Restriction map for pF1KE2677
 (Linear) (Six-base) MAPSORT of: KIEE2677.seq  Check: 7423  from: 1  to: 5460



KIEE2677.orf

 With 182 enzymes: SgfI * 

                        March 1, 2017 16:47  ..

AccI GT'mk_AC

Cuts at:      0     562    5460 
   Size:        562    4898
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59    1646    2018    2500    2594    3607    3817    5460 
   Size:         59    1587     372     482      94    1013     210    1643

  Fragments arranged by size: 

                1643    1587    1013     482     372     210      94      59
            
 
AfeI AGC'GCT

Cuts at:      0    3443    5460 
   Size:       3443    2017
 
AflIII A'CryG_T

Cuts at:      0    2841    5460 
   Size:       2841    2619
 
AleI CACnn'nnGTG

Cuts at:      0    1010    4306    4933    5460 
   Size:       1010    3296     627     527

  Fragments arranged by size: 

                3296    1010     627     527
 
AlwNI CAG_nnn'CTG

Cuts at:      0    3369    3819    4729    5460 
   Size:       3369     450     910     731

  Fragments arranged by size: 

                3369     910     731     450
 
ApoI r'AATT_y

Cuts at:      0      84     118     334     379     802     830     877    1346 
   Size:         84      34     216      45     423      28      47     469

Cuts at:   1346    1455    1792    1799    2344    2775    2804    3128    3150 
   Size:        109     337       7     545     431      29     324      22

Cuts at:   3150    3597    3607    4769    5041    5064    5194    5351    5460 
   Size:        447      10    1162     272      23     130     157     109

  Fragments arranged by size: 

                1162     545     469     447     431     423     337     324
                 272     216     157     130     109     109      84      47
                  45      34      29      28      23      22      10       7
            
 
AseI AT'TA_AT

Cuts at:      0    2191    2950    3959    5460 
   Size:       2191     759    1009    1501

  Fragments arranged by size: 

                2191    1501    1009     759
 
AvaI C'yCGr_G

Cuts at:      0    1952    5460 
   Size:       1952    3508
 
BanII G_rGCy'C

Cuts at:      0    1842    5269    5460 
   Size:       1842    3427     191

  Fragments arranged by size: 

                3427    1842     191
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     178     538    1171    1598    1748    2582    2630    4130 
   Size:        178     360     633     427     150     834      48    1500

Cuts at:   4130    5460 
   Size:       1330

  Fragments arranged by size: 

                1500    1330     834     633     427     360     178     150
                  48
 
BbvCI CC'TCA_GC

Cuts at:      0    4463    5460 
   Size:       4463     997
 
BclI T'GATC_A

Cuts at:      0    2319    4836    5415    5460 
   Size:       2319    2517     579      45

  Fragments arranged by size: 

                2517    2319     579      45
 
BfrBI ATG'CAT

Cuts at:      0     287    5460 
   Size:        287    5173
 
BglII A'GATC_T

Cuts at:      0     939    3828    4095    5460 
   Size:        939    2889     267    1365

  Fragments arranged by size: 

                2889    1365     939     267
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1208    5460 
   Size:       1208    4252
 
BmtI G_CTAG'C

Cuts at:      0     644    5460 
   Size:        644    4816
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3807    5460 
   Size:       3807    1653
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160    3988    5460 
   Size:        160    3828    1472

  Fragments arranged by size: 

                3828    1472     160
 
Bpu10I CC'TnA_GC

Cuts at:      0     315    4463    5460 
   Size:        315    4148     997

  Fragments arranged by size: 

                4148     997     315
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244    1448    1481    2275    3652    4015    4204    4397 
   Size:        244    1204      33     794    1377     363     189     193

Cuts at:   4397    5460 
   Size:       1063

  Fragments arranged by size: 

                1377    1204    1063     794     363     244     193     189
                  33
 
BsaAI yAC'GTr

Cuts at:      0    2977    5460 
   Size:       2977    2483
 
BsaBI GATnn'nnATC

Cuts at:      0    4405    5460 
   Size:       4405    1055
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1624    1726    3064    4999    5460 
   Size:       1624     102    1338    1935     461

  Fragments arranged by size: 

                1935    1624    1338     461     102
 
BseYI C'CCAG_C

Cuts at:      0     360    4517    5460 
   Size:        360    4157     943

  Fragments arranged by size: 

                4157     943     360
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2866    5460 
   Size:       2866    2594
 
BsiEI CG_ry'CG

Cuts at:      0    2605    5460 
   Size:       2605    2855
 
BsiHKAI G_wGCw'C

Cuts at:      0    5269    5460 
   Size:       5269     191
 
BsmI GAATG_Cn'

Cuts at:      0     509    5022    5460 
   Size:        509    4513     438

  Fragments arranged by size: 

                4513     509     438
 
Bsp1286I G_dGCh'C

Cuts at:      0    1842    5269    5460 
   Size:       1842    3427     191

  Fragments arranged by size: 

                3427    1842     191
 
BspHI T'CATG_A

Cuts at:      0     114    2882    5460 
   Size:        114    2768    2578

  Fragments arranged by size: 

                2768    2578     114
 
BsrDI GCAATG_nn'

Cuts at:      0     652    1421    2394    2609    4254    5460 
   Size:        652     769     973     215    1645    1206

  Fragments arranged by size: 

                1645    1206     973     769     652     215
 
BsrGI T'GTAC_A

Cuts at:      0    4620    5460 
   Size:       4620     840
 
BssSI C'ACGA_G

Cuts at:      0    1103    1708    5460 
   Size:       1103     605    3752

  Fragments arranged by size: 

                3752    1103     605
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3802    5460 
   Size:       3802    1658
 
BstBI TT'CG_AA

Cuts at:      0     158     189    5442    5460 
   Size:        158      31    5253      18

  Fragments arranged by size: 

                5253     158      31      18
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    4224    5460 
   Size:       4224    1236
 
BstYI r'GATC_y

Cuts at:      0     589     939    3030    3828    4095    4725    4795    5460 
   Size:        589     350    2091     798     267     630      70     665

  Fragments arranged by size: 

                2091     798     665     630     589     350     267      70
            
 
BstZ17I GTA'TAC

Cuts at:      0     563    5460 
   Size:        563    4897
 
Bsu36I CC'TnA_GG

Cuts at:      0    2459    5460 
   Size:       2459    3001
 
BtsI GCAGTG_nn'

Cuts at:      0    4913    5460 
   Size:       4913     547
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1971    2006    5460 
   Size:       1971      35    3454

  Fragments arranged by size: 

                3454    1971      35
 
DraI TTT'AAA

Cuts at:      0     989    1454    1797    2382    3653    5460 
   Size:        989     465     343     585    1271    1807

  Fragments arranged by size: 

                1807    1271     989     585     465     343
 
DraIII CAC_nnn'GTG

Cuts at:      0    3463    5460 
   Size:       3463    1997
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     434    5460 
   Size:        434    5026
 
EaeI y'GGCC_r

Cuts at:      0    4214    5162    5460 
   Size:       4214     948     298

  Fragments arranged by size: 

                4214     948     298
 
EarI CTCTTCn'nnn_

Cuts at:      0    2048    2111    2201    3216    4957    4963    5274    5460 
   Size:       2048      63      90    1015    1741       6     311     186

  Fragments arranged by size: 

                2048    1741    1015     311     186      90      63       6
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1407    5460 
   Size:       1407    4053
 
EcoICRI GAG'CTC

Cuts at:      0    5267    5460 
   Size:       5267     193
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     160    1646    2018    2500    2594    3607    3817 
   Size:         59     101    1486     372     482      94    1013     210

Cuts at:   3817    3988    5460 
   Size:        171    1472

  Fragments arranged by size: 

                1486    1472    1013     482     372     210     171     101
                  94      59
 
EcoRI G'AATT_C

Cuts at:      0     379    3128    5460 
   Size:        379    2749    2332

  Fragments arranged by size: 

                2749    2332     379
 
EcoRV GAT'ATC

Cuts at:      0    4610    4698    5460 
   Size:       4610      88     762

  Fragments arranged by size: 

                4610     762      88
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     399    3373    3637    3847    5460 
   Size:        399    2974     264     210    1613

  Fragments arranged by size: 

                2974    1613     399     264     210
 
HaeII r_GCGC'y

Cuts at:      0    3445    5460 
   Size:       3445    2015
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2177    2209    2455    2487    4780    4787    4812    4819 
   Size:       2177      32     246      32    2293       7      25       7

Cuts at:   4819    5460 
   Size:        641

  Fragments arranged by size: 

                2293    2177     641     246      32      32      25       7
                   7
 
HincII GTy'rAC

Cuts at:      0    3695    4826    5460 
   Size:       3695    1131     634

  Fragments arranged by size: 

                3695    1131     634
 
HindIII A'AGCT_T

Cuts at:      0     100    5460 
   Size:        100    5360
 
HpaI GTT'AAC

Cuts at:      0    3695    5460 
   Size:       3695    1765
 
MfeI C'AATT_G

Cuts at:      0      62    2828    5460 
   Size:         62    2766    2632

  Fragments arranged by size: 

                2766    2632      62
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1709    2823    5460 
   Size:       1709    1114    2637

  Fragments arranged by size: 

                2637    1709    1114
 
MscI TGG'CCA

Cuts at:      0    4216    5164    5460 
   Size:       4216     948     296

  Fragments arranged by size: 

                4216     948     296
 
MslI CAynn'nnrTG

Cuts at:      0    1010    2012    2382    4132    4306    4933    5460 
   Size:       1010    1002     370    1750     174     627     527

  Fragments arranged by size: 

                1750    1010    1002     627     527     370     174
 
MspA1I CmG'CkG

Cuts at:      0    3369    4521    5460 
   Size:       3369    1152     939

  Fragments arranged by size: 

                3369    1152     939
 
NdeI CA'TA_TG

Cuts at:      0    2366    5460 
   Size:       2366    3094
 
NheI G'CTAG_C

Cuts at:      0     640    5460 
   Size:        640    4820
 
NsiI A_TGCA'T

Cuts at:      0     289    5460 
   Size:        289    5171
 
NspI r_CATG'y

Cuts at:      0    1285    2845    3424    4091    4131    5460 
   Size:       1285    1560     579     667      40    1329

  Fragments arranged by size: 

                1560    1329    1285     667     579      40
 
PciI A'CATG_T

Cuts at:      0    2841    5460 
   Size:       2841    2619
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1562    5460 
   Size:       1562    3898
 
PfoI T'CCnGG_A

Cuts at:      0    1292    5460 
   Size:       1292    4168
 
PsiI TTA'TAA

Cuts at:      0     934    5460 
   Size:        934    4526
 
PvuII CAG'CTG

Cuts at:      0    3369    4521    5460 
   Size:       3369    1152     939

  Fragments arranged by size: 

                3369    1152     939
 
SacI G_AGCT'C

Cuts at:      0    5269    5460 
   Size:       5269     191
 
SapI GCTCTTCn'nnn_

Cuts at:      0    5274    5460 
   Size:       5274     186
 
SfcI C'TryA_G

Cuts at:      0     978    4799    5188    5460 
   Size:        978    3821     389     272

  Fragments arranged by size: 

                3821     978     389     272
 
SmlI C'TyrA_G

Cuts at:      0     223    1463    1496    1952    2254    3667    4030    4183 
   Size:        223    1240      33     456     302    1413     363     153

Cuts at:   4183    4412    5460 
   Size:        229    1048

  Fragments arranged by size: 

                1413    1240    1048     456     363     302     229     223
                 153      33
 
SnaBI TAC'GTA

Cuts at:      0    2977    5460 
   Size:       2977    2483
 
SphI G_CATG'C

Cuts at:      0    4091    5460 
   Size:       4091    1369
 
SspI AAT'ATT

Cuts at:      0     504    1076    1535    5460 
   Size:        504     572     459    3925

  Fragments arranged by size: 

                3925     572     504     459
 
StuI AGG'CCT

Cuts at:      0      72    3208    5460 
   Size:         72    3136    2252

  Fragments arranged by size: 

                3136    2252      72
 
StyI C'CwwG_G

Cuts at:      0     291     347    1787    4952    5460 
   Size:        291      56    1440    3165     508

  Fragments arranged by size: 

                3165    1440     508     291      56
 
SwaI ATTT'AAAT

Cuts at:      0    2382    3653    5460 
   Size:       2382    1271    1807

  Fragments arranged by size: 

                2382    1807    1271
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2619    5040    5460 
   Size:       2619    2421     420

  Fragments arranged by size: 

                2619    2421     420
 
TatI w'GTAC_w

Cuts at:      0    3903    4620    5460 
   Size:       3903     717     840

  Fragments arranged by size: 

                3903     840     717
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4836    4868    5460 
   Size:       4836      32     592

  Fragments arranged by size: 

                4836     592      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     431    5460 
   Size:        431    5029
 
XbaI T'CTAG_A

Cuts at:      0    1575    5460 
   Size:       1575    3885
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     783    5460 
   Size:        783    4677
 
XhoI C'TCGA_G

Cuts at:      0    1952    5460 
   Size:       1952    3508
 
XmnI GAAnn'nnTTC

Cuts at:      0     379     383    3620    5067    5460 
   Size:        379       4    3237    1447     393

  Fragments arranged by size: 

                3237    1447     393     379       4
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AleI    AlwNI     ApoI     AseI
     AvaI    BanII     BbsI    BbvCI     BclI    BfrBI    BglII     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI
    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I
     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII
  HindIII     HpaI     MfeI     MmeI     MscI     MslI   MspA1I     NdeI
     NheI     NsiI     NspI     PciI    PflMI     PfoI     PsiI    PvuII
     SacI     SapI     SfcI     SmlI    SnaBI     SphI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AlfI
     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI
     BanI     BbeI     BcgI    BciVI     BglI     BlpI Bme1580I    BmgBI
     BsaI    BsaHI    BsaWI    BsaXI    BsiWI    BsmBI    BspEI    BspMI
    BsrBI    BsrFI   BssHII   BstEII     BtgI    BtgZI     ClaI     EagI
    EcoNI EcoO109I     FseI     FspI    FspAI     KasI     KpnI     MluI
     NaeI     NarI     NcoI   NgoMIV     NotI     NruI     PacI     PasI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SpeI     SrfI
     XmaI     ZraI
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