(Linear) (Six-base) MAPSORT of: KIEE2673.seq Check: 6339 from: 1 to: 2379 KIEE2673.orf With 182 enzymes: SgfI * February 10, 2017 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1777 2379 Size: 1777 602 AccI GT'mk_AC Cuts at: 0 1220 1549 2379 Size: 1220 329 830 Fragments arranged by size: 1220 830 329 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 380 2379 Size: 121 259 1999 Fragments arranged by size: 1999 259 121 AlwNI CAG_nnn'CTG Cuts at: 0 990 2379 Size: 990 1389 ApoI r'AATT_y Cuts at: 0 443 518 757 831 915 1156 1386 1615 Size: 443 75 239 74 84 241 230 229 Cuts at: 1615 2163 2308 2379 Size: 548 145 71 Fragments arranged by size: 548 443 241 239 230 229 145 84 75 74 71 AvaI C'yCGr_G Cuts at: 0 18 33 1988 2379 Size: 18 15 1955 391 Fragments arranged by size: 1955 391 18 15 BamHI G'GATC_C Cuts at: 0 693 2379 Size: 693 1686 BanI G'GyrC_C Cuts at: 0 37 1765 2379 Size: 37 1728 614 Fragments arranged by size: 1728 614 37 BbeI G_GCGC'C Cuts at: 0 41 2379 Size: 41 2338 BbsI GAAGACnn'nnnn_ Cuts at: 0 1349 1973 2379 Size: 1349 624 406 Fragments arranged by size: 1349 624 406 BfrBI ATG'CAT Cuts at: 0 1471 2379 Size: 1471 908 BglII A'GATC_T Cuts at: 0 2298 2379 Size: 2298 81 BlpI GC'TnA_GC Cuts at: 0 2005 2379 Size: 2005 374 BmrI ACTGGGnnnn_n' Cuts at: 0 963 2379 Size: 963 1416 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 981 2379 Size: 981 1398 Bpu10I CC'TnA_GC Cuts at: 0 806 1800 2379 Size: 806 994 579 Fragments arranged by size: 994 806 579 BsaBI GATnn'nnATC Cuts at: 0 647 2379 Size: 647 1732 BsaHI Gr'CG_yC Cuts at: 0 38 2379 Size: 38 2341 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2019 2049 2379 Size: 2019 30 330 Fragments arranged by size: 2019 330 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 33 36 39 139 703 2379 Size: 33 3 3 100 564 1676 Fragments arranged by size: 1676 564 100 33 3 3 BsiHKAI G_wGCw'C Cuts at: 0 25 2379 Size: 25 2354 BsmI GAATG_Cn' Cuts at: 0 586 624 1471 2379 Size: 586 38 847 908 Fragments arranged by size: 908 847 586 38 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1540 2379 Size: 1540 839 Bsp1286I G_dGCh'C Cuts at: 0 25 2379 Size: 25 2354 BspHI T'CATG_A Cuts at: 0 1382 1440 1962 2379 Size: 1382 58 522 417 Fragments arranged by size: 1382 522 417 58 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1777 2379 Size: 1777 602 BsrDI GCAATG_nn' Cuts at: 0 796 1746 1934 2379 Size: 796 950 188 445 Fragments arranged by size: 950 796 445 188 BssSI C'ACGA_G Cuts at: 0 836 2379 Size: 836 1543 BstAPI GCAn_nnn'nTGC Cuts at: 0 1408 2379 Size: 1408 971 BstEII G'GTnAC_C Cuts at: 0 1021 2379 Size: 1021 1358 BstXI CCAn_nnnn'nTGG Cuts at: 0 571 1920 2379 Size: 571 1349 459 Fragments arranged by size: 1349 571 459 BstYI r'GATC_y Cuts at: 0 693 2298 2379 Size: 693 1605 81 Fragments arranged by size: 1605 693 81 BstZ17I GTA'TAC Cuts at: 0 1550 2379 Size: 1550 829 ClaI AT'CG_AT Cuts at: 0 651 2379 Size: 651 1728 DraI TTT'AAA Cuts at: 0 731 2150 2379 Size: 731 1419 229 Fragments arranged by size: 1419 731 229 DraIII CAC_nnn'GTG Cuts at: 0 590 2379 Size: 590 1789 EaeI y'GGCC_r Cuts at: 0 659 1081 2379 Size: 659 422 1298 Fragments arranged by size: 1298 659 422 EarI CTCTTCn'nnn_ Cuts at: 0 78 2170 2379 Size: 78 2092 209 Fragments arranged by size: 2092 209 78 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 380 2379 Size: 121 259 1999 Fragments arranged by size: 1999 259 121 EcoRI G'AATT_C Cuts at: 0 443 757 831 1386 2379 Size: 443 314 74 555 993 Fragments arranged by size: 993 555 443 314 74 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 168 2379 Size: 168 2211 HaeII r_GCGC'y Cuts at: 0 41 100 2379 Size: 41 59 2279 Fragments arranged by size: 2279 59 41 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1944 1976 2094 2126 2379 Size: 1944 32 118 32 253 Fragments arranged by size: 1944 253 118 32 32 HincII GTy'rAC Cuts at: 0 683 2379 Size: 683 1696 HindIII A'AGCT_T Cuts at: 0 145 1941 2379 Size: 145 1796 438 Fragments arranged by size: 1796 438 145 HpaI GTT'AAC Cuts at: 0 683 2379 Size: 683 1696 KasI G'GCGC_C Cuts at: 0 37 2379 Size: 37 2342 MfeI C'AATT_G Cuts at: 0 1655 2379 Size: 1655 724 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1319 2379 Size: 1319 1060 MscI TGG'CCA Cuts at: 0 661 1083 2379 Size: 661 422 1296 Fragments arranged by size: 1296 661 422 NarI GG'CG_CC Cuts at: 0 38 2379 Size: 38 2341 NsiI A_TGCA'T Cuts at: 0 1473 2379 Size: 1473 906 NspI r_CATG'y Cuts at: 0 1810 2379 Size: 1810 569 PshAI GACnn'nnGTC Cuts at: 0 1014 2379 Size: 1014 1365 PspXI vC'TCGA_Gb Cuts at: 0 1988 2379 Size: 1988 391 PstI C_TGCA'G Cuts at: 0 302 333 1853 2379 Size: 302 31 1520 526 Fragments arranged by size: 1520 526 302 31 SfcI C'TryA_G Cuts at: 0 298 329 422 1849 2379 Size: 298 31 93 1427 530 Fragments arranged by size: 1427 530 298 93 31 SfoI GGC'GCC Cuts at: 0 39 2379 Size: 39 2340 SmlI C'TyrA_G Cuts at: 0 1988 2379 Size: 1988 391 TatI w'GTAC_w Cuts at: 0 2254 2379 Size: 2254 125 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 822 854 2379 Size: 822 32 1525 Fragments arranged by size: 1525 822 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1563 1759 1920 2379 Size: 1563 196 161 459 Fragments arranged by size: 1563 459 196 161 XhoI C'TCGA_G Cuts at: 0 1988 2379 Size: 1988 391 XmnI GAAnn'nnTTC Cuts at: 0 148 443 851 2379 Size: 148 295 408 1528 Fragments arranged by size: 1528 408 295 148 Enzymes that do cut: AarI AccI AcuI AlwNI ApoI AvaI BamHI BanI BbeI BbsI BfrBI BglII BlpI BmrI BplI Bpu10I BsaBI BsaHI BsaXI BseRI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BssSI BstAPI BstEII BstXI BstYI BstZ17I ClaI DraI DraIII EaeI EarI Eco57MI EcoRI FalI HaeII Hin4I HincII HindIII HpaI KasI MfeI MmeI MscI NarI NsiI NspI PshAI PspXI PstI SfcI SfoI SmlI TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanII BbvCI BcgI BciVI BclI BglI Bme1580I BmgBI BmtI BpmI BpuEI BsaI BsaAI BsaWI BseYI BsgI BsiEI BsiWI BspEI BsrBI BsrFI BsrGI BssHII BstBI Bsu36I BtgI BtgZI BtsI CspCI DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI KpnI MluI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII Tth111I XbaI XmaI ZraI