PDLIM1 ORF clone FHC02548

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC02548

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Clone info.

Species

human

Clone Name

pFN21AB7649 Sequence (with vector) Restriction Map

Product ID

FHC02548

Flexi Clone PID

FXC02548

ORF Sequence

DNA sequence (987 bp)
Protein sequence (329 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464004]

Gene

KIBB7649

Symbol

PDLIM1

Alias : CLIM1, CLP-36, CLP36, HEL-S-112, hCLIM1

Description

PDZ and LIM domain 1

Original Clone ID

cs00149

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 987 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 329 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7441.1 4.9e-86 100.0 PDLIM1 CCDS4152.1 8.3e-33 44.9 PDLIM4 CCDS47172.1 4.1e-20 53.4 PDLIM3 CCDS75218.1 1.2e-19 48.7 PDLIM3 CCDS3844.1 1.2e-19 44.5 PDLIM3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001478 5 81 PF00595 PDZ/DHR/GLGF IPR001781 260 310 PF00412 Zinc finger HMMSmart IPR001478 12 85 SM00228 PDZ/DHR/GLGF IPR006643 138 163 SM00735 ZASP IPR001781 259 310 SM00132 Zinc finger ProfileScan IPR001478 3 85 PS50106 PDZ/DHR/GLGF IPR001781 258 317 PS50023 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_066272 329 605900 100.0 100.0 NP_003678 330 166710,603422 44.9 98.8 NP_001107579 316 605889 53.4 99.1 NP_055291 364 605889 44.5 99.1 NP_001244891 276 605889 48.2 99.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7649 Download

>KIBB7649  987 bp
ATGACCACCCAGCAGATAGACCTCCAGGGCCCGGGGCCGTGGGGCTTCCGCCTCGTGGGC
GGCAAGGACTTCGAGCAGCCTCTCGCCATTTCCCGGGTCACTCCTGGAAGCAAGGCGGCT
CTAGCTAATTTATGTATTGGAGATGTAATCACAGCCATTGATGGGGAAAATACTAGCAAT
ATGACACACTTGGAAGCTCAGAACAGAATCAAAGGCTGCACAGACAACTTGACTCTCACT
GTAGCCAGATCTGAACATAAAGTCTGGTCTCCTCTGGTGACGGAGGAAGGGAAGCGTCAT
CCATACAAGATGAATTTAGCCTCTGAACCCCAGGAGGTCCTGCACATAGGAAGCGCCCAC
AACCGAAGTGCCATGCCCTTTACCGCCTCGCCTGCCTCCAGCACTACTGCCAGGGTCATC
ACAAACCAGTACAACAACCCAGCTGGCCTCTACTCTTCTGAAAATATCTCCAACTTCAAC
AATGCCCTGGAGTCAAAGACTGCTGCCAGCGGGGTGGAGGCGAACAGCAGACCCTTAGAC
CATGCTCAGCCTCCAAGCAGCCTTGTCATCGACAAAGAATCTGAAGTTTACAAGATGCTT
CAGGAGAAACAGGAGTTGAATGAGCCCCCGAAACAGTCCACGTCTTTCTTGGTTTTGCAG
GAAATCCTGGAGTCTGAAGAAAAAGGGGATCCCAACAAGCCCTCAGGATTCAGAAGTGTT
AAAGCTCCTGTCACTAAAGTGGCTGCGTCGATTGGAAATGCTCAGAAGTTGCCTATGTGT
GACAAATGTGGCACTGGGATTGTTGGTGTGTTTGTGAAGCTGCGGGACCGTCACCGCCAC
CCTGAGTGTTATGTGTGCACTGACTGTGGCACCAACCTGAAACAGAAGGGCCATTTCTTT
GTGGAGGATCAAATCTACTGTGAGAAGCATGCCCGGGAGCGAGTCACACCACCTGAGGGT
TATGAAGTGGTCACTGTGTTCCCCAAG

ORF Protein

Cloned ORF protein sequence for pFN21AB7649 Download

>KIBB7649  329 aa
MTTQQIDLQGPGPWGFRLVGGKDFEQPLAISRVTPGSKAALANLCIGDVITAIDGENTSN
MTHLEAQNRIKGCTDNLTLTVARSEHKVWSPLVTEEGKRHPYKMNLASEPQEVLHIGSAH
NRSAMPFTASPASSTTARVITNQYNNPAGLYSSENISNFNNALESKTAASGVEANSRPLD
HAQPPSSLVIDKESEVYKMLQEKQELNEPPKQSTSFLVLQEILESEEKGDPNKPSGFRSV
KAPVTKVAASIGNAQKLPMCDKCGTGIVGVFVKLRDRHRHPECYVCTDCGTNLKQKGHFF
VEDQIYCEKHARERVTPPEGYEVVTVFPK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7649 Download

>pFN21AB7649  5692 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACCACCCAGCAGATAGACCTCCAGGGCCCGGG
GCCGTGGGGCTTCCGCCTCGTGGGCGGCAAGGACTTCGAGCAGCCTCTCGCCATTTCCCG
GGTCACTCCTGGAAGCAAGGCGGCTCTAGCTAATTTATGTATTGGAGATGTAATCACAGC
CATTGATGGGGAAAATACTAGCAATATGACACACTTGGAAGCTCAGAACAGAATCAAAGG
CTGCACAGACAACTTGACTCTCACTGTAGCCAGATCTGAACATAAAGTCTGGTCTCCTCT
GGTGACGGAGGAAGGGAAGCGTCATCCATACAAGATGAATTTAGCCTCTGAACCCCAGGA
GGTCCTGCACATAGGAAGCGCCCACAACCGAAGTGCCATGCCCTTTACCGCCTCGCCTGC
CTCCAGCACTACTGCCAGGGTCATCACAAACCAGTACAACAACCCAGCTGGCCTCTACTC
TTCTGAAAATATCTCCAACTTCAACAATGCCCTGGAGTCAAAGACTGCTGCCAGCGGGGT
GGAGGCGAACAGCAGACCCTTAGACCATGCTCAGCCTCCAAGCAGCCTTGTCATCGACAA
AGAATCTGAAGTTTACAAGATGCTTCAGGAGAAACAGGAGTTGAATGAGCCCCCGAAACA
GTCCACGTCTTTCTTGGTTTTGCAGGAAATCCTGGAGTCTGAAGAAAAAGGGGATCCCAA
CAAGCCCTCAGGATTCAGAAGTGTTAAAGCTCCTGTCACTAAAGTGGCTGCGTCGATTGG
AAATGCTCAGAAGTTGCCTATGTGTGACAAATGTGGCACTGGGATTGTTGGTGTGTTTGT
GAAGCTGCGGGACCGTCACCGCCACCCTGAGTGTTATGTGTGCACTGACTGTGGCACCAA
CCTGAAACAGAAGGGCCATTTCTTTGTGGAGGATCAAATCTACTGTGAGAAGCATGCCCG
GGAGCGAGTCACACCACCTGAGGGTTATGAAGTGGTCACTGTGTTCCCCAAGGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT