MAGEB3 ORF clone FHC02282

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Halotag ORF Clone FHC02282

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Clone info.

Species

human

Clone Name

pFN21AB7146 Sequence (with vector) Restriction Map

Product ID

FHC02282

Flexi Clone PID

FXC02282

ORF Sequence

DNA sequence (1038 bp)
Protein sequence (346 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463875]

Gene

KIBB7146

Symbol

MAGEB3

Alias : CT3.5

Description

melanoma antigen family B3

Original Clone ID

cp01151

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1038 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 346 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14220.1 1.1e-135 99.4 MAGEB3 CCDS14221.1 7.9e-67 55.7 MAGEB4 CCDS14222.1 2.2e-62 51.0 MAGEB1 CCDS35221.1 2.8e-59 47.6 MAGEB10 CCDS14216.1 7.6e-59 50.1 MAGEB18 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR021072 3 97 PF12440 Melanoma associated antigen IPR002190 118 288 PF01454 MAGE protein ProfileScan IPR002190 111 310 PS50838 MAGE protein
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011543816 346 300152 99.4 100.0 XP_011543815 346 300152 99.4 100.0 NP_002356 346 300152 99.4 100.0 NP_002358 346 300153 55.7 99.7 NP_796379 347 300097 51.0 99.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7146 Download

>KIBB7146  1038 bp
ATGCCTCGGGGTCAGAAGAGTACGCTCCATGCACGTGAGAAACGCCAGCAGACCCGGGGT
CAGACCCAGGATCACCAGGGTGCTCAGATCACTGCAACTAACAAGAAAAAAGTATCCTTT
TCATCCCCTCTTATTTTGGGGGCTACTATCCAGAAAAAGTCTGCTGGTAGGTCACGTAGT
GCTCTCAAGAAGCCTCAGAGAGCACTATCCACCACTACATCTGTAGATGTTTCTTACAAA
AAGTCATACAAGGGAGCCAACAGCAAAATTGAGAAAAAGCAAAGCTTCTCTCAGGGTCTA
TCCTCCACTGTGCAGTCTCACACAGACCCTCTAACCATGAAGACAAATATGTTGGTGCAG
TTCCTGATGGAAATGTACAAGATGAAAAAGCCCATTATGAAAGCAGATATGCTAAAAATT
GTCCAAAAAAGCCATAAGAATTGCTTCCCTGAGATCCTTAAAAAAGCTTCTTTCAACATG
GAGGTGGTGTTTGGTGTTGATTTAAAGAAAGTTGATTCTACCAAGGACTCCTATGTCCTT
GTCAGCAAAATGGATCTCCCCAACAATGGGACAGTGACTCGTGGGAGGGGATTTCCCAAG
ACAGGTCTCCTGCTGAATCTCCTGGGCGTGATCTTCATGAAGGGCAACTGTGCCACTGAG
GAGAAGATCTGGGAATTCCTGAATAAGATGAGAATATATGATGGGAAGAAACACTTCATA
TTTGGGGAGCCCAGAAAGCTCATCACCCAAGATTTGGTGAAGCTTAAATACCTGGAGTAC
CGACAAGTGCCCAACAGTAATCCTGCACGCTATGAATTCCTGTGGGGTCCAAGAGCCCAT
GCTGAAACCAGCAAGATGAAGGTCCTGGAGTTTTGGGCCAAGGTCAATAAAACTGTCCCC
AGTGCGTTCCAGTTCTGGTATGAAGAGGCTTTGAGAGATGAGGAAGAAAGAGTCCAAGCT
GCAGCTATGCTCAATGATGGCAGTAGTGCCATGGGCAGAAAGTGTTCCAAGGCCAAGGCT
AGCAGCTCTTCCCACGCC

ORF Protein

Cloned ORF protein sequence for pFN21AB7146 Download

>KIBB7146  346 aa
MPRGQKSTLHAREKRQQTRGQTQDHQGAQITATNKKKVSFSSPLILGATIQKKSAGRSRS
ALKKPQRALSTTTSVDVSYKKSYKGANSKIEKKQSFSQGLSSTVQSHTDPLTMKTNMLVQ
FLMEMYKMKKPIMKADMLKIVQKSHKNCFPEILKKASFNMEVVFGVDLKKVDSTKDSYVL
VSKMDLPNNGTVTRGRGFPKTGLLLNLLGVIFMKGNCATEEKIWEFLNKMRIYDGKKHFI
FGEPRKLITQDLVKLKYLEYRQVPNSNPARYEFLWGPRAHAETSKMKVLEFWAKVNKTVP
SAFQFWYEEALRDEEERVQAAAMLNDGSSAMGRKCSKAKASSSSHA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7146 Download

>pFN21AB7146  5743 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCTCGGGGTCAGAAGAGTACGCTCCATGCACG
TGAGAAACGCCAGCAGACCCGGGGTCAGACCCAGGATCACCAGGGTGCTCAGATCACTGC
AACTAACAAGAAAAAAGTATCCTTTTCATCCCCTCTTATTTTGGGGGCTACTATCCAGAA
AAAGTCTGCTGGTAGGTCACGTAGTGCTCTCAAGAAGCCTCAGAGAGCACTATCCACCAC
TACATCTGTAGATGTTTCTTACAAAAAGTCATACAAGGGAGCCAACAGCAAAATTGAGAA
AAAGCAAAGCTTCTCTCAGGGTCTATCCTCCACTGTGCAGTCTCACACAGACCCTCTAAC
CATGAAGACAAATATGTTGGTGCAGTTCCTGATGGAAATGTACAAGATGAAAAAGCCCAT
TATGAAAGCAGATATGCTAAAAATTGTCCAAAAAAGCCATAAGAATTGCTTCCCTGAGAT
CCTTAAAAAAGCTTCTTTCAACATGGAGGTGGTGTTTGGTGTTGATTTAAAGAAAGTTGA
TTCTACCAAGGACTCCTATGTCCTTGTCAGCAAAATGGATCTCCCCAACAATGGGACAGT
GACTCGTGGGAGGGGATTTCCCAAGACAGGTCTCCTGCTGAATCTCCTGGGCGTGATCTT
CATGAAGGGCAACTGTGCCACTGAGGAGAAGATCTGGGAATTCCTGAATAAGATGAGAAT
ATATGATGGGAAGAAACACTTCATATTTGGGGAGCCCAGAAAGCTCATCACCCAAGATTT
GGTGAAGCTTAAATACCTGGAGTACCGACAAGTGCCCAACAGTAATCCTGCACGCTATGA
ATTCCTGTGGGGTCCAAGAGCCCATGCTGAAACCAGCAAGATGAAGGTCCTGGAGTTTTG
GGCCAAGGTCAATAAAACTGTCCCCAGTGCGTTCCAGTTCTGGTATGAAGAGGCTTTGAG
AGATGAGGAAGAAAGAGTCCAAGCTGCAGCTATGCTCAATGATGGCAGTAGTGCCATGGG
CAGAAAGTGTTCCAAGGCCAAGGCTAGCAGCTCTTCCCACGCCGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT