RNF25 ORF clone FHC03430

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC03430

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Clone info.

Species

human

Clone Name

pFN21AB5855 Sequence (with vector) Restriction Map

Product ID

FHC03430

Flexi Clone PID

FXC03430

ORF Sequence

DNA sequence (1377 bp)
Protein sequence (459 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527514]

Gene

KIBB5855

Symbol

RNF25

Alias : AO7

Description

ring finger protein 25

Original Clone ID

cs00555

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1377 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 459 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2420.1 2.1e-97 100.0 RNF25 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR006575 18 123 PF05773 RWD domain HMMSmart IPR006575 18 128 SM00591 RWD domain IPR001841 135 201 SM00184 Zinc finger ProfileScan IPR006575 18 128 PS50908 RWD domain IPR001841 135 202 PS50089 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_071898 459 616014 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5855 Download

>KIBB5855  1377 bp
ATGGCGGCGTCTGCGTCTGCAGCTGCAGGGGAGGAGGACTGGGTCCTTCCCTCTGAAGTT
GAAGTATTGGAGTCCATCTATCTAGATGAACTACAGGTGATTAAAGGAAATGGCAGAACT
TCACCATGGGAGATCTACATCACTTTGCATCCTGCCACTGCAGAGGACCAGGATTCACAG
TATGTCTGCTTCACTCTGGTGCTTCAGGTCCCAGCAGAGTATCCCCATGAGGTGCCACAG
ATCTCTATCCGAAATCCCCGAGGACTTTCAGATGAACAGATCCACACGATCTTACAGGTG
CTGGGCCACGTGGCCAAGGCTGGGCTGGGCACTGCCATGCTGTATGAACTCATTGAGAAA
GGGAAGGAAATTCTCACAGATAACAACATCCCTCATGGCCAGTGTGTCATCTGCCTCTAT
GGTTTCCAGGAGAAGGAGGCCTTTACCAAAACACCCTGTTACCACTACTTCCACTGCCAC
TGCCTTGCTCGGTACATCCAGCACATGGAGCAAGAGCTGAAGGCACAAGGACAGGAGCAG
GAACAGGAACGGCAGCATGCTACAACCAAACAGAAGGCAGTCGGTGTGCAGTGTCCAGTG
TGCAGAGAGCCCCTCGTGTATGATCTTGCCTCACTGAAAGCAGCCCCTGAACCCCAACAG
CCCATGGAGCTGTACCAGCCCAGTGCAGAGAGCTTGCGCCAGCAAGAAGAACGCAAGCGG
CTCTACCAGAGGCAGCAGGAGCGGGGGGGAATCATTGACCTTGAGGCTGAGCGAAACCGA
TACTTCATCAGCCTTCAGCAGCCTCCTGCCCCTGCGGAACCTGAGTCAGCTGTAGATGTC
TCCAAAGGATCCCAACCACCCAGCACCCTTGCAGCAGAACTATCCACCTCACCAGCCGTC
CAATCCACTTTGCCACCTCCTCTGCCTGTGGCGACCCAGCACATATGTGAGAAGATTCCA
GGGACCAGGTCAAATCAGCAAAGGTTGGGCGAAACCCAGAAAGCTATGCTAGATCCCCCC
AAGCCCAGTCGAGGTCCCTGGCGACAGCCCGAACGGAGGCACCCAAAGGGAGGGGAGTGC
CACGCCCCTAAAGGTACCCGTGACACCCAGGAACTGCCACCTCCTGAGGGGCCCCTCAAG
GAGCCCATGGACCTAAAGCCAGAACCCCATAGCCAAGGAGTTGAAGGTCCTCCACAAGAG
AAGGGGCCTGGCAGCTGGCAGGGGCCCCCACCCCGCAGGACTCGGGACTGTGTTCGCTGG
GAGCGCTCTAAAGGCCGGACACCCGGTTCTTCCTACCCTCGCCTGCCTCGGGGCCAGGGA
GCATACCGGCCTGGTACTCGGAGGGAGTCCCTGGGCCTGGAATCTAAGGATGGTTCC

ORF Protein

Cloned ORF protein sequence for pFN21AB5855 Download

>KIBB5855  459 aa
MAASASAAAGEEDWVLPSEVEVLESIYLDELQVIKGNGRTSPWEIYITLHPATAEDQDSQ
YVCFTLVLQVPAEYPHEVPQISIRNPRGLSDEQIHTILQVLGHVAKAGLGTAMLYELIEK
GKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFHCHCLARYIQHMEQELKAQGQEQ
EQERQHATTKQKAVGVQCPVCREPLVYDLASLKAAPEPQQPMELYQPSAESLRQQEERKR
LYQRQQERGGIIDLEAERNRYFISLQQPPAPAEPESAVDVSKGSQPPSTLAAELSTSPAV
QSTLPPPLPVATQHICEKIPGTRSNQQRLGETQKAMLDPPKPSRGPWRQPERRHPKGGEC
HAPKGTRDTQELPPPEGPLKEPMDLKPEPHSQGVEGPPQEKGPGSWQGPPPRRTRDCVRW
ERSKGRTPGSSYPRLPRGQGAYRPGTRRESLGLESKDGS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5855 Download

>pFN21AB5855  6082 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCGTCTGCGTCTGCAGCTGCAGGGGAGGA
GGACTGGGTCCTTCCCTCTGAAGTTGAAGTATTGGAGTCCATCTATCTAGATGAACTACA
GGTGATTAAAGGAAATGGCAGAACTTCACCATGGGAGATCTACATCACTTTGCATCCTGC
CACTGCAGAGGACCAGGATTCACAGTATGTCTGCTTCACTCTGGTGCTTCAGGTCCCAGC
AGAGTATCCCCATGAGGTGCCACAGATCTCTATCCGAAATCCCCGAGGACTTTCAGATGA
ACAGATCCACACGATCTTACAGGTGCTGGGCCACGTGGCCAAGGCTGGGCTGGGCACTGC
CATGCTGTATGAACTCATTGAGAAAGGGAAGGAAATTCTCACAGATAACAACATCCCTCA
TGGCCAGTGTGTCATCTGCCTCTATGGTTTCCAGGAGAAGGAGGCCTTTACCAAAACACC
CTGTTACCACTACTTCCACTGCCACTGCCTTGCTCGGTACATCCAGCACATGGAGCAAGA
GCTGAAGGCACAAGGACAGGAGCAGGAACAGGAACGGCAGCATGCTACAACCAAACAGAA
GGCAGTCGGTGTGCAGTGTCCAGTGTGCAGAGAGCCCCTCGTGTATGATCTTGCCTCACT
GAAAGCAGCCCCTGAACCCCAACAGCCCATGGAGCTGTACCAGCCCAGTGCAGAGAGCTT
GCGCCAGCAAGAAGAACGCAAGCGGCTCTACCAGAGGCAGCAGGAGCGGGGGGGAATCAT
TGACCTTGAGGCTGAGCGAAACCGATACTTCATCAGCCTTCAGCAGCCTCCTGCCCCTGC
GGAACCTGAGTCAGCTGTAGATGTCTCCAAAGGATCCCAACCACCCAGCACCCTTGCAGC
AGAACTATCCACCTCACCAGCCGTCCAATCCACTTTGCCACCTCCTCTGCCTGTGGCGAC
CCAGCACATATGTGAGAAGATTCCAGGGACCAGGTCAAATCAGCAAAGGTTGGGCGAAAC
CCAGAAAGCTATGCTAGATCCCCCCAAGCCCAGTCGAGGTCCCTGGCGACAGCCCGAACG
GAGGCACCCAAAGGGAGGGGAGTGCCACGCCCCTAAAGGTACCCGTGACACCCAGGAACT
GCCACCTCCTGAGGGGCCCCTCAAGGAGCCCATGGACCTAAAGCCAGAACCCCATAGCCA
AGGAGTTGAAGGTCCTCCACAAGAGAAGGGGCCTGGCAGCTGGCAGGGGCCCCCACCCCG
CAGGACTCGGGACTGTGTTCGCTGGGAGCGCTCTAAAGGCCGGACACCCGGTTCTTCCTA
CCCTCGCCTGCCTCGGGGCCAGGGAGCATACCGGCCTGGTACTCGGAGGGAGTCCCTGGG
CCTGGAATCTAAGGATGGTTCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT