(Linear) (Six-base) MAPSORT of: KIEE2600.seq Check: 10 from: 1 to: 891 KIEE2600.orf With 182 enzymes: SgfI * January 12, 2016 10:05 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 709 891 Size: 709 182 AfeI AGC'GCT Cuts at: 0 259 571 891 Size: 259 312 320 Fragments arranged by size: 320 312 259 AhdI GACnn_n'nnGTC Cuts at: 0 588 891 Size: 588 303 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 604 636 891 Size: 604 32 255 Fragments arranged by size: 604 255 32 AlwNI CAG_nnn'CTG Cuts at: 0 318 891 Size: 318 573 ApaLI G'TGCA_C Cuts at: 0 137 865 891 Size: 137 728 26 Fragments arranged by size: 728 137 26 AvaI C'yCGr_G Cuts at: 0 90 891 Size: 90 801 BanII G_rGCy'C Cuts at: 0 91 338 472 891 Size: 91 247 134 419 Fragments arranged by size: 419 247 134 91 BbsI GAAGACnn'nnnn_ Cuts at: 0 449 677 891 Size: 449 228 214 Fragments arranged by size: 449 228 214 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 118 152 891 Size: 118 34 739 Fragments arranged by size: 739 118 34 Bme1580I G_kGCm'C Cuts at: 0 141 869 891 Size: 141 728 22 Fragments arranged by size: 728 141 22 BmgBI CAC'GTC Cuts at: 0 215 864 891 Size: 215 649 27 Fragments arranged by size: 649 215 27 BmrI ACTGGGnnnn_n' Cuts at: 0 141 891 Size: 141 750 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 383 465 891 Size: 383 82 426 Fragments arranged by size: 426 383 82 BsaBI GATnn'nnATC Cuts at: 0 535 891 Size: 535 356 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 457 487 604 634 891 Size: 457 30 117 30 257 Fragments arranged by size: 457 257 117 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 609 891 Size: 609 282 BsiEI CG_ry'CG Cuts at: 0 307 330 891 Size: 307 23 561 Fragments arranged by size: 561 307 23 BsiHKAI G_wGCw'C Cuts at: 0 91 141 601 869 891 Size: 91 50 460 268 22 Fragments arranged by size: 460 268 91 50 22 BsmBI CGTCTCn'nnnn_ Cuts at: 0 483 891 Size: 483 408 Bsp1286I G_dGCh'C Cuts at: 0 91 141 338 472 601 869 891 Size: 91 50 197 134 129 268 22 Fragments arranged by size: 268 197 134 129 91 50 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 386 891 Size: 386 505 BsrFI r'CCGG_y Cuts at: 0 66 454 891 Size: 66 388 437 Fragments arranged by size: 437 388 66 BsrGI T'GTAC_A Cuts at: 0 720 891 Size: 720 171 BssHII G'CGCG_C Cuts at: 0 152 369 891 Size: 152 217 522 Fragments arranged by size: 522 217 152 BstAPI GCAn_nnn'nTGC Cuts at: 0 318 891 Size: 318 573 BtgI C'CryG_G Cuts at: 0 57 107 330 891 Size: 57 50 223 561 Fragments arranged by size: 561 223 57 50 BtsI GCAGTG_nn' Cuts at: 0 465 772 867 891 Size: 465 307 95 24 Fragments arranged by size: 465 307 95 24 DraIII CAC_nnn'GTG Cuts at: 0 495 891 Size: 495 396 EaeI y'GGCC_r Cuts at: 0 327 891 Size: 327 564 EagI C'GGCC_G Cuts at: 0 327 891 Size: 327 564 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 149 891 Size: 149 742 EcoICRI GAG'CTC Cuts at: 0 89 891 Size: 89 802 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 383 465 709 891 Size: 383 82 244 182 Fragments arranged by size: 383 244 182 82 EcoNI CCTnn'n_nnAGG Cuts at: 0 779 833 891 Size: 779 54 58 Fragments arranged by size: 779 58 54 EcoO109I rG'GnC_Cy Cuts at: 0 103 891 Size: 103 788 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 813 891 Size: 813 78 FspI TGC'GCA Cuts at: 0 219 891 Size: 219 672 FspAI rTGC'GCAy Cuts at: 0 219 891 Size: 219 672 HaeII r_GCGC'y Cuts at: 0 261 319 391 455 573 891 Size: 261 58 72 64 118 318 Fragments arranged by size: 318 261 118 72 64 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 5 37 457 489 574 606 891 Size: 5 32 420 32 85 32 285 Fragments arranged by size: 420 285 85 32 32 32 5 HincII GTy'rAC Cuts at: 0 345 582 891 Size: 345 237 309 Fragments arranged by size: 345 309 237 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 416 891 Size: 416 475 MspA1I CmG'CkG Cuts at: 0 6 31 59 309 332 891 Size: 6 25 28 250 23 559 Fragments arranged by size: 559 250 28 25 23 6 NaeI GCC'GGC Cuts at: 0 456 891 Size: 456 435 NgoMIV G'CCGG_C Cuts at: 0 454 891 Size: 454 437 NspI r_CATG'y Cuts at: 0 556 882 891 Size: 556 326 9 Fragments arranged by size: 556 326 9 PasI CC'CwG_GG Cuts at: 0 713 891 Size: 713 178 PflMI CCAn_nnn'nTGG Cuts at: 0 23 891 Size: 23 868 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 604 636 891 Size: 604 32 255 Fragments arranged by size: 604 255 32 PpuMI rG'GwC_Cy Cuts at: 0 103 891 Size: 103 788 PsiI TTA'TAA Cuts at: 0 755 891 Size: 755 136 PstI C_TGCA'G Cuts at: 0 314 891 Size: 314 577 SacI G_AGCT'C Cuts at: 0 91 891 Size: 91 800 SacII CC_GC'GG Cuts at: 0 60 333 891 Size: 60 273 558 Fragments arranged by size: 558 273 60 SanDI GG'GwC_CC Cuts at: 0 103 891 Size: 103 788 SfcI C'TryA_G Cuts at: 0 310 873 891 Size: 310 563 18 Fragments arranged by size: 563 310 18 SgrAI Cr'CCGG_yG Cuts at: 0 66 891 Size: 66 825 SphI G_CATG'C Cuts at: 0 556 882 891 Size: 556 326 9 Fragments arranged by size: 556 326 9 StyI C'CwwG_G Cuts at: 0 848 891 Size: 848 43 TatI w'GTAC_w Cuts at: 0 720 891 Size: 720 171 Enzymes that do cut: AcuI AfeI AhdI AloI AlwNI ApaLI AvaI BanII BbsI BcgI Bme1580I BmgBI BmrI BpmI BsaBI BsaXI BseRI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BsrGI BssHII BstAPI BtgI BtsI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HincII MmeI MspA1I NaeI NgoMIV NspI PasI PflMI PpiI PpuMI PsiI PstI SacI SacII SanDI SfcI SgrAI SphI StyI TatI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AleI AlfI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseYI BsgI BsiWI BsmI BspEI BspHI BsrBI BsrDI BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EarI EcoRI EcoRV FseI HindIII HpaI KasI KpnI MfeI MluI MscI MslI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI