(Circular) (Six-base) MAPSORT of: pF1KE2674.seq Check: 8190 from: 1 to: 6056 pF1KE2674 6056 bp With 182 enzymes: SgfI * February 27, 2017 10:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1941 2063 1941 Size: 122 5934 AccI GT'mk_AC Cuts at: 3094 3094 Size: 6056 Acc65I G'GTAC_C Cuts at: 2167 3072 2167 Size: 905 5151 AclI AA'CG_TT Cuts at: 5896 5896 Size: 6056 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 293 2858 3881 4313 5060 5328 293 Size: 2565 1023 432 747 268 1021 Fragments arranged by size: 2565 1023 1021 747 432 268 AflIII A'CryG_T Cuts at: 1933 3408 4512 5489 1933 Size: 1475 1104 977 2500 Fragments arranged by size: 2500 1475 1104 977 AgeI A'CCGG_T Cuts at: 4444 4444 Size: 6056 AleI CACnn'nnGTG Cuts at: 2047 2047 Size: 6056 AlwNI CAG_nnn'CTG Cuts at: 3361 4928 3361 Size: 1567 4489 ApaLI G'TGCA_C Cuts at: 2256 4826 2256 Size: 2570 3486 ApoI r'AATT_y Cuts at: 527 666 1451 1477 3060 5409 5665 527 Size: 139 785 26 1583 2349 256 918 Fragments arranged by size: 2349 1583 918 785 256 139 26 AseI AT'TA_AT Cuts at: 20 718 20 Size: 698 5358 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6056 AvaI C'yCGr_G Cuts at: 329 2325 3076 329 Size: 1996 751 3309 Fragments arranged by size: 3309 1996 751 BamHI G'GATC_C Cuts at: 3081 3081 Size: 6056 BanI G'GyrC_C Cuts at: 2167 3072 3486 3734 3769 2167 Size: 905 414 248 35 4454 Fragments arranged by size: 4454 905 414 248 35 BanII G_rGCy'C Cuts at: 1574 3070 4100 1574 Size: 1496 1030 3530 Fragments arranged by size: 3530 1496 1030 BbeI G_GCGC'C Cuts at: 3490 3738 3490 Size: 248 5808 BbsI GAAGACnn'nnnn_ Cuts at: 2918 2918 Size: 6056 BciVI GTATCCnnnnn_n' Cuts at: 2570 2733 3954 4715 6056 2570 Size: 163 1221 761 1341 2570 Fragments arranged by size: 2570 1341 1221 761 163 BclI T'GATC_A Cuts at: 513 1755 3576 513 Size: 1242 1821 2993 Fragments arranged by size: 2993 1821 1242 BglII A'GATC_T Cuts at: 2103 3571 5608 2103 Size: 1468 2037 2551 Fragments arranged by size: 2551 2037 1468 BlpI GC'TnA_GC Cuts at: 3168 3168 Size: 6056 Bme1580I G_kGCm'C Cuts at: 2260 2604 3681 3774 4830 2260 Size: 344 1077 93 1056 3486 Fragments arranged by size: 3486 1077 1056 344 93 BmgBI CAC'GTC Cuts at: 1134 1134 Size: 6056 BmrI ACTGGGnnnn_n' Cuts at: 2358 2579 3268 3349 3684 5828 2358 Size: 221 689 81 335 2144 2586 Fragments arranged by size: 2586 2144 689 335 221 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4423 4423 Size: 6056 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 572 910 1440 1544 1703 2896 3040 572 Size: 338 530 104 159 1193 144 3588 Fragments arranged by size: 3588 1193 530 338 159 144 104 Bpu10I CC'TnA_GC Cuts at: 1940 1940 Size: 6056 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 208 291 3232 4111 4603 4901 208 Size: 83 2941 879 492 298 1363 Fragments arranged by size: 2941 1363 879 492 298 83 BsaI GGTCTCn'nnnn_ Cuts at: 2942 5760 2942 Size: 2818 3238 BsaAI yAC'GTr Cuts at: 3409 3409 Size: 6056 BsaBI GATnn'nnATC Cuts at: 6 1721 1727 2030 3570 5607 6 Size: 1715 6 303 1540 2037 455 Fragments arranged by size: 2037 1715 1540 455 303 6 BsaHI Gr'CG_yC Cuts at: 258 3487 3735 4437 5933 258 Size: 3229 248 702 1496 381 Fragments arranged by size: 3229 1496 702 381 248 BsaWI w'CCGG_w Cuts at: 3245 3766 4444 4718 4865 5276 3245 Size: 521 678 274 147 411 4025 Fragments arranged by size: 4025 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 551 581 2793 2823 551 Size: 30 2212 30 3784 Fragments arranged by size: 3784 2212 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1015 1700 2938 2941 1015 Size: 685 1238 3 4130 Fragments arranged by size: 4130 1238 685 3 BseYI C'CCAG_C Cuts at: 548 3481 4239 4816 5312 548 Size: 2933 758 577 496 1292 Fragments arranged by size: 2933 1292 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1504 2042 1504 Size: 538 5518 BsiEI CG_ry'CG Cuts at: 104 807 950 2389 3598 3644 4852 104 Size: 703 143 1439 1209 46 1208 1308 Fragments arranged by size: 1439 1308 1209 1208 703 143 46 BsiHKAI G_wGCw'C Cuts at: 1941 2260 3070 3848 4038 4830 1941 Size: 319 810 778 190 792 3167 Fragments arranged by size: 3167 810 792 778 319 190 BsmI GAATG_Cn' Cuts at: 606 1495 2064 606 Size: 889 569 4598 Fragments arranged by size: 4598 889 569 BsmBI CGTCTCn'nnnn_ Cuts at: 2769 2769 Size: 6056 Bsp1286I G_dGCh'C Cuts at: 1574 1941 2260 2604 3070 3681 3774 3848 4038 Size: 367 319 344 466 611 93 74 190 Cuts at: 4038 4100 4830 1574 Size: 62 730 2800 Fragments arranged by size: 2800 730 611 466 367 344 319 190 93 74 62 BspEI T'CCGG_A Cuts at: 5276 5276 Size: 6056 BspHI T'CATG_A Cuts at: 1629 1719 2190 5232 1629 Size: 90 471 3042 2453 Fragments arranged by size: 3042 2453 471 90 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1941 2063 3106 3320 3622 4003 1941 Size: 122 1043 214 302 381 3994 Fragments arranged by size: 3994 1043 381 302 214 122 BsrBI CCG'CTC Cuts at: 50 1424 4348 4402 5886 6051 50 Size: 1374 2924 54 1484 165 55 Fragments arranged by size: 2924 1484 1374 165 55 54 BsrDI GCAATG_nn' Cuts at: 968 3968 968 Size: 3000 3056 BsrFI r'CCGG_y Cuts at: 121 4054 4235 4444 121 Size: 3933 181 209 1733 Fragments arranged by size: 3933 1733 209 181 BssSI C'ACGA_G Cuts at: 194 4327 4685 194 Size: 4133 358 1565 Fragments arranged by size: 4133 1565 358 BstAPI GCAn_nnn'nTGC Cuts at: 3433 3433 Size: 6056 BstBI TT'CG_AA Cuts at: 4417 4417 Size: 6056 BstXI CCAn_nnnn'nTGG Cuts at: 2874 3018 2874 Size: 144 5912 BstYI r'GATC_y Cuts at: 1 2103 2185 2464 3081 3548 3571 3906 4152 Size: 2102 82 279 617 467 23 335 246 Cuts at: 4152 5164 5250 5262 5608 1 Size: 1012 86 12 346 449 Fragments arranged by size: 2102 1012 617 467 449 346 335 279 246 86 82 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 6056 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 4006 4187 4350 4006 Size: 181 163 5712 Fragments arranged by size: 5712 181 163 BtsI GCAGTG_nn' Cuts at: 1165 1492 1644 3299 1165 Size: 327 152 1655 3922 Fragments arranged by size: 3922 1655 327 152 ClaI AT'CG_AT Cuts at: 2385 2385 Size: 6056 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 348 383 348 Size: 35 6021 DraI TTT'AAA Cuts at: 3055 3055 Size: 6056 DrdI GACnn_nn'nnGTC Cuts at: 3762 4620 3762 Size: 858 5198 EaeI y'GGCC_r Cuts at: 808 2051 2455 3641 3815 4206 4233 5578 808 Size: 1243 404 1186 174 391 27 1345 1286 Fragments arranged by size: 1345 1286 1243 1186 404 391 174 27 EagI C'GGCC_G Cuts at: 3641 3641 Size: 6056 EarI CTCTTCn'nnn_ Cuts at: 561 2772 2834 2930 2966 4079 4289 561 Size: 2211 62 96 36 1113 210 2328 Fragments arranged by size: 2328 2211 1113 210 96 62 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 4264 4574 4720 5866 4264 Size: 310 146 1146 4454 Fragments arranged by size: 4454 1146 310 146 EcoICRI GAG'CTC Cuts at: 3068 3068 Size: 6056 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 293 572 910 1440 1544 1703 2858 2896 3040 Size: 279 338 530 104 159 1155 38 144 Cuts at: 3040 3881 4313 5060 5328 293 Size: 841 432 747 268 1021 Fragments arranged by size: 1155 1021 841 747 530 432 338 279 268 159 144 104 38 EcoNI CCTnn'n_nnAGG Cuts at: 1583 3019 1583 Size: 1436 4620 EcoO109I rG'GnC_Cy Cuts at: 1588 3195 1588 Size: 1607 4449 EcoRI G'AATT_C Cuts at: 3060 3060 Size: 6056 EcoRV GAT'ATC Cuts at: 2481 2657 2481 Size: 176 5880 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 442 3031 4075 442 Size: 2589 1044 2423 Fragments arranged by size: 2589 2423 1044 FspI TGC'GCA Cuts at: 3837 3837 Size: 6056 HaeII r_GCGC'y Cuts at: 3490 3738 4760 5739 3490 Size: 248 1022 979 3807 Fragments arranged by size: 3807 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 163 195 1923 1955 3898 3928 3930 3960 163 Size: 32 1728 32 1943 30 2 30 2259 Fragments arranged by size: 2259 1943 1728 32 32 30 30 2 HincII GTy'rAC Cuts at: 840 3095 840 Size: 2255 3801 KasI G'GCGC_C Cuts at: 3486 3734 3486 Size: 248 5808 KpnI G_GTAC'C Cuts at: 2171 3076 2171 Size: 905 5151 MfeI C'AATT_G Cuts at: 407 407 Size: 6056 MluI A'CGCG_T Cuts at: 5489 5489 Size: 6056 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 970 4727 4911 970 Size: 3757 184 2115 Fragments arranged by size: 3757 2115 184 MscI TGG'CCA Cuts at: 2053 2457 3817 2053 Size: 404 1360 4292 Fragments arranged by size: 4292 1360 404 MslI CAynn'nnrTG Cuts at: 395 509 755 1247 1883 2036 2047 4045 4172 Size: 114 246 492 636 153 11 1998 127 Cuts at: 4172 395 Size: 2279 Fragments arranged by size: 2279 1998 636 492 246 153 127 114 11 MspA1I CmG'CkG Cuts at: 640 2836 2945 3167 3481 3841 4854 5099 640 Size: 2196 109 222 314 360 1013 245 1597 Fragments arranged by size: 2196 1597 1013 360 314 245 222 109 NaeI GCC'GGC Cuts at: 123 4237 123 Size: 4114 1942 NarI GG'CG_CC Cuts at: 3487 3735 3487 Size: 248 5808 NcoI C'CATG_G Cuts at: 107 107 Size: 6056 NdeI CA'TA_TG Cuts at: 900 1274 900 Size: 374 5682 NgoMIV G'CCGG_C Cuts at: 121 4235 121 Size: 4114 1942 NspI r_CATG'y Cuts at: 1391 1937 3109 3609 4516 1391 Size: 546 1172 500 907 2931 Fragments arranged by size: 2931 1172 907 546 500 PciI A'CATG_T Cuts at: 1933 4512 1933 Size: 2579 3477 PmeI GTTT'AAAC Cuts at: 3055 3055 Size: 6056 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5195 5227 5195 Size: 32 6024 PpuMI rG'GwC_Cy Cuts at: 1588 1588 Size: 6056 PspXI vC'TCGA_Gb Cuts at: 329 329 Size: 6056 PstI C_TGCA'G Cuts at: 744 1172 3103 3788 744 Size: 428 1931 685 3012 Fragments arranged by size: 3012 1931 685 428 PvuI CG_AT'CG Cuts at: 104 2389 104 Size: 2285 3771 PvuII CAG'CTG Cuts at: 640 2836 3481 3841 640 Size: 2196 645 360 2855 Fragments arranged by size: 2855 2196 645 360 RsrII CG'GwC_CG Cuts at: 4251 4251 Size: 6056 SacI G_AGCT'C Cuts at: 3070 3070 Size: 6056 SalI G'TCGA_C Cuts at: 3093 3093 Size: 6056 SapI GCTCTTCn'nnn_ Cuts at: 2966 4079 4289 2966 Size: 1113 210 4733 Fragments arranged by size: 4733 1113 210 SbfI CC_TGCA'GG Cuts at: 3103 3103 Size: 6056 ScaI AGT'ACT Cuts at: 156 986 5573 156 Size: 830 4587 639 Fragments arranged by size: 4587 830 639 SfcI C'TryA_G Cuts at: 33 740 921 1168 1565 1778 2180 2732 3099 Size: 707 181 247 397 213 402 552 367 Cuts at: 3099 3784 4777 4968 33 Size: 685 993 191 1121 Fragments arranged by size: 1121 993 707 685 552 402 397 367 247 213 191 181 SfoI GGC'GCC Cuts at: 3488 3736 3488 Size: 248 5808 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6056 SmaI CCC'GGG Cuts at: 3078 3078 Size: 6056 SmlI C'TyrA_G Cuts at: 187 306 329 3211 4126 4618 4880 187 Size: 119 23 2882 915 492 262 1363 Fragments arranged by size: 2882 1363 915 492 262 119 23 SpeI A'CTAG_T Cuts at: 67 67 Size: 6056 SphI G_CATG'C Cuts at: 3109 3609 3109 Size: 500 5556 SspI AAT'ATT Cuts at: 2342 2342 Size: 6056 StuI AGG'CCT Cuts at: 1119 1119 Size: 6056 StyI C'CwwG_G Cuts at: 107 2952 3190 3544 107 Size: 2845 238 354 2619 Fragments arranged by size: 2845 2619 354 238 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 726 1376 3639 3772 4231 4440 726 Size: 650 2263 133 459 209 2342 Fragments arranged by size: 2342 2263 650 459 209 133 TatI w'GTAC_w Cuts at: 154 984 1869 2345 2594 5571 154 Size: 830 885 476 249 2977 639 Fragments arranged by size: 2977 885 830 639 476 249 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 551 583 2793 2825 5573 5605 551 Size: 32 2210 32 2748 32 1002 Fragments arranged by size: 2748 2210 1002 32 32 32 Tth111I GACn'n_nGTC Cuts at: 3853 3853 Size: 6056 XbaI T'CTAG_A Cuts at: 3087 3087 Size: 6056 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3551 3551 Size: 6056 XhoI C'TCGA_G Cuts at: 329 329 Size: 6056 XmaI C'CCGG_G Cuts at: 3076 3076 Size: 6056 XmnI GAAnn'nnTTC Cuts at: 2064 2064 Size: 6056 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I DraIII FseI FspAI HindIII HpaI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI ZraI