(Circular) (Six-base) MAPSORT of: pF1KE2631.seq Check: 5222 from: 1 to: 6461 pF1KE2631 6461 bp With 182 enzymes: SgfI * July 8, 2016 11:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 832 832 Size: 6461 AccI GT'mk_AC Cuts at: 1442 3499 1442 Size: 2057 4404 Acc65I G'GTAC_C Cuts at: 3477 3477 Size: 6461 AclI AA'CG_TT Cuts at: 2537 6301 2537 Size: 3764 2697 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1269 2221 3189 4286 4718 5465 5733 1269 Size: 952 968 1097 432 747 268 1997 Fragments arranged by size: 1997 1097 968 952 747 432 268 AfeI AGC'GCT Cuts at: 2566 2566 Size: 6461 AflIII A'CryG_T Cuts at: 1814 3813 4917 5894 1814 Size: 1999 1104 977 2381 Fragments arranged by size: 2381 1999 1104 977 AgeI A'CCGG_T Cuts at: 4849 4849 Size: 6461 AleI CACnn'nnGTG Cuts at: 472 814 838 3265 472 Size: 342 24 2427 3668 Fragments arranged by size: 3668 2427 342 24 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 832 2638 832 Size: 1806 4655 AlwNI CAG_nnn'CTG Cuts at: 1848 2130 3766 5333 1848 Size: 282 1636 1567 2976 Fragments arranged by size: 2976 1636 1567 282 ApaI G_GGCC'C Cuts at: 628 1626 1719 2099 3169 628 Size: 998 93 380 1070 3920 Fragments arranged by size: 3920 1070 998 380 93 ApaLI G'TGCA_C Cuts at: 2167 2602 5231 2167 Size: 435 2629 3397 Fragments arranged by size: 3397 2629 435 ApoI r'AATT_y Cuts at: 3465 5814 6070 3465 Size: 2349 256 3856 Fragments arranged by size: 3856 2349 256 AseI AT'TA_AT Cuts at: 20 20 Size: 6461 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6461 AvaI C'yCGr_G Cuts at: 156 1647 2395 3299 3379 3481 156 Size: 1491 748 904 80 102 3136 Fragments arranged by size: 3136 1491 904 748 102 80 BamHI G'GATC_C Cuts at: 1576 3486 1576 Size: 1910 4551 BanI G'GyrC_C Cuts at: 400 640 700 808 1408 2245 2297 2444 2645 Size: 240 60 108 600 837 52 147 201 Cuts at: 2645 3477 3891 4139 4174 400 Size: 832 414 248 35 2687 Fragments arranged by size: 2687 837 832 600 414 248 240 201 147 108 60 52 35 BanII G_rGCy'C Cuts at: 628 1189 1626 1719 2099 2233 3169 3475 4505 Size: 561 437 93 380 134 936 306 1030 Cuts at: 4505 628 Size: 2584 Fragments arranged by size: 2584 1030 936 561 437 380 306 134 93 BbeI G_GCGC'C Cuts at: 2301 2448 3895 4143 2301 Size: 147 1447 248 4619 Fragments arranged by size: 4619 1447 248 147 BbsI GAAGACnn'nnnn_ Cuts at: 873 1275 2827 873 Size: 402 1552 4507 Fragments arranged by size: 4507 1552 402 BbvCI CC'TCA_GC Cuts at: 1852 1852 Size: 6461 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 1242 1276 3022 3056 90 Size: 34 1118 34 1746 34 3495 Fragments arranged by size: 3495 1746 1118 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2033 4359 5120 6461 2033 Size: 2326 761 1341 2033 Fragments arranged by size: 2326 2033 1341 761 BclI T'GATC_A Cuts at: 3981 3981 Size: 6461 BfrBI ATG'CAT Cuts at: 2787 2865 2787 Size: 78 6383 BglI GCCn_nnn'nGGC Cuts at: 1021 2278 1021 Size: 1257 5204 BglII A'GATC_T Cuts at: 3976 6013 3976 Size: 2037 4424 BlpI GC'TnA_GC Cuts at: 3573 3573 Size: 6461 Bme1580I G_kGCm'C Cuts at: 403 628 670 703 1540 1626 1667 1719 2099 Size: 225 42 33 837 86 41 52 380 Cuts at: 2099 2171 2248 2606 3169 4086 4179 5235 403 Size: 72 77 358 563 917 93 1056 1629 Fragments arranged by size: 1629 1056 917 837 563 380 358 225 93 86 77 72 52 42 41 33 BmgBI CAC'GTC Cuts at: 2468 2468 Size: 6461 BmrI ACTGGGnnnn_n' Cuts at: 3164 3673 3754 4089 6233 3164 Size: 509 81 335 2144 3392 Fragments arranged by size: 3392 2144 509 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2586 2635 4828 2586 Size: 49 2193 4219 Fragments arranged by size: 4219 2193 49 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1203 1887 2107 2136 1203 Size: 684 220 29 5528 Fragments arranged by size: 5528 684 220 29 Bpu10I CC'TnA_GC Cuts at: 1852 2561 1852 Size: 709 5752 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2260 3637 4516 5008 5306 2260 Size: 1377 879 492 298 3415 Fragments arranged by size: 3415 1377 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 623 6165 623 Size: 5542 919 BsaAI yAC'GTr Cuts at: 3814 3814 Size: 6461 BsaBI GATnn'nnATC Cuts at: 6 1750 2677 3975 6012 6 Size: 1744 927 1298 2037 455 Fragments arranged by size: 2037 1744 1298 927 455 BsaHI Gr'CG_yC Cuts at: 446 2298 2445 3305 3892 4140 4842 6338 446 Size: 1852 147 860 587 248 702 1496 569 Fragments arranged by size: 1852 1496 860 702 587 569 248 147 BsaWI w'CCGG_w Cuts at: 2930 3108 3650 4171 4849 5123 5270 5681 2930 Size: 178 542 521 678 274 147 411 3710 Fragments arranged by size: 3710 678 542 521 411 274 178 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1435 1465 1435 Size: 30 6431 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 268 271 1065 1233 2337 3025 3028 3094 3171 Size: 3 794 168 1104 688 3 66 77 Cuts at: 3171 268 Size: 3558 Fragments arranged by size: 3558 1104 794 688 168 77 66 3 3 BseYI C'CCAG_C Cuts at: 732 922 1600 2174 2195 2273 3046 3055 3886 Size: 190 678 574 21 78 773 9 831 Cuts at: 3886 4644 5221 5717 732 Size: 758 577 496 1476 Fragments arranged by size: 1476 831 773 758 678 577 574 496 190 78 21 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 403 1856 1861 3040 403 Size: 1453 5 1179 3824 Fragments arranged by size: 3824 1453 1179 5 BsiEI CG_ry'CG Cuts at: 104 3266 3432 4003 4049 5257 104 Size: 3162 166 571 46 1208 1308 Fragments arranged by size: 3162 1308 1208 571 166 46 BsiHKAI G_wGCw'C Cuts at: 821 1189 2171 2606 3045 3398 3475 4253 4443 Size: 368 982 435 439 353 77 778 190 Cuts at: 4443 5235 821 Size: 792 2047 Fragments arranged by size: 2047 982 792 778 439 435 368 353 190 77 BsmBI CGTCTCn'nnnn_ Cuts at: 1033 2974 1033 Size: 1941 4520 Bsp1286I G_dGCh'C Cuts at: 403 628 670 703 821 1189 1540 1626 1667 Size: 225 42 33 118 368 351 86 41 Cuts at: 1667 1719 2099 2171 2233 2248 2606 3045 3169 Size: 52 380 72 62 15 358 439 124 Cuts at: 3169 3398 3475 4086 4179 4253 4443 4505 5235 Size: 229 77 611 93 74 190 62 730 Cuts at: 5235 403 Size: 1629 Fragments arranged by size: 1629 730 611 439 380 368 358 351 229 225 190 124 118 93 86 77 74 72 62 62 52 42 41 33 15 BspEI T'CCGG_A Cuts at: 2930 3108 5681 2930 Size: 178 2573 3710 Fragments arranged by size: 3710 2573 178 BspHI T'CATG_A Cuts at: 5637 5637 Size: 6461 BspMI ACCTGCnnnn'nnnn_ Cuts at: 326 428 832 1665 3511 