################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AclI AcyI AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BclI BlnI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspDI BspHI BsrFI BssHII Bst1107I BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV FbaI FseI FspI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SfiI SfoI SgrAI SnaBI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 3073 GTCTAC GT^[AC][GT]AC AccII 2085 CGCG CG^CG AflII 2665 CTTAAG C^TTAAG AflIII 197 ACATGT A^C[GA][CT]GT BciVI 2336 GGATAC GTATCC(6/5) BfrI 2665 CTTAAG C^TTAAG BglII 3090 AGATCT A^GATCT Bsp1407I 41 TGTACA T^GTACA BspLU11I 197 ACATGT A^CATGT BsrBI 2272 CCGCTC CCGCTC(-3/-3) BsrGI 41 TGTACA T^GTACA BstAPI 3173 GCATGCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 65 TTCGAA TT^CGAA BstEII 550 GGTGACC G^GT[ACGT]ACC BstPI 550 GGTGACC G^GT[ACGT]ACC BstUI 2085 CGCG CG^CG EcoO65I 550 GGTGACC G^GT[ACGT]ACC EcoRI 3535 GAATTC G^AATTC HaeII 3138 AGCGCC [GA]GCGC^[CT] HincII 19 GTCAAC GT[CT]^[GA]AC HindII 19 GTCAAC GT[CT]^[GA]AC HindIII 2298 AAGCTT A^AGCTT MaeII 1276 ACGT A^CGT MvnI 2085 CGCG CG^CG NcoI 908 CCATGG C^CATGG NspV 65 TTCGAA TT^CGAA PflMI 2196 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1578 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1882 CTGCAG CTGCA^G SapI 557 GAAGAGC GCTCTTC(1/4) SbfI 1881 CCTGCAGG CCTGCA^GG ScaI 235 AGTACT AGT^ACT SexAI 3596 ACCTGGT A^CC[AT]GGT SfuI 65 TTCGAA TT^CGAA SphI 3173 GCATGC GCATG^C Sse8387I 1881 CCTGCAGG CCTGCA^GG SspBI 41 TGTACA T^GTACA SspI 3156 AATATT AAT^ATT StuI 1850 AGGCCT AGG^CCT Van91I 2196 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccIII 1527 TCCGGA T^CCGGA 2818 TCCGGA Alw44I 3564 GTGCAC G^TGCAC 3601 GTGCAC AlwI 1702 GATCC GGATC(4/5) 504 GGATC ApaLI 3564 GTGCAC G^TGCAC 3601 GTGCAC BalI 2450 TGGCCA TGG^CCA 2697 TGGCCA BanI 1640 GGCACC G^G[CT][GA]CC 3288 GGCACC BbsI 1291 GAAGAC GAAGAC(2/6) 1956 GAAGAC BlpI 1159 GCTGAGC GC^T[ACGT]AGC 3549 GCTCAGC Bpu1102I 1159 GCTGAGC GC^T[ACGT]AGC 3549 GCTCAGC BpuAI 1291 GAAGAC GAAGAC(2/6) 1956 GAAGAC BseAI 1527 TCCGGA T^CCGGA 2818 TCCGGA BsmI 323 GCATTC GAATGC(1/-1) 1686 GCATTC BspEI 1527 TCCGGA T^CCGGA 2818 TCCGGA BssSI 1674 CACGAG CACGAG(-5/-1) 3519 CACGAG BstYI 1701 AGATCC [GA]^GATC[CT] 3090 AGATCT CelII 1159 GCTGAGC GC^T[ACGT]AGC 3549 GCTCAGC EcoT22I 3171 ATGCAT ATGCA^T 3300 ATGCAT MflI 1701 AGATCC [GA]^GATC[CT] 3090 AGATCT MluNI 2450 TGGCCA TGG^CCA 2697 TGGCCA MroI 1527 TCCGGA T^CCGGA 2818 TCCGGA MscI 2450 TGGCCA TGG^CCA 2697 TGGCCA NsiI 3171 ATGCAT ATGCA^T 3300 ATGCAT SacI 2040 GAGCTC GAGCT^C 2427 GAGCTC SfcI 1882 CTGCAG C^T[GA][CT]AG 3086 CTGTAG SpeI 346 ACTAGT A^CTAGT 2182 ACTAGT XhoII 1701 AGATCC [GA]^GATC[CT] 3090 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApaI ApoI Asp700I AspHI AvaI AvaII BanII BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XmaCI XmaI XmnI