(Linear) (Six-base) MAPSORT of: KIEE2692.seq Check: 9268 from: 1 to: 2178 KIEE2692.orf With 182 enzymes: SgfI * June 23, 2017 13:27 .. Acc65I G'GTAC_C Cuts at: 0 355 2178 Size: 355 1823 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 399 1656 1849 2178 Size: 399 1257 193 329 Fragments arranged by size: 1257 399 329 193 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1149 2178 Size: 1149 1029 AlwNI CAG_nnn'CTG Cuts at: 0 796 1753 2014 2178 Size: 796 957 261 164 Fragments arranged by size: 957 796 261 164 ApoI r'AATT_y Cuts at: 0 736 1434 2178 Size: 736 698 744 Fragments arranged by size: 744 736 698 AscI GG'CGCG_CC Cuts at: 0 19 2178 Size: 19 2159 AvaI C'yCGr_G Cuts at: 0 30 2178 Size: 30 2148 BanI G'GyrC_C Cuts at: 0 48 355 2143 2178 Size: 48 307 1788 35 Fragments arranged by size: 1788 307 48 35 BanII G_rGCy'C Cuts at: 0 170 329 1634 2178 Size: 170 159 1305 544 Fragments arranged by size: 1305 544 170 159 BbeI G_GCGC'C Cuts at: 0 52 2178 Size: 52 2126 BbsI GAAGACnn'nnnn_ Cuts at: 0 410 1958 2178 Size: 410 1548 220 Fragments arranged by size: 1548 410 220 BciVI GTATCCnnnnn_n' Cuts at: 0 580 2178 Size: 580 1598 BclI T'GATC_A Cuts at: 0 1815 2178 Size: 1815 363 BglI GCCn_nnn'nGGC Cuts at: 0 60 2178 Size: 60 2118 Bme1580I G_kGCm'C Cuts at: 0 2146 2178 Size: 2146 32 BmgBI CAC'GTC Cuts at: 0 831 2178 Size: 831 1347 BmrI ACTGGGnnnn_n' Cuts at: 0 784 2178 Size: 784 1394 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1650 1971 2178 Size: 1650 321 207 Fragments arranged by size: 1650 321 207 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1099 1128 2077 2119 2178 Size: 1099 29 949 42 59 Fragments arranged by size: 1099 949 59 42 29 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2013 2178 Size: 2013 165 BsaI GGTCTCn'nnnn_ Cuts at: 0 295 2099 2114 2178 Size: 295 1804 15 64 Fragments arranged by size: 1804 295 64 15 BsaHI Gr'CG_yC Cuts at: 0 49 1622 2178 Size: 49 1573 556 Fragments arranged by size: 1573 556 49 BsaWI w'CCGG_w Cuts at: 0 1766 2178 Size: 1766 412 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 848 878 1334 1364 1375 1405 2029 2059 Size: 848 30 456 30 11 30 624 30 Cuts at: 2059 2178 Size: 119 Fragments arranged by size: 848 624 456 119 30 30 30 30 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 114 117 178 205 1592 1642 2016 2178 Size: 114 3 61 27 1387 50 374 162 Fragments arranged by size: 1387 374 162 114 61 50 27 3 BseYI C'CCAG_C Cuts at: 0 84 2178 Size: 84 2094 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 378 1045 2178 Size: 280 98 667 1133 Fragments arranged by size: 1133 667 280 98 BsiEI CG_ry'CG Cuts at: 0 110 2178 Size: 110 2068 BsiHKAI G_wGCw'C Cuts at: 0 170 1114 2178 Size: 170 944 1064 Fragments arranged by size: 1064 944 170 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1075 2178 Size: 1075 1103 Bsp1286I G_dGCh'C Cuts at: 0 170 329 1114 1634 2146 2178 Size: 170 159 785 520 512 32 Fragments arranged by size: 785 520 512 170 159 32 BspHI T'CATG_A Cuts at: 0 707 2178 Size: 707 1471 BsrBI CCG'CTC Cuts at: 0 1389 2178 Size: 1389 789 BsrDI GCAATG_nn' Cuts at: 0 1481 2178 Size: 1481 697 BsrFI r'CCGG_y Cuts at: 0 200 2178 Size: 200 1978 BssHII G'CGCG_C Cuts at: 0 19 2178 Size: 19 2159 BssSI C'ACGA_G Cuts at: 0 298 2049 2178 Size: 298 1751 129 Fragments arranged by size: 1751 298 129 BstAPI GCAn_nnn'nTGC Cuts at: 0 1513 2178 Size: 1513 665 BstBI TT'CG_AA Cuts at: 0 452 2178 Size: 452 1726 BstXI CCAn_nnnn'nTGG Cuts at: 0 1362 2055 2178 Size: 1362 693 123 Fragments arranged by size: 1362 693 123 BstYI r'GATC_y Cuts at: 0 625 834 2178 Size: 625 209 1344 Fragments arranged by size: 1344 625 209 Bsu36I CC'TnA_GG Cuts at: 0 346 1465 2178 Size: 346 1119 713 Fragments arranged by size: 1119 713 346 BtgI C'CryG_G Cuts at: 0 58 110 158 1007 2178 Size: 58 52 48 849 1171 Fragments arranged by size: 1171 849 58 52 48 BtsI GCAGTG_nn' Cuts at: 0 1062 2178 Size: 1062 1116 DraIII CAC_nnn'GTG Cuts at: 0 1849 2178 Size: 1849 329 EaeI y'GGCC_r Cuts at: 0 107 311 372 1490 2178 Size: 107 204 61 1118 688 Fragments arranged by size: 1118 688 204 107 61 EagI C'GGCC_G Cuts at: 0 107 2178 Size: 107 2071 EarI CTCTTCn'nnn_ Cuts at: 0 274 595 1634 2071 2178 Size: 274 321 1039 437 107 Fragments arranged by size: 1039 437 321 274 107 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 157 202 2178 Size: 157 45 1976 Fragments arranged by size: 1976 157 45 EcoICRI GAG'CTC Cuts at: 0 168 2178 Size: 168 2010 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 399 1099 1128 1656 1849 2077 2119 2178 Size: 399 700 29 528 193 228 42 59 Fragments arranged by size: 700 528 399 228 193 59 42 29 EcoO109I rG'GnC_Cy Cuts at: 0 410 2178 Size: 410 1768 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 624 1254 2178 Size: 624 630 924 Fragments arranged by size: 924 630 624 HaeII r_GCGC'y Cuts at: 0 52 158 1090 2178 Size: 52 106 932 1088 Fragments arranged by size: 1088 932 106 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 758 790 848 880 1492 1524 1607 1639 Size: 758 32 58 32 612 32 83 32 Cuts at: 1639 2178 Size: 539 Fragments arranged by size: 758 612 539 83 58 32 32 32 32 HincII GTy'rAC Cuts at: 0 1686 2178 Size: 1686 492 KasI G'GCGC_C Cuts at: 0 48 2178 Size: 48 2130 KpnI G_GTAC'C Cuts at: 0 359 2178 Size: 359 1819 MscI TGG'CCA Cuts at: 0 313 1492 2178 Size: 313 1179 686 Fragments arranged by size: 1179 686 313 MslI CAynn'nnrTG Cuts at: 0 1450 2178 Size: 1450 728 MspA1I CmG'CkG Cuts at: 0 60 78 112 392 440 922 2178 Size: 60 18 34 280 48 482 1256 Fragments arranged by size: 1256 482 280 60 48 34 18 NarI GG'CG_CC Cuts at: 0 49 2178 Size: 49 2129 NcoI C'CATG_G Cuts at: 0 158 1007 2178 Size: 158 849 1171 Fragments arranged by size: 1171 849 158 NotI GC'GGCC_GC Cuts at: 0 107 2178 Size: 107 2071 NspI r_CATG'y Cuts at: 0 826 2178 Size: 826 1352 PfoI T'CCnGG_A Cuts at: 0 1105 2178 Size: 1105 1073 PvuII CAG'CTG Cuts at: 0 392 2178 Size: 392 1786 SacI G_AGCT'C Cuts at: 0 170 2178 Size: 170 2008 SacII CC_GC'GG Cuts at: 0 61 113 2178 Size: 61 52 2065 Fragments arranged by size: 2065 61 52 SapI GCTCTTCn'nnn_ Cuts at: 0 274 2071 2178 Size: 274 1797 107 Fragments arranged by size: 1797 274 107 ScaI AGT'ACT Cuts at: 0 1669 2178 Size: 1669 509 SfoI GGC'GCC Cuts at: 0 50 2178 Size: 50 2128 SmlI C'TyrA_G Cuts at: 0 2028 2178 Size: 2028 150 SphI G_CATG'C Cuts at: 0 826 2178 Size: 826 1352 StuI AGG'CCT Cuts at: 0 1463 2178 Size: 1463 715 StyI C'CwwG_G Cuts at: 0 158 1007 2178 Size: 158 849 1171 Fragments arranged by size: 1171 849 158 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1605 1722 1987 2178 Size: 1605 117 265 191 Fragments arranged by size: 1605 265 191 117 TatI w'GTAC_w Cuts at: 0 888 1667 2178 Size: 888 779 511 Fragments arranged by size: 888 779 511 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2029 2061 2178 Size: 2029 32 117 Fragments arranged by size: 2029 117 32 Enzymes that do cut: Acc65I AcuI AlfI AlwNI ApoI AscI AvaI BanI BanII BbeI BbsI BciVI BclI BglI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI KpnI MscI MslI MspA1I NarI NcoI NotI NspI PfoI PvuII SacI SacII SapI ScaI SfoI SmlI SphI StuI StyI TaqII TatI TstI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BfrBI BglII BlpI BmtI Bpu10I BsaAI BsaBI BsiWI BsmI BspEI BspMI BsrGI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI MmeI NaeI NdeI NgoMIV NheI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SalI SanDI SbfI SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI