Restriction map for pF1KB9838
 (Linear) (Six-base) MAPSORT of: KIBB9838.seq  Check: 194  from: 1  to: 1056



KIBB9838.orf

 With 182 enzymes: SgfI * 

                        August 18, 2011 15:44  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     720     904    1056 
   Size:        720     184     152

  Fragments arranged by size: 

                 720     184     152
 
AfeI AGC'GCT

Cuts at:      0     118     673    1056 
   Size:        118     555     383

  Fragments arranged by size: 

                 555     383     118
 
AleI CACnn'nnGTG

Cuts at:      0     638    1056 
   Size:        638     418
 
AlwNI CAG_nnn'CTG

Cuts at:      0     203    1056 
   Size:        203     853
 
ApaI G_GGCC'C

Cuts at:      0     301    1056 
   Size:        301     755
 
AvaI C'yCGr_G

Cuts at:      0     106     216     280     321     382     403     504     964 
   Size:        106     110      64      41      61      21     101     460

Cuts at:    964    1056 
   Size:         92

  Fragments arranged by size: 

                 460     110     106     101      92      64      61      41
                  21
 
BanI G'GyrC_C

Cuts at:      0      22     220     274     304     316     541     899    1056 
   Size:         22     198      54      30      12     225     358     157

  Fragments arranged by size: 

                 358     225     198     157      54      30      22      12
            
 
BanII G_rGCy'C

Cuts at:      0     301    1056 
   Size:        301     755
 
BbeI G_GCGC'C

Cuts at:      0      26     278     308     545     903    1056 
   Size:         26     252      30     237     358     153

  Fragments arranged by size: 

                 358     252     237     153      30      26
 
BglI GCCn_nnn'nGGC

Cuts at:      0     443     490     959    1056 
   Size:        443      47     469      97

  Fragments arranged by size: 

                 469     443      97      47
 
BlpI GC'TnA_GC

Cuts at:      0     163    1056 
   Size:        163     893
 
Bme1580I G_kGCm'C

Cuts at:      0     188     223     301     319    1039    1056 
   Size:        188      35      78      18     720      17

  Fragments arranged by size: 

                 720     188      78      35      18      17
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     693    1056 
   Size:        693     363
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     685     847     862     910    1056 
   Size:        358     327     162      15      48     146

  Fragments arranged by size: 

                 358     327     162     146      48      15
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     745     766    1056 
   Size:        745      21     290

  Fragments arranged by size: 

                 745     290      21
 
BsaAI yAC'GTr

Cuts at:      0     657    1056 
   Size:        657     399
 
BsaHI Gr'CG_yC

Cuts at:      0      23     157     275     305     400     542     900    1056 
   Size:         23     134     118      30      95     142     358     156

  Fragments arranged by size: 

                 358     156     142     134     118      95      30      23
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     331     361    1056 
   Size:        331      30     695

  Fragments arranged by size: 

                 695     331      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     168     180     682     760    1056 
   Size:        168      12     502      78     296

  Fragments arranged by size: 

                 502     296     168      78      12
 
BseYI C'CCAG_C

Cuts at:      0      34     196     211    1056 
   Size:         34     162      15     845

  Fragments arranged by size: 

                 845     162      34      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     844    1056 
   Size:        844     212
 
BsiEI CG_ry'CG

Cuts at:      0     476    1043    1056 
   Size:        476     567      13

  Fragments arranged by size: 

                 567     476      13
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     266    1056 
   Size:        266     790
 
Bsp1286I G_dGCh'C

Cuts at:      0     188     223     301     319    1039    1056 
   Size:        188      35      78      18     720      17

  Fragments arranged by size: 

                 720     188      78      35      18      17
 
BsrBI CCG'CTC

Cuts at:      0     162     293     359     529     915    1056 
   Size:        162     131      66     170     386     141

  Fragments arranged by size: 

                 386     170     162     141     131      66
 
BsrFI r'CCGG_y

Cuts at:      0     223     330     544     719     982    1015    1056 
   Size:        223     107     214     175     263      33      41

  Fragments arranged by size: 

                 263     223     214     175     107      41      33
 
BsrGI T'GTAC_A

Cuts at:      0     923    1056 
   Size:        923     133
 
BssHII G'CGCG_C

Cuts at:      0     206     446     458     560    1056 
   Size:        206     240      12     102     496

  Fragments arranged by size: 

                 496     240     206     102      12
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     173    1056 
   Size:        173     883
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     619    1056 
   Size:        619     437
 
BstYI r'GATC_y

Cuts at:      0     342    1056 
   Size:        342     714
 
BtgI C'CryG_G

Cuts at:      0       5     441     453     498     555     618     729     789 
   Size:          5     436      12      45      57      63     111      60

Cuts at:    789     991    1056 
   Size:        202      65

  Fragments arranged by size: 

                 436     202     111      65      63      60      57      45
                  12       5
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      31    1056 
   Size:         31    1025
 
DraIII CAC_nnn'GTG

Cuts at:      0     657    1056 
   Size:        657     399
 
EaeI y'GGCC_r

Cuts at:      0       2      47     435     473     546     905    1040    1056 
   Size:          2      45     388      38      73     359     135      16

  Fragments arranged by size: 

                 388     359     135      73      45      38      16       2
            
 
EagI C'GGCC_G

Cuts at:      0     473    1040    1056 
   Size:        473     567      16

  Fragments arranged by size: 

                 567     473      16
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     539    1056 
   Size:        539     517
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     685     720     847     862     904     910    1056 
   Size:        358     327      35     127      15      42       6     146

  Fragments arranged by size: 

                 358     327     146     127      42      35      15       6
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     925    1056 
   Size:        925     131
 
EcoO109I rG'GnC_Cy

Cuts at:      0     297     298     918    1056 
   Size:        297       1     620     138

  Fragments arranged by size: 

                 620     297     138       1
 
HaeII r_GCGC'y

Cuts at:      0      26     120     278     308     337     391     424     545 
   Size:         26      94     158      30      29      54      33     121

Cuts at:    545     675     903    1056 
   Size:        130     228     153

  Fragments arranged by size: 

                 228     158     153     130     121      94      54      33
                  30      29      26
 
HincII GTy'rAC

Cuts at:      0      63    1056 
   Size:         63     993
 
KasI G'GCGC_C

Cuts at:      0      22     274     304     541     899    1056 
   Size:         22     252      30     237     358     157

  Fragments arranged by size: 

                 358     252     237     157      30      22
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     704    1056 
   Size:        704     352
 
MscI TGG'CCA

Cuts at:      0      49     907    1056 
   Size:         49     858     149

  Fragments arranged by size: 

                 858     149      49
 
MslI CAynn'nnrTG

Cuts at:      0     617     638     703    1056 
   Size:        617      21      65     353

  Fragments arranged by size: 

                 617     353      65      21
 
MspA1I CmG'CkG

Cuts at:      0       7      45     434     500     690     731     791     831 
   Size:          7      38     389      66     190      41      60      40

Cuts at:    831     882     993    1056 
   Size:         51     111      63

  Fragments arranged by size: 

                 389     190     111      66      63      60      51      41
                  40      38       7
 
NaeI GCC'GGC

Cuts at:      0     332     546    1056 
   Size:        332     214     510

  Fragments arranged by size: 

                 510     332     214
 
NarI GG'CG_CC

Cuts at:      0      23     275     305     542     900    1056 
   Size:         23     252      30     237     358     156

  Fragments arranged by size: 

                 358     252     237     156      30      23
 
NcoI C'CATG_G

Cuts at:      0     618    1056 
   Size:        618     438
 
NgoMIV G'CCGG_C

Cuts at:      0     330     544    1056 
   Size:        330     214     512

  Fragments arranged by size: 

                 512     330     214
 
NotI GC'GGCC_GC

Cuts at:      0     473    1056 
   Size:        473     583
 
PmlI CAC'GTG

Cuts at:      0     657    1056 
   Size:        657     399
 
PpuMI rG'GwC_Cy

Cuts at:      0     918    1056 
   Size:        918     138
 
PspOMI G'GGCC_C

Cuts at:      0     297    1056 
   Size:        297     759
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     778     810    1056 
   Size:        778      32     246

  Fragments arranged by size: 

                 778     246      32
 
PvuII CAG'CTG

Cuts at:      0     690     882    1056 
   Size:        690     192     174

  Fragments arranged by size: 

                 690     192     174
 
SacII CC_GC'GG

Cuts at:      0       8     501     732     792     994    1056 
   Size:          8     493     231      60     202      62

  Fragments arranged by size: 

                 493     231     202      62      60       8
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     959    1056 
   Size:        959      97
 
SfoI GGC'GCC

Cuts at:      0      24     276     306     543     901    1056 
   Size:         24     252      30     237     358     155

  Fragments arranged by size: 

                 358     252     237     155      30      24
 
SgrAI Cr'CCGG_yG

Cuts at:      0     223    1056 
   Size:        223     833
 
SmaI CCC'GGG

Cuts at:      0     218     282     506     966    1056 
   Size:        218      64     224     460      90

  Fragments arranged by size: 

                 460     224     218      90      64
 
SmlI C'TyrA_G

Cuts at:      0     760     781    1056 
   Size:        760      21     275

  Fragments arranged by size: 

                 760     275      21
 
SrfI GCCC'GGGC

Cuts at:      0     218     506    1056 
   Size:        218     288     550

  Fragments arranged by size: 

                 550     288     218
 
StyI C'CwwG_G

Cuts at:      0     618    1031    1056 
   Size:        618     413      25

  Fragments arranged by size: 

                 618     413      25
 
TatI w'GTAC_w

Cuts at:      0     347     923    1056 
   Size:        347     576     133

  Fragments arranged by size: 

                 576     347     133
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      42     616    1056 
   Size:         42     574     440

  Fragments arranged by size: 

                 574     440      42
 
XmaI C'CCGG_G

Cuts at:      0     216     280     504     964    1056 
   Size:        216      64     224     460      92

  Fragments arranged by size: 

                 460     224     216      92      64
 

 Enzymes that do cut:

     AcuI     AfeI     AleI    AlwNI     ApaI     AvaI     BanI    BanII
     BbeI     BglI     BlpI Bme1580I     BplI     BpmI    BpuEI    BsaAI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsmBI Bsp1286I
    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstXI    BstYI     BtgI
    BtgZI   DraIII     EaeI     EagI     EciI  Eco57MI    EcoNI EcoO109I
    HaeII   HincII     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NotI     PmlI    PpuMI   PspOMI     PsrI
    PvuII    SacII     SfiI     SfoI    SgrAI     SmaI     SmlI     SrfI
     StyI     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII    BmgBI     BmrI     BmtI   Bpu10I     BsaI    BsaBI
    BsaWI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrDI
    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI     DrdI     EarI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI
     NdeI     NheI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI    PspXI     PstI
     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SgfI    SnaBI     SpeI     SphI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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