3725 4027 4408 326 Size: 102 404 833 1846 214 302 381 2379 Fragments arranged by size: 2379 1846 833 404 381 302 214 102 BsrBI CCG'CTC Cuts at: 50 1367 2883 2889 4753 4807 6291 6456 50 Size: 1317 1516 6 1864 54 1484 165 55 Fragments arranged by size: 1864 1516 1484 1317 165 55 54 6 BsrDI GCAATG_nn' Cuts at: 482 1757 1976 3357 4373 482 Size: 1275 219 1381 1016 2570 Fragments arranged by size: 2570 1381 1275 1016 219 BsrFI r'CCGG_y Cuts at: 378 4459 4640 4849 378 Size: 4081 181 209 1990 Fragments arranged by size: 4081 1990 209 181 BsrGI T'GTAC_A Cuts at: 689 689 Size: 6461 BssSI C'ACGA_G Cuts at: 1528 2918 4732 5090 1528 Size: 1390 1814 358 2899 Fragments arranged by size: 2899 1814 1390 358 BstAPI GCAn_nnn'nTGC Cuts at: 760 1848 3838 760 Size: 1088 1990 3383 Fragments arranged by size: 3383 1990 1088 BstBI TT'CG_AA Cuts at: 4822 4822 Size: 6461 BstEII G'GTnAC_C Cuts at: 976 1044 2526 976 Size: 68 1482 4911 Fragments arranged by size: 4911 1482 68 BstXI CCAn_nnnn'nTGG Cuts at: 276 621 1559 276 Size: 345 938 5178 Fragments arranged by size: 5178 938 345 BstYI r'GATC_y Cuts at: 1 1133 1576 2727 2849 2894 3105 3486 3953 Size: 1132 443 1151 122 45 211 381 467 Cuts at: 3953 3976 4311 4557 5569 5655 5667 6013 1 Size: 23 335 246 1012 86 12 346 449 Fragments arranged by size: 1151 1132 1012 467 449 443 381 346 335 246 211 122 86 45 23 12 Bsu36I CC'TnA_GG Cuts at: 1378 3086 1378 Size: 1708 4753 BtgI C'CryG_G Cuts at: 644 737 996 1558 1686 2010 3420 644 Size: 93 259 562 128 324 1410 3685 Fragments arranged by size: 3685 1410 562 324 259 128 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 155 1407 2048 4411 4592 4755 155 Size: 1252 641 2363 181 163 1861 Fragments arranged by size: 2363 1861 1252 641 181 163 BtsI GCAGTG_nn' Cuts at: 1844 2759 3704 1844 Size: 915 945 4601 Fragments arranged by size: 4601 945 915 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 345 380 345 Size: 35 6426 DraI TTT'AAA Cuts at: 3460 3460 Size: 6461 DrdI GACnn_nn'nnGTC Cuts at: 4167 5025 4167 Size: 858 5603 EaeI y'GGCC_r Cuts at: 302 545 740 830 911 1244 1418 1549 1683 Size: 243 195 90 81 333 174 131 134 Cuts at: 1683 2508 3263 3429 4046 4220 4611 4638 5983 Size: 825 755 166 617 174 391 27 1345 Cuts at: 5983 302 Size: 780 Fragments arranged by size: 1345 825 780 755 617 391 333 243 195 174 174 166 134 131 90 81 27 EagI C'GGCC_G Cuts at: 3263 3429 4046 3263 Size: 166 617 5678 Fragments arranged by size: 5678 617 166 EarI CTCTTCn'nnn_ Cuts at: 338 460 733 2279 2972 2993 4484 4694 338 Size: 122 273 1546 693 21 1491 210 2105 Fragments arranged by size: 2105 1546 1491 693 273 210 122 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2676 2950 4669 4979 5125 6271 2676 Size: 274 1719 310 146 1146 2866 Fragments arranged by size: 2866 1719 1146 310 274 146 EcoICRI GAG'CTC Cuts at: 1187 3473 1187 Size: 2286 4175 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1203 1269 1887 2107 2136 2221 3189 4286 4718 Size: 66 618 220 29 85 968 1097 432 Cuts at: 4718 5465 5733 1203 Size: 747 268 1931 Fragments arranged by size: 1931 1097 968 747 618 432 268 220 85 66 29 EcoNI CCTnn'n_nnAGG Cuts at: 322 1198 1636 1771 2305 322 Size: 876 438 135 534 4478 Fragments arranged by size: 4478 876 534 438 135 EcoO109I rG'GnC_Cy Cuts at: 387 1062 1374 1469 1622 1623 1716 2095 2096 Size: 675 312 95 153 1 93 379 1 Cuts at: 2096 2214 2391 3165 3166 3384 3600 387 Size: 118 177 774 1 218 216 3248 Fragments arranged by size: 3248 774 675 379 312 218 216 177 153 118 95 93 1 1 1 EcoRI G'AATT_C Cuts at: 3465 3465 Size: 6461 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 957 3219 4480 957 Size: 2262 1261 2938 Fragments arranged by size: 2938 2262 1261 FspI TGC'GCA Cuts at: 4242 4242 Size: 6461 HaeII r_GCGC'y Cuts at: 2301 2448 2568 3895 4143 5165 6144 2301 Size: 147 120 1327 248 1022 979 2618 Fragments arranged by size: 2618 1327 1022 979 248 147 120 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1282 1314 2031 2063 2353 2385 2912 2925 2944 Size: 32 717 32 290 32 527 13 19 Cuts at: 2944 2957 3018 3050 4303 4333 4335 4365 1282 Size: 13 61 32 1253 30 2 30 3378 Fragments arranged by size: 3378 1253 717 527 290 61 32 32 32 32 30 30 19 13 13 2 HincII GTy'rAC Cuts at: 864 3500 864 Size: 2636 3825 HindIII A'AGCT_T Cuts at: 3310 3310 Size: 6461 KasI G'GCGC_C Cuts at: 2297 2444 3891 4139 2297 Size: 147 1447 248 4619 Fragments arranged by size: 4619 1447 248 147 KpnI G_GTAC'C Cuts at: 3481 3481 Size: 6461 MfeI C'AATT_G Cuts at: 563 563 Size: 6461 MluI A'CGCG_T Cuts at: 5894 5894 Size: 6461 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2375 3266 5132 5316 2375 Size: 891 1866 184 3520 Fragments arranged by size: 3520 1866 891 184 MscI TGG'CCA Cuts at: 304 742 832 913 1420 1551 1685 2510 4222 Size: 438 90 81 507 131 134 825 1712 Cuts at: 4222 304 Size: 2543 Fragments arranged by size: 2543 1712 825 507 438 134 131 90 81 MslI CAynn'nnrTG Cuts at: 406 412 472 553 814 826 838 1056 1277 Size: 6 60 81 261 12 12 218 221 Cuts at: 1277 1557 1819 2620 2716 3265 4450 4577 406 Size: 280 262 801 96 549 1185 127 2290 Fragments arranged by size: 2290 1185 801 549 280 262 261 221 218 127 96 81 60 12 12 6 MspA1I CmG'CkG Cuts at: 2127 2178 2199 2277 2506 3024 3050 3422 3572 Size: 51 21 78 229 518 26 372 150 Cuts at: 3572 3886 4246 5259 5504 2127 Size: 314 360 1013 245 3084 Fragments arranged by size: 3084 1013 518 372 360 314 245 229 150 78 51 26 21 NaeI GCC'GGC Cuts at: 4642 4642 Size: 6461 NarI GG'CG_CC Cuts at: 2298 2445 3892 4140 2298 Size: 147 1447 248 4619 Fragments arranged by size: 4619 1447 248 147 NcoI C'CATG_G Cuts at: 737 996 1558 1686 737 Size: 259 562 128 5512 Fragments arranged by size: 5512 562 259 128 NgoMIV G'CCGG_C Cuts at: 4640 4640 Size: 6461 NotI GC'GGCC_GC Cuts at: 3429 3429 Size: 6461 NsiI A_TGCA'T Cuts at: 2789 2867 2789 Size: 78 6383 NspI r_CATG'y Cuts at: 798 1818 2619 3258 3330 3514 4014 4921 798 Size: 1020 801 639 72 184 500 907 2338 Fragments arranged by size: 2338 1020 907 801 639 500 184 72 PasI CC'CwG_GG Cuts at: 324 3073 324 Size: 2749 3712 PciI A'CATG_T Cuts at: 1814 4917 1814 Size: 3103 3358 PflMI CCAn_nnn'nTGG Cuts at: 634 2114 2163 634 Size: 1480 49 4932 Fragments arranged by size: 4932 1480 49 PfoI T'CCnGG_A Cuts at: 2492 2492 Size: 6461 PmeI GTTT'AAAC Cuts at: 3460 3460 Size: 6461 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5600 5632 5600 Size: 32 6429 PpuMI rG'GwC_Cy Cuts at: 1374 3384 1374 Size: 2010 4451 PspOMI G'GGCC_C Cuts at: 624 1622 1715 2095 3165 624 Size: 998 93 380 1070 3920 Fragments arranged by size: 3920 1070 998 380 93 PstI C_TGCA'G Cuts at: 434 1610 1640 1676 2408 3508 4193 434 Size: 1176 30 36 732 1100 685 2702 Fragments arranged by size: 2702 1176 1100 732 685 36 30 PvuI CG_AT'CG Cuts at: 104 104 Size: 6461 PvuII CAG'CTG Cuts at: 2127 2178 2199 2506 3024 3050 3886 4246 2127 Size: 51 21 307 518 26 836 360 4342 Fragments arranged by size: 4342 836 518 360 307 51 26 21 RsrII CG'GwC_CG Cuts at: 4656 4656 Size: 6461 SacI G_AGCT'C Cuts at: 1189 3475 1189 Size: 2286 4175 SacII CC_GC'GG Cuts at: 3423 3423 Size: 6461 SalI G'TCGA_C Cuts at: 3498 3498 Size: 6461 SapI GCTCTTCn'nnn_ Cuts at: 4484 4694 4484 Size: 210 6251 SbfI CC_TGCA'GG Cuts at: 1676 3508 1676 Size: 1832 4629 ScaI AGT'ACT Cuts at: 5978 5978 Size: 6461 SfcI C'TryA_G Cuts at: 33 430 1606 1636 1672 2404 3504 4189 5182 Size: 397 1176 30 36 732 1100 685 993 Cuts at: 5182 5373 33 Size: 191 1121 Fragments arranged by size: 1176 1121 1100 993 732 685 397 191 36 30 SfoI GGC'GCC Cuts at: 2299 2446 3893 4141 2299 Size: 147 1447 248 4619 Fragments arranged by size: 4619 1447 248 147 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6461 SmaI CCC'GGG Cuts at: 158 3301 3483 158 Size: 3143 182 3136 Fragments arranged by size: 3143 3136 182 SmlI C'TyrA_G Cuts at: 1647 2239 3379 3616 4531 5023 5285 1647 Size: 592 1140 237 915 492 262 2823 Fragments arranged by size: 2823 1140 915 592 492 262 237 SpeI A'CTAG_T Cuts at: 67 67 Size: 6461 SphI G_CATG'C Cuts at: 2619 3258 3514 4014 2619 Size: 639 256 500 5066 Fragments arranged by size: 5066 639 500 256 StyI C'CwwG_G Cuts at: 173 737 996 1199 1558 1686 3134 3595 3949 Size: 564 259 203 359 128 1448 461 354 Cuts at: 3949 173 Size: 2685 Fragments arranged by size: 2685 1448 564 461 359 354 259 203 128 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 462 838 966 1875 4044 4177 4636 4845 462 Size: 376 128 909 2169 133 459 209 2078 Fragments arranged by size: 2169 2078 909 459 376 209 133 128 TatI w'GTAC_w Cuts at: 689 1997 5976 689 Size: 1308 3979 1174 Fragments arranged by size: 3979 1308 1174 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 941 973 5978 6010 941 Size: 32 5005 32 1392 Fragments arranged by size: 5005 1392 32 32 Tth111I GACn'n_nGTC Cuts at: 4258 4258 Size: 6461 XbaI T'CTAG_A Cuts at: 3492 3492 Size: 6461 XcmI CCAnnnn_n'nnnnTGG Cuts at: 693 1144 3956 693 Size: 451 2812 3198 Fragments arranged by size: 3198 2812 451 XhoI C'TCGA_G Cuts at: 1647 3379 1647 Size: 1732 4729 XmaI C'CCGG_G Cuts at: 156 3299 3481 156 Size: 3143 182 3136 Fragments arranged by size: 3143 3136 182 XmnI GAAnn'nnTTC Cuts at: 2326 2326 Size: 6461 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AflII AhdI AloI AscI AvrII BaeI BmtI BsiWI BsmI BssHII BstZ17I ClaI DraIII EcoRV FseI FspAI HpaI NdeI NheI NruI PacI PmlI PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI ZraI