(Linear) (Six-base) MAPSORT of: KIEE2674.seq Check: 9791 from: 1 to: 2943 KIEE2674.orf With 182 enzymes: SgfI * February 27, 2017 10:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1833 1955 2943 Size: 1833 122 988 Fragments arranged by size: 1833 988 122 Acc65I G'GTAC_C Cuts at: 0 2059 2943 Size: 2059 884 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 185 2750 2943 Size: 185 2565 193 Fragments arranged by size: 2565 193 185 AflIII A'CryG_T Cuts at: 0 1825 2943 Size: 1825 1118 AleI CACnn'nnGTG Cuts at: 0 1939 2943 Size: 1939 1004 ApaLI G'TGCA_C Cuts at: 0 2148 2943 Size: 2148 795 ApoI r'AATT_y Cuts at: 0 419 558 1343 1369 2943 Size: 419 139 785 26 1574 Fragments arranged by size: 1574 785 419 139 26 AseI AT'TA_AT Cuts at: 0 610 2943 Size: 610 2333 AvaI C'yCGr_G Cuts at: 0 221 2217 2943 Size: 221 1996 726 Fragments arranged by size: 1996 726 221 BanI G'GyrC_C Cuts at: 0 2059 2943 Size: 2059 884 BanII G_rGCy'C Cuts at: 0 1466 2943 Size: 1466 1477 BbsI GAAGACnn'nnnn_ Cuts at: 0 2810 2943 Size: 2810 133 BciVI GTATCCnnnnn_n' Cuts at: 0 2462 2625 2943 Size: 2462 163 318 Fragments arranged by size: 2462 318 163 BclI T'GATC_A Cuts at: 0 405 1647 2943 Size: 405 1242 1296 Fragments arranged by size: 1296 1242 405 BglII A'GATC_T Cuts at: 0 1995 2943 Size: 1995 948 Bme1580I G_kGCm'C Cuts at: 0 2152 2496 2943 Size: 2152 344 447 Fragments arranged by size: 2152 447 344 BmgBI CAC'GTC Cuts at: 0 1026 2943 Size: 1026 1917 BmrI ACTGGGnnnn_n' Cuts at: 0 2250 2471 2943 Size: 2250 221 472 Fragments arranged by size: 2250 472 221 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 464 802 1332 1436 1595 2788 2932 2943 Size: 464 338 530 104 159 1193 144 11 Fragments arranged by size: 1193 530 464 338 159 144 104 11 Bpu10I CC'TnA_GC Cuts at: 0 1832 2943 Size: 1832 1111 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 183 2943 Size: 100 83 2760 Fragments arranged by size: 2760 100 83 BsaI GGTCTCn'nnnn_ Cuts at: 0 2834 2943 Size: 2834 109 BsaBI GATnn'nnATC Cuts at: 0 1613 1619 1922 2943 Size: 1613 6 303 1021 Fragments arranged by size: 1613 1021 303 6 BsaHI Gr'CG_yC Cuts at: 0 150 2943 Size: 150 2793 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 443 473 2685 2715 2943 Size: 443 30 2212 30 228 Fragments arranged by size: 2212 443 228 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 907 1592 2830 2833 2943 Size: 907 685 1238 3 110 Fragments arranged by size: 1238 907 685 110 3 BseYI C'CCAG_C Cuts at: 0 440 2943 Size: 440 2503 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1396 1934 2943 Size: 1396 538 1009 Fragments arranged by size: 1396 1009 538 BsiEI CG_ry'CG Cuts at: 0 699 842 2281 2943 Size: 699 143 1439 662 Fragments arranged by size: 1439 699 662 143 BsiHKAI G_wGCw'C Cuts at: 0 1833 2152 2943 Size: 1833 319 791 Fragments arranged by size: 1833 791 319 BsmI GAATG_Cn' Cuts at: 0 498 1387 1956 2943 Size: 498 889 569 987 Fragments arranged by size: 987 889 569 498 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2661 2943 Size: 2661 282 Bsp1286I G_dGCh'C Cuts at: 0 1466 1833 2152 2496 2943 Size: 1466 367 319 344 447 Fragments arranged by size: 1466 447 367 344 319 BspHI T'CATG_A Cuts at: 0 1521 1611 2082 2943 Size: 1521 90 471 861 Fragments arranged by size: 1521 861 471 90 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1833 1955 2943 Size: 1833 122 988 Fragments arranged by size: 1833 988 122 BsrBI CCG'CTC Cuts at: 0 1316 2943 Size: 1316 1627 BsrDI GCAATG_nn' Cuts at: 0 860 2943 Size: 860 2083 BsrFI r'CCGG_y Cuts at: 0 13 2943 Size: 13 2930 BssSI C'ACGA_G Cuts at: 0 86 2943 Size: 86 2857 BstXI CCAn_nnnn'nTGG Cuts at: 0 2766 2910 2943 Size: 2766 144 33 Fragments arranged by size: 2766 144 33 BstYI r'GATC_y Cuts at: 0 1995 2077 2356 2943 Size: 1995 82 279 587 Fragments arranged by size: 1995 587 279 82 BtsI GCAGTG_nn' Cuts at: 0 1057 1384 1536 2943 Size: 1057 327 152 1407 Fragments arranged by size: 1407 1057 327 152 ClaI AT'CG_AT Cuts at: 0 2277 2943 Size: 2277 666 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 240 275 2943 Size: 240 35 2668 Fragments arranged by size: 2668 240 35 EaeI y'GGCC_r Cuts at: 0 700 1943 2347 2943 Size: 700 1243 404 596 Fragments arranged by size: 1243 700 596 404 EarI CTCTTCn'nnn_ Cuts at: 0 453 2664 2726 2822 2858 2943 Size: 453 2211 62 96 36 85 Fragments arranged by size: 2211 453 96 85 62 36 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 185 464 802 1332 1436 1595 2750 2788 Size: 185 279 338 530 104 159 1155 38 Cuts at: 2788 2932 2943 Size: 144 11 Fragments arranged by size: 1155 530 338 279 185 159 144 104 38 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 1475 2911 2943 Size: 1475 1436 32 Fragments arranged by size: 1475 1436 32 EcoO109I rG'GnC_Cy Cuts at: 0 1480 2943 Size: 1480 1463 EcoRV GAT'ATC Cuts at: 0 2373 2549 2943 Size: 2373 176 394 Fragments arranged by size: 2373 394 176 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 334 2923 2943 Size: 334 2589 20 Fragments arranged by size: 2589 334 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 55 87 1815 1847 2943 Size: 55 32 1728 32 1096 Fragments arranged by size: 1728 1096 55 32 32 HincII GTy'rAC Cuts at: 0 732 2943 Size: 732 2211 KpnI G_GTAC'C Cuts at: 0 2063 2943 Size: 2063 880 MfeI C'AATT_G Cuts at: 0 299 2943 Size: 299 2644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 862 2943 Size: 862 2081 MscI TGG'CCA Cuts at: 0 1945 2349 2943 Size: 1945 404 594 Fragments arranged by size: 1945 594 404 MslI CAynn'nnrTG Cuts at: 0 287 401 647 1139 1775 1928 1939 2943 Size: 287 114 246 492 636 153 11 1004 Fragments arranged by size: 1004 636 492 287 246 153 114 11 MspA1I CmG'CkG Cuts at: 0 532 2728 2837 2943 Size: 532 2196 109 106 Fragments arranged by size: 2196 532 109 106 NaeI GCC'GGC Cuts at: 0 15 2943 Size: 15 2928 NdeI CA'TA_TG Cuts at: 0 792 1166 2943 Size: 792 374 1777 Fragments arranged by size: 1777 792 374 NgoMIV G'CCGG_C Cuts at: 0 13 2943 Size: 13 2930 NspI r_CATG'y Cuts at: 0 1283 1829 2943 Size: 1283 546 1114 Fragments arranged by size: 1283 1114 546 PciI A'CATG_T Cuts at: 0 1825 2943 Size: 1825 1118 PpuMI rG'GwC_Cy Cuts at: 0 1480 2943 Size: 1480 1463 PspXI vC'TCGA_Gb Cuts at: 0 221 2943 Size: 221 2722 PstI C_TGCA'G Cuts at: 0 636 1064 2943 Size: 636 428 1879 Fragments arranged by size: 1879 636 428 PvuI CG_AT'CG Cuts at: 0 2281 2943 Size: 2281 662 PvuII CAG'CTG Cuts at: 0 532 2728 2943 Size: 532 2196 215 Fragments arranged by size: 2196 532 215 SapI GCTCTTCn'nnn_ Cuts at: 0 2858 2943 Size: 2858 85 ScaI AGT'ACT Cuts at: 0 48 878 2943 Size: 48 830 2065 Fragments arranged by size: 2065 830 48 SfcI C'TryA_G Cuts at: 0 632 813 1060 1457 1670 2072 2624 2943 Size: 632 181 247 397 213 402 552 319 Fragments arranged by size: 632 552 402 397 319 247 213 181 SmlI C'TyrA_G Cuts at: 0 79 198 221 2943 Size: 79 119 23 2722 Fragments arranged by size: 2722 119 79 23 SspI AAT'ATT Cuts at: 0 2234 2943 Size: 2234 709 StuI AGG'CCT Cuts at: 0 1011 2943 Size: 1011 1932 StyI C'CwwG_G Cuts at: 0 2844 2943 Size: 2844 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 618 1268 2943 Size: 618 650 1675 Fragments arranged by size: 1675 650 618 TatI w'GTAC_w Cuts at: 0 46 876 1761 2237 2486 2943 Size: 46 830 885 476 249 457 Fragments arranged by size: 885 830 476 457 249 46 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 443 475 2685 2717 2943 Size: 443 32 2210 32 226 Fragments arranged by size: 2210 443 226 32 32 XhoI C'TCGA_G Cuts at: 0 221 2943 Size: 221 2722 XmnI GAAnn'nnTTC Cuts at: 0 1956 2943 Size: 1956 987 Enzymes that do cut: AarI Acc65I AcuI AflIII AleI ApaLI ApoI AseI AvaI BanI BanII BbsI BciVI BclI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstXI BstYI BtsI ClaI CspCI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII KpnI MfeI MmeI MscI MslI MspA1I NaeI NdeI NgoMIV NspI PciI PpuMI PspXI PstI PvuI PvuII SapI ScaI SfcI SmlI SspI StuI StyI TaqII TatI TstI XhoI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BfrBI BglI BlpI BmtI BplI BsaAI BsaWI BsiWI BspEI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI DraI DraIII DrdI EagI EciI EcoICRI EcoRI FseI FspI FspAI HaeII HindIII HpaI KasI MluI NarI NcoI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI RsrII SacI SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XcmI XmaI ZraI