Restriction map for pF1KE2582
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AviII       AvrII       BbeI        
BbrPI       BciVI       BclI        BfrI        BglII       
BlnI        BsaAI       BsaBI       BsaHI       BseAI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrDI       BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DraI        
DrdI        Eam1105I    Eco47III    EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
MamI        MfeI        MluI        MroI        MunI        
NarI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SalI        ScaI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SspBI       SspI        StuI        SwaI        
Tth111I     XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I       1088  GGTACC               G^GTACC
AccII        2686  CGCG                 CG^CG
AocI          237  CCTGAGG              CC^T[ACGT]AGG
ApaI          877  GGGCCC               GGGCC^C
Asp718I      1088  GGTACC               G^GTACC
BamHI        2027  GGATCC               G^GATCC
BcgI         2319  GCAGCGATCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         2035  GCCCTGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI         1547  GAGACC               GGTCTC(1/5)
BsaWI         527  TCCGGT               [AT]^CCGG[AT]
BsiEI        2688  CGGCCG               CG[GA][CT]^CG
BsmI         2106  GAATGC               GAATGC(1/-1)
BspHI        1533  TCATGA               T^CATGA
BspMI         898  GCAGGT               ACCTGC(4/8)
BsrFI        2690  GCCGGC               [GA]^CCGG[CT]
BssSI        1027  CACGAG               CACGAG(-5/-1)
BstAPI       2113  GCAGCCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1003  GGTGACC              G^GT[ACGT]ACC
BstPI        1003  GGTGACC              G^GT[ACGT]ACC
BstUI        2686  CGCG                 CG^CG
Bsu36I        237  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       2690  GCCGGC               [GA]^CCGG[CT]
DraIII        493  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         2688  CGGCCG               C^GGCCG
EclXI        2688  CGGCCG               C^GGCCG
Eco52I       2688  CGGCCG               C^GGCCG
Eco81I        237  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1740  CCTGGACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1003  GGTGACC              G^GT[ACGT]ACC
EcoRI        1654  GAATTC               G^AATTC
HaeII         744  GGCGCT               [GA]GCGC^[CT]
KpnI         1088  GGTACC               GGTAC^C
KspI         2685  CCGCGG               CCGC^GG
MvnI         2686  CGCG                 CG^CG
NaeI         2690  GCCGGC               GCC^GGC
NdeI         1811  CATATG               CA^TATG
NgoMIV       2690  GCCGGC               G^CCGGC
RcaI         1533  TCATGA               T^CATGA
SacII        2685  CCGCGG               CCGC^GG
SapI         2652  GCTCTTC              GCTCTTC(1/4)
SbfI          895  CCTGCAGG             CCTGCA^GG
SexAI         494  ACCAGGT              A^CC[AT]GGT
SfiI         2034  GGCCCTGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          434  CTCAAG               C^T[CT][GA]AG
SphI         2517  GCATGC               GCATG^C
Sse8387I      895  CCTGCAGG             CCTGCA^GG
Tsp45I       1004  GTGAC                ^GT[GC]AC
XbaI           93  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI          454  GTCTAC               GT^[AC][GT]AC
             1464  GTCTAC              
AcsI         1433  GAATTT               [GA]^AATT[CT]
             1654  GAATTC              
ApoI         1433  GAATTT               [GA]^AATT[CT]
             1654  GAATTC              
BfaI           94  CTAG                 C^TAG
             1564  CTAG                
BlpI          780  GCTCAGC              GC^T[ACGT]AGC
             2694  GCTGAGC             
Bpu1102I      780  GCTCAGC              GC^T[ACGT]AGC
             2694  GCTGAGC             
BstXI        1008  CCAGCACCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1281  CCAGCAGCTTGG        
CelII         780  GCTCAGC              GC^T[ACGT]AGC
             2694  GCTGAGC             
EcoT14I       645  CCATGG               C^C[AT][AT]GG
             2778  CCATGG              
MaeI           94  CTAG                 C^TAG
             1564  CTAG                
MaeII         770  ACGT                 A^CGT
             2364  ACGT                
MslI         1948  CATGGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2512  CATGAGCATG          
NcoI          645  CCATGG               C^CATGG
             2778  CCATGG              
PflMI        1026  CCACGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1323  CCAGCAAATGG         
PleI          100  GACTC                GAGTC(4/5)
             2350  GACTC               
SacI         2181  GAGCTC               GAGCT^C
             2650  GAGCTC              
StyI          645  CCATGG               C^C[AT][AT]GG
             2778  CCATGG              
TaqI         1560  TCGA                 T^CGA
             1733  TCGA                
TfiI          791  GAATC                G^A[AT]TC
             1480  GAATC               
TthHB8I      1560  TCGA                 T^CGA
             1733  TCGA                
Van91I       1026  CCACGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1323  CCAGCAAATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrBI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        Tru9I       TseI        
Tsp509I     TspRI       XcmI        XhoII       
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