(Circular) (Six-base) MAPSORT of: pF1KE2615.seq Check: 7423 from: 1 to: 4175 pF1KE2615 4175 bp With 182 enzymes: SgfI * April 1, 2016 14:38 .. AccI GT'mk_AC Cuts at: 1117 1213 1117 Size: 96 4079 Acc65I G'GTAC_C Cuts at: 1191 1191 Size: 4175 AclI AA'CG_TT Cuts at: 4015 4015 Size: 4175 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 176 964 1117 2000 2432 3179 3447 176 Size: 788 153 883 432 747 268 904 Fragments arranged by size: 904 883 788 747 432 268 153 AfeI AGC'GCT Cuts at: 532 1000 532 Size: 468 3707 AflIII A'CryG_T Cuts at: 698 1527 2631 3608 698 Size: 829 1104 977 1265 Fragments arranged by size: 1265 1104 977 829 AgeI A'CCGG_T Cuts at: 2563 2563 Size: 4175 AlwNI CAG_nnn'CTG Cuts at: 196 713 1480 3047 196 Size: 517 767 1567 1324 Fragments arranged by size: 1567 1324 767 517 ApaLI G'TGCA_C Cuts at: 2945 2945 Size: 4175 ApoI r'AATT_y Cuts at: 588 1179 3528 3784 588 Size: 591 2349 256 979 Fragments arranged by size: 2349 979 591 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4175 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4175 AvaI C'yCGr_G Cuts at: 1195 1195 Size: 4175 BamHI G'GATC_C Cuts at: 1200 1200 Size: 4175 BanI G'GyrC_C Cuts at: 451 642 1191 1605 1853 1888 451 Size: 191 549 414 248 35 2738 Fragments arranged by size: 2738 549 414 248 191 35 BanII G_rGCy'C Cuts at: 1083 1189 2219 1083 Size: 106 1030 3039 Fragments arranged by size: 3039 1030 106 BbeI G_GCGC'C Cuts at: 1609 1857 1609 Size: 248 3927 BbsI GAAGACnn'nnnn_ Cuts at: 164 770 164 Size: 606 3569 BbvCI CC'TCA_GC Cuts at: 563 563 Size: 4175 BciVI GTATCCnnnnn_n' Cuts at: 2073 2834 4175 2073 Size: 761 1341 2073 Fragments arranged by size: 2073 1341 761 BclI T'GATC_A Cuts at: 1695 1695 Size: 4175 BglII A'GATC_T Cuts at: 1690 3727 1690 Size: 2037 2138 BlpI GC'TnA_GC Cuts at: 1287 1287 Size: 4175 Bme1580I G_kGCm'C Cuts at: 647 1800 1893 2949 647 Size: 1153 93 1056 1873 Fragments arranged by size: 1873 1153 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 793 1387 1468 1803 3947 793 Size: 594 81 335 2144 1021 Fragments arranged by size: 2144 1021 594 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 552 684 1097 2542 552 Size: 132 413 1445 2185 Fragments arranged by size: 2185 1445 413 132 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 753 1071 753 Size: 318 3857 Bpu10I CC'TnA_GC Cuts at: 563 563 Size: 4175 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1024 1351 2230 2722 3020 1024 Size: 327 879 492 298 2179 Fragments arranged by size: 2179 879 492 327 298 BsaI GGTCTCn'nnnn_ Cuts at: 824 3879 824 Size: 3055 1120 BsaAI yAC'GTr Cuts at: 365 1528 365 Size: 1163 3012 BsaBI GATnn'nnATC Cuts at: 6 1689 3726 6 Size: 1683 2037 455 Fragments arranged by size: 2037 1683 455 BsaHI Gr'CG_yC Cuts at: 1606 1854 2556 4052 1606 Size: 248 702 1496 1729 Fragments arranged by size: 1729 1496 702 248 BsaWI w'CCGG_w Cuts at: 731 1364 1885 2563 2837 2984 3395 731 Size: 633 521 678 274 147 411 1511 Fragments arranged by size: 1511 678 633 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 913 943 913 Size: 30 4145 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 551 921 551 Size: 370 3805 BseYI C'CCAG_C Cuts at: 938 1054 1600 2358 2935 3431 938 Size: 116 546 758 577 496 1682 Fragments arranged by size: 1682 758 577 546 496 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 200 230 439 200 Size: 30 209 3936 Fragments arranged by size: 3936 209 30 BsiEI CG_ry'CG Cuts at: 104 1717 1763 2971 104 Size: 1613 46 1208 1308 Fragments arranged by size: 1613 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 901 912 1189 1967 2157 2949 901 Size: 11 277 778 190 792 2127 Fragments arranged by size: 2127 792 778 277 190 11 BsmI GAATG_Cn' Cuts at: 191 333 191 Size: 142 4033 Bsp1286I G_dGCh'C Cuts at: 647 901 912 1083 1189 1800 1893 1967 2157 Size: 254 11 171 106 611 93 74 190 Cuts at: 2157 2219 2949 647 Size: 62 730 1873 Fragments arranged by size: 1873 730 611 254 190 171 106 93 74 62 11 BspEI T'CCGG_A Cuts at: 3395 3395 Size: 4175 BspHI T'CATG_A Cuts at: 3351 3351 Size: 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 418 1225 1439 1741 2122 418 Size: 807 214 302 381 2471 Fragments arranged by size: 2471 807 381 302 214 BsrBI CCG'CTC Cuts at: 50 2467 2521 4005 4170 50 Size: 2417 54 1484 165 55 Fragments arranged by size: 2417 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2087 2087 Size: 4175 BsrFI r'CCGG_y Cuts at: 234 833 2173 2354 2563 234 Size: 599 1340 181 209 1846 Fragments arranged by size: 1846 1340 599 209 181 BssSI C'ACGA_G Cuts at: 880 2446 2804 880 Size: 1566 358 2251 Fragments arranged by size: 2251 1566 358 BstAPI GCAn_nnn'nTGC Cuts at: 1552 1552 Size: 4175 BstBI TT'CG_AA Cuts at: 2536 2536 Size: 4175 BstYI r'GATC_y Cuts at: 1 983 1200 1667 1690 2025 2271 3283 3369 Size: 982 217 467 23 335 246 1012 86 Cuts at: 3369 3381 3727 1 Size: 12 346 449 Fragments arranged by size: 1012 982 467 449 346 335 246 217 86 23 12 Bsu36I CC'TnA_GG Cuts at: 659 659 Size: 4175 BtgI C'CryG_G Cuts at: 107 467 987 107 Size: 360 520 3295 Fragments arranged by size: 3295 520 360 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 560 2125 2306 2469 560 Size: 1565 181 163 2266 Fragments arranged by size: 2266 1565 181 163 BtsI GCAGTG_nn' Cuts at: 910 1418 910 Size: 508 3667 DraI TTT'AAA Cuts at: 1174 1174 Size: 4175 DrdI GACnn_nn'nnGTC Cuts at: 348 1881 2739 348 Size: 1533 858 1784 Fragments arranged by size: 1784 1533 858 EaeI y'GGCC_r Cuts at: 401 470 1760 1934 2325 2352 3697 401 Size: 69 1290 174 391 27 1345 879 Fragments arranged by size: 1345 1290 879 391 174 69 27 EagI C'GGCC_G Cuts at: 1760 1760 Size: 4175 EarI CTCTTCn'nnn_ Cuts at: 842 2198 2408 842 Size: 1356 210 2609 Fragments arranged by size: 2609 1356 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2383 2693 2839 3985 2383 Size: 310 146 1146 2573 Fragments arranged by size: 2573 1146 310 146 EcoICRI GAG'CTC Cuts at: 1187 1187 Size: 4175 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 176 753 964 1071 1117 2000 2432 3179 3447 Size: 577 211 107 46 883 432 747 268 Cuts at: 3447 176 Size: 904 Fragments arranged by size: 904 883 747 577 432 268 211 107 46 EcoO109I rG'GnC_Cy Cuts at: 1156 1314 1156 Size: 158 4017 EcoRI G'AATT_C Cuts at: 1179 1179 Size: 4175 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2194 2194 Size: 4175 FspI TGC'GCA Cuts at: 1956 1956 Size: 4175 HaeII r_GCGC'y Cuts at: 516 534 1002 1609 1857 2879 3858 516 Size: 18 468 607 248 1022 979 833 Fragments arranged by size: 1022 979 833 607 468 248 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2017 2047 2049 2079 2017 Size: 30 2 30 4113 Fragments arranged by size: 4113 30 30 2 HincII GTy'rAC Cuts at: 1214 1214 Size: 4175 KasI G'GCGC_C Cuts at: 1605 1853 1605 Size: 248 3927 KpnI G_GTAC'C Cuts at: 1195 1195 Size: 4175 MluI A'CGCG_T Cuts at: 3608 3608 Size: 4175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2846 3030 2846 Size: 184 3991 MscI TGG'CCA Cuts at: 403 472 1936 403 Size: 69 1464 2642 Fragments arranged by size: 2642 1464 69 MslI CAynn'nnrTG Cuts at: 2164 2291 2164 Size: 127 4048 MspA1I CmG'CkG Cuts at: 423 1286 1600 1960 2973 3218 423 Size: 863 314 360 1013 245 1380 Fragments arranged by size: 1380 1013 863 360 314 245 NaeI GCC'GGC Cuts at: 2356 2356 Size: 4175 NarI GG'CG_CC Cuts at: 1606 1854 1606 Size: 248 3927 NcoI C'CATG_G Cuts at: 107 467 107 Size: 360 3815 NgoMIV G'CCGG_C Cuts at: 2354 2354 Size: 4175 NspI r_CATG'y Cuts at: 702 1228 1728 2635 702 Size: 526 500 907 2242 Fragments arranged by size: 2242 907 526 500 PasI CC'CwG_GG Cuts at: 892 892 Size: 4175 PciI A'CATG_T Cuts at: 698 2631 698 Size: 1933 2242 PflMI CCAn_nnn'nTGG Cuts at: 961 961 Size: 4175 PmeI GTTT'AAAC Cuts at: 1174 1174 Size: 4175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3314 3346 3314 Size: 32 4143 PshAI GACnn'nnGTC Cuts at: 164 164 Size: 4175 PstI C_TGCA'G Cuts at: 1222 1907 1222 Size: 685 3490 PvuI CG_AT'CG Cuts at: 104 104 Size: 4175 PvuII CAG'CTG Cuts at: 423 1600 1960 423 Size: 1177 360 2638 Fragments arranged by size: 2638 1177 360 RsrII CG'GwC_CG Cuts at: 2370 2370 Size: 4175 SacI G_AGCT'C Cuts at: 1189 1189 Size: 4175 SalI G'TCGA_C Cuts at: 1212 1212 Size: 4175 SapI GCTCTTCn'nnn_ Cuts at: 2198 2408 2198 Size: 210 3965 SbfI CC_TGCA'GG Cuts at: 1222 1222 Size: 4175 ScaI AGT'ACT Cuts at: 3692 3692 Size: 4175 SfcI C'TryA_G Cuts at: 33 1218 1903 2896 3087 33 Size: 1185 685 993 191 1121 Fragments arranged by size: 1185 1121 993 685 191 SfoI GGC'GCC Cuts at: 1607 1855 1607 Size: 248 3927 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4175 SgrAI Cr'CCGG_yG Cuts at: 234 234 Size: 4175 SmaI CCC'GGG Cuts at: 1197 1197 Size: 4175 SmlI C'TyrA_G Cuts at: 1039 1330 2245 2737 2999 1039 Size: 291 915 492 262 2215 Fragments arranged by size: 2215 915 492 291 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4175 SphI G_CATG'C Cuts at: 1228 1728 1228 Size: 500 3675 SspI AAT'ATT Cuts at: 970 970 Size: 4175 StuI AGG'CCT Cuts at: 1030 1030 Size: 4175 StyI C'CwwG_G Cuts at: 107 467 1031 1309 1663 107 Size: 360 564 278 354 2619 Fragments arranged by size: 2619 564 360 354 278 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1758 1891 2350 2559 1758 Size: 133 459 209 3374 Fragments arranged by size: 3374 459 209 133 TatI w'GTAC_w Cuts at: 3690 3690 Size: 4175 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1084 1116 3692 3724 1084 Size: 32 2576 32 1535 Fragments arranged by size: 2576 1535 32 32 Tth111I GACn'n_nGTC Cuts at: 1972 1972 Size: 4175 XbaI T'CTAG_A Cuts at: 1206 1206 Size: 4175 XcmI CCAnnnn_n'nnnnTGG Cuts at: 257 756 1670 257 Size: 499 914 2762 Fragments arranged by size: 2762 914 499 XmaI C'CCGG_G Cuts at: 1195 1195 Size: 4175 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SnaBI SrfI SwaI XhoI XmnI ZraI