PLEKHF1 ORF clone FHC02260

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC02260

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Clone info.

Species

human

Clone Name

pFN21AB7115 Sequence (with vector) Restriction Map

Product ID

FHC02260

Flexi Clone PID

FXC02260

ORF Sequence

DNA sequence (837 bp)
Protein sequence (279 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464028]

Gene

KIBB7115

Symbol

PLEKHF1

Alias : APPD, LAPF, PHAFIN1, ZFYVE15

Description

pleckstrin homology domain containing, family F (with FYVE domain) member 1

Original Clone ID

cp01120

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 837 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 279 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12417.1 3.1e-96 100.0 PLEKHF1 CCDS6267.1 1.1e-47 56.0 PLEKHF2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001849 39 130 PF00169 Pleckstrin homology domain IPR000306 148 211 PF01363 Zinc finger HMMSmart IPR001849 36 133 SM00233 Pleckstrin homology domain IPR000306 144 213 SM00064 Zinc finger ProfileScan IPR001849 35 131 PS50003 Pleckstrin homology domain IPR017455 152 212 PS50178 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_077286 279 615200 100.0 100.0 XP_011525611 279 615200 100.0 100.0 XP_005259313 364 615200 100.0 100.0 NP_078889 249 615208 56.0 90.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7115 Download

>KIBB7115  837 bp
ATGGTGGACCACTTGGCCAACACGGAGATCAACAGCCAGCGCATCGCGGCAGTGGAGAGC
TGCTTCGGGGCCTCGGGGCAGCCGCTGGCGCTGCCAGGCCGAGTGCTGCTGGGCGAGGGC
GTGCTGACCAAAGAGTGCCGCAAGAAGGCCAAGCCGCGCATCTTCTTCCTCTTTAACGAC
ATCCTGGTGTATGGCAGCATCGTGCTCAACAAGCGCAAGTACCGCAGCCAGCACATCATC
CCCCTGGAGGAGGTCACACTGGAGCTGTTGCCGGAGACGCTGCAGGCCAAGAACCGCTGG
ATGATCAAGACGGCCAAGAAGTCCTTTGTGGTGTCGGCCGCCTCCGCTACGGAGCGCCAG
GAATGGATTAGCCACATCGAGGAGTGCGTGCGGCGGCAACTGAGGGCCACGGGCCGCCCG
CCCAGCACGGAGCACGCGGCACCCTGGATCCCCGACAAGGCCACGGACATCTGCATGCGC
TGCACGCAGACGCGCTTCTCTGCCCTCACGAGGCGCCACCACTGCCGCAAGTGCGGCTTC
GTGGTCTGCGCTGAGTGCTCGCGCCAGCGCTTCCTGCTCCCGCGCCTGTCCCCCAAGCCC
GTGCGCGTCTGCAGCCTCTGCTACCGCGAACTGGCCGCCCAGCAGCGGCAGGAGGAGGCG
GAGGAGCAGGGCGCGGGGTCCCCAGGGCAGCCAGCCCACCTGGCCCGGCCCATCTGCGGA
GCGTCCAGTGGAGATGACGATGACTCCGACGAGGACAAGGAGGGCAGCAGGGACGGCGAC
TGGCCCAGCAGCGTGGAGTTCTACGCCTCGGGGGTGGCCTGGTCTGCCTTCCACAGC

ORF Protein

Cloned ORF protein sequence for pFN21AB7115 Download

>KIBB7115  279 aa
MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFND
ILVYGSIVLNKRKYRSQHIIPLEEVTLELLPETLQAKNRWMIKTAKKSFVVSAASATERQ
EWISHIEECVRRQLRATGRPPSTEHAAPWIPDKATDICMRCTQTRFSALTRRHHCRKCGF
VVCAECSRQRFLLPRLSPKPVRVCSLCYRELAAQQRQEEAEEQGAGSPGQPAHLARPICG
ASSGDDDDSDEDKEGSRDGDWPSSVEFYASGVAWSAFHS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7115 Download

>pFN21AB7115  5542 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGTGGACCACTTGGCCAACACGGAGATCAACAG
CCAGCGCATCGCGGCAGTGGAGAGCTGCTTCGGGGCCTCGGGGCAGCCGCTGGCGCTGCC
AGGCCGAGTGCTGCTGGGCGAGGGCGTGCTGACCAAAGAGTGCCGCAAGAAGGCCAAGCC
GCGCATCTTCTTCCTCTTTAACGACATCCTGGTGTATGGCAGCATCGTGCTCAACAAGCG
CAAGTACCGCAGCCAGCACATCATCCCCCTGGAGGAGGTCACACTGGAGCTGTTGCCGGA
GACGCTGCAGGCCAAGAACCGCTGGATGATCAAGACGGCCAAGAAGTCCTTTGTGGTGTC
GGCCGCCTCCGCTACGGAGCGCCAGGAATGGATTAGCCACATCGAGGAGTGCGTGCGGCG
GCAACTGAGGGCCACGGGCCGCCCGCCCAGCACGGAGCACGCGGCACCCTGGATCCCCGA
CAAGGCCACGGACATCTGCATGCGCTGCACGCAGACGCGCTTCTCTGCCCTCACGAGGCG
CCACCACTGCCGCAAGTGCGGCTTCGTGGTCTGCGCTGAGTGCTCGCGCCAGCGCTTCCT
GCTCCCGCGCCTGTCCCCCAAGCCCGTGCGCGTCTGCAGCCTCTGCTACCGCGAACTGGC
CGCCCAGCAGCGGCAGGAGGAGGCGGAGGAGCAGGGCGCGGGGTCCCCAGGGCAGCCAGC
CCACCTGGCCCGGCCCATCTGCGGAGCGTCCAGTGGAGATGACGATGACTCCGACGAGGA
CAAGGAGGGCAGCAGGGACGGCGACTGGCCCAGCAGCGTGGAGTTCTACGCCTCGGGGGT
GGCCTGGTCTGCCTTCCACAGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT