RHBDD3 ORF clone FHC02958

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC02958

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Clone info.

Species

human

Clone Name

pFN21AB8715 Sequence (with vector) Restriction Map

Product ID

FHC02958

Flexi Clone PID

FXC02958

ORF Sequence

DNA sequence (1158 bp)
Protein sequence (386 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463229]

Gene

KIBB8715

Symbol

RHBDD3

Alias : C22orf3, HS984G1A, PTAG

Description

rhomboid domain containing 3

Original Clone ID

oc00378

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1158 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 386 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13850.1 3.2e-117 100.0 RHBDD3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR022764 46 185 PF01694 Peptidase S54
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 17 ASSVLMLLMSTLWLVGAGPGLVL 39 PRIMARY 23 2 59 GHTALPGLLLSLLLLPTVGWQQ 80 PRIMARY 22 3 91 HASALLALASGLLAVLLAGLGLS 113 PRIMARY 23 4 164 PFLQLLCGLLAGLAYAAGAFRWL 186 PRIMARY 23 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8715 Download

>KIBB8715  1158 bp
ATGCATGCCAGGGGCCCCCATGGCCAACTGTCCCCAGCACTGCCTCTGGCCTCCTCAGTC
CTGATGCTGCTGATGAGCACCCTGTGGCTGGTGGGGGCCGGCCCCGGCCTGGTCCTGGCC
CCGGAGCTGTTGCTGGACCCCTGGCAGGTGCACCGGCTGCTGACCCATGCCCTGGGCCAC
ACGGCCCTGCCAGGCCTGCTCCTGAGCCTGCTGCTCCTGCCCACTGTGGGCTGGCAGCAG
GAGTGCCACCTGGGCACGCTGAGATTCCTGCATGCCTCAGCCCTGCTCGCCCTGGCTTCT
GGGCTGCTGGCAGTGCTGCTGGCAGGCCTTGGGCTGTCCAGTGCAGCCGGCAGCTGTGGA
TACATGCCTGTCCACCTGGCCATGCTGGCTGGGGAGGGACACCGCCCTAGACGGCCCCGT
GGGGCACTGCCACCGTGGCTGTCGCCGTGGCTGCTGCTTGCCCTGACCCCACTGCTCAGC
TCTGAGCCACCCTTCCTGCAGCTCCTTTGCGGCCTCCTTGCCGGCCTGGCCTATGCAGCT
GGGGCCTTCCGGTGGCTGGAACCCTCAGAGCGACGGCTGCAGGTGCTGCAGGAGGGCGTC
TTGTGCAGGACCTTGGCGGGGTGCTGGCCCCTGAGGCTCCTTGCCACCCCGGGTAGCCTG
GCGGAGCTGCCTGTCACCCATCCTGCCGGAGTGAGGCCTCCCATCCCTGGACCGCCTTAT
GTGGCCTCCCCTGACCTCTGGTCCCACTGGGAAGACTCAGCCCTGCCCCCACCAAGCCTG
AGGCCTGTGCAGCCCACCTGGGAGGGCTCCTCAGAGGCAGGCCTGGACTGGGCTGGGGCC
AGCTTCTCCCCAGGGACTCCGATGTGGGCGGCCTTGGATGAGCAGATGCTGCAGGAGGGC
ATCCAGGCCTCGCTTCTTGACGGGCCAGCCCAGGAACCCCAGAGCGCACCATGGCTGTCC
AAGTCCTCTGTCTCCTCTCTGCGGCTGCAGCAGCTGGAGCGCATGGGCTTCCCTACGGAG
CAGGCGGTGGTGGCACTGGCAGCCACAGGCCGTGTGGAGGGTGCCGTGTCACTGTTGGTT
GGAGGACAAGTGGGCACTGAGACCCTGGTGACCCATGGAAAGGGTGGGCCTGCCCACTCC
GAGGGTCCTGGGCCTCCC

ORF Protein

Cloned ORF protein sequence for pFN21AB8715 Download

>KIBB8715  386 aa
MHARGPHGQLSPALPLASSVLMLLMSTLWLVGAGPGLVLAPELLLDPWQVHRLLTHALGH
TALPGLLLSLLLLPTVGWQQECHLGTLRFLHASALLALASGLLAVLLAGLGLSSAAGSCG
YMPVHLAMLAGEGHRPRRPRGALPPWLSPWLLLALTPLLSSEPPFLQLLCGLLAGLAYAA
GAFRWLEPSERRLQVLQEGVLCRTLAGCWPLRLLATPGSLAELPVTHPAGVRPPIPGPPY
VASPDLWSHWEDSALPPPSLRPVQPTWEGSSEAGLDWAGASFSPGTPMWAALDEQMLQEG
IQASLLDGPAQEPQSAPWLSKSSVSSLRLQQLERMGFPTEQAVVALAATGRVEGAVSLLV
GGQVGTETLVTHGKGGPAHSEGPGPP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8715 Download

>pFN21AB8715  5863 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCATGCCAGGGGCCCCCATGGCCAACTGTCCCC
AGCACTGCCTCTGGCCTCCTCAGTCCTGATGCTGCTGATGAGCACCCTGTGGCTGGTGGG
GGCCGGCCCCGGCCTGGTCCTGGCCCCGGAGCTGTTGCTGGACCCCTGGCAGGTGCACCG
GCTGCTGACCCATGCCCTGGGCCACACGGCCCTGCCAGGCCTGCTCCTGAGCCTGCTGCT
CCTGCCCACTGTGGGCTGGCAGCAGGAGTGCCACCTGGGCACGCTGAGATTCCTGCATGC
CTCAGCCCTGCTCGCCCTGGCTTCTGGGCTGCTGGCAGTGCTGCTGGCAGGCCTTGGGCT
GTCCAGTGCAGCCGGCAGCTGTGGATACATGCCTGTCCACCTGGCCATGCTGGCTGGGGA
GGGACACCGCCCTAGACGGCCCCGTGGGGCACTGCCACCGTGGCTGTCGCCGTGGCTGCT
GCTTGCCCTGACCCCACTGCTCAGCTCTGAGCCACCCTTCCTGCAGCTCCTTTGCGGCCT
CCTTGCCGGCCTGGCCTATGCAGCTGGGGCCTTCCGGTGGCTGGAACCCTCAGAGCGACG
GCTGCAGGTGCTGCAGGAGGGCGTCTTGTGCAGGACCTTGGCGGGGTGCTGGCCCCTGAG
GCTCCTTGCCACCCCGGGTAGCCTGGCGGAGCTGCCTGTCACCCATCCTGCCGGAGTGAG
GCCTCCCATCCCTGGACCGCCTTATGTGGCCTCCCCTGACCTCTGGTCCCACTGGGAAGA
CTCAGCCCTGCCCCCACCAAGCCTGAGGCCTGTGCAGCCCACCTGGGAGGGCTCCTCAGA
GGCAGGCCTGGACTGGGCTGGGGCCAGCTTCTCCCCAGGGACTCCGATGTGGGCGGCCTT
GGATGAGCAGATGCTGCAGGAGGGCATCCAGGCCTCGCTTCTTGACGGGCCAGCCCAGGA
ACCCCAGAGCGCACCATGGCTGTCCAAGTCCTCTGTCTCCTCTCTGCGGCTGCAGCAGCT
GGAGCGCATGGGCTTCCCTACGGAGCAGGCGGTGGTGGCACTGGCAGCCACAGGCCGTGT
GGAGGGTGCCGTGTCACTGTTGGTTGGAGGACAAGTGGGCACTGAGACCCTGGTGACCCA
TGGAAAGGGTGGGCCTGCCCACTCCGAGGGTCCTGGGCCTCCCGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT