Restriction map for pF1KE2549
##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

Acc65I      AclI        AcsI        AflII       AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       AvrII       BciVI       BclI        BfrI        
BlnI        BsiWI       BspDI       BsrBI       Bst1107I    
BstBI       BstXI       BstZ17I     ClaI        CpoI        
DraI        DrdI        Eam1105I    Eco47III    EcoRI       
EcoT22I     FbaI        FspI        HindIII     HpaI        
KpnI        MfeI        MluI        MseI        MunI        
NdeI        NheI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SalI        
ScaI        SexAI       SfuI        SnaBI       SpeI        
SspI        SwaI        Tru9I       Tsp509I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI         2911  GTCTAC               GT^[AC][GT]AC
AccIII       1229  TCCGGA               T^CCGGA
AflIII        351  ACATGT               A^C[GA][CT]GT
Asp700I      1500  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          385  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         1656  GGCGCC               GGCGC^C
BfaI          829  CTAG                 C^TAG
BlpI         3667  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3667  GCTGAGC              GC^T[ACGT]AGC
BsaBI         966  GATCCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        1229  TCCGGA               T^CCGGA
Bsp1407I     4271  TGTACA               T^GTACA
BspEI        1229  TCCGGA               T^CCGGA
BspLU11I      351  ACATGT               A^CATGT
BsrGI        4271  TGTACA               T^GTACA
BssHII       1646  GCGCGC               G^CGCGC
BstEII        516  GGTGACC              G^GT[ACGT]ACC
BstPI         516  GGTGACC              G^GT[ACGT]ACC
CelII        3667  GCTGAGC              GC^T[ACGT]AGC
EcoO65I       516  GGTGACC              G^GT[ACGT]ACC
EcoRV        1222  GATATC               GAT^ATC
FseI         3050  GGCCGGCC             GGCCGG^CC
HincII       3463  GTTGAC               GT[CT]^[GA]AC
HindII       3463  GTTGAC               GT[CT]^[GA]AC
KasI         1656  GGCGCC               G^GCGCC
MaeI          829  CTAG                 C^TAG
MamI          966  GATCCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1229  TCCGGA               T^CCGGA
NaeI         3051  GCCGGC               GCC^GGC
NarI         1656  GGCGCC               GG^CGCC
NgoMIV       3051  GCCGGC               G^CCGGC
NotI         3847  GCGGCCGC             GC^GGCCGC
PflFI         385  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacI          703  GAGCTC               GAGCT^C
SbfI         2651  CCTGCAGG             CCTGCA^GG
SfiI         2850  GGCCAAGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1656  GGCGCC               GGC^GCC
SgrAI         633  CACCGGCG             C[GA]^CCGG[CT]G
SmlI         3325  CTCAAG               C^T[CT][GA]AG
SphI         2276  GCATGC               GCATG^C
Sse8387I     2651  CCTGCAGG             CCTGCA^GG
SspBI        4271  TGTACA               T^GTACA
Tth111I       385  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         1500  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

ApaI         2761  GGGCCC               GGGCC^C
             4154  GGGCCC              
BamHI        1626  GGATCC               G^GATCC
             4211  GGATCC              
BbrPI        1678  CACGTG               CAC^GTG
             3715  CACGTG              
BcgI         1080  CGACGTCATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1190  GCAGGATTGTCG        
BsaI         1174  GAGACC               GGTCTC(1/5)
               87  GGTCTC              
BsmBI        3793  GAGACG               CGTCTC(1/5)
             4321  GAGACG              
BsmI          211  GAATGC               GAATGC(1/-1)
             3720  GAATGC              
BspHI         470  TCATGA               T^CATGA
              641  TCATGA              
BsrDI        1086  CATTGC               GCAATG(2/0)
             1398  CATTGC              
BssSI        1345  CACGAG               CACGAG(-5/-1)
              856  CTCGTG              
BstAPI       1302  GCAGGCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1608  GCAGAAGGTGC         
EagI         3225  CGGCCG               C^GGCCG
             3848  CGGCCG              
EclXI        3225  CGGCCG               C^GGCCG
             3848  CGGCCG              
Eco52I       3225  CGGCCG               C^GGCCG
             3848  CGGCCG              
NspI          351  ACATGT               [GA]CATG^[CT]
             2276  GCATGC              
PflMI        2219  CCAAGGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4415  CCATCCGATGG         
PmaCI        1678  CACGTG               CAC^GTG
             3715  CACGTG              
PmlI         1678  CACGTG               CAC^GTG
             3715  CACGTG              
RcaI          470  TCATGA               T^CATGA
              641  TCATGA              
Van91I       2219  CCAAGGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4415  CCATCCGATGG         


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AatII       AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvCI       BbvI        BcnI        BglI        
BglII       BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaJI       BsaWI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     KspI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       RsaI        SacII       SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StuI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     XcmI        
XhoII       XmaCI       XmaI        
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