(Linear) (Six-base) MAPSORT of: KIEE2637.seq Check: 4669 from: 1 to: 2274 KIEE2637.orf With 182 enzymes: SgfI * February 19, 2021 20:23 .. AatII G_ACGT'C Cuts at: 0 671 2274 Size: 671 1603 AccI GT'mk_AC Cuts at: 0 1877 2210 2274 Size: 1877 333 64 Fragments arranged by size: 1877 333 64 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 329 628 1729 1768 2274 Size: 329 299 1101 39 506 Fragments arranged by size: 1101 506 329 299 39 AflIII A'CryG_T Cuts at: 0 1189 1845 2156 2274 Size: 1189 656 311 118 Fragments arranged by size: 1189 656 311 118 AhdI GACnn_n'nnGTC Cuts at: 0 2244 2274 Size: 2244 30 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1094 2274 Size: 1094 1180 AlwNI CAG_nnn'CTG Cuts at: 0 523 2274 Size: 523 1751 ApaI G_GGCC'C Cuts at: 0 487 860 990 1018 2274 Size: 487 373 130 28 1256 Fragments arranged by size: 1256 487 373 130 28 AseI AT'TA_AT Cuts at: 0 1784 2274 Size: 1784 490 AvaI C'yCGr_G Cuts at: 0 1010 1610 1851 2274 Size: 1010 600 241 423 Fragments arranged by size: 1010 600 423 241 BanI G'GyrC_C Cuts at: 0 787 2274 Size: 787 1487 BanII G_rGCy'C Cuts at: 0 11 181 252 468 487 851 860 990 Size: 11 170 71 216 19 364 9 130 Cuts at: 990 1018 1061 1619 2274 Size: 28 43 558 655 Fragments arranged by size: 655 558 364 216 170 130 71 43 28 19 11 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 350 665 1652 2274 Size: 350 315 987 622 Fragments arranged by size: 987 622 350 315 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2230 2264 2274 Size: 2230 34 10 Fragments arranged by size: 2230 34 10 BglI GCCn_nnn'nGGC Cuts at: 0 855 1439 2257 2274 Size: 855 584 818 17 Fragments arranged by size: 855 818 584 17 BlpI GC'TnA_GC Cuts at: 0 5 2274 Size: 5 2269 Bme1580I G_kGCm'C Cuts at: 0 487 860 990 1018 1297 1697 2274 Size: 487 373 130 28 279 400 577 Fragments arranged by size: 577 487 400 373 279 130 28 BmgBI CAC'GTC Cuts at: 0 1848 2037 2159 2274 Size: 1848 189 122 115 Fragments arranged by size: 1848 189 122 115 BmrI ACTGGGnnnn_n' Cuts at: 0 854 2274 Size: 854 1420 BmtI G_CTAG'C Cuts at: 0 2236 2274 Size: 2236 38 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 916 1013 1263 1376 1412 2274 Size: 916 97 250 113 36 862 Fragments arranged by size: 916 862 250 113 97 36 Bpu10I CC'TnA_GC Cuts at: 0 554 1228 1894 2274 Size: 554 674 666 380 Fragments arranged by size: 674 666 554 380 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 547 1360 2274 Size: 547 813 914 Fragments arranged by size: 914 813 547 BsaI GGTCTCn'nnnn_ Cuts at: 0 575 2274 Size: 575 1699 BsaAI yAC'GTr Cuts at: 0 363 912 2274 Size: 363 549 1362 Fragments arranged by size: 1362 549 363 BsaHI Gr'CG_yC Cuts at: 0 668 2274 Size: 668 1606 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 889 919 2113 2143 2274 Size: 889 30 1194 30 131 Fragments arranged by size: 1194 889 131 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 661 970 2175 2274 Size: 661 309 1205 99 Fragments arranged by size: 1205 661 309 99 BseYI C'CCAG_C Cuts at: 0 43 334 714 777 837 1498 2219 2274 Size: 43 291 380 63 60 661 721 55 Fragments arranged by size: 721 661 380 291 63 60 55 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 571 952 1795 2274 Size: 571 381 843 479 Fragments arranged by size: 843 571 479 381 BsiEI CG_ry'CG Cuts at: 0 543 2274 Size: 543 1731 BsiHKAI G_wGCw'C Cuts at: 0 205 252 2274 Size: 205 47 2022 Fragments arranged by size: 2022 205 47 BsiWI C'GTAC_G Cuts at: 0 50 2274 Size: 50 2224 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1329 2165 2274 Size: 1329 836 109 Fragments arranged by size: 1329 836 109 Bsp1286I G_dGCh'C Cuts at: 0 11 181 205 252 468 487 851 860 Size: 11 170 24 47 216 19 364 9 Cuts at: 860 990 1018 1061 1297 1619 1697 2274 Size: 130 28 43 236 322 78 577 Fragments arranged by size: 577 364 322 236 216 170 130 78 47 43 28 24 19 11 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 593 2274 Size: 593 1681 BsrBI CCG'CTC Cuts at: 0 1483 1577 2274 Size: 1483 94 697 Fragments arranged by size: 1483 697 94 BsrDI GCAATG_nn' Cuts at: 0 2184 2274 Size: 2184 90 BsrFI r'CCGG_y Cuts at: 0 2191 2274 Size: 2191 83 BsrGI T'GTAC_A Cuts at: 0 2129 2274 Size: 2129 145 BstBI TT'CG_AA Cuts at: 0 23 2274 Size: 23 2251 BstEII G'GTnAC_C Cuts at: 0 1902 2274 Size: 1902 372 BstXI CCAn_nnnn'nTGG Cuts at: 0 1626 2041 2274 Size: 1626 415 233 Fragments arranged by size: 1626 415 233 BstYI r'GATC_y Cuts at: 0 163 287 833 1797 2274 Size: 163 124 546 964 477 Fragments arranged by size: 964 546 477 163 124 Bsu36I CC'TnA_GG Cuts at: 0 479 524 2274 Size: 479 45 1750 Fragments arranged by size: 1750 479 45 BtgI C'CryG_G Cuts at: 0 1087 1411 2112 2164 2274 Size: 1087 324 701 52 110 Fragments arranged by size: 1087 701 324 110 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1178 2274 Size: 1178 1096 BtsI GCAGTG_nn' Cuts at: 0 1637 1869 2274 Size: 1637 232 405 Fragments arranged by size: 1637 405 232 DrdI GACnn_nn'nnGTC Cuts at: 0 667 2274 Size: 667 1607 EaeI y'GGCC_r Cuts at: 0 540 1953 2258 2274 Size: 540 1413 305 16 Fragments arranged by size: 1413 540 305 16 EagI C'GGCC_G Cuts at: 0 540 2274 Size: 540 1734 EarI CTCTTCn'nnn_ Cuts at: 0 1332 2274 Size: 1332 942 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1653 2060 2134 2274 Size: 1653 407 74 140 Fragments arranged by size: 1653 407 140 74 EcoICRI GAG'CTC Cuts at: 0 250 2274 Size: 250 2024 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 329 628 916 1013 1263 1376 1412 1729 Size: 329 299 288 97 250 113 36 317 Cuts at: 1729 1768 2274 Size: 39 506 Fragments arranged by size: 506 329 317 299 288 250 113 97 39 36 EcoNI CCTnn'n_nnAGG Cuts at: 0 478 589 1897 2045 2274 Size: 478 111 1308 148 229 Fragments arranged by size: 1308 478 229 148 111 EcoO109I rG'GnC_Cy Cuts at: 0 421 483 484 520 986 987 1014 1015 Size: 421 62 1 36 466 1 27 1 Cuts at: 1015 1110 2015 2274 Size: 95 905 259 Fragments arranged by size: 905 466 421 259 95 62 36 27 1 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2001 2097 2274 Size: 2001 96 177 Fragments arranged by size: 2001 177 96 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 21 53 148 180 664 696 799 831 Size: 21 32 95 32 484 32 103 32 Cuts at: 831 2230 2262 2274 Size: 1399 32 12 Fragments arranged by size: 1399 484 103 95 32 32 32 32 32 21 12 HincII GTy'rAC Cuts at: 0 1890 2274 Size: 1890 384 HindIII A'AGCT_T Cuts at: 0 1978 2274 Size: 1978 296 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 232 1296 1621 2274 Size: 232 1064 325 653 Fragments arranged by size: 1064 653 325 232 MscI TGG'CCA Cuts at: 0 1955 2260 2274 Size: 1955 305 14 Fragments arranged by size: 1955 305 14 MslI CAynn'nnrTG Cuts at: 0 2203 2274 Size: 2203 71 MspA1I CmG'CkG Cuts at: 0 214 307 606 781 1089 1413 1535 2274 Size: 214 93 299 175 308 324 122 739 Fragments arranged by size: 739 324 308 299 214 175 122 93 NheI G'CTAG_C Cuts at: 0 2232 2274 Size: 2232 42 NotI GC'GGCC_GC Cuts at: 0 540 2274 Size: 540 1734 NspI r_CATG'y Cuts at: 0 459 2274 Size: 459 1815 PflMI CCAn_nnn'nTGG Cuts at: 0 1951 2274 Size: 1951 323 PfoI T'CCnGG_A Cuts at: 0 395 2274 Size: 395 1879 PmlI CAC'GTG Cuts at: 0 363 912 2274 Size: 363 549 1362 Fragments arranged by size: 1362 549 363 PpuMI rG'GwC_Cy Cuts at: 0 520 1110 2015 2274 Size: 520 590 905 259 Fragments arranged by size: 905 590 520 259 PspOMI G'GGCC_C Cuts at: 0 483 856 986 1014 2274 Size: 483 373 130 28 1260 Fragments arranged by size: 1260 483 373 130 28 PstI C_TGCA'G Cuts at: 0 146 1682 2274 Size: 146 1536 592 Fragments arranged by size: 1536 592 146 PvuII CAG'CTG Cuts at: 0 307 2274 Size: 307 1967 SacI G_AGCT'C Cuts at: 0 252 2274 Size: 252 2022 SacII CC_GC'GG Cuts at: 0 1090 1414 2274 Size: 1090 324 860 Fragments arranged by size: 1090 860 324 SanDI GG'GwC_CC Cuts at: 0 1110 2015 2274 Size: 1110 905 259 Fragments arranged by size: 1110 905 259 SfcI C'TryA_G Cuts at: 0 142 1539 1678 2274 Size: 142 1397 139 596 Fragments arranged by size: 1397 596 142 139 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1439 2274 Size: 1439 835 SmaI CCC'GGG Cuts at: 0 1012 1612 1853 2274 Size: 1012 600 241 421 Fragments arranged by size: 1012 600 421 241 SmlI C'TyrA_G Cuts at: 0 562 1375 2274 Size: 562 813 899 Fragments arranged by size: 899 813 562 SphI G_CATG'C Cuts at: 0 459 2274 Size: 459 1815 StuI AGG'CCT Cuts at: 0 709 1221 2274 Size: 709 512 1053 Fragments arranged by size: 1053 709 512 StyI C'CwwG_G Cuts at: 0 704 2274 Size: 704 1570 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 939 1512 1620 1662 2047 2274 Size: 939 573 108 42 385 227 Fragments arranged by size: 939 573 385 227 108 42 TatI w'GTAC_w Cuts at: 0 2129 2274 Size: 2129 145 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1446 1478 2274 Size: 1446 32 796 Fragments arranged by size: 1446 796 32 Tth111I GACn'n_nGTC Cuts at: 0 1996 2274 Size: 1996 278 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1819 2008 2274 Size: 1819 189 266 Fragments arranged by size: 1819 266 189 XmaI C'CCGG_G Cuts at: 0 1010 1610 1851 2274 Size: 1010 600 241 423 Fragments arranged by size: 1010 600 423 241 ZraI GAC'GTC Cuts at: 0 669 2274 Size: 669 1605 Enzymes that do cut: AatII AccI AcuI AflIII AhdI AlfI AlwNI ApaI AseI AvaI BanI BanII BbsI BcgI BglI BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HindIII MmeI MscI MslI MspA1I NheI NotI NspI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SacII SanDI SfcI SfiI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AleI AloI ApaLI ApoI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglII BplI BsaBI BsaWI BsmI BspEI BspHI BssHII BssSI BstAPI BstZ17I ClaI CspCI DraI DraIII EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NruI NsiI PacI PasI PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmnI