################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BciVI BfrI BglII BlnI BsaAI BseAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DsaI EarI Eco81I EcoO65I EcoRI EcoT14I EcoT22I FauI FseI FspI HpaI KasI KpnI Ksp632I KspI MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 774 CGCG CG^CG AcsI 261 AAATTC [GA]^AATT[CT] AcyI 1257 GACGCC G[GA]^CG[CT]C AhdI 309 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 483 GATCC GGATC(4/5) Aor51HI 129 AGCGCT AGC^GCT ApoI 261 AAATTC [GA]^AATT[CT] AspEI 309 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 394 GGCACC G^G[CT][GA]CC BcgI 578 GCAAGGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 180 TGATCA T^GATCA BfaI 793 CTAG C^TAG BglI 343 GCCTTGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 175 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1257 GACGCC G[GA]^CG[CT]C BsaI 556 GAGACC GGTCTC(1/5) BsaWI 416 ACCGGA [AT]^CCGG[AT] BsgI 437 GTGCAG GTGCAG(16/14) BsiEI 350 CGGCCG CG[GA][CT]^CG BsrBI 634 GAGCGG CCGCTC(-3/-3) BsrDI 944 GCAATG GCAATG(2/0) BstUI 774 CGCG CG^CG BstXI 456 CCAGAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 1276 GACGGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 350 CGGCCG C^GGCCG Eam1105I 309 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 350 CGGCCG C^GGCCG Eco47III 129 AGCGCT AGC^GCT Eco52I 350 CGGCCG C^GGCCG EcoNI 624 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1308 GATATC GAT^ATC FbaI 180 TGATCA T^GATCA HgaI 1257 GACGC GACGC(5/10) Hin1I 1257 GACGCC G[GA]^CG[CT]C HincII 1285 GTCAAC GT[CT]^[GA]AC HindII 1285 GTCAAC GT[CT]^[GA]AC MaeI 793 CTAG C^TAG MamI 175 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 1163 TTAA T^TAA MvnI 774 CGCG CG^CG NdeI 1002 CATATG CA^TATG PstI 644 CTGCAG CTGCA^G PvuII 781 CAGCTG CAG^CTG SfcI 644 CTGCAG C^T[GA][CT]AG SspI 1385 AATATT AAT^ATT Tru9I 1163 TTAA T^TAA XcmI 189 CCAGAGTGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### BcnI 380 CCGGG CC^[GC]GG 615 CCCGG BlpI 132 GCTCAGC GC^T[ACGT]AGC 739 GCTGAGC Bpu1102I 132 GCTCAGC GC^T[ACGT]AGC 739 GCTGAGC BsmFI 1255 GGGAC GGGAC(10/14) 1207 GTCCC BspHI 305 TCATGA T^CATGA 884 TCATGA Cac8I 47 GCAAGC GC[ACGT]^[ACGT]GC 137 GCAAGC CelII 132 GCTCAGC GC^T[ACGT]AGC 739 GCTGAGC CfoI 130 GCGC GCG^C 971 GCGC DraII 677 AGGACCC [GA]G^G[ACGT]CC[CT] 871 AGGACCC DraIII 526 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG 1199 CACCCTGTG EcoO109I 677 AGGACCC [GA]G^G[ACGT]CC[CT] 871 AGGACCC HaeII 129 AGCGCT [GA]GCGC^[CT] 970 AGCGCC HhaI 130 GCGC GCG^C 971 GCGC HinP1I 130 GCGC G^CGC 971 GCGC HindIII 1115 AAGCTT A^AGCTT 1138 AAGCTT MaeII 515 ACGT A^CGT 1087 ACGT MslI 714 CACGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 901 CACTTCAATG MspA1I 781 CAGCTG C[AC]G^C[GT]G 1008 CCGCTG NciI 380 CCGGG CC^[GC]GG 615 CCCGG PpuMI 677 AGGACCC [GA]G^G[AT]CC[CT] 871 AGGACCC RcaI 305 TCATGA T^CATGA 884 TCATGA TaqI 977 TCGA T^CGA 1247 TCGA TthHB8I 977 TCGA T^CGA 1247 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BanII BbsI BbvI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI SacI Sau3AI Sau96I ScrFI TfiI TseI Tsp45I Tsp509I TspRI