ARHGEF25 ORF clone FHC00977

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00977

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Clone info.

Species

human

Clone Name

pFN21ASDB0010 Sequence (with vector) Restriction Map

Product ID

FHC00977

Flexi Clone PID

FXC00977

Original Clone PID

ORK00977

ORF Sequence

DNA sequence (1857 bp)
Protein sequence (619 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384356]

Gene

KIBB0010

Symbol

ARHGEF25

Alias : GEFT, p63RhoGEF

Description

Rho guanine nucleotide exchange factor (GEF) 25, transcript variant 3

Original Clone ID

bm00447

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1857 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 619 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS44931.1 9.8e-176 99.8 ARHGEF25 CCDS8947.1 5.3e-154 99.8 ARHGEF25 CCDS3883.1 1.3e-62 47.6 TRIO CCDS3028.1 4.1e-48 57.2 KALRN CCDS34124.1 1.3e-22 28.3 PLEKHG4B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000219 203 374 PF00621 Dbl homology (DH) domain HMMSmart IPR000219 203 374 SM00325 Dbl homology (DH) domain ProfileScan IPR000219 199 375 PS50010 Dbl homology (DH) domain IPR001849 387 505 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001104740 619 610215 100.0 100.0 NP_891992 580 610215 100.0 88.5 XP_016865292 2612 601893,617061 47.6 89.8 XP_016865291 2880 601893,617061 47.6 89.8 XP_011512412 3038 601893,617061 47.6 89.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDB0010 Download

>KIBB0010  1857 bp
ATGAAGCCCCCGGACCGCCCCGCCCCTGGCCGCACTGACCGGATACTGGGGGTCATGGGG
GGCATGCTGCGCGCATGCGCCCTCCCTGGGCAGGAGGGGCCCCCAAGGAGAAGCCCTCTA
GGGTTGGTGGGTACCGAGCCAGAGTCTGAACGTACGGAGGGAGATCACAGAAGGGATCGC
GAACATGAGGTCCTCGCCGGGGCTCTGCAGCCCGAATCCTATTCCATTGCGGGCAGTGAG
GGGAGTATATCGGCTTCTGCTGCCTCCGGTCTGGCTGCCCCCTCTGGCCCCAGCTCTGGC
CTCAGCTCTGGCCCCTGTTCCCCAGGCCCCCCAGGGCCCGTCAGTGGCCTGAGGAGATGG
TTGGATCATTCCAAACATTGTCTCAGTGTGGAAACTGAGGCAGACAGTGGTCAGGCAGGA
CCATATGAGAACTGGATGTTGGAGCCAGCTCTAGCCACAGGAGAGGAGCTGCCGGAACTG
ACCTTGCTGACCACACTGTTGGAGGGCCCTGGAGATAAGACGCAGCCACCTGAAGAGGAG
ACTTTGTCCCAAGCCCCTGAGAGTGAGGAGGAACAGAAGAAGAAGGCTCTGGAAAGGAGT
ATGTATGTCCTGAGTGAACTGGTAGAAACAGAGAAAATGTACGTGGACGACTTGGGGCAG
ATTGTGGAGGGTTATATGGCCACCATGGCTGCTCAGGGGGTCCCCGAGAGTCTTCGAGGC
CGTGACAGGATTGTGTTTGGGAATATCCAGCAAATCTATGAGTGGCACCGAGACTATTTC
TTGCAAGAGCTACAACGGTGTCTGAAAGATCCTGATTGGCTGGCTCAGCTATTCATCAAA
CACGAGCGCCGGCTGCATATGTATGTGGTGTACTGTCAGAATAAGCCCAAGTCAGAGCAT
GTGGTGTCAGAGTTTGGGGACAGCTACTTTGAGGAGCTCCGGCAGCAGCTGGGGCACCGC
CTGCAGCTGAACGACCTCCTCATCAAACCTGTGCAGCGGATCATGAAATACCAGCTGCTG
CTCAAGGATTTTCTCAAGTATTACAATAGAGCTGGGATGGATACTGCAGACCTAGAGCAA
GCTGTGGAGGTCATGTGCTTTGTGCCCAAGCGCTGCAACGATATGATGACGCTGGGGAGA
TTGCGGGGATTTGAGGGCAAACTGACTGCTCAGGGGAAGCTCTTGGGCCAGGACACTTTC
TGGGTCACCGAGCCTGAGGCTGGAGGGCTGCTATCTTCCCGAGGTCGAGAGAGGCGCGTC
TTCCTCTTTGAGCAAATCATCATCTTCAGTGAAGCCCTGGGAGGAGGAGTGAGAGGTGGA
ACACAGCCTGGATATGTATACAAGAACAGCATTAAGGTGAGCTGCCTGGGACTGGAGGGG
AACCTCCAAGGTGACCCTTGCCGCTTTGCACTGACCTCCAGAGGGCCAGAGGGTGGGATC
CAGCGCTATGTCCTGCAGGCTGCAGACCCTGCTATCAGTCAGGCCTGGATCAAGCATGTG
GCTCAGATCTTGGAGAGCCAACGGGACTTCCTCAACGCATTGCAGTCACCCATTGAGTAC
CAGAGACGGGAGAGCCAGACCAACAGCCTGGGGCGGCCAAGAGGGCCTGGAGTGGGGAGC
CCTGGAAGAATTCGGCTTGGAGATCAGGCCCAGGGCAGCACACACACACCCATCAATGGC
TCTCTCCCCTCTCTGCTGCTGTCACCCAAAGGGGAGGTGGCCAGAGCCCTCTTGCCACTG
GATAAACAGGCCCTTGGTGACATCCCCCAGGCTCCCCATGACTCTCCTCCAGTCTCTCCA
ACTCCAAAAACCCCTCCCTGCCAAGCCAGACTTGCCAAGCTGGATGAAGATGAGCTG

ORF Protein

Cloned ORF protein sequence for pFN21ASDB0010 Download

>KIBB0010  619 aa
MKPPDRPAPGRTDRILGVMGGMLRACALPGQEGPPRRSPLGLVGTEPESERTEGDHRRDR
EHEVLAGALQPESYSIAGSEGSISASAASGLAAPSGPSSGLSSGPCSPGPPGPVSGLRRW
LDHSKHCLSVETEADSGQAGPYENWMLEPALATGEELPELTLLTTLLEGPGDKTQPPEEE
TLSQAPESEEEQKKKALERSMYVLSELVETEKMYVDDLGQIVEGYMATMAAQGVPESLRG
RDRIVFGNIQQIYEWHRDYFLQELQRCLKDPDWLAQLFIKHERRLHMYVVYCQNKPKSEH
VVSEFGDSYFEELRQQLGHRLQLNDLLIKPVQRIMKYQLLLKDFLKYYNRAGMDTADLEQ
AVEVMCFVPKRCNDMMTLGRLRGFEGKLTAQGKLLGQDTFWVTEPEAGGLLSSRGRERRV
FLFEQIIIFSEALGGGVRGGTQPGYVYKNSIKVSCLGLEGNLQGDPCRFALTSRGPEGGI
QRYVLQAADPAISQAWIKHVAQILESQRDFLNALQSPIEYQRRESQTNSLGRPRGPGVGS
PGRIRLGDQAQGSTHTPINGSLPSLLLSPKGEVARALLPLDKQALGDIPQAPHDSPPVSP
TPKTPPCQARLAKLDEDEL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDB0010 Download

>pFN21ASDB0010  6586 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGAAGCCCCCGGACCG
CCCCGCCCCTGGCCGCACTGACCGGATACTGGGGGTCATGGGGGGCATGCTGCGCGCATG
CGCCCTCCCTGGGCAGGAGGGGCCCCCAAGGAGAAGCCCTCTAGGGTTGGTGGGTACCGA
GCCAGAGTCTGAACGTACGGAGGGAGATCACAGAAGGGATCGCGAACATGAGGTCCTCGC
CGGGGCTCTGCAGCCCGAATCCTATTCCATTGCGGGCAGTGAGGGGAGTATATCGGCTTC
TGCTGCCTCCGGTCTGGCTGCCCCCTCTGGCCCCAGCTCTGGCCTCAGCTCTGGCCCCTG
TTCCCCAGGCCCCCCAGGGCCCGTCAGTGGCCTGAGGAGATGGTTGGATCATTCCAAACA
TTGTCTCAGTGTGGAAACTGAGGCAGACAGTGGTCAGGCAGGACCATATGAGAACTGGAT
GTTGGAGCCAGCTCTAGCCACAGGAGAGGAGCTGCCGGAACTGACCTTGCTGACCACACT
GTTGGAGGGCCCTGGAGATAAGACGCAGCCACCTGAAGAGGAGACTTTGTCCCAAGCCCC
TGAGAGTGAGGAGGAACAGAAGAAGAAGGCTCTGGAAAGGAGTATGTATGTCCTGAGTGA
ACTGGTAGAAACAGAGAAAATGTACGTGGACGACTTGGGGCAGATTGTGGAGGGTTATAT
GGCCACCATGGCTGCTCAGGGGGTCCCCGAGAGTCTTCGAGGCCGTGACAGGATTGTGTT
TGGGAATATCCAGCAAATCTATGAGTGGCACCGAGACTATTTCTTGCAAGAGCTACAACG
GTGTCTGAAAGATCCTGATTGGCTGGCTCAGCTATTCATCAAACACGAGCGCCGGCTGCA
TATGTATGTGGTGTACTGTCAGAATAAGCCCAAGTCAGAGCATGTGGTGTCAGAGTTTGG
GGACAGCTACTTTGAGGAGCTCCGGCAGCAGCTGGGGCACCGCCTGCAGCTGAACGACCT
CCTCATCAAACCTGTGCAGCGGATCATGAAATACCAGCTGCTGCTCAAGGATTTTCTCAA
GTATTACAATAGAGCTGGGATGGATACTGCAGACCTAGAGCAAGCTGTGGAGGTCATGTG
CTTTGTGCCCAAGCGCTGCAACGATATGATGACGCTGGGGAGATTGCGGGGATTTGAGGG
CAAACTGACTGCTCAGGGGAAGCTCTTGGGCCAGGACACTTTCTGGGTCACCGAGCCTGA
GGCTGGAGGGCTGCTATCTTCCCGAGGTCGAGAGAGGCGCGTCTTCCTCTTTGAGCAAAT
CATCATCTTCAGTGAAGCCCTGGGAGGAGGAGTGAGAGGTGGAACACAGCCTGGATATGT
ATACAAGAACAGCATTAAGGTGAGCTGCCTGGGACTGGAGGGGAACCTCCAAGGTGACCC
TTGCCGCTTTGCACTGACCTCCAGAGGGCCAGAGGGTGGGATCCAGCGCTATGTCCTGCA
GGCTGCAGACCCTGCTATCAGTCAGGCCTGGATCAAGCATGTGGCTCAGATCTTGGAGAG
CCAACGGGACTTCCTCAACGCATTGCAGTCACCCATTGAGTACCAGAGACGGGAGAGCCA
GACCAACAGCCTGGGGCGGCCAAGAGGGCCTGGAGTGGGGAGCCCTGGAAGAATTCGGCT
TGGAGATCAGGCCCAGGGCAGCACACACACACCCATCAATGGCTCTCTCCCCTCTCTGCT
GCTGTCACCCAAAGGGGAGGTGGCCAGAGCCCTCTTGCCACTGGATAAACAGGCCCTTGG
TGACATCCCCCAGGCTCCCCATGACTCTCCTCCAGTCTCTCCAACTCCAAAAACCCCTCC
CTGCCAAGCCAGACTTGCCAAGCTGGATGAAGATGAGCTGTACGTAGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT