(Circular) (Six-base) MAPSORT of: pF1KE2600.seq Check: 8123 from: 1 to: 4004 pF1KE2600 4004 bp With 182 enzymes: SgfI * January 12, 2016 10:05 .. AccI GT'mk_AC Cuts at: 1042 1042 Size: 4004 Acc65I G'GTAC_C Cuts at: 1020 1020 Size: 4004 AclI AA'CG_TT Cuts at: 3844 3844 Size: 4004 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 817 1829 2261 3008 3276 817 Size: 1012 432 747 268 1545 Fragments arranged by size: 1545 1012 747 432 268 AfeI AGC'GCT Cuts at: 367 679 367 Size: 312 3692 AflIII A'CryG_T Cuts at: 1356 2460 3437 1356 Size: 1104 977 1923 Fragments arranged by size: 1923 1104 977 AgeI A'CCGG_T Cuts at: 2392 2392 Size: 4004 AhdI GACnn_n'nnGTC Cuts at: 696 696 Size: 4004 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 712 744 712 Size: 32 3972 AlwNI CAG_nnn'CTG Cuts at: 426 1309 2876 426 Size: 883 1567 1554 Fragments arranged by size: 1567 1554 883 ApaLI G'TGCA_C Cuts at: 245 973 2774 245 Size: 728 1801 1475 Fragments arranged by size: 1801 1475 728 ApoI r'AATT_y Cuts at: 1008 3357 3613 1008 Size: 2349 256 1399 Fragments arranged by size: 2349 1399 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4004 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4004 AvaI C'yCGr_G Cuts at: 198 1024 198 Size: 826 3178 BamHI G'GATC_C Cuts at: 1029 1029 Size: 4004 BanI G'GyrC_C Cuts at: 1020 1434 1682 1717 1020 Size: 414 248 35 3307 Fragments arranged by size: 3307 414 248 35 BanII G_rGCy'C Cuts at: 199 446 580 1018 2048 199 Size: 247 134 438 1030 2155 Fragments arranged by size: 2155 1030 438 247 134 BbeI G_GCGC'C Cuts at: 1438 1686 1438 Size: 248 3756 BbsI GAAGACnn'nnnn_ Cuts at: 557 785 557 Size: 228 3776 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 226 260 90 Size: 34 102 34 3834 Fragments arranged by size: 3834 102 34 34 BciVI GTATCCnnnnn_n' Cuts at: 1902 2663 4004 1902 Size: 761 1341 1902 Fragments arranged by size: 1902 1341 761 BclI T'GATC_A Cuts at: 1524 1524 Size: 4004 BglII A'GATC_T Cuts at: 1519 3556 1519 Size: 2037 1967 BlpI GC'TnA_GC Cuts at: 1116 1116 Size: 4004 Bme1580I G_kGCm'C Cuts at: 249 977 1629 1722 2778 249 Size: 728 652 93 1056 1475 Fragments arranged by size: 1475 1056 728 652 93 BmgBI CAC'GTC Cuts at: 323 972 323 Size: 649 3355 BmrI ACTGGGnnnn_n' Cuts at: 249 1216 1297 1632 3776 249 Size: 967 81 335 2144 477 Fragments arranged by size: 2144 967 477 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2371 2371 Size: 4004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 491 573 491 Size: 82 3922 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1180 2059 2551 2849 1180 Size: 879 492 298 2335 Fragments arranged by size: 2335 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3708 3708 Size: 4004 BsaAI yAC'GTr Cuts at: 1357 1357 Size: 4004 BsaBI GATnn'nnATC Cuts at: 6 643 1518 3555 6 Size: 637 875 2037 455 Fragments arranged by size: 2037 875 637 455 BsaHI Gr'CG_yC Cuts at: 1435 1683 2385 3881 1435 Size: 248 702 1496 1558 Fragments arranged by size: 1558 1496 702 248 BsaWI w'CCGG_w Cuts at: 1193 1714 2392 2666 2813 3224 1193 Size: 521 678 274 147 411 1973 Fragments arranged by size: 1973 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 565 595 712 742 565 Size: 30 117 30 3827 Fragments arranged by size: 3827 117 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 717 717 Size: 4004 BseYI C'CCAG_C Cuts at: 1429 2187 2764 3260 1429 Size: 758 577 496 2173 Fragments arranged by size: 2173 758 577 496 BsiEI CG_ry'CG Cuts at: 104 415 438 1546 1592 2800 104 Size: 311 23 1108 46 1208 1308 Fragments arranged by size: 1308 1208 1108 311 46 23 BsiHKAI G_wGCw'C Cuts at: 199 249 709 977 1018 1796 1986 2778 199 Size: 50 460 268 41 778 190 792 1425 Fragments arranged by size: 1425 792 778 460 268 190 50 41 BsmBI CGTCTCn'nnnn_ Cuts at: 591 591 Size: 4004 Bsp1286I G_dGCh'C Cuts at: 199 249 446 580 709 977 1018 1629 1722 Size: 50 197 134 129 268 41 611 93 Cuts at: 1722 1796 1986 2048 2778 199 Size: 74 190 62 730 1425 Fragments arranged by size: 1425 730 611 268 197 190 134 129 93 74 62 50 41 BspEI T'CCGG_A Cuts at: 3224 3224 Size: 4004 BspHI T'CATG_A Cuts at: 3180 3180 Size: 4004 BspMI ACCTGCnnnn'nnnn_ Cuts at: 494 1054 1268 1570 1951 494 Size: 560 214 302 381 2547 Fragments arranged by size: 2547 560 381 302 214 BsrBI CCG'CTC Cuts at: 50 2296 2350 3834 3999 50 Size: 2246 54 1484 165 55 Fragments arranged by size: 2246 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1916 1916 Size: 4004 BsrFI r'CCGG_y Cuts at: 174 562 2002 2183 2392 174 Size: 388 1440 181 209 1786 Fragments arranged by size: 1786 1440 388 209 181 BsrGI T'GTAC_A Cuts at: 828 828 Size: 4004 BssHII G'CGCG_C Cuts at: 260 477 260 Size: 217 3787 BssSI C'ACGA_G Cuts at: 2275 2633 2275 Size: 358 3646 BstAPI GCAn_nnn'nTGC Cuts at: 426 1381 426 Size: 955 3049 BstBI TT'CG_AA Cuts at: 2365 2365 Size: 4004 BstYI r'GATC_y Cuts at: 1 1029 1496 1519 1854 2100 3112 3198 3210 Size: 1028 467 23 335 246 1012 86 12 Cuts at: 3210 3556 1 Size: 346 449 Fragments arranged by size: 1028 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 165 215 438 165 Size: 50 223 3731 Fragments arranged by size: 3731 223 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1954 2135 2298 1954 Size: 181 163 3660 Fragments arranged by size: 3660 181 163 BtsI GCAGTG_nn' Cuts at: 573 880 975 1247 573 Size: 307 95 272 3330 Fragments arranged by size: 3330 307 272 95 DraI TTT'AAA Cuts at: 1003 1003 Size: 4004 DraIII CAC_nnn'GTG Cuts at: 603 603 Size: 4004 DrdI GACnn_nn'nnGTC Cuts at: 1710 2568 1710 Size: 858 3146 EaeI y'GGCC_r Cuts at: 435 1589 1763 2154 2181 3526 435 Size: 1154 174 391 27 1345 913 Fragments arranged by size: 1345 1154 913 391 174 27 EagI C'GGCC_G Cuts at: 435 1589 435 Size: 1154 2850 EarI CTCTTCn'nnn_ Cuts at: 2027 2237 2027 Size: 210 3794 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 257 2212 2522 2668 3814 257 Size: 1955 310 146 1146 447 Fragments arranged by size: 1955 1146 447 310 146 EcoICRI GAG'CTC Cuts at: 197 1016 197 Size: 819 3185 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 491 573 817 1829 2261 3008 3276 491 Size: 82 244 1012 432 747 268 1219 Fragments arranged by size: 1219 1012 747 432 268 244 82 EcoNI CCTnn'n_nnAGG Cuts at: 887 941 887 Size: 54 3950 EcoO109I rG'GnC_Cy Cuts at: 211 1143 211 Size: 932 3072 EcoRI G'AATT_C Cuts at: 1008 1008 Size: 4004 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 921 2023 921 Size: 1102 2902 FspI TGC'GCA Cuts at: 327 1785 327 Size: 1458 2546 FspAI rTGC'GCAy Cuts at: 327 327 Size: 4004 HaeII r_GCGC'y Cuts at: 369 427 499 563 681 1438 1686 2708 3687 Size: 58 72 64 118 757 248 1022 979 Cuts at: 3687 369 Size: 686 Fragments arranged by size: 1022 979 757 686 248 118 72 64 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 113 145 565 597 682 714 1846 1876 1878 Size: 32 420 32 85 32 1132 30 2 Cuts at: 1878 1908 113 Size: 30 2209 Fragments arranged by size: 2209 1132 420 85 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 453 690 1043 453 Size: 237 353 3414 Fragments arranged by size: 3414 353 237 KasI G'GCGC_C Cuts at: 1434 1682 1434 Size: 248 3756 KpnI G_GTAC'C Cuts at: 1024 1024 Size: 4004 MluI A'CGCG_T Cuts at: 3437 3437 Size: 4004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 524 2675 2859 524 Size: 2151 184 1669 Fragments arranged by size: 2151 1669 184 MscI TGG'CCA Cuts at: 1765 1765 Size: 4004 MslI CAynn'nnrTG Cuts at: 1993 2120 1993 Size: 127 3877 MspA1I CmG'CkG Cuts at: 114 139 167 417 440 1115 1429 1789 2802 Size: 25 28 250 23 675 314 360 1013 Cuts at: 2802 3047 114 Size: 245 1071 Fragments arranged by size: 1071 1013 675 360 314 250 245 28 25 23 NaeI GCC'GGC Cuts at: 564 2185 564 Size: 1621 2383 NarI GG'CG_CC Cuts at: 1435 1683 1435 Size: 248 3756 NgoMIV G'CCGG_C Cuts at: 562 2183 562 Size: 1621 2383 NspI r_CATG'y Cuts at: 664 990 1057 1557 2464 664 Size: 326 67 500 907 2204 Fragments arranged by size: 2204 907 500 326 67 PasI CC'CwG_GG Cuts at: 821 821 Size: 4004 PciI A'CATG_T Cuts at: 2460 2460 Size: 4004 PflMI CCAn_nnn'nTGG Cuts at: 131 131 Size: 4004 PmeI GTTT'AAAC Cuts at: 1003 1003 Size: 4004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 712 744 3143 3175 712 Size: 32 2399 32 1541 Fragments arranged by size: 2399 1541 32 32 PpuMI rG'GwC_Cy Cuts at: 211 211 Size: 4004 PsiI TTA'TAA Cuts at: 863 863 Size: 4004 PstI C_TGCA'G Cuts at: 422 1051 1736 422 Size: 629 685 2690 Fragments arranged by size: 2690 685 629 PvuI CG_AT'CG Cuts at: 104 104 Size: 4004 PvuII CAG'CTG Cuts at: 1429 1789 1429 Size: 360 3644 RsrII CG'GwC_CG Cuts at: 2199 2199 Size: 4004 SacI G_AGCT'C Cuts at: 199 1018 199 Size: 819 3185 SacII CC_GC'GG Cuts at: 168 441 168 Size: 273 3731 SalI G'TCGA_C Cuts at: 1041 1041 Size: 4004 SanDI GG'GwC_CC Cuts at: 211 211 Size: 4004 SapI GCTCTTCn'nnn_ Cuts at: 2027 2237 2027 Size: 210 3794 SbfI CC_TGCA'GG Cuts at: 1051 1051 Size: 4004 ScaI AGT'ACT Cuts at: 3521 3521 Size: 4004 SfcI C'TryA_G Cuts at: 33 418 981 1047 1732 2725 2916 33 Size: 385 563 66 685 993 191 1121 Fragments arranged by size: 1121 993 685 563 385 191 66 SfoI GGC'GCC Cuts at: 1436 1684 1436 Size: 248 3756 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4004 SgrAI Cr'CCGG_yG Cuts at: 174 174 Size: 4004 SmaI CCC'GGG Cuts at: 1026 1026 Size: 4004 SmlI C'TyrA_G Cuts at: 1159 2074 2566 2828 1159 Size: 915 492 262 2335 Fragments arranged by size: 2335 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4004 SphI G_CATG'C Cuts at: 664 990 1057 1557 664 Size: 326 67 500 3111 Fragments arranged by size: 3111 500 326 67 StyI C'CwwG_G Cuts at: 956 1138 1492 956 Size: 182 354 3468 Fragments arranged by size: 3468 354 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1587 1720 2179 2388 1587 Size: 133 459 209 3203 Fragments arranged by size: 3203 459 209 133 TatI w'GTAC_w Cuts at: 828 3519 828 Size: 2691 1313 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3521 3553 3521 Size: 32 3972 Tth111I GACn'n_nGTC Cuts at: 1801 1801 Size: 4004 XbaI T'CTAG_A Cuts at: 1035 1035 Size: 4004 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1499 1499 Size: 4004 XmaI C'CCGG_G Cuts at: 1024 1024 Size: 4004 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AleI AlfI ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I BsgI BsiWI BsmI BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PfoI PmlI PshAI PspOMI PspXI PsrI SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI