MRPS27 ORF clone FHC00483

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Halotag ORF Clone FHC00483

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Clone info.

Species

human

Clone Name

pFN21ASDA0264 Sequence (with vector) Restriction Map

Product ID

FHC00483

Flexi Clone PID

FXC00483

ORF Sequence

DNA sequence (1242 bp)
Protein sequence (414 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383826]

Gene

KIAA0264

Symbol

MRPS27

Alias : MRP-S27, S27mt

Description

mitochondrial ribosomal protein S27, transcript variant 2

Original Clone ID

ha07040

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1242 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 414 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4013.1 2.1e-142 99.8 MRPS27 CCDS75257.1 1.9e-122 99.7 MRPS27 CCDS68890.1 2e-109 96.5 MRPS27 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019266 1 413 PF10037 Ribosomal protein S27 ProfileScan IPR002885 140 175 PS51375 Pentatricopeptide repeat
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055899 414 611989 99.8 100.0 NP_001273680 358 611989 99.7 86.5 NP_001273677 428 611989 96.5 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0264 Download

>KIAA0264  1242 bp
ATGGCTGCCTCCATAGTGCGGCGCGGGATGCTCCTGGCGCGGCAAGTGGTTCTTCCTCAG
CTCTCTCCTGCAGGTAAAAGATACCTGCTTTCTTCAGCCTATGTAGACAGCCACAAATGG
GAAGCAAGAGAAAAAGAACATTACTGTCTTGCTGATCTTGCATCTTTAATGGATAAAACA
TTTGAGAGAAAGTTGCCTGTTAGTTCTTTAACAATATCACGGCTTATAGACAACATTTCC
TCTCGGGAAGAGATAGATCATGCAGAGTATTACCTTTACAAGTTTCGACACAGCCCCAAC
TGCTGGTACCTGAGAAACTGGACTATCCACACCTGGATTAGGCAGTGTCTAAAATATGAT
GCACAAGACAAAGCCCTATATACCCTTGTAAATAAGGTTCAATATGGAATTTTTCCAGAT
AACTTTACATTCAATTTACTGATGGATTCTTTCATAAAGAAAGAAAATTACAAAGATGCT
TTATCTGTGGTTTTTGAGGTCATGATGCAAGAAGCCTTTGAAGTGCCTTCCACCCAACTT
CTCTCCCTCTATGTTTTATTTCATTGCCTGGCAAAGAAGACAGACTTCAGTTGGGAAGAG
GAGAGGAACTTTGGTGCATCCCTTTTGCTTCCAGGCCTAAAACAAAAGAACTCAGTGGGT
TTCAGTTCCCAGTTGTATGGCTATGCACTTCTTGGGAAGGTGGAGTTGCAGCAAGGGCTA
CGGGCTGTGTACCACAACATGCCTCTGATATGGAAACCAGGCTACCTTGACAGAGCCCTT
CAAGTGATGGAGAAAGTGGCTGCCTCCCCAGAAGACATAAAGCTGTGTAGAGAAGCGCTC
GATGTGCTGGATGCAGTGCTGAAGGCTCTGACTTCAGCTGATGGGGCTTCAGAGGAGCAG
TCCCAAAATGATGAAGACAACCAGGGGTCAGAAAAACTGGTGGAGCAGTTAGACATCGAG
GAAACAGAGCAGTCCAAGCTTCCTCAATACCTGGAACGATTTAAGGCCTTACATTCTAAG
CTTCAAGCTCTGGGCAAAATTGAGTCAGAAGGTCTTTTAAGTCTGACCACCCAGCTTGTC
AAGGAAAAACTCTCCACCTGTGAAGCAGAGGACATCGCCACCTATGAGCAGAATCTGCAG
CAGTGGCATCTAGACCTTGTACAGTTGATCCAGAGAGAACAGCAACAGAGGGAGCAAGCG
AAGCAGGAGTACCAGGCTCAGAAAGCAGCAAAGGCATCTGCC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0264 Download

>KIAA0264  414 aa
MAASIVRRGMLLARQVVLPQLSPAGKRYLLSSAYVDSHKWEAREKEHYCLADLASLMDKT
FERKLPVSSLTISRLIDNISSREEIDHAEYYLYKFRHSPNCWYLRNWTIHTWIRQCLKYD
AQDKALYTLVNKVQYGIFPDNFTFNLLMDSFIKKENYKDALSVVFEVMMQEAFEVPSTQL
LSLYVLFHCLAKKTDFSWEEERNFGASLLLPGLKQKNSVGFSSQLYGYALLGKVELQQGL
RAVYHNMPLIWKPGYLDRALQVMEKVAASPEDIKLCREALDVLDAVLKALTSADGASEEQ
SQNDEDNQGSEKLVEQLDIEETEQSKLPQYLERFKALHSKLQALGKIESEGLLSLTTQLV
KEKLSTCEAEDIATYEQNLQQWHLDLVQLIQREQQQREQAKQEYQAQKAAKASA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0264 Download

>pFN21ASDA0264  5971 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCTGCCTCCATAGT
GCGGCGCGGGATGCTCCTGGCGCGGCAAGTGGTTCTTCCTCAGCTCTCTCCTGCAGGTAA
AAGATACCTGCTTTCTTCAGCCTATGTAGACAGCCACAAATGGGAAGCAAGAGAAAAAGA
ACATTACTGTCTTGCTGATCTTGCATCTTTAATGGATAAAACATTTGAGAGAAAGTTGCC
TGTTAGTTCTTTAACAATATCACGGCTTATAGACAACATTTCCTCTCGGGAAGAGATAGA
TCATGCAGAGTATTACCTTTACAAGTTTCGACACAGCCCCAACTGCTGGTACCTGAGAAA
CTGGACTATCCACACCTGGATTAGGCAGTGTCTAAAATATGATGCACAAGACAAAGCCCT
ATATACCCTTGTAAATAAGGTTCAATATGGAATTTTTCCAGATAACTTTACATTCAATTT
ACTGATGGATTCTTTCATAAAGAAAGAAAATTACAAAGATGCTTTATCTGTGGTTTTTGA
GGTCATGATGCAAGAAGCCTTTGAAGTGCCTTCCACCCAACTTCTCTCCCTCTATGTTTT
ATTTCATTGCCTGGCAAAGAAGACAGACTTCAGTTGGGAAGAGGAGAGGAACTTTGGTGC
ATCCCTTTTGCTTCCAGGCCTAAAACAAAAGAACTCAGTGGGTTTCAGTTCCCAGTTGTA
TGGCTATGCACTTCTTGGGAAGGTGGAGTTGCAGCAAGGGCTACGGGCTGTGTACCACAA
CATGCCTCTGATATGGAAACCAGGCTACCTTGACAGAGCCCTTCAAGTGATGGAGAAAGT
GGCTGCCTCCCCAGAAGACATAAAGCTGTGTAGAGAAGCGCTCGATGTGCTGGATGCAGT
GCTGAAGGCTCTGACTTCAGCTGATGGGGCTTCAGAGGAGCAGTCCCAAAATGATGAAGA
CAACCAGGGGTCAGAAAAACTGGTGGAGCAGTTAGACATCGAGGAAACAGAGCAGTCCAA
GCTTCCTCAATACCTGGAACGATTTAAGGCCTTACATTCTAAGCTTCAAGCTCTGGGCAA
AATTGAGTCAGAAGGTCTTTTAAGTCTGACCACCCAGCTTGTCAAGGAAAAACTCTCCAC
CTGTGAAGCAGAGGACATCGCCACCTATGAGCAGAATCTGCAGCAGTGGCATCTAGACCT
TGTACAGTTGATCCAGAGAGAACAGCAACAGAGGGAGCAAGCGAAGCAGGAGTACCAGGC
TCAGAAAGCAGCAAAGGCATCTGCCTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT