(Circular) (Six-base) MAPSORT of: pF1KE2583.seq Check: 8675 from: 1 to: 8039 pF1KE2583 8039 bp With 182 enzymes: SgfI * August 16, 2021 14:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2413 2413 Size: 8039 AccI GT'mk_AC Cuts at: 1646 5077 1646 Size: 3431 4608 Acc65I G'GTAC_C Cuts at: 5055 5055 Size: 8039 AclI AA'CG_TT Cuts at: 7879 7879 Size: 8039 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2491 2893 4292 4781 5864 6296 7043 7311 2491 Size: 402 1399 489 1083 432 747 268 3219 Fragments arranged by size: 3219 1399 1083 747 489 432 402 268 AfeI AGC'GCT Cuts at: 4023 4023 Size: 8039 AflIII A'CryG_T Cuts at: 5391 6495 7472 5391 Size: 1104 977 5958 Fragments arranged by size: 5958 1104 977 AgeI A'CCGG_T Cuts at: 6427 6427 Size: 8039 AhdI GACnn_n'nnGTC Cuts at: 1089 1089 Size: 8039 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2431 2431 Size: 8039 AlwNI CAG_nnn'CTG Cuts at: 2418 2614 5026 5344 6911 2418 Size: 196 2412 318 1567 3546 Fragments arranged by size: 3546 2412 1567 318 196 ApaI G_GGCC'C Cuts at: 997 1412 997 Size: 415 7624 ApaLI G'TGCA_C Cuts at: 6809 6809 Size: 8039 ApoI r'AATT_y Cuts at: 403 2518 2689 5043 7392 7648 403 Size: 2115 171 2354 2349 256 794 Fragments arranged by size: 2354 2349 2115 794 256 171 AseI AT'TA_AT Cuts at: 20 3302 20 Size: 3282 4757 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 8039 AvaI C'yCGr_G Cuts at: 3965 4553 5059 3965 Size: 588 506 6945 Fragments arranged by size: 6945 588 506 AvrII C'CTAG_G Cuts at: 4894 4894 Size: 8039 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 1313 1346 3030 3063 1313 Size: 33 1684 33 6289 Fragments arranged by size: 6289 1684 33 33 BamHI G'GATC_C Cuts at: 5064 5064 Size: 8039 BanI G'GyrC_C Cuts at: 868 886 1437 3367 5055 5469 5717 5752 868 Size: 18 551 1930 1688 414 248 35 3155 Fragments arranged by size: 3155 1930 1688 551 414 248 35 18 BanII G_rGCy'C Cuts at: 115 130 142 997 1412 1829 5053 6083 115 Size: 15 12 855 415 417 3224 1030 2071 Fragments arranged by size: 3224 2071 1030 855 417 415 15 12 BbeI G_GCGC'C Cuts at: 890 3371 5473 5721 890 Size: 2481 2102 248 3208 Fragments arranged by size: 3208 2481 2102 248 BbsI GAAGACnn'nnnn_ Cuts at: 764 2448 4186 764 Size: 1684 1738 4617 Fragments arranged by size: 4617 1738 1684 BciVI GTATCCnnnnn_n' Cuts at: 459 2063 3370 4074 5937 6698 8039 459 Size: 1604 1307 704 1863 761 1341 459 Fragments arranged by size: 1863 1604 1341 1307 761 704 459 BclI T'GATC_A Cuts at: 596 3557 5559 596 Size: 2961 2002 3076 Fragments arranged by size: 3076 2961 2002 BglI GCCn_nnn'nGGC Cuts at: 1416 2155 1416 Size: 739 7300 BglII A'GATC_T Cuts at: 1394 2681 2931 4930 5554 7591 1394 Size: 1287 250 1999 624 2037 1842 Fragments arranged by size: 2037 1999 1842 1287 624 250 BlpI GC'TnA_GC Cuts at: 1348 3878 4712 5151 1348 Size: 2530 834 439 4236 Fragments arranged by size: 4236 2530 834 439 Bme1580I G_kGCm'C Cuts at: 968 997 1412 4213 5664 5757 6813 968 Size: 29 415 2801 1451 93 1056 2194 Fragments arranged by size: 2801 2194 1451 1056 415 93 29 BmgBI CAC'GTC Cuts at: 1075 4097 4158 1075 Size: 3022 61 4956 Fragments arranged by size: 4956 3022 61 BmrI ACTGGGnnnn_n' Cuts at: 961 1602 1744 4216 5251 5332 5667 7811 961 Size: 641 142 2472 1035 81 335 2144 1189 Fragments arranged by size: 2472 2144 1189 1035 641 335 142 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2878 3222 6406 2878 Size: 344 3184 4511 Fragments arranged by size: 4511 3184 344 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 529 1322 1732 1855 1949 2361 2785 3365 529 Size: 793 410 123 94 412 424 580 5203 Fragments arranged by size: 5203 793 580 424 412 410 123 94 Bpu10I CC'TnA_GC Cuts at: 3664 3664 Size: 8039 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 118 2604 5215 6094 6586 6884 118 Size: 2486 2611 879 492 298 1273 Fragments arranged by size: 2611 2486 1273 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 982 7743 982 Size: 6761 1278 BsaAI yAC'GTr Cuts at: 666 3090 5392 666 Size: 2424 2302 3313 Fragments arranged by size: 3313 2424 2302 BsaBI GATnn'nnATC Cuts at: 6 2815 3112 4860 5553 7590 6 Size: 2809 297 1748 693 2037 455 Fragments arranged by size: 2809 2037 1748 693 455 297 BsaHI Gr'CG_yC Cuts at: 887 3368 5470 5718 6420 7916 887 Size: 2481 2102 248 702 1496 1010 Fragments arranged by size: 2481 2102 1496 1010 702 248 BsaWI w'CCGG_w Cuts at: 5228 5749 6427 6701 6848 7259 5228 Size: 521 678 274 147 411 6008 Fragments arranged by size: 6008 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 676 706 2176 2206 676 Size: 30 1470 30 6509 Fragments arranged by size: 6509 1470 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 681 1729 1865 2650 3211 3349 3688 3715 681 Size: 1048 136 785 561 138 339 27 5005 Fragments arranged by size: 5005 1048 785 561 339 138 136 27 BseYI C'CCAG_C Cuts at: 298 5464 6222 6799 7295 298 Size: 5166 758 577 496 1042 Fragments arranged by size: 5166 1042 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 3733 3733 Size: 8039 BsiEI CG_ry'CG Cuts at: 104 3603 5581 5627 6835 104 Size: 3499 1978 46 1208 1308 Fragments arranged by size: 3499 1978 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 115 142 830 2648 3155 3722 5053 5831 6021 Size: 27 688 1818 507 567 1331 778 190 Cuts at: 6021 6813 115 Size: 792 1341 Fragments arranged by size: 1818 1341 1331 792 778 688 567 507 190 27 BsmI GAATG_Cn' Cuts at: 1605 3915 5035 1605 Size: 2310 1120 4609 Fragments arranged by size: 4609 2310 1120 BsmBI CGTCTCn'nnnn_ Cuts at: 3598 3598 Size: 8039 Bsp1286I G_dGCh'C Cuts at: 115 130 142 830 968 997 1412 1829 2648 Size: 15 12 688 138 29 415 417 819 Cuts at: 2648 3155 3722 4213 5053 5664 5757 5831 6021 Size: 507 567 491 840 611 93 74 190 Cuts at: 6021 6083 6813 115 Size: 62 730 1341 Fragments arranged by size: 1341 840 819 730 688 611 567 507 491 417 415 190 138 93 74 62 29 15 12 BspEI T'CCGG_A Cuts at: 7259 7259 Size: 8039 BspHI T'CATG_A Cuts at: 2954 3155 3528 7215 2954 Size: 201 373 3687 3778 Fragments arranged by size: 3778 3687 373 201 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1034 2362 2413 2756 3440 5089 5303 5605 5986 Size: 1328 51 343 684 1649 214 302 381 Cuts at: 5986 1034 Size: 3087 Fragments arranged by size: 3087 1649 1328 684 381 343 302 214 51 BsrBI CCG'CTC Cuts at: 50 6331 6385 7869 8034 50 Size: 6281 54 1484 165 55 Fragments arranged by size: 6281 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2393 4642 5951 2393 Size: 2249 1309 4481 Fragments arranged by size: 4481 2249 1309 BsrFI r'CCGG_y Cuts at: 6037 6218 6427 6037 Size: 181 209 7649 Fragments arranged by size: 7649 209 181 BssSI C'ACGA_G Cuts at: 970 979 1828 2062 2185 2935 6310 6668 970 Size: 9 849 234 123 750 3375 358 2341 Fragments arranged by size: 3375 2341 849 750 358 234 123 9 BstAPI GCAn_nnn'nTGC Cuts at: 963 1617 3711 5416 963 Size: 654 2094 1705 3586 Fragments arranged by size: 3586 2094 1705 654 BstBI TT'CG_AA Cuts at: 6400 6400 Size: 8039 BstXI CCAn_nnnn'nTGG Cuts at: 1017 1050 2151 1017 Size: 33 1101 6905 Fragments arranged by size: 6905 1101 33 BstYI r'GATC_y Cuts at: 1 626 1394 2681 2858 2931 4930 5064 5531 Size: 625 768 1287 177 73 1999 134 467 Cuts at: 5531 5554 5889 6135 7147 7233 7245 7591 1 Size: 23 335 246 1012 86 12 346 449 Fragments arranged by size: 1999 1287 1012 768 625 467 449 346 335 246 177 134 86 73 23 12 Bsu36I CC'TnA_GG Cuts at: 1141 1141 Size: 8039 BtgI C'CryG_G Cuts at: 733 1484 2177 2369 3293 733 Size: 751 693 192 924 5479 Fragments arranged by size: 5479 924 751 693 192 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1630 5989 6170 6333 1630 Size: 4359 181 163 3336 Fragments arranged by size: 4359 3336 181 163 BtsI GCAGTG_nn' Cuts at: 472 2087 2114 3712 4709 5282 472 Size: 1615 27 1598 997 573 3229 Fragments arranged by size: 3229 1615 1598 997 573 27 ClaI AT'CG_AT Cuts at: 4044 4044 Size: 8039 DraI TTT'AAA Cuts at: 4367 5038 4367 Size: 671 7368 DrdI GACnn_nn'nnGTC Cuts at: 3538 5745 6603 3538 Size: 2207 858 4974 Fragments arranged by size: 4974 2207 858 EaeI y'GGCC_r Cuts at: 252 1417 1487 2141 2253 2366 2791 2939 3003 Size: 1165 70 654 112 113 425 148 64 Cuts at: 3003 3600 5624 5798 6189 6216 7561 252 Size: 597 2024 174 391 27 1345 730 Fragments arranged by size: 2024 1345 1165 730 654 597 425 391 174 148 113 112 70 64 27 EagI C'GGCC_G Cuts at: 3600 5624 3600 Size: 2024 6015 EarI CTCTTCn'nnn_ Cuts at: 305 1028 1500 2000 2126 2579 3664 4058 4142 Size: 723 472 500 126 453 1085 394 84 Cuts at: 4142 4268 6062 6272 305 Size: 126 1794 210 2072 Fragments arranged by size: 2072 1794 1085 723 500 472 453 394 210 126 126 84 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 431 1136 1781 6247 6557 6703 7849 431 Size: 705 645 4466 310 146 1146 621 Fragments arranged by size: 4466 1146 705 645 621 310 146 EcoICRI GAG'CTC Cuts at: 113 140 5051 113 Size: 27 4911 3101 Fragments arranged by size: 4911 3101 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 529 1322 1732 1855 1949 2361 2491 2785 2893 Size: 793 410 123 94 412 130 294 108 Cuts at: 2893 3365 4292 4781 5864 6296 7043 7311 529 Size: 472 927 489 1083 432 747 268 1257 Fragments arranged by size: 1257 1083 927 793 747 489 472 432 412 410 294 268 130 123 108 94 EcoNI CCTnn'n_nnAGG Cuts at: 598 3952 598 Size: 3354 4685 EcoO109I rG'GnC_Cy Cuts at: 620 993 994 1409 2148 5178 620 Size: 373 1 415 739 3030 3481 Fragments arranged by size: 3481 3030 739 415 373 1 EcoRI G'AATT_C Cuts at: 403 2689 5043 403 Size: 2286 2354 3399 Fragments arranged by size: 3399 2354 2286 EcoRV GAT'ATC Cuts at: 3027 3508 4048 3027 Size: 481 540 7018 Fragments arranged by size: 7018 540 481 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2923 3851 6058 2923 Size: 928 2207 4904 Fragments arranged by size: 4904 2207 928 FspI TGC'GCA Cuts at: 1100 5820 1100 Size: 4720 3319 HaeII r_GCGC'y Cuts at: 890 3371 4025 5473 5721 6743 7722 890 Size: 2481 654 1448 248 1022 979 1207 Fragments arranged by size: 2481 1448 1207 1022 979 654 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 334 366 676 708 1075 1107 2203 2235 2524 Size: 32 310 32 367 32 1096 32 289 Cuts at: 2524 2556 2602 2634 3641 3673 3733 3765 4069 Size: 32 46 32 1007 32 60 32 304 Cuts at: 4069 4101 5881 5911 5913 5943 334 Size: 32 1780 30 2 30 2430 Fragments arranged by size: 2430 1780 1096 1007 367 310 304 289 60 46 32 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1647 3069 3864 4581 5078 1647 Size: 1422 795 717 497 4608 Fragments arranged by size: 4608 1422 795 717 497 HindIII A'AGCT_T Cuts at: 4539 5012 4539 Size: 473 7566 HpaI GTT'AAC Cuts at: 4581 4581 Size: 8039 KasI G'GCGC_C Cuts at: 886 3367 5469 5717 886 Size: 2481 2102 248 3208 Fragments arranged by size: 3208 2481 2102 248 KpnI G_GTAC'C Cuts at: 5059 5059 Size: 8039 MfeI C'AATT_G Cuts at: 4618 4618 Size: 8039 MluI A'CGCG_T Cuts at: 7472 7472 Size: 8039 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 747 827 3506 3791 4673 6710 6894 747 Size: 80 2679 285 882 2037 184 1892 Fragments arranged by size: 2679 2037 1892 882 285 184 80 MscI TGG'CCA Cuts at: 254 1489 2143 2941 5800 254 Size: 1235 654 798 2859 2493 Fragments arranged by size: 2859 2493 1235 798 654 MslI CAynn'nnrTG Cuts at: 1048 1291 2020 2773 2953 3586 6028 6155 1048 Size: 243 729 753 180 633 2442 127 2932 Fragments arranged by size: 2932 2442 753 729 633 243 180 127 MspA1I CmG'CkG Cuts at: 504 1070 1206 1347 1862 3513 3599 3605 5150 Size: 566 136 141 515 1651 86 6 1545 Cuts at: 5150 5464 5824 6837 7082 504 Size: 314 360 1013 245 1461 Fragments arranged by size: 1651 1545 1461 1013 566 515 360 314 245 141 136 86 6 NaeI GCC'GGC Cuts at: 6220 6220 Size: 8039 NarI GG'CG_CC Cuts at: 887 3368 5470 5718 887 Size: 2481 2102 248 3208 Fragments arranged by size: 3208 2481 2102 248 NcoI C'CATG_G Cuts at: 733 1484 2177 3293 733 Size: 751 693 1116 5479 Fragments arranged by size: 5479 1116 751 693 NdeI CA'TA_TG Cuts at: 2529 2529 Size: 8039 NgoMIV G'CCGG_C Cuts at: 6218 6218 Size: 8039 NotI GC'GGCC_GC Cuts at: 3600 3600 Size: 8039 NspI r_CATG'y Cuts at: 1686 3123 3240 4490 4942 5092 5592 6499 1686 Size: 1437 117 1250 452 150 500 907 3226 Fragments arranged by size: 3226 1437 1250 907 500 452 150 117 PasI CC'CwG_GG Cuts at: 873 1425 873 Size: 552 7487 PciI A'CATG_T Cuts at: 6495 6495 Size: 8039 PflMI CCAn_nnn'nTGG Cuts at: 486 2103 2229 4297 486 Size: 1617 126 2068 4228 Fragments arranged by size: 4228 2068 1617 126 PfoI T'CCnGG_A Cuts at: 2801 3050 3725 2801 Size: 249 675 7115 Fragments arranged by size: 7115 675 249 PmeI GTTT'AAAC Cuts at: 5038 5038 Size: 8039 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7178 7210 7178 Size: 32 8007 PshAI GACnn'nnGTC Cuts at: 4111 4111 Size: 8039 PsiI TTA'TAA Cuts at: 1184 1219 1724 1184 Size: 35 505 7499 Fragments arranged by size: 7499 505 35 PspOMI G'GGCC_C Cuts at: 993 1408 993 Size: 415 7624 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2974 3006 2974 Size: 32 8007 PstI C_TGCA'G Cuts at: 935 2410 3610 3857 4704 5086 5771 935 Size: 1475 1200 247 847 382 685 3203 Fragments arranged by size: 3203 1475 1200 847 685 382 247 PvuI CG_AT'CG Cuts at: 104 104 Size: 8039 PvuII CAG'CTG Cuts at: 504 1206 1347 3513 5464 5824 504 Size: 702 141 2166 1951 360 2719 Fragments arranged by size: 2719 2166 1951 702 360 141 RsrII CG'GwC_CG Cuts at: 6234 6234 Size: 8039 SacI G_AGCT'C Cuts at: 115 142 5053 115 Size: 27 4911 3101 Fragments arranged by size: 4911 3101 27 SalI G'TCGA_C Cuts at: 1645 5076 1645 Size: 3431 4608 SapI GCTCTTCn'nnn_ Cuts at: 1028 3664 6062 6272 1028 Size: 2636 2398 210 2795 Fragments arranged by size: 2795 2636 2398 210 SbfI CC_TGCA'GG Cuts at: 5086 5086 Size: 8039 ScaI AGT'ACT Cuts at: 1108 2917 7556 1108 Size: 1809 4639 1591 Fragments arranged by size: 4639 1809 1591 SexAI A'CCwGG_T Cuts at: 761 3485 761 Size: 2724 5315 SfcI C'TryA_G Cuts at: 33 719 931 2406 2977 3606 3853 4032 4700 Size: 686 212 1475 571 629 247 179 668 Cuts at: 4700 5082 5767 6760 6951 33 Size: 382 685 993 191 1121 Fragments arranged by size: 1475 1121 993 686 685 668 629 571 382 247 212 191 179 SfiI GGCCn_nnn'nGGCC Cuts at: 1416 2155 1416 Size: 739 7300 SfoI GGC'GCC Cuts at: 888 3369 5471 5719 888 Size: 2481 2102 248 3208 Fragments arranged by size: 3208 2481 2102 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 8039 SmaI CCC'GGG Cuts at: 3967 4555 5061 3967 Size: 588 506 6945 Fragments arranged by size: 6945 588 506 SmlI C'TyrA_G Cuts at: 133 2619 5194 6109 6601 6863 133 Size: 2486 2575 915 492 262 1309 Fragments arranged by size: 2575 2486 1309 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 8039 SphI G_CATG'C Cuts at: 5092 5592 5092 Size: 500 7539 StuI AGG'CCT Cuts at: 542 593 1923 542 Size: 51 1330 6658 Fragments arranged by size: 6658 1330 51 StyI C'CwwG_G Cuts at: 733 1484 1496 2144 2177 2555 3293 4894 5173 Size: 751 12 648 33 378 738 1601 279 Cuts at: 5173 5527 733 Size: 354 3245 Fragments arranged by size: 3245 1601 751 738 648 378 354 279 33 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 312 382 859 1497 1584 2440 5622 5755 6214 Size: 70 477 638 87 856 3182 133 459 Cuts at: 6214 6423 312 Size: 209 1928 Fragments arranged by size: 3182 1928 856 638 477 459 209 133 87 70 TatI w'GTAC_w Cuts at: 1106 2915 3125 4595 7554 1106 Size: 1809 210 1470 2959 1591 Fragments arranged by size: 2959 1809 1591 1470 210 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2176 2208 4662 4694 7556 7588 2176 Size: 32 2454 32 2862 32 2627 Fragments arranged by size: 2862 2627 2454 32 32 32 Tth111I GACn'n_nGTC Cuts at: 798 1736 5836 798 Size: 938 4100 3001 Fragments arranged by size: 4100 3001 938 XbaI T'CTAG_A Cuts at: 5070 5070 Size: 8039 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1707 2151 2511 3420 5534 1707 Size: 444 360 909 2114 4212 Fragments arranged by size: 4212 2114 909 444 360 XmaI C'CCGG_G Cuts at: 3965 4553 5059 3965 Size: 588 506 6945 Fragments arranged by size: 6945 588 506 XmnI GAAnn'nnTTC Cuts at: 1266 3658 4766 1266 Size: 2392 1108 4539 Fragments arranged by size: 4539 2392 1108 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AflII AleI AloI AscI BbvCI BcgI BfrBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I CspCI DraIII FseI FspAI NheI NruI NsiI PacI PmlI PpuMI PspXI SacII SanDI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI