LGI3 ORF clone FHC01842

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC01842

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Clone info.

Species

human

Clone Name

pFN21AB7021 Sequence (with vector) Restriction Map

Product ID

FHC01842

Flexi Clone PID

FXC01842

ORF Sequence

DNA sequence (1644 bp)
Protein sequence (548 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385182]

Gene

KIBB7021

Symbol

LGI3

Alias : LGIL4

Description

leucine-rich repeat LGI family, member 3

Original Clone ID

hk10327b

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1644 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 548 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6025.1 6.5e-214 100.0 LGI3 CCDS7431.1 5.5e-115 52.0 LGI1 CCDS3431.1 4e-111 51.5 LGI2 CCDS81490.1 5.4e-90 46.2 LGI1 CCDS12444.1 1.6e-85 42.8 LGI4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR005492 221 263 PF03736 Leucine-rich glioma-inactivated IPR005492 269 309 PF03736 Leucine-rich glioma-inactivated IPR005492 313 360 PF03736 Leucine-rich glioma-inactivated IPR005492 362 405 PF03736 Leucine-rich glioma-inactivated IPR005492 409 453 PF03736 Leucine-rich glioma-inactivated IPR005492 454 497 PF03736 Leucine-rich glioma-inactivated IPR005492 500 540 PF03736 Leucine-rich glioma-inactivated HMMSmart IPR003591 87 110 SM00369 Leucine-rich repeat IPR003591 111 134 SM00369 Leucine-rich repeat IPR003591 135 158 SM00369 Leucine-rich repeat IPR000483 170 219 SM00082 Cysteine-rich flanking region ProfileScan IPR001611 89 110 PS51450 Leucine-rich repeat IPR001611 113 134 PS51450 Leucine-rich repeat IPR001611 137 158 PS51450 Leucine-rich repeat IPR001611 161 182 PS51450 Leucine-rich repeat IPR009039 221 264 PS50912 EAR IPR009039 267 310 PS50912 EAR IPR009039 313 361 PS50912 EAR IPR009039 362 406 PS50912 EAR IPR009039 409 453 PS50912 EAR IPR009039 454 497 PS50912 EAR IPR009039 500 543 PS50912 EAR
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_644807 548 608302 100.0 100.0 NP_005088 557 600512,604619 52.0 96.2 NP_060646 545 608301 51.5 98.7 NP_001295205 509 600512,604619 46.2 96.2 NP_644813 537 608303 42.8 98.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7021 Download

>KIBB7021  1644 bp
ATGGCGGGGCTGCGGGCCAGGGGGGGCCCGGGGCCGGGGCTGCTGGCGCTCTCCGCGCTC
GGCTTCTGCCTCATGCTGCAAGTCAGCGCTAAGAGGCCCCCCAAGACGCCCCCCTGCCCG
CCCAGCTGCTCTTGCACCAGGGACACCGCCTTCTGCGTGGACTCAAAGGCGGTGCCCAGG
AACCTGCCCTCGGAGGTCATCTCCCTGACCCTGGTGAATGCCGCCTTCTCAGAGATCCAG
GATGGAGCGTTCTCCCACCTCCCGCTGCTGCAGTTCTTGTTACTCAACTCCAACAAGTTT
ACACTGATTGGAGACAACGCCTTCACAGGACTGTCGCACCTGCAGTATCTCTTCATTGAG
AACAATGACATCTGGGCACTATCCAAGTTCACCTTCCGAGGACTCAAGTCCTTGACTCAC
CTCTCGCTGGCCAACAATAACCTGCAGACACTGCCCAGAGACATCTTCCGGCCCCTGGAC
ATCCTGAATGACTTGGACCTGCGGGGCAACTCACTCAACTGTGACTGCAAGGTGAAGTGG
TTGGTGGAGTGGCTGGCACACACCAACACCACGGTGGCACCCATCTACTGCGCCAGCCCG
CCCCGCTTCCAGGAGCACAAGGTGCAGGACCTGCCGCTGCGGGAGTTCGATTGCATCACC
ACAGATTTTGTGTTGTACCAGACCCTGGCCTTCCCAGCAGTGTCGGCTGAGCCCTTCCTC
TACTCCAGTGACCTCTATTTGGCTCTGGCCCAGCCAGGAGTCAGTGCCTGCACCATCCTG
AAGTGGGACTATGTTGAGCGGCAGCTTCGAGACTATGATAGAATCCCAGCCCCCTCTGCA
GTGCACTGCAAGCCGATGGTGGTGGACAGCCAGCTGTACGTGGTCGTGGCCCAGCTGTTT
GGCGGCTCTTACATTTACCACTGGGATCCCAACACCACGCGCTTCACCAGGCTGCAAGAC
ATTGACCCGCAGCGCGTGCGCAAGCCTAACGACCTAGAAGCCTTCCGCATCGACGGTGAC
TGGTACTTTGCCGTGGCTGACAGCTCCAAGGCAGGCGCCACCAGCCTCTACCGCTGGCAC
CAGAATGGCTTCTACTCCCACCAGGCACTGCACCCCTGGCACCGTGACACCGACCTGGAG
TTTGTGGACGGCGAGGGCAAGCCACGGCTGATTGTGTCCAGCAGCTCCCAGGCACCCGTC
ATCTATCAGTGGAGTCGCACCCAGAAGCAGTTTGTGGCCCAGGGTGAGGTGACCCAGGTG
CCTGATGCCCAAGCTGTGAAACACTTTCGTGCCGGCCGCGACAGCTACCTGTGCCTCAGC
CGCTACATTGGCGACTCCAAGATCCTGCGCTGGGAGGGTACCCGCTTCTCGGAGGTGCAG
GCCCTGCCCTCCCGGGGCTCGCTGGCCCTGCAGCCCTTCCTTGTGGGTGGCCGCCGCTAC
CTGGCACTGGGCAGTGATTTCTCCTTCACCCAGATCTACCAGTGGGATGAGGGACGACAG
AAGTTTGTACGGTTCCAGGAGCTGGCTGTGCAGGCTCCTCGGGCCTTCTGCTACATGCCT
GCTGGGGACGCCCAGCTACTCCTGGCCCCCAGCTTCAAGGGACAGACGCTGGTGTATAGA
CACATTGTGGTGGATCTCAGTGCC

ORF Protein

Cloned ORF protein sequence for pFN21AB7021 Download

>KIBB7021  548 aa
MAGLRARGGPGPGLLALSALGFCLMLQVSAKRPPKTPPCPPSCSCTRDTAFCVDSKAVPR
NLPSEVISLTLVNAAFSEIQDGAFSHLPLLQFLLLNSNKFTLIGDNAFTGLSHLQYLFIE
NNDIWALSKFTFRGLKSLTHLSLANNNLQTLPRDIFRPLDILNDLDLRGNSLNCDCKVKW
LVEWLAHTNTTVAPIYCASPPRFQEHKVQDLPLREFDCITTDFVLYQTLAFPAVSAEPFL
YSSDLYLALAQPGVSACTILKWDYVERQLRDYDRIPAPSAVHCKPMVVDSQLYVVVAQLF
GGSYIYHWDPNTTRFTRLQDIDPQRVRKPNDLEAFRIDGDWYFAVADSSKAGATSLYRWH
QNGFYSHQALHPWHRDTDLEFVDGEGKPRLIVSSSSQAPVIYQWSRTQKQFVAQGEVTQV
PDAQAVKHFRAGRDSYLCLSRYIGDSKILRWEGTRFSEVQALPSRGSLALQPFLVGGRRY
LALGSDFSFTQIYQWDEGRQKFVRFQELAVQAPRAFCYMPAGDAQLLLAPSFKGQTLVYR
HIVVDLSA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7021 Download

>pFN21AB7021  6349 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGGGCTGCGGGCCAGGGGGGGCCCGGGGCC
GGGGCTGCTGGCGCTCTCCGCGCTCGGCTTCTGCCTCATGCTGCAAGTCAGCGCTAAGAG
GCCCCCCAAGACGCCCCCCTGCCCGCCCAGCTGCTCTTGCACCAGGGACACCGCCTTCTG
CGTGGACTCAAAGGCGGTGCCCAGGAACCTGCCCTCGGAGGTCATCTCCCTGACCCTGGT
GAATGCCGCCTTCTCAGAGATCCAGGATGGAGCGTTCTCCCACCTCCCGCTGCTGCAGTT
CTTGTTACTCAACTCCAACAAGTTTACACTGATTGGAGACAACGCCTTCACAGGACTGTC
GCACCTGCAGTATCTCTTCATTGAGAACAATGACATCTGGGCACTATCCAAGTTCACCTT
CCGAGGACTCAAGTCCTTGACTCACCTCTCGCTGGCCAACAATAACCTGCAGACACTGCC
CAGAGACATCTTCCGGCCCCTGGACATCCTGAATGACTTGGACCTGCGGGGCAACTCACT
CAACTGTGACTGCAAGGTGAAGTGGTTGGTGGAGTGGCTGGCACACACCAACACCACGGT
GGCACCCATCTACTGCGCCAGCCCGCCCCGCTTCCAGGAGCACAAGGTGCAGGACCTGCC
GCTGCGGGAGTTCGATTGCATCACCACAGATTTTGTGTTGTACCAGACCCTGGCCTTCCC
AGCAGTGTCGGCTGAGCCCTTCCTCTACTCCAGTGACCTCTATTTGGCTCTGGCCCAGCC
AGGAGTCAGTGCCTGCACCATCCTGAAGTGGGACTATGTTGAGCGGCAGCTTCGAGACTA
TGATAGAATCCCAGCCCCCTCTGCAGTGCACTGCAAGCCGATGGTGGTGGACAGCCAGCT
GTACGTGGTCGTGGCCCAGCTGTTTGGCGGCTCTTACATTTACCACTGGGATCCCAACAC
CACGCGCTTCACCAGGCTGCAAGACATTGACCCGCAGCGCGTGCGCAAGCCTAACGACCT
AGAAGCCTTCCGCATCGACGGTGACTGGTACTTTGCCGTGGCTGACAGCTCCAAGGCAGG
CGCCACCAGCCTCTACCGCTGGCACCAGAATGGCTTCTACTCCCACCAGGCACTGCACCC
CTGGCACCGTGACACCGACCTGGAGTTTGTGGACGGCGAGGGCAAGCCACGGCTGATTGT
GTCCAGCAGCTCCCAGGCACCCGTCATCTATCAGTGGAGTCGCACCCAGAAGCAGTTTGT
GGCCCAGGGTGAGGTGACCCAGGTGCCTGATGCCCAAGCTGTGAAACACTTTCGTGCCGG
CCGCGACAGCTACCTGTGCCTCAGCCGCTACATTGGCGACTCCAAGATCCTGCGCTGGGA
GGGTACCCGCTTCTCGGAGGTGCAGGCCCTGCCCTCCCGGGGCTCGCTGGCCCTGCAGCC
CTTCCTTGTGGGTGGCCGCCGCTACCTGGCACTGGGCAGTGATTTCTCCTTCACCCAGAT
CTACCAGTGGGATGAGGGACGACAGAAGTTTGTACGGTTCCAGGAGCTGGCTGTGCAGGC
TCCTCGGGCCTTCTGCTACATGCCTGCTGGGGACGCCCAGCTACTCCTGGCCCCCAGCTT
CAAGGGACAGACGCTGGTGTATAGACACATTGTGGTGGATCTCAGTGCCGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT