################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrGI Bst1107I BstEII BstPI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SalI SbfI SexAI SfcI SfiI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 355 GGTACC G^GTACC AscI 18 GGCGCGCC GG^CGCGCC Asp718I 355 GGTACC G^GTACC AvaI 30 CTCGGG C^[CT]CG[GA]G BbeI 48 GGCGCC GGCGC^C BciVI 586 GGATAC GTATCC(6/5) BclI 1815 TGATCA T^GATCA BglI 54 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 1766 ACCGGA [AT]^CCGG[AT] BsiEI 107 CGGCCG CG[GA][CT]^CG BsmBI 1081 GAGACG CGTCTC(1/5) BsoBI 30 CTCGGG C^[CT]CG[GA]G BspHI 707 TCATGA T^CATGA BsrBI 1387 CCGCTC CCGCTC(-3/-3) BsrDI 1474 GCAATG GCAATG(2/0) BsrFI 200 ACCGGC [GA]^CCGG[CT] BssHII 19 GCGCGC G^CGCGC BstAPI 1507 GCATCCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 451 TTCGAA TT^CGAA Cfr10I 200 ACCGGC [GA]^CCGG[CT] DraII 409 AGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 1844 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 107 CGGCCG C^GGCCG EclXI 107 CGGCCG C^GGCCG Eco52I 107 CGGCCG C^GGCCG EcoO109I 409 AGGGCCT [GA]G^G[ACGT]CC[CT] FbaI 1815 TGATCA T^GATCA HincII 1684 GTTGAC GT[CT]^[GA]AC HindII 1684 GTTGAC GT[CT]^[GA]AC KasI 48 GGCGCC G^GCGCC KpnI 355 GGTACC GGTAC^C MslI 1446 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 48 GGCGCC GG^CGCC NotI 106 GCGGCCGC GC^GGCCGC NspI 822 GCATGC [GA]CATG^[CT] NspV 451 TTCGAA TT^CGAA PvuII 390 CAGCTG CAG^CTG RcaI 707 TCATGA T^CATGA SacI 166 GAGCTC GAGCT^C ScaI 1667 AGTACT AGT^ACT SfoI 48 GGCGCC GGC^GCC SfuI 451 TTCGAA TT^CGAA SmlI 2028 CTCAAG C^T[CT][GA]AG SphI 822 GCATGC GCATG^C StuI 1461 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 736 AAATTT [GA]^AATT[CT] 1434 GAATTT AcyI 48 GGCGCC G[GA]^CG[CT]C 1621 GGCGTC AocI 345 CCTGAGG CC^T[ACGT]AGG 1464 CCTCAGG ApoI 736 AAATTT [GA]^AATT[CT] 1434 GAATTT AspHI 166 GAGCTC G[AT]GC[AT]^C 1110 GAGCAC AvaII 1644 GGACC G^G[AT]CC 1852 GGTCC BalI 311 TGGCCA TGG^CCA 1490 TGGCCA BbsI 403 GAAGAC GAAGAC(2/6) 1951 GAAGAC BpuAI 403 GAAGAC GAAGAC(2/6) 1951 GAAGAC BsaHI 48 GGCGCC G[GA]^CG[CT]C 1621 GGCGTC BsiHKAI 166 GAGCTC G[AT]GC[AT]^C 1110 GAGCAC BssSI 298 CACGAG CACGAG(-5/-1) 2049 CACGAG BstXI 1355 CCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2048 CCACGAGTCTGG BstYI 625 GGATCT [GA]^GATC[CT] 834 GGATCT Bsu36I 345 CCTGAGG CC^T[ACGT]AGG 1464 CCTCAGG Eco81I 345 CCTGAGG CC^T[ACGT]AGG 1464 CCTCAGG EcoT14I 158 CCATGG C^C[AT][AT]GG 1007 CCATGG Hin1I 48 GGCGCC G[GA]^CG[CT]C 1621 GGCGTC KspI 58 CCGCGG CCGC^GG 110 CCGCGG MflI 625 GGATCT [GA]^GATC[CT] 834 GGATCT MluNI 311 TGGCCA TGG^CCA 1490 TGGCCA MscI 311 TGGCCA TGG^CCA 1490 TGGCCA NcoI 158 CCATGG C^CATGG 1007 CCATGG SacII 58 CCGCGG CCGC^GG 110 CCGCGG SapI 2076 GAAGAGC GCTCTTC(1/4) 267 GCTCTTC StyI 158 CCATGG C^C[AT][AT]GG 1007 CCATGG XhoII 625 GGATCT [GA]^GATC[CT] 834 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I