACAGCGGTTGAAGGATCTGGGTGTCTTGGG
ATCCTTTATTCACAGGGAGGAGTACAGCTTAGATCTCATTCCATTCGATGGGGATCTCTT
ATCCATGGAATCAGAGGGTGCATTCAAAGAGTGCTACCTGGAGGGTGACCAGACGAGCCT
GTACCACGCAGCCAAGGGGCTGATGACCCTGCAAGCTCTGTATGGAACGATCCCCCAGAT
CTTTGGGAAAGGAGAATGCGCTCGGCAAGTGGCCAATATGATGATCAGGATGAAGAGAGA
GTTTACAGGAAGCCAGAATTCAATATTTCCTGTTTTTGATAATCTCTTGTTGCTTGATCG
GAATGTGGATTTATTAACACCTCTTGCCACTCAGCTGACATATGAAGGACTCATTGATGA
AATTTATGGCATTCAGAACAGTTATGTGAAATTACCTCCAGAGAAATTTGCACCTAAGAA
ACAGGGCGATGGTGGTAAGGACCTCCCCACGGAAGCAAAGAAGCTGCAGCTGAATTCTGC
AGAGGAGCTCTATGCTGAGATCCGAGATAAGAACTTCAACGCAGTTGGCTCTGTGCTCAG
CAAGAAAGCAAAGATCATCTCTGCAGCATTCGAGGAAAGACACAATGCTAAGACCGTGGG
GGAGATCAAGCAGTTTGTTTCCCAGTTGCCCCACATGCAGGCAGCAAGGGGCTCGCTTGC
AAACCATACCTCAATTGCAGAATTGATCAAAGATGTCACTACTTCTGAAGACTTTTTTGA
TAAATTAACCGTGGAACAGGAGTTTATGTCTGGAATAGACACTGATAAGGTCAACAATTA
CATTGAGGATTGTATCGCCCAAAAGCACTCGTTGATCAAGGTGTTAAGACTAGTTTGCCT
CCAATCCGTGTGTAATAGTGGGCTCAAACAAAAAGTTTTGGATTATTACAAAAGAGAGAT
TCTCCAGACATACGGCTATGAGCACATATTGACCTTACACAACCTGGAGAAGGCCGGCCT
GCTGAAACCGCAGACGGGGGGCAGAAACAATTACCCAACTATACGGAAAACATTACGCCT
CTGGATGGATGATGTTAATGAGCAAAACCCCACGGACATATCGTATGTGTACAGTGGGTA
TGCCCCGCTCAGTGTGCGGCTGGCCCAGCTGCTTTCCCGGCCTGGCTGGCGGAGCATCGA
GGAGGTCCTCCGCATCCTCCCAGGGCCCCACTTTGAGGAGCGGCAGCCACTGCCCACAGG
ACTGCAGAAGAAACGTCAACCGGGAGAAAACCGAGTGACTCTGATATTTTTCCTTGGGGG
CGTAACCTTCGCTGAAATTGCTGCCCTGCGATTTCTCTCCCAGTTGGAAGATGGAGGTAC
AGAATATGTCATTGCCACCACTAAACTAATGAATGGAACCAGTTGGATAGAGGCTCTGAT
GGAAAAACCTTTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT