(Linear) (Six-base) MAPSORT of: KIEE2648.seq Check: 8630 from: 1 to: 2400 KIEE2648.orf With 182 enzymes: SgfI * November 11, 2016 16:52 .. AccI GT'mk_AC Cuts at: 0 469 2270 2400 Size: 469 1801 130 Fragments arranged by size: 1801 469 130 Acc65I G'GTAC_C Cuts at: 0 792 2400 Size: 792 1608 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1403 1594 1609 2325 2400 Size: 1403 191 15 716 75 Fragments arranged by size: 1403 716 191 75 15 AflIII A'CryG_T Cuts at: 0 315 2400 Size: 315 2085 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 658 1585 2400 Size: 658 927 815 Fragments arranged by size: 927 815 658 AlwNI CAG_nnn'CTG Cuts at: 0 75 585 1177 1836 2400 Size: 75 510 592 659 564 Fragments arranged by size: 659 592 564 510 75 ApoI r'AATT_y Cuts at: 0 198 1159 1201 1322 1841 1906 2335 2400 Size: 198 961 42 121 519 65 429 65 Fragments arranged by size: 961 519 429 198 121 65 65 42 AseI AT'TA_AT Cuts at: 0 2170 2224 2400 Size: 2170 54 176 Fragments arranged by size: 2170 176 54 AvaI C'yCGr_G Cuts at: 0 33 2400 Size: 33 2367 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 275 308 2400 Size: 275 33 2092 Fragments arranged by size: 2092 275 33 BamHI G'GATC_C Cuts at: 0 2023 2400 Size: 2023 377 BanI G'GyrC_C Cuts at: 0 792 2400 Size: 792 1608 BanII G_rGCy'C Cuts at: 0 935 1136 2400 Size: 935 201 1264 Fragments arranged by size: 1264 935 201 BbsI GAAGACnn'nnnn_ Cuts at: 0 233 2400 Size: 233 2167 BbvCI CC'TCA_GC Cuts at: 0 2064 2400 Size: 2064 336 BciVI GTATCCnnnnn_n' Cuts at: 0 842 860 866 2400 Size: 842 18 6 1534 Fragments arranged by size: 1534 842 18 6 BfrBI ATG'CAT Cuts at: 0 1406 2400 Size: 1406 994 BglII A'GATC_T Cuts at: 0 1292 2400 Size: 1292 1108 BmgBI CAC'GTC Cuts at: 0 1195 2400 Size: 1195 1205 BmrI ACTGGGnnnn_n' Cuts at: 0 85 1287 1501 1770 2400 Size: 85 1202 214 269 630 Fragments arranged by size: 1202 630 269 214 85 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 512 842 2267 2400 Size: 512 330 1425 133 Fragments arranged by size: 1425 512 330 133 Bpu10I CC'TnA_GC Cuts at: 0 2064 2400 Size: 2064 336 BsaI GGTCTCn'nnnn_ Cuts at: 0 1054 2400 Size: 1054 1346 BsaAI yAC'GTr Cuts at: 0 1109 2400 Size: 1109 1291 BsaBI GATnn'nnATC Cuts at: 0 1291 2400 Size: 1291 1109 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1123 1153 1918 1948 2400 Size: 1123 30 765 30 452 Fragments arranged by size: 1123 765 452 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1144 2400 Size: 1144 1256 BseYI C'CCAG_C Cuts at: 0 1536 2400 Size: 1536 864 BsiEI CG_ry'CG Cuts at: 0 8 2400 Size: 8 2392 BsiHKAI G_wGCw'C Cuts at: 0 935 1339 2400 Size: 935 404 1061 Fragments arranged by size: 1061 935 404 BsmI GAATG_Cn' Cuts at: 0 720 2400 Size: 720 1680 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1142 2400 Size: 1142 1258 Bsp1286I G_dGCh'C Cuts at: 0 935 1136 1339 2400 Size: 935 201 203 1061 Fragments arranged by size: 1061 935 203 201 BspHI T'CATG_A Cuts at: 0 1421 2400 Size: 1421 979 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 856 2256 2400 Size: 856 1400 144 Fragments arranged by size: 1400 856 144 BsrDI GCAATG_nn' Cuts at: 0 640 2400 Size: 640 1760 BsrFI r'CCGG_y Cuts at: 0 60 2400 Size: 60 2340 BsrGI T'GTAC_A Cuts at: 0 1367 1435 2400 Size: 1367 68 965 Fragments arranged by size: 1367 965 68 BstEII G'GTnAC_C Cuts at: 0 743 2400 Size: 743 1657 BstXI CCAn_nnnn'nTGG Cuts at: 0 1008 2400 Size: 1008 1392 BstYI r'GATC_y Cuts at: 0 1292 2023 2400 Size: 1292 731 377 Fragments arranged by size: 1292 731 377 BstZ17I GTA'TAC Cuts at: 0 470 2400 Size: 470 1930 Bsu36I CC'TnA_GG Cuts at: 0 860 2400 Size: 860 1540 BtgI C'CryG_G Cuts at: 0 800 2027 2400 Size: 800 1227 373 Fragments arranged by size: 1227 800 373 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 714 2400 Size: 714 1686 DraI TTT'AAA Cuts at: 0 419 954 2016 2400 Size: 419 535 1062 384 Fragments arranged by size: 1062 535 419 384 EaeI y'GGCC_r Cuts at: 0 5 2400 Size: 5 2395 EagI C'GGCC_G Cuts at: 0 5 2400 Size: 5 2395 EarI CTCTTCn'nnn_ Cuts at: 0 701 1703 2296 2400 Size: 701 1002 593 104 Fragments arranged by size: 1002 701 593 104 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 79 2400 Size: 79 2321 EcoICRI GAG'CTC Cuts at: 0 933 2400 Size: 933 1467 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 512 842 1403 1594 1609 2267 2325 2400 Size: 512 330 561 191 15 658 58 75 Fragments arranged by size: 658 561 512 330 191 75 58 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 863 2400 Size: 863 1537 EcoO109I rG'GnC_Cy Cuts at: 0 1503 2400 Size: 1503 897 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1084 1116 2295 2327 2400 Size: 1084 32 1179 32 73 Fragments arranged by size: 1179 1084 73 32 32 HindIII A'AGCT_T Cuts at: 0 2008 2400 Size: 2008 392 KpnI G_GTAC'C Cuts at: 0 796 2400 Size: 796 1604 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2232 2400 Size: 2232 168 MslI CAynn'nnrTG Cuts at: 0 160 1216 1780 2400 Size: 160 1056 564 620 Fragments arranged by size: 1056 620 564 160 MspA1I CmG'CkG Cuts at: 0 585 1365 1734 1900 2400 Size: 585 780 369 166 500 Fragments arranged by size: 780 585 500 369 166 NaeI GCC'GGC Cuts at: 0 62 2400 Size: 62 2338 NcoI C'CATG_G Cuts at: 0 800 2400 Size: 800 1600 NdeI CA'TA_TG Cuts at: 0 438 480 2400 Size: 438 42 1920 Fragments arranged by size: 1920 438 42 NgoMIV G'CCGG_C Cuts at: 0 60 2400 Size: 60 2340 NsiI A_TGCA'T Cuts at: 0 1408 2400 Size: 1408 992 NspI r_CATG'y Cuts at: 0 319 2400 Size: 319 2081 PciI A'CATG_T Cuts at: 0 315 2400 Size: 315 2085 PflMI CCAn_nnn'nTGG Cuts at: 0 75 2400 Size: 75 2325 PpuMI rG'GwC_Cy Cuts at: 0 1503 2400 Size: 1503 897 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1634 1666 2400 Size: 1634 32 734 Fragments arranged by size: 1634 734 32 PvuII CAG'CTG Cuts at: 0 585 1365 1734 1900 2400 Size: 585 780 369 166 500 Fragments arranged by size: 780 585 500 369 166 SacI G_AGCT'C Cuts at: 0 935 2400 Size: 935 1465 SanDI GG'GwC_CC Cuts at: 0 1503 2400 Size: 1503 897 SapI GCTCTTCn'nnn_ Cuts at: 0 1703 2296 2400 Size: 1703 593 104 Fragments arranged by size: 1703 593 104 SfcI C'TryA_G Cuts at: 0 1067 2319 2400 Size: 1067 1252 81 Fragments arranged by size: 1252 1067 81 SmaI CCC'GGG Cuts at: 0 35 2400 Size: 35 2365 SspI AAT'ATT Cuts at: 0 1546 2400 Size: 1546 854 StyI C'CwwG_G Cuts at: 0 573 800 1498 2085 2400 Size: 573 227 698 587 315 Fragments arranged by size: 698 587 573 315 227 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1697 2400 Size: 1697 703 TatI w'GTAC_w Cuts at: 0 1367 1435 2400 Size: 1367 68 965 Fragments arranged by size: 1367 965 68 Tth111I GACn'n_nGTC Cuts at: 0 544 2400 Size: 544 1856 XbaI T'CTAG_A Cuts at: 0 1144 2400 Size: 1144 1256 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 101 219 269 357 1674 2400 Size: 101 118 50 88 1317 726 Fragments arranged by size: 1317 726 118 101 88 50 XmaI C'CCGG_G Cuts at: 0 33 2400 Size: 33 2367 Enzymes that do cut: AccI Acc65I AcuI AflIII AlfI AlwNI ApoI AseI AvaI BaeI BamHI BanI BanII BbsI BbvCI BciVI BfrBI BglII BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI DraI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HindIII KpnI MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PciI PflMI PpuMI PsrI PvuII SacI SanDI SapI SfcI SmaI SspI StyI TaqII TatI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AclI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI AvrII BbeI BcgI BclI BglI BlpI Bme1580I BmtI BplI BpuEI BsaHI BsaWI BsgI BsiWI BspEI BsrBI BssHII BssSI BstAPI BstBI BtsI ClaI CspCI DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HpaI KasI MfeI MluI MscI NarI NheI NotI NruI PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI StuI SwaI TstI XhoI XmnI ZraI