GPR34 ORF clone FHC03042

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC03042

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Clone info.

Species

human

Clone Name

pFN21AB8818 Sequence (with vector) Restriction Map

Product ID

FHC03042

Flexi Clone PID

FXC03042

ORF Sequence

DNA sequence (1143 bp)
Protein sequence (381 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464544]

Gene

KIBB8818

Symbol

GPR34

Alias : LYPSR1

Description

G protein-coupled receptor 34, transcript variant 1

Original Clone ID

cp00853

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1143 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 381 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14258.1 3e-124 100.0 GPR34 CCDS3156.1 3.7e-28 33.2 P2RY14 CCDS2148.1 1.3e-24 31.4 GPR17 CCDS3159.1 1.6e-24 32.6 P2RY12 CCDS9410.1 9.3e-23 31.9 LPAR6 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 56 80 PR00237 GPCR IPR000276 89 110 PR00237 GPCR IPR002286 103 114 PR01157 P2 purinoceptor IPR002286 127 144 PR01157 P2 purinoceptor IPR000276 134 156 PR00237 GPCR IPR000276 219 242 PR00237 GPCR IPR000276 264 288 PR00237 GPCR IPR002286 278 289 PR01157 P2 purinoceptor IPR000276 309 335 PR00237 GPCR HMMPfam IPR000276 71 327 PF00001 GPCR ProfileScan IPR017452 71 327 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 57 LTTSYSVIFIVGLVGNIIALYVF 79 PRIMARY 23 2 90 QIYLLNVAIADLLLIFCLPFRIM 112 PRIMARY 23 3 126 LCKVVGTLFYMNMYISIILLGFI 148 PRIMARY 23 4 171 SIYVCCIVWMLALGGFLTMIILT 193 PRIMARY 23 5 217 AIFNFILVVMFWLIFLLIILSYI 239 PRIMARY 23 6 263 TTARNSFIVLIIFTICFVPYHAF 285 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001091048 381 300241 100.0 100.0 NP_005291 381 300241 100.0 100.0 XP_005272654 381 300241 100.0 100.0 XP_011511642 338 610116 33.2 83.2 XP_005247979 338 610116 33.2 83.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8818 Download

>KIBB8818  1143 bp
ATGAGAAGTCATACCATAACAATGACGACAACTTCAGTCAGCAGCTGGCCTTACTCCTCC
CACAGAATGCGCTTTATAACCAATCATAGCGACCAACCGCCACAAAACTTCTCAGCAACA
CCAAATGTTACTACCTGTCCCATGGATGAAAAATTGCTATCTACTGTGTTAACCACATCC
TACTCTGTTATTTTCATCGTGGGACTGGTTGGGAACATAATCGCCCTCTATGTATTTCTG
GGTATTCACCGTAAAAGAAATTCCATTCAAATTTATCTACTTAACGTAGCCATTGCAGAC
CTCCTACTCATCTTCTGCCTCCCTTTCCGAATAATGTATCATATTAACCAAAACAAGTGG
ACACTAGGTGTGATTCTGTGCAAGGTTGTGGGAACACTGTTTTATATGAACATGTACATT
AGCATTATTTTGCTTGGATTCATCAGTTTGGATCGCTATATAAAAATTAATCGGTCTATA
CAGCAACGGAAGGCAATAACAACCAAACAAAGTATTTATGTCTGTTGTATAGTATGGATG
CTTGCTCTTGGTGGATTCCTAACTATGATTATTTTAACACTTAAGAAAGGAGGGCATAAT
TCCACAATGTGTTTCCATTACAGAGATAAGCATAACGCAAAAGGAGAAGCCATTTTTAAC
TTCATTCTTGTGGTAATGTTCTGGCTAATTTTCTTACTAATAATCCTTTCATATATTAAG
ATTGGGAAGAATCTATTGAGGATTTCTAAAAGGAGGTCAAAATTTCCTAATTCTGGTAAA
TATGCCACTACAGCTCGTAACTCCTTTATTGTACTTATCATTTTTACTATATGTTTTGTT
CCCTATCATGCCTTTCGATTCATCTACATTTCTTCACAGCTAAATGTATCATCTTGCTAC
TGGAAAGAAATTGTTCACAAAACCAATGAGATCATGCTGGTTCTCTCATCTTTCAATAGT
TGCTTAGATCCAGTCATGTATTTCCTGATGTCCAGTAACATTCGCAAAATAATGTGCCAA
CTTCTTTTTAGACGATTTCAAGGTGAACCAAGTAGGAGTGAAAGCACTTCAGAATTTAAA
CCAGGATACTCCCTGCATGATACATCTGTGGCAGTGAAAATACAGTCTAGTTCTAAAAGT
ACT

ORF Protein

Cloned ORF protein sequence for pFN21AB8818 Download

>KIBB8818  381 aa
MRSHTITMTTTSVSSWPYSSHRMRFITNHSDQPPQNFSATPNVTTCPMDEKLLSTVLTTS
YSVIFIVGLVGNIIALYVFLGIHRKRNSIQIYLLNVAIADLLLIFCLPFRIMYHINQNKW
TLGVILCKVVGTLFYMNMYISIILLGFISLDRYIKINRSIQQRKAITTKQSIYVCCIVWM
LALGGFLTMIILTLKKGGHNSTMCFHYRDKHNAKGEAIFNFILVVMFWLIFLLIILSYIK
IGKNLLRISKRRSKFPNSGKYATTARNSFIVLIIFTICFVPYHAFRFIYISSQLNVSSCY
WKEIVHKTNEIMLVLSSFNSCLDPVMYFLMSSNIRKIMCQLLFRRFQGEPSRSESTSEFK
PGYSLHDTSVAVKIQSSSKST

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8818 Download

>pFN21AB8818  5848 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGAAGTCATACCATAACAATGACGACAACTTC
AGTCAGCAGCTGGCCTTACTCCTCCCACAGAATGCGCTTTATAACCAATCATAGCGACCA
ACCGCCACAAAACTTCTCAGCAACACCAAATGTTACTACCTGTCCCATGGATGAAAAATT
GCTATCTACTGTGTTAACCACATCCTACTCTGTTATTTTCATCGTGGGACTGGTTGGGAA
CATAATCGCCCTCTATGTATTTCTGGGTATTCACCGTAAAAGAAATTCCATTCAAATTTA
TCTACTTAACGTAGCCATTGCAGACCTCCTACTCATCTTCTGCCTCCCTTTCCGAATAAT
GTATCATATTAACCAAAACAAGTGGACACTAGGTGTGATTCTGTGCAAGGTTGTGGGAAC
ACTGTTTTATATGAACATGTACATTAGCATTATTTTGCTTGGATTCATCAGTTTGGATCG
CTATATAAAAATTAATCGGTCTATACAGCAACGGAAGGCAATAACAACCAAACAAAGTAT
TTATGTCTGTTGTATAGTATGGATGCTTGCTCTTGGTGGATTCCTAACTATGATTATTTT
AACACTTAAGAAAGGAGGGCATAATTCCACAATGTGTTTCCATTACAGAGATAAGCATAA
CGCAAAAGGAGAAGCCATTTTTAACTTCATTCTTGTGGTAATGTTCTGGCTAATTTTCTT
ACTAATAATCCTTTCATATATTAAGATTGGGAAGAATCTATTGAGGATTTCTAAAAGGAG
GTCAAAATTTCCTAATTCTGGTAAATATGCCACTACAGCTCGTAACTCCTTTATTGTACT
TATCATTTTTACTATATGTTTTGTTCCCTATCATGCCTTTCGATTCATCTACATTTCTTC
ACAGCTAAATGTATCATCTTGCTACTGGAAAGAAATTGTTCACAAAACCAATGAGATCAT
GCTGGTTCTCTCATCTTTCAATAGTTGCTTAGATCCAGTCATGTATTTCCTGATGTCCAG
TAACATTCGCAAAATAATGTGCCAACTTCTTTTTAGACGATTTCAAGGTGAACCAAGTAG
GAGTGAAAGCACTTCAGAATTTAAACCAGGATACTCCCTGCATGATACATCTGTGGCAGT
GAAAATACAGTCTAGTTCTAAAAGTACTGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT