################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AccI AclI AflII AgeI AhdI AscI AseI AsnI AspEI AvrII BamHI BclI BfrI BlnI BsaI BsiWI BspHI BsrFI BssHII Bst1107I BstBI BstXI BstZ17I Cfr10I CpoI DraI DraIII Eam1105I EcoRI EcoRV FbaI FseI HincII HindII HpaI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 1367 GGTACC G^GTACC AccIII 2177 TCCGGA T^CCGGA AcyI 1625 GGCGCC G[GA]^CG[CT]C AflIII 2462 ACATGT A^C[GA][CT]GT Alw44I 313 GTGCAC G^TGCAC Aor51HI 2880 AGCGCT AGC^GCT ApaLI 313 GTGCAC G^TGCAC Asp700I 2460 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1367 GGTACC G^GTACC AspI 2509 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2288 TGCGCA TGC^GCA BbeI 1625 GGCGCC GGCGC^C BbvCI 1876 GCTGAGG CCTCAGC(-5/-2) BcgI 1820 GCAAATCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1230 GGATAC GTATCC(6/5) BglI 2260 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 2265 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1625 GGCGCC G[GA]^CG[CT]C BseAI 2177 TCCGGA T^CCGGA BsiEI 4 CGGCCG CG[GA][CT]^CG BsmBI 792 CGTCTC CGTCTC(1/5) Bsp1407I 1343 TGTACA T^GTACA BspDI 556 ATCGAT AT^CGAT BspEI 2177 TCCGGA T^CCGGA BspLU11I 2462 ACATGT A^CATGT BsrGI 1343 TGTACA T^GTACA BstEII 1692 GGTCACC G^GT[ACGT]ACC BstPI 1692 GGTCACC G^GT[ACGT]ACC ClaI 556 ATCGAT AT^CGAT DrdI 484 GACGCTCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 4 CGGCCG C^GGCCG EclXI 4 CGGCCG C^GGCCG Eco47III 2880 AGCGCT AGC^GCT Eco52I 4 CGGCCG C^GGCCG EcoO65I 1692 GGTCACC G^GT[ACGT]ACC EcoT22I 2640 ATGCAT ATGCA^T FspI 2288 TGCGCA TGC^GCA Hin1I 1625 GGCGCC G[GA]^CG[CT]C HindIII 1683 AAGCTT A^AGCTT KasI 1625 GGCGCC G^GCGCC KpnI 1367 GGTACC GGTAC^C KspI 2873 CCGCGG CCGC^GG MamI 2265 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2177 TCCGGA T^CCGGA NarI 1625 GGCGCC GG^CGCC NcoI 1889 CCATGG C^CATGG NsiI 2640 ATGCAT ATGCA^T PflFI 2509 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC SacII 2873 CCGCGG CCGC^GG SfoI 1625 GGCGCC GGC^GCC SphI 2642 GCATGC GCATG^C SspBI 1343 TGTACA T^GTACA Tth111I 2509 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 2460 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 451 AAATTC [GA]^AATT[CT] 1771 GAATTT AocI 52 CCTGAGG CC^T[ACGT]AGG 1103 CCTTAGG ApaI 2078 GGGCCC GGGCC^C 2590 GGGCCC ApoI 451 AAATTC [GA]^AATT[CT] 1771 GAATTT AvaI 427 CTCGGG C^[CT]CG[GA]G 995 CCCGAG BbrPI 292 CACGTG CAC^GTG 1721 CACGTG BglII 733 AGATCT A^GATCT 2348 AGATCT BsaAI 292 CACGTG [CT]AC^GT[GA] 1721 CACGTG BsmAI 793 GTCTC GTCTC(1/5) 838 GTCTC BsoBI 427 CTCGGG C^[CT]CG[GA]G 995 CCCGAG BstAPI 381 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2642 GCATGCTCTGC Bsu36I 52 CCTGAGG CC^T[ACGT]AGG 1103 CCTTAGG Eco81I 52 CCTGAGG CC^T[ACGT]AGG 1103 CCTTAGG HgaI 484 GACGC GACGC(5/10) 562 GCGTC PflMI 134 CCAACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 748 CCACTCTCTGG PmaCI 292 CACGTG CAC^GTG 1721 CACGTG PmlI 292 CACGTG CAC^GTG 1721 CACGTG SmlI 773 CTCAAG C^T[CT][GA]AG 1812 CTCAAG TaqI 557 TCGA T^CGA 1517 TCGA TthHB8I 557 TCGA T^CGA 1517 TCGA Van91I 134 CCAACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 748 CCACTCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmFI BsmI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII