(Circular) (Six-base) MAPSORT of: pF1KE2692.seq Check: 8166 from: 1 to: 5291 pF1KE2692 5291 bp With 182 enzymes: SgfI * June 23, 2017 13:27 .. AccI GT'mk_AC Cuts at: 2329 2329 Size: 5291 Acc65I G'GTAC_C Cuts at: 463 2307 463 Size: 1844 3447 AclI AA'CG_TT Cuts at: 5131 5131 Size: 5291 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 507 1764 1957 3116 3548 4295 4563 507 Size: 1257 193 1159 432 747 268 1235 Fragments arranged by size: 1257 1235 1159 747 432 268 193 AflIII A'CryG_T Cuts at: 2643 3747 4724 2643 Size: 1104 977 3210 Fragments arranged by size: 3210 1104 977 AgeI A'CCGG_T Cuts at: 3679 3679 Size: 5291 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1257 1257 Size: 5291 AlwNI CAG_nnn'CTG Cuts at: 904 1861 2122 2596 4163 904 Size: 957 261 474 1567 2032 Fragments arranged by size: 2032 1567 957 474 261 ApaLI G'TGCA_C Cuts at: 4061 4061 Size: 5291 ApoI r'AATT_y Cuts at: 844 1542 2295 4644 4900 844 Size: 698 753 2349 256 1235 Fragments arranged by size: 2349 1235 753 698 256 AscI GG'CGCG_CC Cuts at: 127 127 Size: 5291 AseI AT'TA_AT Cuts at: 20 20 Size: 5291 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5291 AvaI C'yCGr_G Cuts at: 138 2311 138 Size: 2173 3118 BamHI G'GATC_C Cuts at: 2316 2316 Size: 5291 BanI G'GyrC_C Cuts at: 156 463 2251 2307 2721 2969 3004 156 Size: 307 1788 56 414 248 35 2443 Fragments arranged by size: 2443 1788 414 307 248 56 35 BanII G_rGCy'C Cuts at: 278 437 1742 2305 3335 278 Size: 159 1305 563 1030 2234 Fragments arranged by size: 2234 1305 1030 563 159 BbeI G_GCGC'C Cuts at: 160 2725 2973 160 Size: 2565 248 2478 Fragments arranged by size: 2565 2478 248 BbsI GAAGACnn'nnnn_ Cuts at: 518 2066 518 Size: 1548 3743 BciVI GTATCCnnnnn_n' Cuts at: 688 3189 3950 5291 688 Size: 2501 761 1341 688 Fragments arranged by size: 2501 1341 761 688 BclI T'GATC_A Cuts at: 1923 2811 1923 Size: 888 4403 BglI GCCn_nnn'nGGC Cuts at: 168 168 Size: 5291 BglII A'GATC_T Cuts at: 2806 4843 2806 Size: 2037 3254 BlpI GC'TnA_GC Cuts at: 2403 2403 Size: 5291 Bme1580I G_kGCm'C Cuts at: 2254 2916 3009 4065 2254 Size: 662 93 1056 3480 Fragments arranged by size: 3480 1056 662 93 BmgBI CAC'GTC Cuts at: 939 939 Size: 5291 BmrI ACTGGGnnnn_n' Cuts at: 892 2503 2584 2919 5063 892 Size: 1611 81 335 2144 1120 Fragments arranged by size: 2144 1611 1120 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1758 2079 3658 1758 Size: 321 1579 3391 Fragments arranged by size: 3391 1579 321 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1207 1236 2185 2227 1207 Size: 29 949 42 4271 Fragments arranged by size: 4271 949 42 29 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2121 2467 3346 3838 4136 2121 Size: 346 879 492 298 3276 Fragments arranged by size: 3276 879 492 346 298 BsaI GGTCTCn'nnnn_ Cuts at: 403 2207 2222 4995 403 Size: 1804 15 2773 699 Fragments arranged by size: 2773 1804 699 15 BsaAI yAC'GTr Cuts at: 2644 2644 Size: 5291 BsaBI GATnn'nnATC Cuts at: 6 2805 4842 6 Size: 2799 2037 455 Fragments arranged by size: 2799 2037 455 BsaHI Gr'CG_yC Cuts at: 157 1730 2722 2970 3672 5168 157 Size: 1573 992 248 702 1496 280 Fragments arranged by size: 1573 1496 992 702 280 248 BsaWI w'CCGG_w Cuts at: 1874 2480 3001 3679 3953 4100 4511 1874 Size: 606 521 678 274 147 411 2654 Fragments arranged by size: 2654 678 606 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 956 986 1442 1472 1483 1513 2137 2167 956 Size: 30 456 30 11 30 624 30 4080 Fragments arranged by size: 4080 624 456 30 30 30 30 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 222 225 286 313 1700 1750 2124 222 Size: 3 61 27 1387 50 374 3389 Fragments arranged by size: 3389 1387 374 61 50 27 3 BseYI C'CCAG_C Cuts at: 192 2716 3474 4051 4547 192 Size: 2524 758 577 496 936 Fragments arranged by size: 2524 936 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 388 486 1153 388 Size: 98 667 4526 Fragments arranged by size: 4526 667 98 BsiEI CG_ry'CG Cuts at: 104 218 2833 2879 4087 104 Size: 114 2615 46 1208 1308 Fragments arranged by size: 2615 1308 1208 114 46 BsiHKAI G_wGCw'C Cuts at: 278 1222 2305 3083 3273 4065 278 Size: 944 1083 778 190 792 1504 Fragments arranged by size: 1504 1083 944 792 778 190 BsmBI CGTCTCn'nnnn_ Cuts at: 1183 1183 Size: 5291 Bsp1286I G_dGCh'C Cuts at: 278 437 1222 1742 2254 2305 2916 3009 3083 Size: 159 785 520 512 51 611 93 74 Cuts at: 3083 3273 3335 4065 278 Size: 190 62 730 1504 Fragments arranged by size: 1504 785 730 611 520 512 190 159 93 74 62 51 BspEI T'CCGG_A Cuts at: 4511 4511 Size: 5291 BspHI T'CATG_A Cuts at: 815 4467 815 Size: 3652 1639 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2341 2555 2857 3238 2341 Size: 214 302 381 4394 Fragments arranged by size: 4394 381 302 214 BsrBI CCG'CTC Cuts at: 50 1497 3583 3637 5121 5286 50 Size: 1447 2086 54 1484 165 55 Fragments arranged by size: 2086 1484 1447 165 55 54 BsrDI GCAATG_nn' Cuts at: 1589 3203 1589 Size: 1614 3677 BsrFI r'CCGG_y Cuts at: 308 3289 3470 3679 308 Size: 2981 181 209 1920 Fragments arranged by size: 2981 1920 209 181 BssHII G'CGCG_C Cuts at: 127 127 Size: 5291 BssSI C'ACGA_G Cuts at: 406 2157 3562 3920 406 Size: 1751 1405 358 1777 Fragments arranged by size: 1777 1751 1405 358 BstAPI GCAn_nnn'nTGC Cuts at: 1621 2668 1621 Size: 1047 4244 BstBI TT'CG_AA Cuts at: 560 3652 560 Size: 3092 2199 BstXI CCAn_nnnn'nTGG Cuts at: 1470 2163 1470 Size: 693 4598 BstYI r'GATC_y Cuts at: 1 733 942 2316 2783 2806 3141 3387 4399 Size: 732 209 1374 467 23 335 246 1012 Cuts at: 4399 4485 4497 4843 1 Size: 86 12 346 449 Fragments arranged by size: 1374 1012 732 467 449 346 335 246 209 86 23 12 Bsu36I CC'TnA_GG Cuts at: 454 1573 454 Size: 1119 4172 BtgI C'CryG_G Cuts at: 107 166 218 266 1115 107 Size: 59 52 48 849 4283 Fragments arranged by size: 4283 849 59 52 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3241 3422 3585 3241 Size: 181 163 4947 Fragments arranged by size: 4947 181 163 BtsI GCAGTG_nn' Cuts at: 1170 2534 1170 Size: 1364 3927 DraI TTT'AAA Cuts at: 2290 2290 Size: 5291 DraIII CAC_nnn'GTG Cuts at: 1957 1957 Size: 5291 DrdI GACnn_nn'nnGTC Cuts at: 2997 3855 2997 Size: 858 4433 EaeI y'GGCC_r Cuts at: 215 419 480 1598 2876 3050 3441 3468 4813 Size: 204 61 1118 1278 174 391 27 1345 Cuts at: 4813 215 Size: 693 Fragments arranged by size: 1345 1278 1118 693 391 204 174 61 27 EagI C'GGCC_G Cuts at: 215 2876 215 Size: 2661 2630 EarI CTCTTCn'nnn_ Cuts at: 382 703 1742 2179 3314 3524 382 Size: 321 1039 437 1135 210 2149 Fragments arranged by size: 2149 1135 1039 437 321 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 265 310 3499 3809 3955 5101 265 Size: 45 3189 310 146 1146 455 Fragments arranged by size: 3189 1146 455 310 146 45 EcoICRI GAG'CTC Cuts at: 276 2303 276 Size: 2027 3264 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 507 1207 1236 1764 1957 2185 2227 3116 3548 Size: 700 29 528 193 228 42 889 432 Cuts at: 3548 4295 4563 507 Size: 747 268 1235 Fragments arranged by size: 1235 889 747 700 528 432 268 228 193 42 29 EcoO109I rG'GnC_Cy Cuts at: 518 2430 518 Size: 1912 3379 EcoRI G'AATT_C Cuts at: 2295 2295 Size: 5291 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 732 1362 3310 732 Size: 630 1948 2713 Fragments arranged by size: 2713 1948 630 FspI TGC'GCA Cuts at: 3072 3072 Size: 5291 HaeII r_GCGC'y Cuts at: 160 266 1198 2725 2973 3995 4974 160 Size: 106 932 1527 248 1022 979 477 Fragments arranged by size: 1527 1022 979 932 477 248 106 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 866 898 956 988 1600 1632 1715 1747 3133 Size: 32 58 32 612 32 83 32 1386 Cuts at: 3133 3163 3165 3195 866 Size: 30 2 30 2962 Fragments arranged by size: 2962 1386 612 83 58 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1794 2330 1794 Size: 536 4755 KasI G'GCGC_C Cuts at: 156 2721 2969 156 Size: 2565 248 2478 Fragments arranged by size: 2565 2478 248 KpnI G_GTAC'C Cuts at: 467 2311 467 Size: 1844 3447 MluI A'CGCG_T Cuts at: 4724 4724 Size: 5291 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3962 4146 3962 Size: 184 5107 MscI TGG'CCA Cuts at: 421 1600 3052 421 Size: 1179 1452 2660 Fragments arranged by size: 2660 1452 1179 MslI CAynn'nnrTG Cuts at: 1558 3280 3407 1558 Size: 1722 127 3442 Fragments arranged by size: 3442 1722 127 MspA1I CmG'CkG Cuts at: 168 186 220 500 548 1030 2402 2716 3076 Size: 18 34 280 48 482 1372 314 360 Cuts at: 3076 4089 4334 168 Size: 1013 245 1125 Fragments arranged by size: 1372 1125 1013 482 360 314 280 245 48 34 18 NaeI GCC'GGC Cuts at: 3472 3472 Size: 5291 NarI GG'CG_CC Cuts at: 157 2722 2970 157 Size: 2565 248 2478 Fragments arranged by size: 2565 2478 248 NcoI C'CATG_G Cuts at: 107 266 1115 107 Size: 159 849 4283 Fragments arranged by size: 4283 849 159 NgoMIV G'CCGG_C Cuts at: 3470 3470 Size: 5291 NotI GC'GGCC_GC Cuts at: 215 215 Size: 5291 NspI r_CATG'y Cuts at: 934 2344 2844 3751 934 Size: 1410 500 907 2474 Fragments arranged by size: 2474 1410 907 500 PciI A'CATG_T Cuts at: 3747 3747 Size: 5291 PfoI T'CCnGG_A Cuts at: 1213 1213 Size: 5291 PmeI GTTT'AAAC Cuts at: 2290 2290 Size: 5291 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4430 4462 4430 Size: 32 5259 PstI C_TGCA'G Cuts at: 2338 3023 2338 Size: 685 4606 PvuI CG_AT'CG Cuts at: 104 104 Size: 5291 PvuII CAG'CTG Cuts at: 500 2716 3076 500 Size: 2216 360 2715 Fragments arranged by size: 2715 2216 360 RsrII CG'GwC_CG Cuts at: 3486 3486 Size: 5291 SacI G_AGCT'C Cuts at: 278 2305 278 Size: 2027 3264 SacII CC_GC'GG Cuts at: 169 221 169 Size: 52 5239 SalI G'TCGA_C Cuts at: 2328 2328 Size: 5291 SapI GCTCTTCn'nnn_ Cuts at: 382 2179 3314 3524 382 Size: 1797 1135 210 2149 Fragments arranged by size: 2149 1797 1135 210 SbfI CC_TGCA'GG Cuts at: 2338 2338 Size: 5291 ScaI AGT'ACT Cuts at: 1777 4808 1777 Size: 3031 2260 SfcI C'TryA_G Cuts at: 33 2334 3019 4012 4203 33 Size: 2301 685 993 191 1121 Fragments arranged by size: 2301 1121 993 685 191 SfoI GGC'GCC Cuts at: 158 2723 2971 158 Size: 2565 248 2478 Fragments arranged by size: 2565 2478 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5291 SmaI CCC'GGG Cuts at: 2313 2313 Size: 5291 SmlI C'TyrA_G Cuts at: 2136 2446 3361 3853 4115 2136 Size: 310 915 492 262 3312 Fragments arranged by size: 3312 915 492 310 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5291 SphI G_CATG'C Cuts at: 934 2344 2844 934 Size: 1410 500 3381 Fragments arranged by size: 3381 1410 500 StuI AGG'CCT Cuts at: 1571 1571 Size: 5291 StyI C'CwwG_G Cuts at: 107 266 1115 2425 2779 107 Size: 159 849 1310 354 2619 Fragments arranged by size: 2619 1310 849 354 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1713 1830 2095 2874 3007 3466 3675 1713 Size: 117 265 779 133 459 209 3329 Fragments arranged by size: 3329 779 459 265 209 133 117 TatI w'GTAC_w Cuts at: 996 1775 4806 996 Size: 779 3031 1481 Fragments arranged by size: 3031 1481 779 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2137 2169 4808 4840 2137 Size: 32 2639 32 2588 Fragments arranged by size: 2639 2588 32 32 Tth111I GACn'n_nGTC Cuts at: 3088 3088 Size: 5291 XbaI T'CTAG_A Cuts at: 2322 2322 Size: 5291 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2786 2786 Size: 5291 XmaI C'CCGG_G Cuts at: 2311 2311 Size: 5291 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI ApaI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI BsrGI BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI