BLMH ORF clone FHC02597

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC02597

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Clone info.

Species

human

Clone Name

pFN21AB8125 Sequence (with vector) Restriction Map

Product ID

FHC02597

Flexi Clone PID

FXC02597

ORF Sequence

DNA sequence (1365 bp)
Protein sequence (455 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463443]

Gene

KIBB8125

Symbol

BLMH

Alias : BH, BMH

Description

bleomycin hydrolase

Original Clone ID

cp00485

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1365 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 455 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS32604.1 1.6e-211 100.0 BLMH The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004134 6 451 PF03051 Peptidase C1B
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_000377 455 104300,602403 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8125 Download

>KIBB8125  1365 bp
ATGAGCAGCTCGGGACTGAATTCGGAGAAGGTAGCTGCTCTGATACAGAAACTGAATTCC
GACCCCCAGTTCGTACTTGCCCAGAATGTCGGGACCACCCACGACCTGCTGGACATCTGT
CTGAAGCGGGCCACGGTGCAGCGCGCGCAGCATGTGTTCCAGCACGCCGTGCCCCAGGAG
GGCAAGCCAATCACCAACCAGAAGAGCTCAGGGCGATGCTGGATCTTTTCTTGTCTGAAT
GTTATGAGGCTTCCATTCATGAAAAAGTTAAATATTGAAGAATTTGAGTTTAGCCAATCT
TACCTGTTTTTTTGGGACAAGGTTGAACGCTGTTATTTCTTCTTGAGTGCTTTTGTGGAC
ACAGCCCAGAGAAAGGAGCCTGAGGATGGGAGGCTGGTGCAGTTTTTGCTTATGAACCCT
GCAAATGATGGTGGCCAATGGGATATGCTTGTTAATATTGTTGAAAAATATGGTGTTATC
CCTAAGAAATGCTTCCCTGAATCTTATACAACAGAGGCAACCAGAAGGATGAATGATATT
CTGAATCACAAGATGAGAGAATTCTGTATACGACTGCGGAACCTGGTACACAGTGGAGCA
ACCAAAGGAGAAATCTCGGCCACACAGGACGTCATGATGGAGGAGATATTCCGAGTGGTG
TGCATCTGTTTGGGTAATCCACCAGAGACATTCACCTGGGAATATCGAGACAAAGATAAA
AATTATCAGAAAATTGGCCCCATAACACCCTTGGAGTTTTACAGGGAACATGTCAAGCCA
CTCTTCAATATGGAAGATAAGATTTGTTTAGTGAATGACCCTAGGCCCCAGCACAAGTAC
AACAAACTTTACACAGTGGAATACTTAAGCAATATGGTTGGAGGGAGAAAAACTCTATAC
AACAACCAGCCCATTGACTTCCTGAAAAAGATGGTTGCTGCCTCCATCAAAGATGGAGAG
GCTGTGTGGTTTGGCTGTGATGTTGGAAAACACTTCAATAGCAAGCTGGGCCTCAGTGAC
ATGAATCTCTATGACCATGAGTTAGTGTTTGGTGTCTCCTTGAAGAACATGAATAAAGCG
GAGAGGCTGACTTTTGGTGAGTCACTTATGACCCACGCCATGACCTTCACTGCTGTCTCA
GAGAAGGATGATCAGGATGGTGCTTTCACAAAATGGAGAGTGGAGAATTCATGGGGTGAA
GACCATGGCCACAAAGGTTACCTGTGCATGACAGATGAGTGGTTCTCTGAGTATGTCTAC
GAAGTGGTGGTGGACAGGAAGCATGTCCCTGAAGAGGTGCTAGCTGTGTTAGAGCAGGAA
CCCATTATCCTGCCAGCATGGGACCCCATGGGAGCTTTGGCTGAG

ORF Protein

Cloned ORF protein sequence for pFN21AB8125 Download

>KIBB8125  455 aa
MSSSGLNSEKVAALIQKLNSDPQFVLAQNVGTTHDLLDICLKRATVQRAQHVFQHAVPQE
GKPITNQKSSGRCWIFSCLNVMRLPFMKKLNIEEFEFSQSYLFFWDKVERCYFFLSAFVD
TAQRKEPEDGRLVQFLLMNPANDGGQWDMLVNIVEKYGVIPKKCFPESYTTEATRRMNDI
LNHKMREFCIRLRNLVHSGATKGEISATQDVMMEEIFRVVCICLGNPPETFTWEYRDKDK
NYQKIGPITPLEFYREHVKPLFNMEDKICLVNDPRPQHKYNKLYTVEYLSNMVGGRKTLY
NNQPIDFLKKMVAASIKDGEAVWFGCDVGKHFNSKLGLSDMNLYDHELVFGVSLKNMNKA
ERLTFGESLMTHAMTFTAVSEKDDQDGAFTKWRVENSWGEDHGHKGYLCMTDEWFSEYVY
EVVVDRKHVPEEVLAVLEQEPIILPAWDPMGALAE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8125 Download

>pFN21AB8125  6070 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGCAGCTCGGGACTGAATTCGGAGAAGGTAGC
TGCTCTGATACAGAAACTGAATTCCGACCCCCAGTTCGTACTTGCCCAGAATGTCGGGAC
CACCCACGACCTGCTGGACATCTGTCTGAAGCGGGCCACGGTGCAGCGCGCGCAGCATGT
GTTCCAGCACGCCGTGCCCCAGGAGGGCAAGCCAATCACCAACCAGAAGAGCTCAGGGCG
ATGCTGGATCTTTTCTTGTCTGAATGTTATGAGGCTTCCATTCATGAAAAAGTTAAATAT
TGAAGAATTTGAGTTTAGCCAATCTTACCTGTTTTTTTGGGACAAGGTTGAACGCTGTTA
TTTCTTCTTGAGTGCTTTTGTGGACACAGCCCAGAGAAAGGAGCCTGAGGATGGGAGGCT
GGTGCAGTTTTTGCTTATGAACCCTGCAAATGATGGTGGCCAATGGGATATGCTTGTTAA
TATTGTTGAAAAATATGGTGTTATCCCTAAGAAATGCTTCCCTGAATCTTATACAACAGA
GGCAACCAGAAGGATGAATGATATTCTGAATCACAAGATGAGAGAATTCTGTATACGACT
GCGGAACCTGGTACACAGTGGAGCAACCAAAGGAGAAATCTCGGCCACACAGGACGTCAT
GATGGAGGAGATATTCCGAGTGGTGTGCATCTGTTTGGGTAATCCACCAGAGACATTCAC
CTGGGAATATCGAGACAAAGATAAAAATTATCAGAAAATTGGCCCCATAACACCCTTGGA
GTTTTACAGGGAACATGTCAAGCCACTCTTCAATATGGAAGATAAGATTTGTTTAGTGAA
TGACCCTAGGCCCCAGCACAAGTACAACAAACTTTACACAGTGGAATACTTAAGCAATAT
GGTTGGAGGGAGAAAAACTCTATACAACAACCAGCCCATTGACTTCCTGAAAAAGATGGT
TGCTGCCTCCATCAAAGATGGAGAGGCTGTGTGGTTTGGCTGTGATGTTGGAAAACACTT
CAATAGCAAGCTGGGCCTCAGTGACATGAATCTCTATGACCATGAGTTAGTGTTTGGTGT
CTCCTTGAAGAACATGAATAAAGCGGAGAGGCTGACTTTTGGTGAGTCACTTATGACCCA
CGCCATGACCTTCACTGCTGTCTCAGAGAAGGATGATCAGGATGGTGCTTTCACAAAATG
GAGAGTGGAGAATTCATGGGGTGAAGACCATGGCCACAAAGGTTACCTGTGCATGACAGA
TGAGTGGTTCTCTGAGTATGTCTACGAAGTGGTGGTGGACAGGAAGCATGTCCCTGAAGA
GGTGCTAGCTGTGTTAGAGCAGGAACCCATTATCCTGCCAGCATGGGACCCCATGGGAGC
TTTGGCTGAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT