################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AviII AvrII BamHI BanI BbeI BbvCI BciVI BclI BfrI BglII BlnI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsmI BspDI BspEI BspHI BspLU11I BspMI BssHII BssSI Bst1107I BstAPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HpaI KasI KpnI Ksp632I MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AhdI 1288 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 832 ATTAAT AT^TAAT AsnI 832 ATTAAT AT^TAAT AspEI 1288 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1042 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 361 CACGTG CAC^GTG BcgI 1290 CGAGGTGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1282 CTAG C^TAG BlpI 4 GCTGAGC GC^T[ACGT]AGC BpmI 476 CTCCAG CTGGAG(16/14) Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC BsaAI 361 CACGTG [CT]AC^GT[GA] BseRI 1233 CTCCTC GAGGAG(10/8) BsgI 823 GTGCAG GTGCAG(16/14) BsiWI 50 CGTACG C^GTACG BsmBI 1208 CGTCTC CGTCTC(1/5) Bsp1407I 1178 TGTACA T^GTACA BsrDI 1226 GCAATG GCAATG(2/0) BsrFI 1240 ACCGGC [GA]^CCGG[CT] BsrGI 1178 TGTACA T^GTACA BstBI 22 TTCGAA TT^CGAA BstEII 951 GGTGACC G^GT[ACGT]ACC BstPI 951 GGTGACC G^GT[ACGT]ACC CelII 4 GCTGAGC GC^T[ACGT]AGC CfoI 271 GCGC GCG^C Cfr10I 1240 ACCGGC [GA]^CCGG[CT] DraII 1063 GGGTCCC [GA]G^G[ACGT]CC[CT] Eam1105I 1288 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 1063 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 951 GGTGACC G^GT[ACGT]ACC EcoT14I 425 CCAAGG C^C[AT][AT]GG HgaI 118 GACGC GACGC(5/10) HhaI 271 GCGC GCG^C HinP1I 271 GCGC G^CGC HincII 937 GTTGAC GT[CT]^[GA]AC HindII 937 GTTGAC GT[CT]^[GA]AC HindIII 1027 AAGCTT A^AGCTT KspI 460 CCGCGG CCGC^GG MaeI 1282 CTAG C^TAG MseI 833 TTAA T^TAA MslI 1248 CATCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NheI 1281 GCTAGC G^CTAGC NspV 22 TTCGAA TT^CGAA PflFI 1042 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 994 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 361 CACGTG CAC^GTG PmlI 361 CACGTG CAC^GTG PpuMI 1063 GGGTCCC [GA]G^G[AT]CC[CT] PshBI 832 ATTAAT AT^TAAT PvuII 305 CAGCTG CAG^CTG SacI 248 GAGCTC GAGCT^C SacII 460 CCGCGG CCGC^GG SfiI 481 GGCCAGAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 22 TTCGAA TT^CGAA SspBI 1178 TGTACA T^GTACA StyI 425 CCAAGG C^C[AT][AT]GG Tru9I 833 TTAA T^TAA Tsp509I 831 AATT ^AATT Tth111I 1042 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 994 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 925 GTCTAC GT^[AC][GT]AC 1258 GTAGAC AflIII 894 ACACGT A^C[GA][CT]GT 1205 ACACGT AspHI 201 GAGCAC G[AT]GC[AT]^C 248 GAGCTC AvaI 659 CCCGGG C^[CT]CG[GA]G 900 CCCGGG AvaII 684 GGTCC G^G[AT]CC 1064 GGTCC BalI 1002 TGGCCA TGG^CCA 1307 TGGCCA BbsI 343 GAAGAC GAAGAC(2/6) 708 GTCTTC BpuAI 343 GAAGAC GAAGAC(2/6) 708 GTCTTC BsiHKAI 201 GAGCAC G[AT]GC[AT]^C 248 GAGCTC BsoBI 659 CCCGGG C^[CT]CG[GA]G 900 CCCGGG BsrBI 530 CCGCTC CCGCTC(-3/-3) 624 CCGCTC BstF5I 981 CATCC GGATG(2/0) 240 GGATG BstXI 668 CCAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1083 CCACGTCCCTGG EaeI 1002 TGGCCA [CT]^GGCC[GA] 1307 TGGCCA EcoNI 942 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1090 CCTGGCGGAGG FokI 981 CATCC GGATG(9/13) 240 GGATG MluNI 1002 TGGCCA TGG^CCA 1307 TGGCCA MscI 1002 TGGCCA TGG^CCA 1307 TGGCCA PleI 1261 GACTC GAGTC(4/5) 1046 GAGTC PstI 142 CTGCAG CTGCA^G 727 CTGCAG SmaI 659 CCCGGG CCC^GGG 900 CCCGGG TfiI 133 GAATC G^A[AT]TC 510 GATTC XcmI 861 CCAGTCCAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1050 CCAGTCGAAGATTGG XmaCI 659 CCCGGG C^CCGGG 900 CCCGGG XmaI 659 CCCGGG C^CCGGG 900 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AfaI AluI AlwI BanII BbvI BcnI BglI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI BstUI BstYI Cac8I Cfr13I DdeI DpnII DsaI EcoRII FauI Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I XhoII