DCUN1D4 ORF clone FHC00489

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Halotag ORF Clone FHC00489

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Clone info.

Species

human

Clone Name

pFN21ASDA0276 Sequence (with vector) Restriction Map

Product ID

FHC00489

Flexi Clone PID

FXC00489

ORF Sequence

DNA sequence (876 bp)
Protein sequence (292 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383832]

Gene

KIAA0276

Symbol

DCUN1D4

Alias : DCNL4

Description

DCN1, defective in cullin neddylation 1, domain containing 4, transcript variant 1

Original Clone ID

ha06604

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 876 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 292 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33982.1 2.3e-126 100.0 DCUN1D4 CCDS75123.1 1.2e-122 99.6 DCUN1D4 CCDS3487.2 2.9e-86 88.0 DCUN1D4 CCDS8325.1 2.3e-69 70.0 DCUN1D5 CCDS32013.1 2.1e-26 34.8 DCUN1D2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR005176 174 287 PF03556 Domain of unknown function DUF298 ProfileScan IPR005176 101 287 PS51229 Domain of unknown function DUF298
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001035492 292 612977 100.0 100.0 XP_011532681 294 612977 99.6 97.3 NP_001274684 336 612977 99.6 97.3 NP_055930 257 612977 88.0 100.0 XP_016863401 259 612977 87.3 97.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0276 Download

>KIAA0276  876 bp
ATGCACTCGGATGCCGCCGCTGTCAATTTTCAGCTGAACTCTCATCTCTCAACACTGGCA
AATATTCATAAGATCTACCACACCCTTAATAAGCTGAACCTAACAGAAGACATTGGCCAA
GACGATCACCAAACAGGAAGTCTGCGGTCTTGCAGTTCTTCAGACTGCTTTAATAAAGTG
ATGCCACCAAGGAAAAAGAGAAGACCTGCCTCTGGAGATGATTTATCTGCCAAGAAAAGT
AGACATGATAGCATGTATAGAAAATATGATTCGACTAGAATAAAGACTGAAGAAGAAGCC
TTTTCAAGTAAAAGGTGCTTGGAATGGTTCTATGAATATGCAGGAACTGATGATGTTGTA
GGCCCTGAAGGCATGGAGAAATTTTGTGAAGACATTGGTGTTGAACCAGAAAACGTAGTT
ATGCTTGTCCTAGCTTGGAAATTGGATGCACAAAACATGGGTTATTTTACTCTACAGGAG
TGGTTAAAAGGAATGACTTCTCTCCAATGTGATACAACAGAAAAACTCAGAAATACTTTG
GATTACTTAAGATCATTCTTAAATGATTCTACAAACTTTAAACTTATTTACAGATATGCG
TTTGACTTTGCACGGGAAAAGGACCAGCGCAGCCTAGACATAAACACTGCCAAGTGCATG
TTGGGACTGTTATTAGGAAAAATCTGGCCCCTTTTTCCAGTTTTTCACCAATTCTTAGAG
CAATCAAAATACAAAGTTATTAATAAAGACCAGTGGTGCAATGTCCTAGAGTTTAGCAGA
ACAATTAATCTTGACCTCAGCAACTATGATGAAGATGGAGCATGGCCAGTTTTGTTGGAC
GAGTTTGTGGAGTGGTATAAAGACAAACAGATGTCC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0276 Download

>KIAA0276  292 aa
MHSDAAAVNFQLNSHLSTLANIHKIYHTLNKLNLTEDIGQDDHQTGSLRSCSSSDCFNKV
MPPRKKRRPASGDDLSAKKSRHDSMYRKYDSTRIKTEEEAFSSKRCLEWFYEYAGTDDVV
GPEGMEKFCEDIGVEPENVVMLVLAWKLDAQNMGYFTLQEWLKGMTSLQCDTTEKLRNTL
DYLRSFLNDSTNFKLIYRYAFDFAREKDQRSLDINTAKCMLGLLLGKIWPLFPVFHQFLE
QSKYKVINKDQWCNVLEFSRTINLDLSNYDEDGAWPVLLDEFVEWYKDKQMS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0276 Download

>pFN21ASDA0276  5605 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGCACTCGGATGCCGC
CGCTGTCAATTTTCAGCTGAACTCTCATCTCTCAACACTGGCAAATATTCATAAGATCTA
CCACACCCTTAATAAGCTGAACCTAACAGAAGACATTGGCCAAGACGATCACCAAACAGG
AAGTCTGCGGTCTTGCAGTTCTTCAGACTGCTTTAATAAAGTGATGCCACCAAGGAAAAA
GAGAAGACCTGCCTCTGGAGATGATTTATCTGCCAAGAAAAGTAGACATGATAGCATGTA
TAGAAAATATGATTCGACTAGAATAAAGACTGAAGAAGAAGCCTTTTCAAGTAAAAGGTG
CTTGGAATGGTTCTATGAATATGCAGGAACTGATGATGTTGTAGGCCCTGAAGGCATGGA
GAAATTTTGTGAAGACATTGGTGTTGAACCAGAAAACGTAGTTATGCTTGTCCTAGCTTG
GAAATTGGATGCACAAAACATGGGTTATTTTACTCTACAGGAGTGGTTAAAAGGAATGAC
TTCTCTCCAATGTGATACAACAGAAAAACTCAGAAATACTTTGGATTACTTAAGATCATT
CTTAAATGATTCTACAAACTTTAAACTTATTTACAGATATGCGTTTGACTTTGCACGGGA
AAAGGACCAGCGCAGCCTAGACATAAACACTGCCAAGTGCATGTTGGGACTGTTATTAGG
AAAAATCTGGCCCCTTTTTCCAGTTTTTCACCAATTCTTAGAGCAATCAAAATACAAAGT
TATTAATAAAGACCAGTGGTGCAATGTCCTAGAGTTTAGCAGAACAATTAATCTTGACCT
CAGCAACTATGATGAAGATGGAGCATGGCCAGTTTTGTTGGACGAGTTTGTGGAGTGGTA
TAAAGACAAACAGATGTCCTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT