(Linear) (Six-base) MAPSORT of: KIEE2583.seq Check: 6967 from: 1 to: 4926 KIEE2583.orf With 182 enzymes: SgfI * August 16, 2021 14:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2305 4926 Size: 2305 2621 AccI GT'mk_AC Cuts at: 0 1538 4926 Size: 1538 3388 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2383 2785 4184 4673 4926 Size: 2383 402 1399 489 253 Fragments arranged by size: 2383 1399 489 402 253 AfeI AGC'GCT Cuts at: 0 3915 4926 Size: 3915 1011 AhdI GACnn_n'nnGTC Cuts at: 0 981 4926 Size: 981 3945 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2323 4926 Size: 2323 2603 AlwNI CAG_nnn'CTG Cuts at: 0 2310 2506 4918 4926 Size: 2310 196 2412 8 Fragments arranged by size: 2412 2310 196 8 ApaI G_GGCC'C Cuts at: 0 889 1304 4926 Size: 889 415 3622 Fragments arranged by size: 3622 889 415 ApoI r'AATT_y Cuts at: 0 295 2410 2581 4926 Size: 295 2115 171 2345 Fragments arranged by size: 2345 2115 295 171 AseI AT'TA_AT Cuts at: 0 3194 4926 Size: 3194 1732 AvaI C'yCGr_G Cuts at: 0 3857 4445 4926 Size: 3857 588 481 Fragments arranged by size: 3857 588 481 AvrII C'CTAG_G Cuts at: 0 4786 4926 Size: 4786 140 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1205 1238 2922 2955 4926 Size: 1205 33 1684 33 1971 Fragments arranged by size: 1971 1684 1205 33 33 BanI G'GyrC_C Cuts at: 0 760 778 1329 3259 4926 Size: 760 18 551 1930 1667 Fragments arranged by size: 1930 1667 760 551 18 BanII G_rGCy'C Cuts at: 0 7 22 34 889 1304 1721 4926 Size: 7 15 12 855 415 417 3205 Fragments arranged by size: 3205 855 417 415 15 12 7 BbeI G_GCGC'C Cuts at: 0 782 3263 4926 Size: 782 2481 1663 Fragments arranged by size: 2481 1663 782 BbsI GAAGACnn'nnnn_ Cuts at: 0 656 2340 4078 4926 Size: 656 1684 1738 848 Fragments arranged by size: 1738 1684 848 656 BciVI GTATCCnnnnn_n' Cuts at: 0 351 1955 3262 3966 4926 Size: 351 1604 1307 704 960 Fragments arranged by size: 1604 1307 960 704 351 BclI T'GATC_A Cuts at: 0 488 3449 4926 Size: 488 2961 1477 Fragments arranged by size: 2961 1477 488 BglI GCCn_nnn'nGGC Cuts at: 0 1308 2047 4926 Size: 1308 739 2879 Fragments arranged by size: 2879 1308 739 BglII A'GATC_T Cuts at: 0 1286 2573 2823 4822 4926 Size: 1286 1287 250 1999 104 Fragments arranged by size: 1999 1287 1286 250 104 BlpI GC'TnA_GC Cuts at: 0 1240 3770 4604 4926 Size: 1240 2530 834 322 Fragments arranged by size: 2530 1240 834 322 Bme1580I G_kGCm'C Cuts at: 0 860 889 1304 4105 4926 Size: 860 29 415 2801 821 Fragments arranged by size: 2801 860 821 415 29 BmgBI CAC'GTC Cuts at: 0 967 3989 4050 4926 Size: 967 3022 61 876 Fragments arranged by size: 3022 967 876 61 BmrI ACTGGGnnnn_n' Cuts at: 0 853 1494 1636 4108 4926 Size: 853 641 142 2472 818 Fragments arranged by size: 2472 853 818 641 142 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2770 3114 4926 Size: 2770 344 1812 Fragments arranged by size: 2770 1812 344 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 421 1214 1624 1747 1841 2253 2677 3257 Size: 421 793 410 123 94 412 424 580 Cuts at: 3257 4926 Size: 1669 Fragments arranged by size: 1669 793 580 424 421 412 410 123 94 Bpu10I CC'TnA_GC Cuts at: 0 3556 4926 Size: 3556 1370 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 10 2496 4926 Size: 10 2486 2430 Fragments arranged by size: 2486 2430 10 BsaI GGTCTCn'nnnn_ Cuts at: 0 874 4926 Size: 874 4052 BsaAI yAC'GTr Cuts at: 0 558 2982 4926 Size: 558 2424 1944 Fragments arranged by size: 2424 1944 558 BsaBI GATnn'nnATC Cuts at: 0 2707 3004 4752 4926 Size: 2707 297 1748 174 Fragments arranged by size: 2707 1748 297 174 BsaHI Gr'CG_yC Cuts at: 0 779 3260 4926 Size: 779 2481 1666 Fragments arranged by size: 2481 1666 779 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 568 598 2068 2098 4926 Size: 568 30 1470 30 2828 Fragments arranged by size: 2828 1470 568 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 573 1621 1757 2542 3103 3241 3580 3607 Size: 573 1048 136 785 561 138 339 27 Cuts at: 3607 4926 Size: 1319 Fragments arranged by size: 1319 1048 785 573 561 339 138 136 27 BseYI C'CCAG_C Cuts at: 0 190 4926 Size: 190 4736 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3625 4926 Size: 3625 1301 BsiEI CG_ry'CG Cuts at: 0 3495 4926 Size: 3495 1431 BsiHKAI G_wGCw'C Cuts at: 0 7 34 722 2540 3047 3614 4926 Size: 7 27 688 1818 507 567 1312 Fragments arranged by size: 1818 1312 688 567 507 27 7 BsmI GAATG_Cn' Cuts at: 0 1497 3807 4926 Size: 1497 2310 1119 Fragments arranged by size: 2310 1497 1119 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3490 4926 Size: 3490 1436 Bsp1286I G_dGCh'C Cuts at: 0 7 22 34 722 860 889 1304 1721 Size: 7 15 12 688 138 29 415 417 Cuts at: 1721 2540 3047 3614 4105 4926 Size: 819 507 567 491 821 Fragments arranged by size: 821 819 688 567 507 491 417 415 138 29 15 12 7 BspHI T'CATG_A Cuts at: 0 2846 3047 3420 4926 Size: 2846 201 373 1506 Fragments arranged by size: 2846 1506 373 201 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 926 2254 2305 2648 3332 4926 Size: 926 1328 51 343 684 1594 Fragments arranged by size: 1594 1328 926 684 343 51 BsrDI GCAATG_nn' Cuts at: 0 2285 4534 4926 Size: 2285 2249 392 Fragments arranged by size: 2285 2249 392 BssSI C'ACGA_G Cuts at: 0 862 871 1720 1954 2077 2827 4926 Size: 862 9 849 234 123 750 2099 Fragments arranged by size: 2099 862 849 750 234 123 9 BstAPI GCAn_nnn'nTGC Cuts at: 0 855 1509 3603 4926 Size: 855 654 2094 1323 Fragments arranged by size: 2094 1323 855 654 BstXI CCAn_nnnn'nTGG Cuts at: 0 909 942 2043 4926 Size: 909 33 1101 2883 Fragments arranged by size: 2883 1101 909 33 BstYI r'GATC_y Cuts at: 0 518 1286 2573 2750 2823 4822 4926 Size: 518 768 1287 177 73 1999 104 Fragments arranged by size: 1999 1287 768 518 177 104 73 Bsu36I CC'TnA_GG Cuts at: 0 1033 4926 Size: 1033 3893 BtgI C'CryG_G Cuts at: 0 625 1376 2069 2261 3185 4926 Size: 625 751 693 192 924 1741 Fragments arranged by size: 1741 924 751 693 625 192 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1522 4926 Size: 1522 3404 BtsI GCAGTG_nn' Cuts at: 0 364 1979 2006 3604 4601 4926 Size: 364 1615 27 1598 997 325 Fragments arranged by size: 1615 1598 997 364 325 27 ClaI AT'CG_AT Cuts at: 0 3936 4926 Size: 3936 990 DraI TTT'AAA Cuts at: 0 4259 4926 Size: 4259 667 DrdI GACnn_nn'nnGTC Cuts at: 0 3430 4926 Size: 3430 1496 EaeI y'GGCC_r Cuts at: 0 144 1309 1379 2033 2145 2258 2683 2831 Size: 144 1165 70 654 112 113 425 148 Cuts at: 2831 2895 3492 4926 Size: 64 597 1434 Fragments arranged by size: 1434 1165 654 597 425 148 144 113 112 70 64 EagI C'GGCC_G Cuts at: 0 3492 4926 Size: 3492 1434 EarI CTCTTCn'nnn_ Cuts at: 0 197 920 1392 1892 2018 2471 3556 3950 Size: 197 723 472 500 126 453 1085 394 Cuts at: 3950 4034 4160 4926 Size: 84 126 766 Fragments arranged by size: 1085 766 723 500 472 453 394 197 126 126 84 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 323 1028 1673 4926 Size: 323 705 645 3253 Fragments arranged by size: 3253 705 645 323 EcoICRI GAG'CTC Cuts at: 0 5 32 4926 Size: 5 27 4894 Fragments arranged by size: 4894 27 5 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 421 1214 1624 1747 1841 2253 2383 2677 Size: 421 793 410 123 94 412 130 294 Cuts at: 2677 2785 3257 4184 4673 4926 Size: 108 472 927 489 253 Fragments arranged by size: 927 793 489 472 421 412 410 294 253 130 123 108 94 EcoNI CCTnn'n_nnAGG Cuts at: 0 490 3844 4926 Size: 490 3354 1082 Fragments arranged by size: 3354 1082 490 EcoO109I rG'GnC_Cy Cuts at: 0 512 885 886 1301 2040 4926 Size: 512 373 1 415 739 2886 Fragments arranged by size: 2886 739 512 415 373 1 EcoRI G'AATT_C Cuts at: 0 295 2581 4926 Size: 295 2286 2345 Fragments arranged by size: 2345 2286 295 EcoRV GAT'ATC Cuts at: 0 2919 3400 3940 4926 Size: 2919 481 540 986 Fragments arranged by size: 2919 986 540 481 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2815 3743 4926 Size: 2815 928 1183 Fragments arranged by size: 2815 1183 928 FspI TGC'GCA Cuts at: 0 992 4926 Size: 992 3934 HaeII r_GCGC'y Cuts at: 0 782 3263 3917 4926 Size: 782 2481 654 1009 Fragments arranged by size: 2481 1009 782 654 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 226 258 568 600 967 999 2095 2127 Size: 226 32 310 32 367 32 1096 32 Cuts at: 2127 2416 2448 2494 2526 3533 3565 3625 3657 Size: 289 32 46 32 1007 32 60 32 Cuts at: 3657 3961 3993 4926 Size: 304 32 933 Fragments arranged by size: 1096 1007 933 367 310 304 289 226 60 46 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1539 2961 3756 4473 4926 Size: 1539 1422 795 717 453 Fragments arranged by size: 1539 1422 795 717 453 HindIII A'AGCT_T Cuts at: 0 4431 4904 4926 Size: 4431 473 22 Fragments arranged by size: 4431 473 22 HpaI GTT'AAC Cuts at: 0 4473 4926 Size: 4473 453 KasI G'GCGC_C Cuts at: 0 778 3259 4926 Size: 778 2481 1667 Fragments arranged by size: 2481 1667 778 MfeI C'AATT_G Cuts at: 0 4510 4926 Size: 4510 416 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 639 719 3398 3683 4565 4926 Size: 639 80 2679 285 882 361 Fragments arranged by size: 2679 882 639 361 285 80 MscI TGG'CCA Cuts at: 0 146 1381 2035 2833 4926 Size: 146 1235 654 798 2093 Fragments arranged by size: 2093 1235 798 654 146 MslI CAynn'nnrTG Cuts at: 0 940 1183 1912 2665 2845 3478 4926 Size: 940 243 729 753 180 633 1448 Fragments arranged by size: 1448 940 753 729 633 243 180 MspA1I CmG'CkG Cuts at: 0 396 962 1098 1239 1754 3405 3491 3497 Size: 396 566 136 141 515 1651 86 6 Cuts at: 3497 4926 Size: 1429 Fragments arranged by size: 1651 1429 566 515 396 141 136 86 6 NarI GG'CG_CC Cuts at: 0 779 3260 4926 Size: 779 2481 1666 Fragments arranged by size: 2481 1666 779 NcoI C'CATG_G Cuts at: 0 625 1376 2069 3185 4926 Size: 625 751 693 1116 1741 Fragments arranged by size: 1741 1116 751 693 625 NdeI CA'TA_TG Cuts at: 0 2421 4926 Size: 2421 2505 NotI GC'GGCC_GC Cuts at: 0 3492 4926 Size: 3492 1434 NspI r_CATG'y Cuts at: 0 1578 3015 3132 4382 4834 4926 Size: 1578 1437 117 1250 452 92 Fragments arranged by size: 1578 1437 1250 452 117 92 PasI CC'CwG_GG Cuts at: 0 765 1317 4926 Size: 765 552 3609 Fragments arranged by size: 3609 765 552 PflMI CCAn_nnn'nTGG Cuts at: 0 378 1995 2121 4189 4926 Size: 378 1617 126 2068 737 Fragments arranged by size: 2068 1617 737 378 126 PfoI T'CCnGG_A Cuts at: 0 2693 2942 3617 4926 Size: 2693 249 675 1309 Fragments arranged by size: 2693 1309 675 249 PshAI GACnn'nnGTC Cuts at: 0 4003 4926 Size: 4003 923 PsiI TTA'TAA Cuts at: 0 1076 1111 1616 4926 Size: 1076 35 505 3310 Fragments arranged by size: 3310 1076 505 35 PspOMI G'GGCC_C Cuts at: 0 885 1300 4926 Size: 885 415 3626 Fragments arranged by size: 3626 885 415 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2866 2898 4926 Size: 2866 32 2028 Fragments arranged by size: 2866 2028 32 PstI C_TGCA'G Cuts at: 0 827 2302 3502 3749 4596 4926 Size: 827 1475 1200 247 847 330 Fragments arranged by size: 1475 1200 847 827 330 247 PvuII CAG'CTG Cuts at: 0 396 1098 1239 3405 4926 Size: 396 702 141 2166 1521 Fragments arranged by size: 2166 1521 702 396 141 SacI G_AGCT'C Cuts at: 0 7 34 4926 Size: 7 27 4892 Fragments arranged by size: 4892 27 7 SalI G'TCGA_C Cuts at: 0 1537 4926 Size: 1537 3389 SapI GCTCTTCn'nnn_ Cuts at: 0 920 3556 4926 Size: 920 2636 1370 Fragments arranged by size: 2636 1370 920 ScaI AGT'ACT Cuts at: 0 1000 2809 4926 Size: 1000 1809 2117 Fragments arranged by size: 2117 1809 1000 SexAI A'CCwGG_T Cuts at: 0 653 3377 4926 Size: 653 2724 1549 Fragments arranged by size: 2724 1549 653 SfcI C'TryA_G Cuts at: 0 611 823 2298 2869 3498 3745 3924 4592 Size: 611 212 1475 571 629 247 179 668 Cuts at: 4592 4926 Size: 334 Fragments arranged by size: 1475 668 629 611 571 334 247 212 179 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1308 2047 4926 Size: 1308 739 2879 Fragments arranged by size: 2879 1308 739 SfoI GGC'GCC Cuts at: 0 780 3261 4926 Size: 780 2481 1665 Fragments arranged by size: 2481 1665 780 SmaI CCC'GGG Cuts at: 0 3859 4447 4926 Size: 3859 588 479 Fragments arranged by size: 3859 588 479 SmlI C'TyrA_G Cuts at: 0 25 2511 4926 Size: 25 2486 2415 Fragments arranged by size: 2486 2415 25 StuI AGG'CCT Cuts at: 0 434 485 1815 4926 Size: 434 51 1330 3111 Fragments arranged by size: 3111 1330 434 51 StyI C'CwwG_G Cuts at: 0 625 1376 1388 2036 2069 2447 3185 4786 Size: 625 751 12 648 33 378 738 1601 Cuts at: 4786 4926 Size: 140 Fragments arranged by size: 1601 751 738 648 625 378 140 33 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 204 274 751 1389 1476 2332 4926 Size: 204 70 477 638 87 856 2594 Fragments arranged by size: 2594 856 638 477 204 87 70 TatI w'GTAC_w Cuts at: 0 998 2807 3017 4487 4926 Size: 998 1809 210 1470 439 Fragments arranged by size: 1809 1470 998 439 210 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2068 2100 4554 4586 4926 Size: 2068 32 2454 32 340 Fragments arranged by size: 2454 2068 340 32 32 Tth111I GACn'n_nGTC Cuts at: 0 690 1628 4926 Size: 690 938 3298 Fragments arranged by size: 3298 938 690 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1599 2043 2403 3312 4926 Size: 1599 444 360 909 1614 Fragments arranged by size: 1614 1599 909 444 360 XmaI C'CCGG_G Cuts at: 0 3857 4445 4926 Size: 3857 588 481 Fragments arranged by size: 3857 588 481 XmnI GAAnn'nnTTC Cuts at: 0 1158 3550 4658 4926 Size: 1158 2392 1108 268 Fragments arranged by size: 2392 1158 1108 268 Enzymes that do cut: AarI AccI AcuI AfeI AhdI AlfI AlwNI ApaI ApoI AseI AvaI AvrII BaeI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NdeI NotI NspI PasI PflMI PfoI PshAI PsiI PspOMI PsrI PstI PvuII SacI SalI SapI ScaI SexAI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AleI AloI ApaLI AscI AsiSI BamHI BbvCI BcgI BfrBI BmtI BsaWI BsiWI BspEI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstZ17I CspCI DraIII FseI FspAI KpnI MluI NaeI NgoMIV NheI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PspXI PvuI RsrII SacII SanDI SbfI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI ZraI