################################## # NO CUT ENZYMES ( 44 enzymes) # ################################## AatII AclI AcsI AflII Alw44I ApaLI ApoI AscI AseI AsnI BbrPI BciVI BfrI BsiWI Bst1107I BstBI BstZ17I DraI EcoRI EcoRV EcoT22I FseI HpaI MfeI MluI MunI NdeI NruI NsiI NspV PacI PmaCI PmeI PmlI PshBI Psp1406I SbfI SfiI SfuI SnaBI SpeI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AgeI 1307 ACCGGT A^CCGGT AhdI 3105 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 3105 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3518 TGCGCA TGC^GCA AvrII 5160 CCTAGG C^CTAGG BglII 1317 AGATCT A^GATCT BlnI 5160 CCTAGG C^CTAGG BsmBI 5064 CGTCTC CGTCTC(1/5) BspDI 141 ATCGAT AT^CGAT BspHI 647 TCATGA T^CATGA BstEII 6937 GGTTACC G^GT[ACGT]ACC BstPI 6937 GGTTACC G^GT[ACGT]ACC ClaI 141 ATCGAT AT^CGAT CpoI 6116 CGGACCG CG^G[AT]CCG Eam1105I 3105 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 6937 GGTTACC G^GT[ACGT]ACC FspI 3518 TGCGCA TGC^GCA HindIII 2194 AAGCTT A^AGCTT MseI 3397 TTAA T^TAA NheI 6535 GCTAGC G^CTAGC PaeR7I 5303 CTCGAG C^TCGAG PinAI 1307 ACCGGT A^CCGGT PshAI 4770 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 143 CGATCG CGAT^CG RcaI 647 TCATGA T^CATGA RsrII 6116 CGGACCG CG^G[AT]CCG SalI 2533 GTCGAC G^TCGAC SphI 4850 GCATGC GCATG^C SspI 4867 AATATT AAT^ATT Tru9I 3397 TTAA T^TAA XhoI 5303 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AocI 2506 CCTGAGG CC^T[ACGT]AGG 6820 CCTGAGG Aor51HI 6056 AGCGCT AGC^GCT 6374 AGCGCT AspI 241 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 4615 GACTTTGTC BbeI 2845 GGCGCC GGCGC^C 3685 GGCGCC BcgI 5208 CGACCCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4316 GCATTGACTTCG BlpI 4006 GCTCAGC GC^T[ACGT]AGC 6681 GCTCAGC Bpu1102I 4006 GCTCAGC GC^T[ACGT]AGC 6681 GCTCAGC BsaBI 438 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4242 GATCATCATC BspLU11I 5243 ACATGT A^CATGT 5264 ACATGT Bsu36I 2506 CCTGAGG CC^T[ACGT]AGG 6820 CCTGAGG CelII 4006 GCTCAGC GC^T[ACGT]AGC 6681 GCTCAGC Eco47III 6056 AGCGCT AGC^GCT 6374 AGCGCT Eco81I 2506 CCTGAGG CC^T[ACGT]AGG 6820 CCTGAGG EcoNI 6198 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 6816 CCTGCCTGAGG KasI 2845 GGCGCC G^GCGCC 3685 GGCGCC MamI 438 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4242 GATCATCATC NarI 2845 GGCGCC GG^CGCC 3685 GGCGCC PflFI 241 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 4615 GACTTTGTC ScaI 854 AGTACT AGT^ACT 1769 AGTACT SexAI 1046 ACCTGGT A^CC[AT]GGT 1790 ACCTGGT SfoI 2845 GGCGCC GGC^GCC 3685 GGCGCC SgrAI 2755 CACCGGCG C[GA]^CCGG[CT]G 2841 CGCCGGCG Tth111I 241 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 4615 GACTTTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 160 enzymes) # ######################################### Acc65I AccI AccII AccIII AciI AcyI AfaI AflIII AluI AlwI AlwNI ApaI Asp700I Asp718I AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BclI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NotI NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI