Restriction map for
pF1KE2589
(Linear) (Six-base) MAPSORT of: KIEE2589.seq Check: 175 from: 1 to: 2916
KIEE2589.orf
With 182 enzymes: SgfI *
October 20, 2015 16:07 ..
Acc65I G'GTAC_C
Cuts at: 0 1367 2916
Size: 1367 1549
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1470 1624 1921 2286 2916
Size: 1470 154 297 365 630
Fragments arranged by size:
1470 630 365 297 154
AfeI AGC'GCT
Cuts at: 0 2882 2916
Size: 2882 34
AflIII A'CryG_T
Cuts at: 0 2462 2916
Size: 2462 454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 196 2916
Size: 196 2720
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1375 1407 2916
Size: 1375 32 1509
Fragments arranged by size:
1509 1375 32
AlwNI CAG_nnn'CTG
Cuts at: 0 84 466 513 618 841 1090 1602 2838
Size: 84 382 47 105 223 249 512 1236
Cuts at: 2838 2916
Size: 78
Fragments arranged by size:
1236 512 382 249 223 105 84 78
47
ApaI G_GGCC'C
Cuts at: 0 2082 2594 2916
Size: 2082 512 322
Fragments arranged by size:
2082 512 322
ApaLI G'TGCA_C
Cuts at: 0 313 2916
Size: 313 2603
ApoI r'AATT_y
Cuts at: 0 451 1771 2916
Size: 451 1320 1145
Fragments arranged by size:
1320 1145 451
AvaI C'yCGr_G
Cuts at: 0 427 995 2916
Size: 427 568 1921
Fragments arranged by size:
1921 568 427
BanI G'GyrC_C
Cuts at: 0 343 1367 1625 2916
Size: 343 1024 258 1291
Fragments arranged by size:
1291 1024 343 258
BanII G_rGCy'C
Cuts at: 0 29 592 886 1257 1898 2082 2594 2609
Size: 29 563 294 371 641 184 512 15
Cuts at: 2609 2717 2873 2916
Size: 108 156 43
Fragments arranged by size:
641 563 512 371 294 184 156 108
43 29 15
BbeI G_GCGC'C
Cuts at: 0 1629 2916
Size: 1629 1287
BbsI GAAGACnn'nnnn_
Cuts at: 0 103 1404 1583 2916
Size: 103 1301 179 1333
Fragments arranged by size:
1333 1301 179 103
BbvCI CC'TCA_GC
Cuts at: 0 1877 2916
Size: 1877 1039
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1809 1843 2916
Size: 1809 34 1073
Fragments arranged by size:
1809 1073 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1224 2916
Size: 1224 1692
BfrBI ATG'CAT
Cuts at: 0 2642 2916
Size: 2642 274
BglI GCCn_nnn'nGGC
Cuts at: 0 2266 2916
Size: 2266 650
BglII A'GATC_T
Cuts at: 0 733 2348 2916
Size: 733 1615 568
Fragments arranged by size:
1615 733 568
BlpI GC'TnA_GC
Cuts at: 0 665 1673 2603 2916
Size: 665 1008 930 313
Fragments arranged by size:
1008 930 665 313
Bme1580I G_kGCm'C
Cuts at: 0 317 713 791 2082 2394 2594 2916
Size: 317 396 78 1291 312 200 322
Fragments arranged by size:
1291 396 322 317 312 200 78
BmgBI CAC'GTC
Cuts at: 0 792 1871 2916
Size: 792 1079 1045
Fragments arranged by size:
1079 1045 792
BmrI ACTGGGnnnn_n'
Cuts at: 0 626 823 1704 1833 2916
Size: 626 197 881 129 1083
Fragments arranged by size:
1083 881 626 197 129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 870 1050 1230 1241 1683 1963 2860 2916
Size: 870 180 180 11 442 280 897 56
Fragments arranged by size:
897 870 442 280 180 180 56 11
Bpu10I CC'TnA_GC
Cuts at: 0 1377 1877 2916
Size: 1377 500 1039
Fragments arranged by size:
1377 1039 500
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 1797 2916
Size: 758 1039 1119
Fragments arranged by size:
1119 1039 758
BsaAI yAC'GTr
Cuts at: 0 294 1723 2916
Size: 294 1429 1193
Fragments arranged by size:
1429 1193 294
BsaBI GATnn'nnATC
Cuts at: 0 2269 2916
Size: 2269 647
BsaHI Gr'CG_yC
Cuts at: 0 1626 2916
Size: 1626 1290
BsaWI w'CCGG_w
Cuts at: 0 263 2013 2177 2916
Size: 263 1750 164 739
Fragments arranged by size:
1750 739 263 164
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1204 1234 1930 1960 2916
Size: 1204 30 696 30 956
Fragments arranged by size:
1204 956 696 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 70 1053 1570 1573 2878 2881 2916
Size: 55 15 983 517 3 1305 3 35
Fragments arranged by size:
1305 983 517 55 35 15 3 3
BseYI C'CCAG_C
Cuts at: 0 84 410 1333 2275 2586 2916
Size: 84 326 923 942 311 330
Fragments arranged by size:
942 923 330 326 311 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 1846 2183 2395 2916
Size: 827 1019 337 212 521
Fragments arranged by size:
1019 827 521 337 212
BsiEI CG_ry'CG
Cuts at: 0 7 2916
Size: 7 2909
BsiHKAI G_wGCw'C
Cuts at: 0 29 74 317 886 1257 1378 1679 2609
Size: 29 45 243 569 371 121 301 930
Cuts at: 2609 2717 2916
Size: 108 199
Fragments arranged by size:
930 569 371 301 243 199 121 108
45 29
BsmI GAATG_Cn'
Cuts at: 0 669 1482 2262 2916
Size: 669 813 780 654
Fragments arranged by size:
813 780 669 654
BsmBI CGTCTCn'nnnn_
Cuts at: 0 798 2916
Size: 798 2118
Bsp1286I G_dGCh'C
Cuts at: 0 29 74 317 592 713 791 886 1257
Size: 29 45 243 275 121 78 95 371
Cuts at: 1257 1378 1679 1898 2082 2394 2594 2609 2717
Size: 121 301 219 184 312 200 15 108
Cuts at: 2717 2873 2916
Size: 156 43
Fragments arranged by size:
371 312 301 275 243 219 200 184
156 121 121 108 95 78 45 43
29 15
BspEI T'CCGG_A
Cuts at: 0 2177 2916
Size: 2177 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1145 1658 2345 2916
Size: 1145 513 687 571
Fragments arranged by size:
1145 687 571 513
BsrBI CCG'CTC
Cuts at: 0 165 594 2799 2916
Size: 165 429 2205 117
Fragments arranged by size:
2205 429 165 117
BsrDI GCAATG_nn'
Cuts at: 0 1038 1238 1275 2916
Size: 1038 200 37 1641
Fragments arranged by size:
1641 1038 200 37
BsrGI T'GTAC_A
Cuts at: 0 1343 2916
Size: 1343 1573
BssSI C'ACGA_G
Cuts at: 0 316 583 2251 2916
Size: 316 267 1668 665
Fragments arranged by size:
1668 665 316 267
BstAPI GCAn_nnn'nTGC
Cuts at: 0 387 2648 2916
Size: 387 2261 268
Fragments arranged by size:
2261 387 268
BstEII G'GTnAC_C
Cuts at: 0 1692 2916
Size: 1692 1224
BstYI r'GATC_y
Cuts at: 0 733 1592 1613 2348 2916
Size: 733 859 21 735 568
Fragments arranged by size:
859 735 733 568 21
Bsu36I CC'TnA_GG
Cuts at: 0 53 1104 2916
Size: 53 1051 1812
Fragments arranged by size:
1812 1051 53
BtgI C'CryG_G
Cuts at: 0 1889 2138 2873 2916
Size: 1889 249 735 43
Fragments arranged by size:
1889 735 249 43
BtsI GCAGTG_nn'
Cuts at: 0 339 396 514 527 1323 1851 1999 2916
Size: 339 57 118 13 796 528 148 917
Fragments arranged by size:
917 796 528 339 148 118 57 13
ClaI AT'CG_AT
Cuts at: 0 557 2916
Size: 557 2359
DrdI GACnn_nn'nnGTC
Cuts at: 0 490 2916
Size: 490 2426
EaeI y'GGCC_r
Cuts at: 0 4 497 974 1886 2141 2267 2483 2825
Size: 4 493 477 912 255 126 216 342
Cuts at: 2825 2916
Size: 91
Fragments arranged by size:
912 493 477 342 255 216 126 91
4
EagI C'GGCC_G
Cuts at: 0 4 2916
Size: 4 2912
EarI CTCTTCn'nnn_
Cuts at: 0 1104 1565 2371 2732 2916
Size: 1104 461 806 361 184
Fragments arranged by size:
1104 806 461 361 184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1606 2916
Size: 1606 1310
EcoICRI GAG'CTC
Cuts at: 0 27 884 1255 2607 2715 2916
Size: 27 857 371 1352 108 201
Fragments arranged by size:
1352 857 371 201 108 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 870 1050 1230 1241 1470 1624 1683 1921
Size: 870 180 180 11 229 154 59 238
Cuts at: 1921 1963 2286 2860 2916
Size: 42 323 574 56
Fragments arranged by size:
870 574 323 238 229 180 180 154
59 56 42 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 1468 1555 2530 2916
Size: 52 1416 87 975 386
Fragments arranged by size:
1416 975 386 87 52
EcoO109I rG'GnC_Cy
Cuts at: 0 683 961 1291 2079 2334 2535 2590 2591
Size: 683 278 330 788 255 201 55 1
Cuts at: 2591 2916
Size: 325
Fragments arranged by size:
788 683 330 325 278 255 201 55
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 755 2316 2916
Size: 755 1561 600
Fragments arranged by size:
1561 755 600
FspI TGC'GCA
Cuts at: 0 2290 2916
Size: 2290 626
HaeII r_GCGC'y
Cuts at: 0 48 1629 2884 2916
Size: 48 1581 1255 32
Fragments arranged by size:
1581 1255 48 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 428 460 718 750 776 808 1403 1435
Size: 428 32 258 32 26 32 595 32
Cuts at: 1435 1735 1767 2159 2191 2630 2662 2916
Size: 300 32 392 32 439 32 254
Fragments arranged by size:
595 439 428 392 300 258 254 32
32 32 32 32 32 32 26
HindIII A'AGCT_T
Cuts at: 0 1683 2916
Size: 1683 1233
KasI G'GCGC_C
Cuts at: 0 1625 2916
Size: 1625 1291
KpnI G_GTAC'C
Cuts at: 0 1371 2916
Size: 1371 1545
MscI TGG'CCA
Cuts at: 0 976 1888 2143 2269 2485 2827 2916
Size: 976 912 255 126 216 342 89
Fragments arranged by size:
976 912 342 255 216 126 89
MslI CAynn'nnrTG
Cuts at: 0 310 499 2255 2916
Size: 310 189 1756 661
Fragments arranged by size:
1756 661 310 189
MspA1I CmG'CkG
Cuts at: 0 1013 1137 1454 2159 2445 2875 2916
Size: 1013 124 317 705 286 430 41
Fragments arranged by size:
1013 705 430 317 286 124 41
NarI GG'CG_CC
Cuts at: 0 1626 2916
Size: 1626 1290
NcoI C'CATG_G
Cuts at: 0 1889 2916
Size: 1889 1027
NsiI A_TGCA'T
Cuts at: 0 2644 2916
Size: 2644 272
NspI r_CATG'y
Cuts at: 0 183 1392 2466 2646 2916
Size: 183 1209 1074 180 270
Fragments arranged by size:
1209 1074 270 183 180
PciI A'CATG_T
Cuts at: 0 2462 2916
Size: 2462 454
PflMI CCAn_nnn'nTGG
Cuts at: 0 140 754 2916
Size: 140 614 2162
Fragments arranged by size:
2162 614 140
PfoI T'CCnGG_A
Cuts at: 0 1595 1955 2903 2916
Size: 1595 360 948 13
Fragments arranged by size:
1595 948 360 13
PmlI CAC'GTG
Cuts at: 0 294 1723 2916
Size: 294 1429 1193
Fragments arranged by size:
1429 1193 294
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1032 1064 1795 1827 2916
Size: 1032 32 731 32 1089
Fragments arranged by size:
1089 1032 731 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 683 2334 2535 2916
Size: 683 1651 201 381
Fragments arranged by size:
1651 683 381 201
PspOMI G'GGCC_C
Cuts at: 0 2078 2590 2916
Size: 2078 512 326
Fragments arranged by size:
2078 512 326
PstI C_TGCA'G
Cuts at: 0 380 392 509 2807 2916
Size: 380 12 117 2298 109
Fragments arranged by size:
2298 380 117 109 12
PvuII CAG'CTG
Cuts at: 0 1013 1137 1454 2445 2916
Size: 1013 124 317 991 471
Fragments arranged by size:
1013 991 471 317 124
SacI G_AGCT'C
Cuts at: 0 29 886 1257 2609 2717 2916
Size: 29 857 371 1352 108 199
Fragments arranged by size:
1352 857 371 199 108 29
SacII CC_GC'GG
Cuts at: 0 2876 2916
Size: 2876 40
ScaI AGT'ACT
Cuts at: 0 891 2233 2311 2916
Size: 891 1342 78 605
Fragments arranged by size:
1342 891 605 78
SfcI C'TryA_G
Cuts at: 0 376 388 505 2397 2803 2916
Size: 376 12 117 1892 406 113
Fragments arranged by size:
1892 406 376 117 113 12
SfoI GGC'GCC
Cuts at: 0 1627 2916
Size: 1627 1289
SmlI C'TyrA_G
Cuts at: 0 773 1812 2916
Size: 773 1039 1104
Fragments arranged by size:
1104 1039 773
SphI G_CATG'C
Cuts at: 0 2646 2916
Size: 2646 270
StyI C'CwwG_G
Cuts at: 0 1889 2330 2486 2916
Size: 1889 441 156 430
Fragments arranged by size:
1889 441 430 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1347 2916
Size: 1347 1569
TatI w'GTAC_w
Cuts at: 0 889 1343 2231 2309 2546 2916
Size: 889 454 888 78 237 370
Fragments arranged by size:
889 888 454 370 237 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1204 1236 1930 1962 2916
Size: 1204 32 694 32 954
Fragments arranged by size:
1204 954 694 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2512 2916
Size: 2512 404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 984 1120 2493 2835 2916
Size: 984 136 1373 342 81
Fragments arranged by size:
1373 984 342 136 81
XmnI GAAnn'nnTTC
Cuts at: 0 2464 2916
Size: 2464 452
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrGI BssSI BstAPI
BstEII BstYI Bsu36I BtgI BtsI ClaI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HindIII KasI KpnI MscI MslI
MspA1I NarI NcoI NsiI NspI PciI PflMI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII
ScaI SfcI SfoI SmlI SphI StyI TaqII TatI
TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AleI
AscI AseI AsiSI AvrII BaeI BamHI BclI BmtI
BplI BsaI BsiWI BspHI BsrFI BssHII BstBI BstXI
BstZ17I BtgZI CspCI DraI DraIII EcoRI EcoRV FseI
FspAI HincII HpaI MfeI MluI MmeI NaeI NdeI
NgoMIV NheI NotI NruI PacI PasI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI
SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI XbaI XhoI XmaI ZraI