(Linear) (Six-base) MAPSORT of: KIEE3374.seq Check: 8272 from: 1 to: 3351 KIEE3374.orf With 182 enzymes: SgfI * October 24, 2019 20:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 503 2274 2349 3351 Size: 503 1771 75 1002 Fragments arranged by size: 1771 1002 503 75 AatII G_ACGT'C Cuts at: 0 2269 3351 Size: 2269 1082 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 952 1693 1902 3351 Size: 952 741 209 1449 Fragments arranged by size: 1449 952 741 209 AflIII A'CryG_T Cuts at: 0 878 3351 Size: 878 2473 AgeI A'CCGG_T Cuts at: 0 1886 3351 Size: 1886 1465 AhdI GACnn_n'nnGTC Cuts at: 0 2315 3351 Size: 2315 1036 AleI CACnn'nnGTG Cuts at: 0 646 2428 2593 3351 Size: 646 1782 165 758 Fragments arranged by size: 1782 758 646 165 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 652 2425 2936 3061 3351 Size: 652 1773 511 125 290 Fragments arranged by size: 1773 652 511 290 125 AlwNI CAG_nnn'CTG Cuts at: 0 46 309 453 759 1858 2124 2165 2697 Size: 46 263 144 306 1099 266 41 532 Cuts at: 2697 2991 3351 Size: 294 360 Fragments arranged by size: 1099 532 360 306 294 266 263 144 46 41 ApaI G_GGCC'C Cuts at: 0 201 303 789 1565 3351 Size: 201 102 486 776 1786 Fragments arranged by size: 1786 776 486 201 102 ApaLI G'TGCA_C Cuts at: 0 314 976 1594 3351 Size: 314 662 618 1757 Fragments arranged by size: 1757 662 618 314 ApoI r'AATT_y Cuts at: 0 1011 1984 3351 Size: 1011 973 1367 Fragments arranged by size: 1367 1011 973 AvaI C'yCGr_G Cuts at: 0 437 1846 1964 2242 3351 Size: 437 1409 118 278 1109 Fragments arranged by size: 1409 1109 437 278 118 BamHI G'GATC_C Cuts at: 0 1298 1607 1643 3351 Size: 1298 309 36 1708 Fragments arranged by size: 1708 1298 309 36 BanI G'GyrC_C Cuts at: 0 36 146 409 442 554 616 628 668 Size: 36 110 263 33 112 62 12 40 Cuts at: 668 1079 1876 2173 2484 3351 Size: 411 797 297 311 867 Fragments arranged by size: 867 797 411 311 297 263 112 110 62 40 36 33 12 BanII G_rGCy'C Cuts at: 0 201 303 789 1565 1841 2219 2375 2624 Size: 201 102 486 776 276 378 156 249 Cuts at: 2624 2660 2763 3351 Size: 36 103 588 Fragments arranged by size: 776 588 486 378 276 249 201 156 103 102 36 BbeI G_GCGC'C Cuts at: 0 150 446 558 672 1880 3351 Size: 150 296 112 114 1208 1471 Fragments arranged by size: 1471 1208 296 150 114 112 BbsI GAAGACnn'nnnn_ Cuts at: 0 1533 1896 3229 3351 Size: 1533 363 1333 122 Fragments arranged by size: 1533 1333 363 122 BbvCI CC'TCA_GC Cuts at: 0 559 3351 Size: 559 2792 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 935 969 2385 2419 3351 Size: 935 34 1416 34 932 Fragments arranged by size: 1416 935 932 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 542 599 1109 2223 3351 Size: 542 57 510 1114 1128 Fragments arranged by size: 1128 1114 542 510 57 BlpI GC'TnA_GC Cuts at: 0 1835 3351 Size: 1835 1516 Bme1580I G_kGCm'C Cuts at: 0 201 236 303 318 789 980 1565 1598 Size: 201 35 67 15 471 191 585 33 Cuts at: 1598 2176 2590 2634 3351 Size: 578 414 44 717 Fragments arranged by size: 717 585 578 471 414 201 191 67 44 35 33 15 BmgBI CAC'GTC Cuts at: 0 1941 3351 Size: 1941 1410 BmrI ACTGGGnnnn_n' Cuts at: 0 1095 1749 2127 3351 Size: 1095 654 378 1224 Fragments arranged by size: 1224 1095 654 378 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1272 3351 Size: 1272 2079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 68 138 151 207 732 736 852 1021 Size: 68 70 13 56 525 4 116 169 Cuts at: 1021 1032 1425 1597 1969 2632 2690 2968 3316 Size: 11 393 172 372 663 58 278 348 Cuts at: 3316 3351 Size: 35 Fragments arranged by size: 663 525 393 372 348 278 172 169 116 70 68 58 56 35 13 11 4 Bpu10I CC'TnA_GC Cuts at: 0 559 1549 2822 3269 3351 Size: 559 990 1273 447 82 Fragments arranged by size: 1273 990 559 447 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2548 2918 3351 Size: 2548 370 433 Fragments arranged by size: 2548 433 370 BsaI GGTCTCn'nnnn_ Cuts at: 0 865 1401 3136 3351 Size: 865 536 1735 215 Fragments arranged by size: 1735 865 536 215 BsaAI yAC'GTr Cuts at: 0 1788 3351 Size: 1788 1563 BsaBI GATnn'nnATC Cuts at: 0 2809 3351 Size: 2809 542 BsaHI Gr'CG_yC Cuts at: 0 102 147 443 555 669 1101 1877 1934 Size: 102 45 296 112 114 432 776 57 Cuts at: 1934 2266 2723 3351 Size: 332 457 628 Fragments arranged by size: 776 628 457 432 332 296 114 112 102 57 45 BsaWI w'CCGG_w Cuts at: 0 815 1886 3351 Size: 815 1071 1465 Fragments arranged by size: 1465 1071 815 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 117 120 367 370 477 1261 1408 1807 Size: 117 3 247 3 107 784 147 399 Cuts at: 1807 3351 Size: 1544 Fragments arranged by size: 1544 784 399 247 147 117 107 3 3 BseYI C'CCAG_C Cuts at: 0 67 115 161 302 446 1260 1820 2219 Size: 67 48 46 141 144 814 560 399 Cuts at: 2219 2583 2829 3001 3351 Size: 364 246 172 350 Fragments arranged by size: 814 560 399 364 350 246 172 144 141 67 48 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 813 2377 2449 2452 2527 2909 2977 Size: 523 290 1564 72 3 75 382 68 Cuts at: 2977 3031 3351 Size: 54 320 Fragments arranged by size: 1564 523 382 320 290 75 72 68 54 3 BsiEI CG_ry'CG Cuts at: 0 698 1887 2757 3351 Size: 698 1189 870 594 Fragments arranged by size: 1189 870 698 594 BsiHKAI G_wGCw'C Cuts at: 0 275 318 860 980 1258 1598 2204 2333 Size: 275 43 542 120 278 340 606 129 Cuts at: 2333 2624 2771 3351 Size: 291 147 580 Fragments arranged by size: 606 580 542 340 291 278 275 147 129 120 43 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3017 3334 3351 Size: 3017 317 17 Fragments arranged by size: 3017 317 17 Bsp1286I G_dGCh'C Cuts at: 0 201 236 275 303 318 789 860 980 Size: 201 35 39 28 15 471 71 120 Cuts at: 980 1258 1565 1598 1841 2176 2204 2219 2333 Size: 278 307 33 243 335 28 15 114 Cuts at: 2333 2375 2590 2624 2634 2660 2763 2771 3351 Size: 42 215 34 10 26 103 8 580 Fragments arranged by size: 580 471 335 307 278 243 215 201 120 114 103 71 42 39 35 34 33 28 28 26 15 15 10 8 BspEI T'CCGG_A Cuts at: 0 815 3351 Size: 815 2536 BspHI T'CATG_A Cuts at: 0 1442 3218 3351 Size: 1442 1776 133 Fragments arranged by size: 1776 1442 133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 482 503 1610 2274 2349 3098 3351 Size: 482 21 1107 664 75 749 253 Fragments arranged by size: 1107 749 664 482 253 75 21 BsrBI CCG'CTC Cuts at: 0 152 807 1440 2304 3351 Size: 152 655 633 864 1047 Fragments arranged by size: 1047 864 655 633 152 BsrFI r'CCGG_y Cuts at: 0 215 625 1886 2481 3302 3351 Size: 215 410 1261 595 821 49 Fragments arranged by size: 1261 821 595 410 215 49 BssSI C'ACGA_G Cuts at: 0 1188 1957 3351 Size: 1188 769 1394 Fragments arranged by size: 1394 1188 769 BstAPI GCAn_nnn'nTGC Cuts at: 0 1602 2484 3185 3351 Size: 1602 882 701 166 Fragments arranged by size: 1602 882 701 166 BstXI CCAn_nnnn'nTGG Cuts at: 0 75 589 2064 3351 Size: 75 514 1475 1287 Fragments arranged by size: 1475 1287 514 75 BstYI r'GATC_y Cuts at: 0 812 1298 1607 1643 1775 2114 3113 3351 Size: 812 486 309 36 132 339 999 238 Fragments arranged by size: 999 812 486 339 309 238 132 36 Bsu36I CC'TnA_GG Cuts at: 0 576 612 1391 1859 3351 Size: 576 36 779 468 1492 Fragments arranged by size: 1492 779 576 468 36 BtgI C'CryG_G Cuts at: 0 293 503 1277 2426 3351 Size: 293 210 774 1149 925 Fragments arranged by size: 1149 925 774 293 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2388 3351 Size: 2388 963 BtsI GCAGTG_nn' Cuts at: 0 2320 2729 2908 3351 Size: 2320 409 179 443 Fragments arranged by size: 2320 443 409 179 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1725 1760 3351 Size: 1725 35 1591 Fragments arranged by size: 1725 1591 35 DrdI GACnn_nn'nnGTC Cuts at: 0 879 1939 3351 Size: 879 1060 1412 Fragments arranged by size: 1412 1060 879 EaeI y'GGCC_r Cuts at: 0 695 1175 2066 2273 3284 3351 Size: 695 480 891 207 1011 67 Fragments arranged by size: 1011 891 695 480 207 67 EagI C'GGCC_G Cuts at: 0 695 3351 Size: 695 2656 EarI CTCTTCn'nnn_ Cuts at: 0 600 1345 2936 3351 Size: 600 745 1591 415 Fragments arranged by size: 1591 745 600 415 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 761 2306 2463 3351 Size: 761 1545 157 888 Fragments arranged by size: 1545 888 761 157 EcoICRI GAG'CTC Cuts at: 0 2622 3351 Size: 2622 729 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 68 138 151 207 732 736 852 952 Size: 68 70 13 56 525 4 116 100 Cuts at: 952 1021 1032 1425 1597 1693 1902 1969 2632 Size: 69 11 393 172 96 209 67 663 Cuts at: 2632 2690 2968 3316 3351 Size: 58 278 348 35 Fragments arranged by size: 663 525 393 348 278 209 172 116 100 96 70 69 68 67 58 56 35 13 11 4 EcoNI CCTnn'n_nnAGG Cuts at: 0 1365 1552 3351 Size: 1365 187 1799 Fragments arranged by size: 1799 1365 187 EcoO109I rG'GnC_Cy Cuts at: 0 43 197 299 534 785 786 1316 1387 Size: 43 154 102 235 251 1 530 71 Cuts at: 1387 1561 1824 1855 2061 2121 2538 3209 3351 Size: 174 263 31 206 60 417 671 142 Fragments arranged by size: 671 530 417 263 251 235 206 174 154 142 102 71 60 43 31 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1047 1170 2730 3351 Size: 1047 123 1560 621 Fragments arranged by size: 1560 1047 621 123 FspI TGC'GCA Cuts at: 0 957 2413 2500 3351 Size: 957 1456 87 851 Fragments arranged by size: 1456 957 851 87 HaeII r_GCGC'y Cuts at: 0 150 161 222 398 446 558 672 719 Size: 150 11 61 176 48 112 114 47 Cuts at: 719 1576 1880 2456 3351 Size: 857 304 576 895 Fragments arranged by size: 895 857 576 304 176 150 114 112 61 48 47 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1400 1432 1753 1785 2301 2333 3351 Size: 1400 32 321 32 516 32 1018 Fragments arranged by size: 1400 1018 516 321 32 32 32 HincII GTy'rAC Cuts at: 0 329 3351 Size: 329 3022 HindIII A'AGCT_T Cuts at: 0 2380 3351 Size: 2380 971 KasI G'GCGC_C Cuts at: 0 146 442 554 668 1876 3351 Size: 146 296 112 114 1208 1475 Fragments arranged by size: 1475 1208 296 146 114 112 MscI TGG'CCA Cuts at: 0 1177 2068 3351 Size: 1177 891 1283 Fragments arranged by size: 1283 1177 891 MslI CAynn'nnrTG Cuts at: 0 646 2080 2353 2428 2506 2531 2593 3351 Size: 646 1434 273 75 78 25 62 758 Fragments arranged by size: 1434 758 646 273 78 75 62 25 MspA1I CmG'CkG Cuts at: 0 63 115 144 453 505 567 999 1019 Size: 63 52 29 309 52 62 432 20 Cuts at: 1019 1184 1403 1812 1820 2223 2583 3163 3255 Size: 165 219 409 8 403 360 580 92 Cuts at: 3255 3327 3351 Size: 72 24 Fragments arranged by size: 580 432 409 403 360 309 219 165 92 72 63 62 52 52 29 24 20 8 NaeI GCC'GGC Cuts at: 0 217 627 3351 Size: 217 410 2724 Fragments arranged by size: 2724 410 217 NarI GG'CG_CC Cuts at: 0 147 443 555 669 1877 3351 Size: 147 296 112 114 1208 1474 Fragments arranged by size: 1474 1208 296 147 114 112 NcoI C'CATG_G Cuts at: 0 2426 3351 Size: 2426 925 NdeI CA'TA_TG Cuts at: 0 849 3351 Size: 849 2502 NgoMIV G'CCGG_C Cuts at: 0 215 625 3351 Size: 215 410 2726 Fragments arranged by size: 2726 410 215 NruI TCG'CGA Cuts at: 0 727 2321 3351 Size: 727 1594 1030 Fragments arranged by size: 1594 1030 727 NspI r_CATG'y Cuts at: 0 2526 2690 3351 Size: 2526 164 661 Fragments arranged by size: 2526 661 164 PasI CC'CwG_GG Cuts at: 0 201 595 781 1704 2183 2194 3168 3351 Size: 201 394 186 923 479 11 974 183 Fragments arranged by size: 974 923 479 394 201 186 183 11 PflMI CCAn_nnn'nTGG Cuts at: 0 46 309 759 2183 3351 Size: 46 263 450 1424 1168 Fragments arranged by size: 1424 1168 450 263 46 PfoI T'CCnGG_A Cuts at: 0 365 1048 2117 2717 2813 2984 3351 Size: 365 683 1069 600 96 171 367 Fragments arranged by size: 1069 683 600 367 365 171 96 PmlI CAC'GTG Cuts at: 0 1788 3351 Size: 1788 1563 PpuMI rG'GwC_Cy Cuts at: 0 1824 2061 2121 3351 Size: 1824 237 60 1230 Fragments arranged by size: 1824 1230 237 60 PspOMI G'GGCC_C Cuts at: 0 197 299 785 1561 3351 Size: 197 102 486 776 1790 Fragments arranged by size: 1790 776 486 197 102 PspXI vC'TCGA_Gb Cuts at: 0 2242 3351 Size: 2242 1109 PstI C_TGCA'G Cuts at: 0 1238 2285 3351 Size: 1238 1047 1066 Fragments arranged by size: 1238 1066 1047 PvuII CAG'CTG Cuts at: 0 999 1184 1812 3351 Size: 999 185 628 1539 Fragments arranged by size: 1539 999 628 185 SacI G_AGCT'C Cuts at: 0 2624 3351 Size: 2624 727 SacII CC_GC'GG Cuts at: 0 506 3351 Size: 506 2845 SapI GCTCTTCn'nnn_ Cuts at: 0 600 2936 3351 Size: 600 2336 415 Fragments arranged by size: 2336 600 415 ScaI AGT'ACT Cuts at: 0 913 3351 Size: 913 2438 SfcI C'TryA_G Cuts at: 0 1234 2281 2745 3351 Size: 1234 1047 464 606 Fragments arranged by size: 1234 1047 606 464 SfiI GGCCn_nnn'nGGCC Cuts at: 0 599 3351 Size: 599 2752 SfoI GGC'GCC Cuts at: 0 148 444 556 670 1878 3351 Size: 148 296 112 114 1208 1473 Fragments arranged by size: 1473 1208 296 148 114 112 SgrAI Cr'CCGG_yG Cuts at: 0 215 3351 Size: 215 3136 SmlI C'TyrA_G Cuts at: 0 1964 2242 2563 2933 3351 Size: 1964 278 321 370 418 Fragments arranged by size: 1964 418 370 321 278 SphI G_CATG'C Cuts at: 0 2526 3351 Size: 2526 825 StuI AGG'CCT Cuts at: 0 124 709 2572 3351 Size: 124 585 1863 779 Fragments arranged by size: 1863 779 585 124 StyI C'CwwG_G Cuts at: 0 1565 2057 2342 2426 3351 Size: 1565 492 285 84 925 Fragments arranged by size: 1565 925 492 285 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 301 1880 2771 2910 3351 Size: 301 1579 891 139 441 Fragments arranged by size: 1579 891 441 301 139 TatI w'GTAC_w Cuts at: 0 820 911 3351 Size: 820 91 2440 Fragments arranged by size: 2440 820 91 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1099 1131 3351 Size: 1099 32 2220 Fragments arranged by size: 2220 1099 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1572 1701 2268 3351 Size: 1572 129 567 1083 Fragments arranged by size: 1572 1083 567 129 XhoI C'TCGA_G Cuts at: 0 1964 2242 3351 Size: 1964 278 1109 Fragments arranged by size: 1964 1109 278 XmnI GAAnn'nnTTC Cuts at: 0 1135 2971 3351 Size: 1135 1836 380 Fragments arranged by size: 1836 1135 380 ZraI GAC'GTC Cuts at: 0 2267 3351 Size: 2267 1084 Enzymes that do cut: AarI AatII AcuI AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII HindIII KasI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI NspI PasI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacI SacII SapI ScaI SfcI SfiI SfoI SgrAI SmlI SphI StuI StyI TaqII TatI TstI XcmI XhoI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AloI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BglII BmtI BsaXI BsiWI BsmI BsrDI BsrGI BssHII BstBI BstEII BstZ17I ClaI DraI DraIII EcoRI EcoRV FseI FspAI HpaI KpnI MfeI MluI MmeI NheI NotI NsiI PacI PciI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SalI SanDI SbfI SexAI SgfI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XmaI