Restriction map for pF1KE2549
 (Linear) (Six-base) MAPSORT of: KIEE2549.seq  Check: 437  from: 1  to: 4509



KIEE2549.orf

 With 182 enzymes: SgfI * 

                        August 16, 2021 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2155    3150    3275    3537    4416    4509 
   Size:       2155     995     125     262     879      93

  Fragments arranged by size: 

                2155     995     879     262     125      93
 
AatII G_ACGT'C

Cuts at:      0     497    1085    2618    2801    3626    4509 
   Size:        497     588    1533     183     825     883

  Fragments arranged by size: 

                1533     883     825     588     497     183
 
AccI GT'mk_AC

Cuts at:      0    2912    4509 
   Size:       2912    1597
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     577     657    1427    1549    1996    2301    2574    3316 
   Size:        577      80     770     122     447     305     273     742

Cuts at:   3316    3403    3520    3591    4225    4273    4509 
   Size:         87     117      71     634      48     236

  Fragments arranged by size: 

                 770     742     634     577     447     305     273     236
                 122     117      87      80      71      48
 
AflIII A'CryG_T

Cuts at:      0     351    4509 
   Size:        351    4158
 
AleI CACnn'nnGTG

Cuts at:      0    2119    4509 
   Size:       2119    2390
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4012    4509 
   Size:       4012     497
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2395    2427    2881    2913    3784    3816    4509 
   Size:       2395      32     454      32     871      32     693

  Fragments arranged by size: 

                2395     871     693     454      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1146    1245    1692    2109    4489    4509 
   Size:       1146      99     447     417    2380      20

  Fragments arranged by size: 

                2380    1146     447     417      99      20
 
ApaI G_GGCC'C

Cuts at:      0    2765    4158    4509 
   Size:       2765    1393     351

  Fragments arranged by size: 

                2765    1393     351
 
AvaI C'yCGr_G

Cuts at:      0     999    1065    1630    2680    2946    3025    3430    3563 
   Size:        999      66     565    1050     266      79     405     133

Cuts at:   3563    4172    4293    4404    4509 
   Size:        609     121     111     105

  Fragments arranged by size: 

                1050     999     609     565     405     266     133     121
                 111     105      79      66
 
BamHI G'GATC_C

Cuts at:      0    1626    4211    4509 
   Size:       1626    2585     298

  Fragments arranged by size: 

                2585    1626     298
 
BanI G'GyrC_C

Cuts at:      0    1039    1656    2344    2998    3728    3783    4509 
   Size:       1039     617     688     654     730      55     726

  Fragments arranged by size: 

                1039     730     726     688     654     617      55
 
BanII G_rGCy'C

Cuts at:      0       8     707    1066    1929    2103    2765    3514    3570 
   Size:          8     699     359     863     174     662     749      56

Cuts at:   3570    4158    4171    4503    4509 
   Size:        588      13     332       6

  Fragments arranged by size: 

                 863     749     699     662     588     359     332     174
                  56      13       8       6
 
BbeI G_GCGC'C

Cuts at:      0    1660    4509 
   Size:       1660    2849
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     384    1245    2058    2146    2385    2793    3304    3694 
   Size:        384     861     813      88     239     408     511     390

Cuts at:   3694    4509 
   Size:        815

  Fragments arranged by size: 

                 861     815     813     511     408     390     384     239
                  88
 
BbvCI CC'TCA_GC

Cuts at:      0       9    3259    4015    4509 
   Size:          9    3250     756     494

  Fragments arranged by size: 

                3250     756     494       9
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1069    1103    1179    1213    4509 
   Size:       1069      34      76      34    3296

  Fragments arranged by size: 

                3296    1069      76      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     149     752    1096    2167    2857    2996    4509 
   Size:        149     603     344    1071     690     139    1513

  Fragments arranged by size: 

                1513    1071     690     603     344     149     139
 
BglII A'GATC_T

Cuts at:      0    1412    1446    3374    4509 
   Size:       1412      34    1928    1135

  Fragments arranged by size: 

                1928    1412    1135      34
 
BlpI GC'TnA_GC

Cuts at:      0    3668    4509 
   Size:       3668     841
 
Bme1580I G_kGCm'C

Cuts at:      0     145     331     736    1006    2347    2474    2765    3025 
   Size:        145     186     405     270    1341     127     291     260

Cuts at:   3025    3773    3788    4158    4509 
   Size:        748      15     370     351

  Fragments arranged by size: 

                1341     748     405     370     351     291     270     260
                 186     145     127      15
 
BmgBI CAC'GTC

Cuts at:      0    1284    1782    4362    4509 
   Size:       1284     498    2580     147

  Fragments arranged by size: 

                2580    1284     498     147
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     492    1362    4161    4509 
   Size:        492     870    2799     348

  Fragments arranged by size: 

                2799     870     492     348
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1825    4509 
   Size:       1825    2684
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     435     934    1009    1531    2185    3358    3382 
   Size:        150     285     499      75     522     654    1173      24

Cuts at:   3382    3559    3898    4459    4509 
   Size:        177     339     561      50

  Fragments arranged by size: 

                1173     654     561     522     499     339     285     177
                 150      75      50      24
 
Bpu10I CC'TnA_GC

Cuts at:      0       9     403     679    3259    4015    4509 
   Size:          9     394     276    2580     756     494

  Fragments arranged by size: 

                2580     756     494     394     276       9
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3310    4509 
   Size:       3310    1199
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      93    1168    4509 
   Size:         93    1075    3341

  Fragments arranged by size: 

                3341    1075      93
 
BsaAI yAC'GTr

Cuts at:      0    1680    2445    3717    4509 
   Size:       1680     765    1272     792

  Fragments arranged by size: 

                1680    1272     792     765
 
BsaBI GATnn'nnATC

Cuts at:      0     970    4509 
   Size:        970    3539
 
BsaHI Gr'CG_yC

Cuts at:      0     494     638    1082    1657    2615    2798    3467    3623 
   Size:        494     144     444     575     958     183     669     156

Cuts at:   3623    4509 
   Size:        886

  Fragments arranged by size: 

                 958     886     669     575     494     444     183     156
                 144
 
BsaWI w'CCGG_w

Cuts at:      0    1229    1811    2081    4509 
   Size:       1229     582     270    2428

  Fragments arranged by size: 

                2428    1229     582     270
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     433     463    2395    2425    3511    3541    3784    3814 
   Size:        433      30    1932      30    1086      30     243      30

Cuts at:   3814    3871    3901    4509 
   Size:         57      30     608

  Fragments arranged by size: 

                1932    1086     608     433     243      57      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      55     313     962    2008    2011    2032    2053    2287 
   Size:         55     258     649    1046       3      21      21     234

Cuts at:   2287    2539    3118    3229    3247    3364    3556    3670    3709 
   Size:        252     579     111      18     117     192     114      39

Cuts at:   3709    3724    4462    4509 
   Size:         15     738      47

  Fragments arranged by size: 

                1046     738     649     579     258     252     234     192
                 117     114     111      55      47      39      21      21
                  18      15       3
 
BseYI C'CCAG_C

Cuts at:      0     250     476    1704    2757    4509 
   Size:        250     226    1228    1053    1752

  Fragments arranged by size: 

                1752    1228    1053     250     226
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1435    1454    1543    1816    3184    3556    3976    4375 
   Size:       1435      19      89     273    1368     372     420     399

Cuts at:   4375    4509 
   Size:        134

  Fragments arranged by size: 

                1435    1368     420     399     372     273     134      89
                  19
 
BsiEI CG_ry'CG

Cuts at:      0    1752    3228    3851    4509 
   Size:       1752    1476     623     658

  Fragments arranged by size: 

                1752    1476     658     623
 
BsiHKAI G_wGCw'C

Cuts at:      0     707    2051    2450    2984    3173    4151    4229    4509 
   Size:        707    1344     399     534     189     978      78     280

  Fragments arranged by size: 

                1344     978     707     534     399     280     189      78
            
 
BsmI GAATG_Cn'

Cuts at:      0     217    3726    4509 
   Size:        217    3509     783

  Fragments arranged by size: 

                3509     783     217
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3787    4315    4509 
   Size:       3787     528     194

  Fragments arranged by size: 

                3787     528     194
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     145     331     707     736    1006    1066    1929 
   Size:          8     137     186     376      29     270      60     863

Cuts at:   1929    2051    2103    2347    2450    2474    2765    2984    3025 
   Size:        122      52     244     103      24     291     219      41

Cuts at:   3025    3173    3514    3570    3773    3788    4151    4158    4171 
   Size:        148     341      56     203      15     363       7      13

Cuts at:   4171    4229    4503    4509 
   Size:         58     274       6

  Fragments arranged by size: 

                 863     376     363     341     291     274     270     244
                 219     203     186     148     137     122     103      60
                  58      56      52      41      29      24      15      13
                   8       7       6
 
BspEI T'CCGG_A

Cuts at:      0    1229    4509 
   Size:       1229    3280
 
BspHI T'CATG_A

Cuts at:      0     470     641    4509 
   Size:        470     171    3868

  Fragments arranged by size: 

                3868     470     171
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     639     832    1112    2155    2659    3104    3150    3275 
   Size:        639     193     280    1043     504     445      46     125

Cuts at:   3275    3488    3537    4397    4416    4509 
   Size:        213      49     860      19      93

  Fragments arranged by size: 

                1043     860     639     504     445     280     213     193
                 125      93      49      46      19
 
BsrDI GCAATG_nn'

Cuts at:      0    1085    1397    4509 
   Size:       1085     312    3112

  Fragments arranged by size: 

                3112    1085     312
 
BsrFI r'CCGG_y

Cuts at:      0     634    1751    2302    3051    4509 
   Size:        634    1117     551     749    1458

  Fragments arranged by size: 

                1458    1117     749     634     551
 
BsrGI T'GTAC_A

Cuts at:      0    4271    4509 
   Size:       4271     238
 
BssHII G'CGCG_C

Cuts at:      0    1646    4509 
   Size:       1646    2863
 
BssSI C'ACGA_G

Cuts at:      0     856    1345    4509 
   Size:        856     489    3164

  Fragments arranged by size: 

                3164     856     489
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1308    1614    4509 
   Size:       1308     306    2895

  Fragments arranged by size: 

                2895    1308     306
 
BstEII G'GTnAC_C

Cuts at:      0     516    4509 
   Size:        516    3993
 
BstYI r'GATC_y

Cuts at:      0     965    1214    1412    1446    1626    1970    2942    3374 
   Size:        965     249     198      34     180     344     972     432

Cuts at:   3374    4211    4509 
   Size:        837     298

  Fragments arranged by size: 

                 972     965     837     432     344     298     249     198
                 180      34
 
Bsu36I CC'TnA_GG

Cuts at:      0      16    1833    3919    4509 
   Size:         16    1817    2086     590

  Fragments arranged by size: 

                2086    1817     590      16
 
BtgI C'CryG_G

Cuts at:      0     550     767     873    1652    1853    2243    2351    2384 
   Size:        550     217     106     779     201     390     108      33

Cuts at:   2384    3222    3470    3515    3650    3854    3863    3939    3980 
   Size:        838     248      45     135     204       9      76      41

Cuts at:   3980    4049    4509 
   Size:         69     460

  Fragments arranged by size: 

                 838     779     550     460     390     248     217     204
                 201     135     108     106      76      69      45      41
                  33       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1929    3117    4509 
   Size:       1929    1188    1392

  Fragments arranged by size: 

                1929    1392    1188
 
BtsI GCAGTG_nn'

Cuts at:      0    1382    1413    1438    2982    4509 
   Size:       1382      31      25    1544    1527

  Fragments arranged by size: 

                1544    1527    1382      31      25
 
DraIII CAC_nnn'GTG

Cuts at:      0     335    1680    2075    4061    4509 
   Size:        335    1345     395    1986     448

  Fragments arranged by size: 

                1986    1345     448     395     335
 
EaeI y'GGCC_r

Cuts at:      0     807     876     956    1076    1097    1292    1341    2849 
   Size:        807      69      80     120      21     195      49    1508

Cuts at:   2849    3225    3305    3848    4231    4328    4509 
   Size:        376      80     543     383      97     181

  Fragments arranged by size: 

                1508     807     543     383     376     195     181     120
                  97      80      80      69      49      21
 
EagI C'GGCC_G

Cuts at:      0    3225    3848    4509 
   Size:       3225     623     661

  Fragments arranged by size: 

                3225     661     623
 
EarI CTCTTCn'nnn_

Cuts at:      0     124     553    1510    2087    2320    2512    2545    2638 
   Size:        124     429     957     577     233     192      33      93

Cuts at:   2638    2818    3106    3280    3496    4090    4509 
   Size:        180     288     174     216     594     419

  Fragments arranged by size: 

                 957     594     577     429     419     288     233     216
                 192     180     174     124      93      33
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2050    3634    4083    4509 
   Size:       2050    1584     449     426

  Fragments arranged by size: 

                2050    1584     449     426
 
EcoICRI GAG'CTC

Cuts at:      0     705    4509 
   Size:        705    3804
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     435     577     657     934    1009    1427    1531 
   Size:        150     285     142      80     277      75     418     104

Cuts at:   1531    1549    1996    2185    2301    2574    3316    3358    3382 
   Size:         18     447     189     116     273     742      42      24

Cuts at:   3382    3403    3520    3559    3591    3898    4225    4273    4459 
   Size:         21     117      39      32     307     327      48     186

Cuts at:   4459    4509 
   Size:         50

  Fragments arranged by size: 

                 742     447     418     327     307     285     277     273
                 189     186     150     142     117     116     104      80
                  75      50      48      42      39      32      24      21
                  18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     583    2218    3211    4219    4477    4509 
   Size:        583    1635     993    1008     258      32

  Fragments arranged by size: 

                1635    1008     993     583     258      32
 
EcoO109I rG'GnC_Cy

Cuts at:      0     339     605     626     753     869    2761    4155    4409 
   Size:        339     266      21     127     116    1892    1394     254

Cuts at:   4409    4509 
   Size:        100

  Fragments arranged by size: 

                1892    1394     339     266     254     127     116     100
                  21
 
EcoRV GAT'ATC

Cuts at:      0    1224    4509 
   Size:       1224    3285
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2598    2817    4509 
   Size:       2598     219    1692

  Fragments arranged by size: 

                2598    1692     219
 
FseI GG_CCGG'CC

Cuts at:      0    3055    4509 
   Size:       3055    1454
 
HaeII r_GCGC'y

Cuts at:      0    1638    1660    2032    2883    3201    4509 
   Size:       1638      22     372     851     318    1308

  Fragments arranged by size: 

                1638    1308     851     372     318      22
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     433     465     566     598    2894    2926    3871    3903 
   Size:        433      32     101      32    2296      32     945      32

Cuts at:   3903    4179    4211    4509 
   Size:        276      32     298

  Fragments arranged by size: 

                2296     945     433     298     276     101      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    3465    4509 
   Size:       3465    1044
 
KasI G'GCGC_C

Cuts at:      0    1656    4509 
   Size:       1656    2853
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1505    4509 
   Size:       1505    3004
 
MscI TGG'CCA

Cuts at:      0     809     958    1099    1294    1343    2851    4509 
   Size:        809     149     141     195      49    1508    1658

  Fragments arranged by size: 

                1658    1508     809     195     149     141      49
 
MslI CAynn'nnrTG

Cuts at:      0     856    2119    2776    3136    3719    4267    4509 
   Size:        856    1263     657     360     583     548     242

  Fragments arranged by size: 

                1263     856     657     583     548     360     242
 
MspA1I CmG'CkG

Cuts at:      0     552    1242    1365    1468    1527    1654    1951    2292 
   Size:        552     690     123     103      59     127     297     341

Cuts at:   2292    2353    3426    3602    3652    4019    4489    4509 
   Size:         61    1073     176      50     367     470      20

  Fragments arranged by size: 

                1073     690     552     470     367     341     297     176
                 127     123     103      61      59      50      20
 
NaeI GCC'GGC

Cuts at:      0    3053    4509 
   Size:       3053    1456
 
NarI GG'CG_CC

Cuts at:      0    1657    4509 
   Size:       1657    2852
 
NcoI C'CATG_G

Cuts at:      0    1853    3470    3515    3854    3863    3980    4049    4509 
   Size:       1853    1617      45     339       9     117      69     460

  Fragments arranged by size: 

                1853    1617     460     339     117      69      45       9
            
 
NgoMIV G'CCGG_C

Cuts at:      0    3051    4509 
   Size:       3051    1458
 
NotI GC'GGCC_GC

Cuts at:      0    3848    4509 
   Size:       3848     661
 
NspI r_CATG'y

Cuts at:      0     355    2280    4509 
   Size:        355    1925    2229

  Fragments arranged by size: 

                2229    1925     355
 
PasI CC'CwG_GG

Cuts at:      0    3788    4509 
   Size:       3788     721
 
PciI A'CATG_T

Cuts at:      0     351    4509 
   Size:        351    4158
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2225    4421    4509 
   Size:       2225    2196      88

  Fragments arranged by size: 

                2225    2196      88
 
PfoI T'CCnGG_A

Cuts at:      0     356    1160    1256    1508    4346    4509 
   Size:        356     804      96     252    2838     163

  Fragments arranged by size: 

                2838     804     356     252     163      96
 
PmlI CAC'GTG

Cuts at:      0    1680    3717    4509 
   Size:       1680    2037     792

  Fragments arranged by size: 

                2037    1680     792
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2395    2427    3784    3816    4509 
   Size:       2395      32    1357      32     693

  Fragments arranged by size: 

                2395    1357     693      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     339     626     869    4509 
   Size:        339     287     243    3640

  Fragments arranged by size: 

                3640     339     287     243
 
PspOMI G'GGCC_C

Cuts at:      0    2761    4154    4509 
   Size:       2761    1393     355

  Fragments arranged by size: 

                2761    1393     355
 
PstI C_TGCA'G

Cuts at:      0    1610    1670    1966    2156    2336    2656    2989    3272 
   Size:       1610      60     296     190     180     320     333     283

Cuts at:   3272    4093    4509 
   Size:        821     416

  Fragments arranged by size: 

                1610     821     416     333     320     296     283     190
                 180      60
 
PvuII CAG'CTG

Cuts at:      0    1242    1365    1468    1527    4019    4509 
   Size:       1242     123     103      59    2492     490

  Fragments arranged by size: 

                2492    1242     490     123     103      59
 
SacI G_AGCT'C

Cuts at:      0     707    4509 
   Size:        707    3802
 
SacII CC_GC'GG

Cuts at:      0     553    1655    2354    3653    4509 
   Size:        553    1102     699    1299     856

  Fragments arranged by size: 

                1299    1102     856     699     553
 
SanDI GG'GwC_CC

Cuts at:      0     339     626     869    4509 
   Size:        339     287     243    3640

  Fragments arranged by size: 

                3640     339     287     243
 
SapI GCTCTTCn'nnn_

Cuts at:      0     553    1510    2512    2545    2638    3106    3280    4090 
   Size:        553     957    1002      33      93     468     174     810

Cuts at:   4090    4509 
   Size:        419

  Fragments arranged by size: 

                1002     957     810     553     468     419     174      93
                  33
 
SbfI CC_TGCA'GG

Cuts at:      0    2656    4509 
   Size:       2656    1853
 
SfcI C'TryA_G

Cuts at:      0     136    1606    1666    1962    2152    2332    2652    2985 
   Size:        136    1470      60     296     190     180     320     333

Cuts at:   2985    3268    4089    4509 
   Size:        283     821     420

  Fragments arranged by size: 

                1470     821     420     333     320     296     283     190
                 180     136      60
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2857    4509 
   Size:       2857    1652
 
SfoI GGC'GCC

Cuts at:      0    1658    4509 
   Size:       1658    2851
 
SgrAI Cr'CCGG_yG

Cuts at:      0     634    4509 
   Size:        634    3875
 
SmaI CCC'GGG

Cuts at:      0    1001    2682    2948    4174    4509 
   Size:       1001    1681     266    1226     335

  Fragments arranged by size: 

                1681    1226    1001     335     266
 
SmlI C'TyrA_G

Cuts at:      0    3325    4509 
   Size:       3325    1184
 
SphI G_CATG'C

Cuts at:      0    2280    4509 
   Size:       2280    2229
 
SrfI GCCC'GGGC

Cuts at:      0    1001    4509 
   Size:       1001    3508
 
StuI AGG'CCT

Cuts at:      0     578    1306    2131    2209    4509 
   Size:        578     728     825      78    2300

  Fragments arranged by size: 

                2300     825     728     578      78
 
StyI C'CwwG_G

Cuts at:      0     810     938    1853    1955    1988    2171    2219    2513 
   Size:        810     128     915     102      33     183      48     294

Cuts at:   2513    3470    3515    3854    3863    3980    4049    4509 
   Size:        957      45     339       9     117      69     460

  Fragments arranged by size: 

                 957     915     810     460     339     294     183     128
                 117     102      69      48      45      33       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2820    4509 
   Size:       2820    1689
 
TatI w'GTAC_w

Cuts at:      0     788    2585    3899    4271    4509 
   Size:        788    1797    1314     372     238

  Fragments arranged by size: 

                1797    1314     788     372     238
 
Tth111I GACn'n_nGTC

Cuts at:      0     388    4509 
   Size:        388    4121
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     978    1336    3861    4509 
   Size:        978     358    2525     648

  Fragments arranged by size: 

                2525     978     648     358
 
XmaI C'CCGG_G

Cuts at:      0     999    2680    2946    4172    4509 
   Size:        999    1681     266    1226     337

  Fragments arranged by size: 

                1681    1226     999     337     266
 
XmnI GAAnn'nnTTC

Cuts at:      0    1504    4509 
   Size:       1504    3005
 
ZraI GAC'GTC

Cuts at:      0     495    1083    2616    2799    3624    4509 
   Size:        495     588    1533     183     825     885

  Fragments arranged by size: 

                1533     885     825     588     495     183
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AleI     AlfI     AloI
    AlwNI     ApaI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI   BstEII    BstYI   Bsu36I     BtgI    BtgZI
     BtsI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRV     FalI     FseI    HaeII    Hin4I   HincII
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NotI     NspI     PasI     PciI    PflMI     PfoI     PmlI
     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII    SanDI
     SapI     SbfI     SfcI     SfiI     SfoI    SgrAI     SmaI     SmlI
     SphI     SrfI     StuI     StyI    TaqII     TatI  Tth111I     XcmI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BciVI     BclI    BfrBI
     BmtI    BsiWI    BsrBI    BstBI    BstXI  BstZ17I     ClaI    CspCI
     DraI     DrdI    EcoRI     FspI    FspAI  HindIII     HpaI     KpnI
     MfeI     MluI     NdeI     NheI     NruI     NsiI     PacI     PmeI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI     ScaI
    SexAI     SgfI    SnaBI     SpeI     SspI     SwaI     TstI     XbaI
     XhoI
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