Restriction map for
pF1KE2114
(Circular) (Six-base) MAPSORT of: pF1KE2114.seq Check: 242 from: 1 to: 5759
pF1KE2114 5759 bp
With 182 enzymes: SgfI *
September 11, 2017 11:55 ..
AccI GT'mk_AC
Cuts at: 294 407 1129 1343 2797 294
Size: 113 722 214 1454 3256
Fragments arranged by size:
3256 1454 722 214 113
Acc65I G'GTAC_C
Cuts at: 1485 2775 1485
Size: 1290 4469
AclI AA'CG_TT
Cuts at: 2360 5599 2360
Size: 3239 2520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 2468 3584 4016 4763 5031 769
Size: 1699 1116 432 747 268 1497
Fragments arranged by size:
1699 1497 1116 747 432 268
AflIII A'CryG_T
Cuts at: 218 1464 2729 3111 4215 5192 218
Size: 1246 1265 382 1104 977 785
Fragments arranged by size:
1265 1246 1104 977 785 382
AgeI A'CCGG_T
Cuts at: 289 4147 289
Size: 3858 1901
AhdI GACnn_n'nnGTC
Cuts at: 1375 1375
Size: 5759
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2246 2246
Size: 5759
AlwNI CAG_nnn'CTG
Cuts at: 1204 3064 4631 1204
Size: 1860 1567 2332
Fragments arranged by size:
2332 1860 1567
ApaI G_GGCC'C
Cuts at: 115 115
Size: 5759
ApaLI G'TGCA_C
Cuts at: 4529 4529
Size: 5759
ApoI r'AATT_y
Cuts at: 273 889 2225 2255 2532 2763 5112 5368 273
Size: 616 1336 30 277 231 2349 256 664
Fragments arranged by size:
2349 1336 664 616 277 256 231 30
AseI AT'TA_AT
Cuts at: 20 1712 20
Size: 1692 4067
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5759
AvaI C'yCGr_G
Cuts at: 1739 2351 2779 1739
Size: 612 428 4719
Fragments arranged by size:
4719 612 428
BamHI G'GATC_C
Cuts at: 837 2784 837
Size: 1947 3812
BanI G'GyrC_C
Cuts at: 228 236 467 1257 1485 1726 2775 3189 3437
Size: 8 231 790 228 241 1049 414 248
Cuts at: 3437 3472 228
Size: 35 2515
Fragments arranged by size:
2515 1049 790 414 248 241 231 228
35 8
BanII G_rGCy'C
Cuts at: 115 188 1040 2327 2647 2773 3803 115
Size: 73 852 1287 320 126 1030 2071
Fragments arranged by size:
2071 1287 1030 852 320 126 73
BbeI G_GCGC'C
Cuts at: 240 3193 3441 240
Size: 2953 248 2558
Fragments arranged by size:
2953 2558 248
BbsI GAAGACnn'nnnn_
Cuts at: 564 564
Size: 5759
BbvCI CC'TCA_GC
Cuts at: 1021 1021
Size: 5759
BciVI GTATCCnnnnn_n'
Cuts at: 3657 4418 5759 3657
Size: 761 1341 3657
Fragments arranged by size:
3657 1341 761
BclI T'GATC_A
Cuts at: 854 1193 3279 854
Size: 339 2086 3334
Fragments arranged by size:
3334 2086 339
BglI GCCn_nnn'nGGC
Cuts at: 197 197
Size: 5759
BglII A'GATC_T
Cuts at: 3274 5311 3274
Size: 2037 3722
BlpI GC'TnA_GC
Cuts at: 1745 2871 1745
Size: 1126 4633
Bme1580I G_kGCm'C
Cuts at: 115 233 343 470 3384 3477 4533 115
Size: 118 110 127 2914 93 1056 1341
Fragments arranged by size:
2914 1341 1056 127 118 110 93
BmrI ACTGGGnnnn_n'
Cuts at: 582 1109 2971 3052 3387 5531 582
Size: 527 1862 81 335 2144 810
Fragments arranged by size:
2144 1862 810 527 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4126 4126
Size: 5759
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 1726 1808 265
Size: 1461 82 4216
Fragments arranged by size:
4216 1461 82
Bpu10I CC'TnA_GC
Cuts at: 1021 1034 1021
Size: 13 5746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2092 2433 2935 3814 4306 4604 2092
Size: 341 502 879 492 298 3247
Fragments arranged by size:
3247 879 502 492 341 298
BsaI GGTCTCn'nnnn_
Cuts at: 162 1503 5463 162
Size: 1341 3960 458
Fragments arranged by size:
3960 1341 458
BsaAI yAC'GTr
Cuts at: 1127 1244 1467 1773 1787 3112 1127
Size: 117 223 306 14 1325 3774
Fragments arranged by size:
3774 1325 306 223 117 14
BsaBI GATnn'nnATC
Cuts at: 6 853 2546 3273 5310 6
Size: 847 1693 727 2037 455
Fragments arranged by size:
2037 1693 847 727 455
BsaHI Gr'CG_yC
Cuts at: 206 237 2186 3190 3438 4140 5636 206
Size: 31 1949 1004 248 702 1496 329
Fragments arranged by size:
1949 1496 1004 702 329 248 31
BsaWI w'CCGG_w
Cuts at: 289 1716 2948 3469 4147 4421 4568 4979 289
Size: 1427 1232 521 678 274 147 411 1069
Fragments arranged by size:
1427 1232 1069 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 150 531 2319 2646 150
Size: 381 1788 327 3263
Fragments arranged by size:
3263 1788 381 327
BseYI C'CCAG_C
Cuts at: 1326 3184 3942 4519 5015 1326
Size: 1858 758 577 496 2070
Fragments arranged by size:
2070 1858 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2407 2407
Size: 5759
BsiEI CG_ry'CG
Cuts at: 104 294 3301 3347 4555 104
Size: 190 3007 46 1208 1308
Fragments arranged by size:
3007 1308 1208 190 46
BsiHKAI G_wGCw'C
Cuts at: 933 1040 1772 2647 2773 3551 3741 4533 933
Size: 107 732 875 126 778 190 792 2159
Fragments arranged by size:
2159 875 792 778 732 190 126 107
BsmI GAATG_Cn'
Cuts at: 831 831
Size: 5759
Bsp1286I G_dGCh'C
Cuts at: 115 188 233 343 470 933 1040 1772 2327
Size: 73 45 110 127 463 107 732 555
Cuts at: 2327 2647 2773 3384 3477 3551 3741 3803 4533
Size: 320 126 611 93 74 190 62 730
Cuts at: 4533 115
Size: 1341
Fragments arranged by size:
1341 732 730 611 555 463 320 190
127 126 110 107 93 74 73 62
45
BspEI T'CCGG_A
Cuts at: 1716 4979 1716
Size: 3263 2496
BspHI T'CATG_A
Cuts at: 2480 4935 2480
Size: 2455 3304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 144 2809 3023 3325 3706 144
Size: 2665 214 302 381 2197
Fragments arranged by size:
2665 2197 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2311 4051 4105 5589 5754 50
Size: 2261 1740 54 1484 165 55
Fragments arranged by size:
2261 1740 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 779 3671 779
Size: 2892 2867
BsrFI r'CCGG_y
Cuts at: 289 3757 3938 4147 289
Size: 3468 181 209 1901
Fragments arranged by size:
3468 1901 209 181
BssSI C'ACGA_G
Cuts at: 2599 4030 4388 2599
Size: 1431 358 3970
Fragments arranged by size:
3970 1431 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3136 3136
Size: 5759
BstBI TT'CG_AA
Cuts at: 4120 4120
Size: 5759
BstEII G'GTnAC_C
Cuts at: 861 1092 861
Size: 231 5528
BstXI CCAn_nnnn'nTGG
Cuts at: 1104 1704 1104
Size: 600 5159
BstYI r'GATC_y
Cuts at: 1 392 837 1029 2784 3251 3274 3609 3855
Size: 391 445 192 1755 467 23 335 246
Cuts at: 3855 4867 4953 4965 5311 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1755 1012 467 449 445 391 346 335
246 192 86 23 12
BstZ17I GTA'TAC
Cuts at: 1130 1344 1130
Size: 214 5545
Bsu36I CC'TnA_GG
Cuts at: 1934 1934
Size: 5759
BtgI C'CryG_G
Cuts at: 107 1529 107
Size: 1422 4337
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 990 3709 3890 4053 990
Size: 2719 181 163 2696
Fragments arranged by size:
2719 2696 181 163
BtsI GCAGTG_nn'
Cuts at: 1478 1631 1691 3002 1478
Size: 153 60 1311 4235
Fragments arranged by size:
4235 1311 153 60
DraI TTT'AAA
Cuts at: 2758 2758
Size: 5759
DraIII CAC_nnn'GTG
Cuts at: 810 810
Size: 5759
DrdI GACnn_nn'nnGTC
Cuts at: 3465 4323 3465
Size: 858 4901
EaeI y'GGCC_r
Cuts at: 687 3344 3518 3909 3936 5281 687
Size: 2657 174 391 27 1345 1165
Fragments arranged by size:
2657 1345 1165 391 174 27
EagI C'GGCC_G
Cuts at: 3344 3344
Size: 5759
EarI CTCTTCn'nnn_
Cuts at: 559 802 938 2387 3782 3992 559
Size: 243 136 1449 1395 210 2326
Fragments arranged by size:
2326 1449 1395 243 210 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2402 3967 4277 4423 5569 2402
Size: 1565 310 146 1146 2592
Fragments arranged by size:
2592 1565 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1038 2645 2771 1038
Size: 1607 126 4026
Fragments arranged by size:
4026 1607 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 769 1726 1808 2468 3584 4016 4763 5031
Size: 504 957 82 660 1116 432 747 268
Cuts at: 5031 265
Size: 993
Fragments arranged by size:
1116 993 957 747 660 504 432 268
82
EcoO109I rG'GnC_Cy
Cuts at: 112 2430 2898 112
Size: 2318 468 2973
Fragments arranged by size:
2973 2318 468
EcoRI G'AATT_C
Cuts at: 889 2255 2763 889
Size: 1366 508 3885
Fragments arranged by size:
3885 1366 508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 949 2094 3778 949
Size: 1145 1684 2930
Fragments arranged by size:
2930 1684 1145
FspI TGC'GCA
Cuts at: 3540 3540
Size: 5759
HaeII r_GCGC'y
Cuts at: 139 240 3193 3441 4463 5442 139
Size: 101 2953 248 1022 979 456
Fragments arranged by size:
2953 1022 979 456 248 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 242 274 747 779 1218 1250 1361 1393 2300
Size: 32 473 32 439 32 111 32 907
Cuts at: 2300 2332 3601 3631 3633 3663 242
Size: 32 1269 30 2 30 2338
Fragments arranged by size:
2338 1269 907 473 439 111 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 295 2798 295
Size: 2503 3256
KasI G'GCGC_C
Cuts at: 236 3189 3437 236
Size: 2953 248 2558
Fragments arranged by size:
2953 2558 248
KpnI G_GTAC'C
Cuts at: 1489 2779 1489
Size: 1290 4469
MluI A'CGCG_T
Cuts at: 5192 5192
Size: 5759
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 671 842 4430 4614 671
Size: 171 3588 184 1816
Fragments arranged by size:
3588 1816 184 171
MscI TGG'CCA
Cuts at: 689 3520 689
Size: 2831 2928
MslI CAynn'nnrTG
Cuts at: 1087 1564 1654 3748 3875 1087
Size: 477 90 2094 127 2971
Fragments arranged by size:
2971 2094 477 127 90
MspA1I CmG'CkG
Cuts at: 593 1189 2182 2421 2870 3184 3544 4557 4802
Size: 596 993 239 449 314 360 1013 245
Cuts at: 4802 593
Size: 1550
Fragments arranged by size:
1550 1013 993 596 449 360 314 245
239
NaeI GCC'GGC
Cuts at: 3940 3940
Size: 5759
NarI GG'CG_CC
Cuts at: 237 3190 3438 237
Size: 2953 248 2558
Fragments arranged by size:
2953 2558 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5759
NgoMIV G'CCGG_C
Cuts at: 3938 3938
Size: 5759
NspI r_CATG'y
Cuts at: 2733 2812 3312 4219 2733
Size: 79 500 907 4273
Fragments arranged by size:
4273 907 500 79
PciI A'CATG_T
Cuts at: 2729 4215 2729
Size: 1486 4273
PflMI CCAn_nnn'nTGG
Cuts at: 402 402
Size: 5759
PfoI T'CCnGG_A
Cuts at: 936 936
Size: 5759
PmeI GTTT'AAAC
Cuts at: 2758 2758
Size: 5759
PmlI CAC'GTG
Cuts at: 1773 1773
Size: 5759
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4898 4930 4898
Size: 32 5727
PspOMI G'GGCC_C
Cuts at: 111 111
Size: 5759
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1771 1803 1771
Size: 32 5727
PstI C_TGCA'G
Cuts at: 155 2806 3491 155
Size: 2651 685 2423
Fragments arranged by size:
2651 2423 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5759
PvuII CAG'CTG
Cuts at: 593 1189 2421 3184 3544 593
Size: 596 1232 763 360 2808
Fragments arranged by size:
2808 1232 763 596 360
RsrII CG'GwC_CG
Cuts at: 3954 3954
Size: 5759
SacI G_AGCT'C
Cuts at: 1040 2647 2773 1040
Size: 1607 126 4026
Fragments arranged by size:
4026 1607 126
SalI G'TCGA_C
Cuts at: 293 2796 293
Size: 2503 3256
SapI GCTCTTCn'nnn_
Cuts at: 938 3782 3992 938
Size: 2844 210 2705
Fragments arranged by size:
2844 2705 210
SbfI CC_TGCA'GG
Cuts at: 2806 2806
Size: 5759
ScaI AGT'ACT
Cuts at: 5276 5276
Size: 5759
SexAI A'CCwGG_T
Cuts at: 629 629
Size: 5759
SfcI C'TryA_G
Cuts at: 33 151 426 675 1808 2190 2802 3487 4480
Size: 118 275 249 1133 382 612 685 993
Cuts at: 4480 4671 33
Size: 191 1121
Fragments arranged by size:
1133 1121 993 685 612 382 275 249
191 118
SfoI GGC'GCC
Cuts at: 238 3191 3439 238
Size: 2953 248 2558
Fragments arranged by size:
2953 2558 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5759
SmaI CCC'GGG
Cuts at: 2353 2781 2353
Size: 428 5331
SmlI C'TyrA_G
Cuts at: 2107 2412 2914 3829 4321 4583 2107
Size: 305 502 915 492 262 3283
Fragments arranged by size:
3283 915 502 492 305 262
SnaBI TAC'GTA
Cuts at: 1127 1127
Size: 5759
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5759
SphI G_CATG'C
Cuts at: 2812 3312 2812
Size: 500 5259
StuI AGG'CCT
Cuts at: 369 496 369
Size: 127 5632
StyI C'CwwG_G
Cuts at: 107 115 690 2893 3247 107
Size: 8 575 2203 354 2619
Fragments arranged by size:
2619 2203 575 354 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 910 1124 2077 3342 3475 3934 4143 910
Size: 214 953 1265 133 459 209 2526
Fragments arranged by size:
2526 1265 953 459 214 209 133
TatI w'GTAC_w
Cuts at: 1454 2030 5274 1454
Size: 576 3244 1939
Fragments arranged by size:
3244 1939 576
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 932 964 1374 1406 5276 5308 932
Size: 32 410 32 3870 32 1383
Fragments arranged by size:
3870 1383 410 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3556 3556
Size: 5759
XbaI T'CTAG_A
Cuts at: 2790 2790
Size: 5759
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1527 3254 1527
Size: 1727 4032
XmaI C'CCGG_G
Cuts at: 2351 2779 2351
Size: 428 5331
XmnI GAAnn'nnTTC
Cuts at: 435 2622 435
Size: 2187 3572
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI
BsrGI BssHII ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PpuMI PshAI PsiI PspXI SacII SanDI
SfiI SgrAI SrfI SspI SwaI XhoI ZraI