(Circular) (Six-base) MAPSORT of: pF1KE2637.seq Check: 7971 from: 1 to: 5387 pF1KE2637 5387 bp With 182 enzymes: SgfI * February 19, 2021 20:23 .. AatII G_ACGT'C Cuts at: 779 779 Size: 5387 AccI GT'mk_AC Cuts at: 1985 2318 2425 1985 Size: 333 107 4947 Fragments arranged by size: 4947 333 107 Acc65I G'GTAC_C Cuts at: 2403 2403 Size: 5387 AclI AA'CG_TT Cuts at: 5227 5227 Size: 5387 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 437 736 1837 1876 3212 3644 4391 4659 437 Size: 299 1101 39 1336 432 747 268 1165 Fragments arranged by size: 1336 1165 1101 747 432 299 268 39 AflIII A'CryG_T Cuts at: 1297 1953 2264 2739 3843 4820 1297 Size: 656 311 475 1104 977 1864 Fragments arranged by size: 1864 1104 977 656 475 311 AgeI A'CCGG_T Cuts at: 3775 3775 Size: 5387 AhdI GACnn_n'nnGTC Cuts at: 2352 2352 Size: 5387 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1202 1202 Size: 5387 AlwNI CAG_nnn'CTG Cuts at: 631 2692 4259 631 Size: 2061 1567 1759 Fragments arranged by size: 2061 1759 1567 ApaI G_GGCC'C Cuts at: 595 968 1098 1126 595 Size: 373 130 28 4856 Fragments arranged by size: 4856 373 130 28 ApaLI G'TGCA_C Cuts at: 4157 4157 Size: 5387 ApoI r'AATT_y Cuts at: 2391 4740 4996 2391 Size: 2349 256 2782 Fragments arranged by size: 2782 2349 256 AseI AT'TA_AT Cuts at: 20 1892 20 Size: 1872 3515 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5387 AvaI C'yCGr_G Cuts at: 1118 1718 1959 2407 1118 Size: 600 241 448 4098 Fragments arranged by size: 4098 600 448 241 BamHI G'GATC_C Cuts at: 2412 2412 Size: 5387 BanI G'GyrC_C Cuts at: 895 2403 2817 3065 3100 895 Size: 1508 414 248 35 3182 Fragments arranged by size: 3182 1508 414 248 35 BanII G_rGCy'C Cuts at: 119 289 360 576 595 959 968 1098 1126 Size: 170 71 216 19 364 9 130 28 Cuts at: 1126 1169 1727 2401 3431 119 Size: 43 558 674 1030 2075 Fragments arranged by size: 2075 1030 674 558 364 216 170 130 71 43 28 19 9 BbeI G_GCGC'C Cuts at: 2821 3069 2821 Size: 248 5139 BbsI GAAGACnn'nnnn_ Cuts at: 458 773 1760 458 Size: 315 987 4085 Fragments arranged by size: 4085 987 315 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2338 2372 2338 Size: 34 5353 BciVI GTATCCnnnnn_n' Cuts at: 3285 4046 5387 3285 Size: 761 1341 3285 Fragments arranged by size: 3285 1341 761 BclI T'GATC_A Cuts at: 2907 2907 Size: 5387 BglI GCCn_nnn'nGGC Cuts at: 963 1547 2365 963 Size: 584 818 3985 Fragments arranged by size: 3985 818 584 BglII A'GATC_T Cuts at: 2902 4939 2902 Size: 2037 3350 BlpI GC'TnA_GC Cuts at: 113 2499 113 Size: 2386 3001 Bme1580I G_kGCm'C Cuts at: 595 968 1098 1126 1405 1805 3012 3105 4161 Size: 373 130 28 279 400 1207 93 1056 Cuts at: 4161 595 Size: 1821 Fragments arranged by size: 1821 1207 1056 400 373 279 130 93 28 BmgBI CAC'GTC Cuts at: 1956 2145 2267 1956 Size: 189 122 5076 Fragments arranged by size: 5076 189 122 BmrI ACTGGGnnnn_n' Cuts at: 962 2599 2680 3015 5159 962 Size: 1637 81 335 2144 1190 Fragments arranged by size: 2144 1637 1190 335 81 BmtI G_CTAG'C Cuts at: 2344 2344 Size: 5387 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3754 3754 Size: 5387 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1024 1121 1371 1484 1520 1024 Size: 97 250 113 36 4891 Fragments arranged by size: 4891 250 113 97 36 Bpu10I CC'TnA_GC Cuts at: 662 1336 2002 662 Size: 674 666 4047 Fragments arranged by size: 4047 674 666 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 655 1468 2563 3442 3934 4232 655 Size: 813 1095 879 492 298 1810 Fragments arranged by size: 1810 1095 879 813 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 683 5091 683 Size: 4408 979 BsaAI yAC'GTr Cuts at: 471 1020 2740 471 Size: 549 1720 3118 Fragments arranged by size: 3118 1720 549 BsaBI GATnn'nnATC Cuts at: 6 2901 4938 6 Size: 2895 2037 455 Fragments arranged by size: 2895 2037 455 BsaHI Gr'CG_yC Cuts at: 776 2818 3066 3768 5264 776 Size: 2042 248 702 1496 899 Fragments arranged by size: 2042 1496 899 702 248 BsaWI w'CCGG_w Cuts at: 2576 3097 3775 4049 4196 4607 2576 Size: 521 678 274 147 411 3356 Fragments arranged by size: 3356 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 997 1027 2221 2251 997 Size: 30 1194 30 4133 Fragments arranged by size: 4133 1194 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 769 1078 2283 769 Size: 309 1205 3873 Fragments arranged by size: 3873 1205 309 BseYI C'CCAG_C Cuts at: 151 442 822 885 945 1606 2327 2812 3570 Size: 291 380 63 60 661 721 485 758 Cuts at: 3570 4147 4643 151 Size: 577 496 895 Fragments arranged by size: 895 758 721 661 577 496 485 380 291 63 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 679 1060 1903 679 Size: 381 843 4163 Fragments arranged by size: 4163 843 381 BsiEI CG_ry'CG Cuts at: 104 651 2929 2975 4183 104 Size: 547 2278 46 1208 1308 Fragments arranged by size: 2278 1308 1208 547 46 BsiHKAI G_wGCw'C Cuts at: 313 360 2401 3179 3369 4161 313 Size: 47 2041 778 190 792 1539 Fragments arranged by size: 2041 1539 792 778 190 47 BsiWI C'GTAC_G Cuts at: 158 158 Size: 5387 BsmBI CGTCTCn'nnnn_ Cuts at: 1437 2273 1437 Size: 836 4551 Bsp1286I G_dGCh'C Cuts at: 119 289 313 360 576 595 959 968 1098 Size: 170 24 47 216 19 364 9 130 Cuts at: 1098 1126 1169 1405 1727 1805 2401 3012 3105 Size: 28 43 236 322 78 596 611 93 Cuts at: 3105 3179 3369 3431 4161 119 Size: 74 190 62 730 1345 Fragments arranged by size: 1345 730 611 596 364 322 236 216 190 170 130 93 78 74 62 47 43 28 24 19 9 BspEI T'CCGG_A Cuts at: 4607 4607 Size: 5387 BspHI T'CATG_A Cuts at: 4563 4563 Size: 5387 BspMI ACCTGCnnnn'nnnn_ Cuts at: 701 2437 2651 2953 3334 701 Size: 1736 214 302 381 2754 Fragments arranged by size: 2754 1736 381 302 214 BsrBI CCG'CTC Cuts at: 50 1591 1685 3679 3733 5217 5382 50 Size: 1541 94 1994 54 1484 165 55 Fragments arranged by size: 1994 1541 1484 165 94 55 54 BsrDI GCAATG_nn' Cuts at: 2292 3299 2292 Size: 1007 4380 BsrFI r'CCGG_y Cuts at: 2299 3385 3566 3775 2299 Size: 1086 181 209 3911 Fragments arranged by size: 3911 1086 209 181 BsrGI T'GTAC_A Cuts at: 2237 2237 Size: 5387 BssSI C'ACGA_G Cuts at: 3658 4016 3658 Size: 358 5029 BstAPI GCAn_nnn'nTGC Cuts at: 2764 2764 Size: 5387 BstBI TT'CG_AA Cuts at: 131 3748 131 Size: 3617 1770 BstEII G'GTnAC_C Cuts at: 2010 2010 Size: 5387 BstXI CCAn_nnnn'nTGG Cuts at: 1734 2149 1734 Size: 415 4972 BstYI r'GATC_y Cuts at: 1 271 395 941 1905 2412 2879 2902 3237 Size: 270 124 546 964 507 467 23 335 Cuts at: 3237 3483 4495 4581 4593 4939 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1012 964 546 507 467 449 346 335 270 246 124 86 23 12 Bsu36I CC'TnA_GG Cuts at: 587 632 587 Size: 45 5342 BtgI C'CryG_G Cuts at: 107 1195 1519 2220 2272 107 Size: 1088 324 701 52 3222 Fragments arranged by size: 3222 1088 701 324 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1286 3337 3518 3681 1286 Size: 2051 181 163 2992 Fragments arranged by size: 2992 2051 181 163 BtsI GCAGTG_nn' Cuts at: 1745 1977 2630 1745 Size: 232 653 4502 Fragments arranged by size: 4502 653 232 DraI TTT'AAA Cuts at: 2386 2386 Size: 5387 DrdI GACnn_nn'nnGTC Cuts at: 775 3093 3951 775 Size: 2318 858 2211 Fragments arranged by size: 2318 2211 858 EaeI y'GGCC_r Cuts at: 648 2061 2366 2972 3146 3537 3564 4909 648 Size: 1413 305 606 174 391 27 1345 1126 Fragments arranged by size: 1413 1345 1126 606 391 305 174 27 EagI C'GGCC_G Cuts at: 648 2972 648 Size: 2324 3063 EarI CTCTTCn'nnn_ Cuts at: 1440 3410 3620 1440 Size: 1970 210 3207 Fragments arranged by size: 3207 1970 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1761 2168 2242 3595 3905 4051 5197 1761 Size: 407 74 1353 310 146 1146 1951 Fragments arranged by size: 1951 1353 1146 407 310 146 74 EcoICRI GAG'CTC Cuts at: 358 2399 358 Size: 2041 3346 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 437 736 1024 1121 1371 1484 1520 1837 1876 Size: 299 288 97 250 113 36 317 39 Cuts at: 1876 3212 3644 4391 4659 437 Size: 1336 432 747 268 1165 Fragments arranged by size: 1336 1165 747 432 317 299 288 268 250 113 97 39 36 EcoNI CCTnn'n_nnAGG Cuts at: 586 697 2005 2153 586 Size: 111 1308 148 3820 Fragments arranged by size: 3820 1308 148 111 EcoO109I rG'GnC_Cy Cuts at: 529 591 592 628 1094 1095 1122 1123 1218 Size: 62 1 36 466 1 27 1 95 Cuts at: 1218 2123 2526 529 Size: 905 403 3390 Fragments arranged by size: 3390 905 466 403 95 62 36 27 1 1 1 EcoRI G'AATT_C Cuts at: 2391 2391 Size: 5387 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2109 2205 3406 2109 Size: 96 1201 4090 Fragments arranged by size: 4090 1201 96 FspI TGC'GCA Cuts at: 3168 3168 Size: 5387 HaeII r_GCGC'y Cuts at: 2821 3069 4091 5070 2821 Size: 248 1022 979 3138 Fragments arranged by size: 3138 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 129 161 256 288 772 804 907 939 2338 Size: 32 95 32 484 32 103 32 1399 Cuts at: 2338 2370 3229 3259 3261 3291 129 Size: 32 859 30 2 30 2225 Fragments arranged by size: 2225 1399 859 484 103 95 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1998 2426 1998 Size: 428 4959 HindIII A'AGCT_T Cuts at: 2086 2086 Size: 5387 KasI G'GCGC_C Cuts at: 2817 3065 2817 Size: 248 5139 KpnI G_GTAC'C Cuts at: 2407 2407 Size: 5387 MluI A'CGCG_T Cuts at: 4820 4820 Size: 5387 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 340 1404 1729 4058 4242 340 Size: 1064 325 2329 184 1485 Fragments arranged by size: 2329 1485 1064 325 184 MscI TGG'CCA Cuts at: 2063 2368 3148 2063 Size: 305 780 4302 Fragments arranged by size: 4302 780 305 MslI CAynn'nnrTG Cuts at: 2311 3376 3503 2311 Size: 1065 127 4195 Fragments arranged by size: 4195 1065 127 MspA1I CmG'CkG Cuts at: 322 415 714 889 1197 1521 1643 2498 2812 Size: 93 299 175 308 324 122 855 314 Cuts at: 2812 3172 4185 4430 322 Size: 360 1013 245 1279 Fragments arranged by size: 1279 1013 855 360 324 314 308 299 245 175 122 93 NaeI GCC'GGC Cuts at: 3568 3568 Size: 5387 NarI GG'CG_CC Cuts at: 2818 3066 2818 Size: 248 5139 NcoI C'CATG_G Cuts at: 107 107 Size: 5387 NgoMIV G'CCGG_C Cuts at: 3566 3566 Size: 5387 NheI G'CTAG_C Cuts at: 2340 2340 Size: 5387 NotI GC'GGCC_GC Cuts at: 648 648 Size: 5387 NspI r_CATG'y Cuts at: 567 2440 2940 3847 567 Size: 1873 500 907 2107 Fragments arranged by size: 2107 1873 907 500 PciI A'CATG_T Cuts at: 3843 3843 Size: 5387 PflMI CCAn_nnn'nTGG Cuts at: 2059 2059 Size: 5387 PfoI T'CCnGG_A Cuts at: 503 503 Size: 5387 PmeI GTTT'AAAC Cuts at: 2386 2386 Size: 5387 PmlI CAC'GTG Cuts at: 471 1020 471 Size: 549 4838 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4526 4558 4526 Size: 32 5355 PpuMI rG'GwC_Cy Cuts at: 628 1218 2123 628 Size: 590 905 3892 Fragments arranged by size: 3892 905 590 PspOMI G'GGCC_C Cuts at: 591 964 1094 1122 591 Size: 373 130 28 4856 Fragments arranged by size: 4856 373 130 28 PstI C_TGCA'G Cuts at: 254 1790 2434 3119 254 Size: 1536 644 685 2522 Fragments arranged by size: 2522 1536 685 644 PvuI CG_AT'CG Cuts at: 104 104 Size: 5387 PvuII CAG'CTG Cuts at: 415 2812 3172 415 Size: 2397 360 2630 Fragments arranged by size: 2630 2397 360 RsrII CG'GwC_CG Cuts at: 3582 3582 Size: 5387 SacI G_AGCT'C Cuts at: 360 2401 360 Size: 2041 3346 SacII CC_GC'GG Cuts at: 1198 1522 1198 Size: 324 5063 SalI G'TCGA_C Cuts at: 2424 2424 Size: 5387 SanDI GG'GwC_CC Cuts at: 1218 2123 1218 Size: 905 4482 SapI GCTCTTCn'nnn_ Cuts at: 3410 3620 3410 Size: 210 5177 SbfI CC_TGCA'GG Cuts at: 2434 2434 Size: 5387 ScaI AGT'ACT Cuts at: 4904 4904 Size: 5387 SfcI C'TryA_G Cuts at: 33 250 1647 1786 2430 3115 4108 4299 33 Size: 217 1397 139 644 685 993 191 1121 Fragments arranged by size: 1397 1121 993 685 644 217 191 139 SfiI GGCCn_nnn'nGGCC Cuts at: 1547 1547 Size: 5387 SfoI GGC'GCC Cuts at: 2819 3067 2819 Size: 248 5139 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5387 SmaI CCC'GGG Cuts at: 1120 1720 1961 2409 1120 Size: 600 241 448 4098 Fragments arranged by size: 4098 600 448 241 SmlI C'TyrA_G Cuts at: 670 1483 2542 3457 3949 4211 670 Size: 813 1059 915 492 262 1846 Fragments arranged by size: 1846 1059 915 813 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5387 SphI G_CATG'C Cuts at: 567 2440 2940 567 Size: 1873 500 3014 Fragments arranged by size: 3014 1873 500 StuI AGG'CCT Cuts at: 817 1329 817 Size: 512 4875 StyI C'CwwG_G Cuts at: 107 812 2521 2875 107 Size: 705 1709 354 2619 Fragments arranged by size: 2619 1709 705 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1047 1620 1728 1770 2155 2970 3103 3562 3771 Size: 573 108 42 385 815 133 459 209 Cuts at: 3771 1047 Size: 2663 Fragments arranged by size: 2663 815 573 459 385 209 133 108 42 TatI w'GTAC_w Cuts at: 2237 4902 2237 Size: 2665 2722 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1554 1586 4904 4936 1554 Size: 32 3318 32 2005 Fragments arranged by size: 3318 2005 32 32 Tth111I GACn'n_nGTC Cuts at: 2104 3184 2104 Size: 1080 4307 XbaI T'CTAG_A Cuts at: 2418 2418 Size: 5387 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1927 2116 2376 2882 1927 Size: 189 260 506 4432 Fragments arranged by size: 4432 506 260 189 XmaI C'CCGG_G Cuts at: 1118 1718 1959 2407 1118 Size: 600 241 448 4098 Fragments arranged by size: 4098 600 448 241 ZraI GAC'GTC Cuts at: 777 777 Size: 5387 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI AfeI AflII AleI AloI AscI AvrII BaeI BbvCI BfrBI BsmI BssHII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI NruI NsiI PacI PasI PshAI PsiI PspXI PsrI SexAI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI