Restriction map for pF1KE2368
##################################
# NO CUT ENZYMES (   48 enzymes) #
##################################

AclI        AflII       AscI        AseI        AsnI        
AvrII       BbrPI       BclI        BfrI        BlnI        
BsiWI       BsmI        BspDI       BssHII      Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        EcoRV       
EcoT22I     FbaI        FseI        HindIII     HpaI        
MfeI        MunI        NotI        NruI        NsiI        
NspV        PacI        PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RsrII       SalI        
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Acc65I       4220  GGTACC               G^GTACC
AccI         3832  GTAGAC               GT^[AC][GT]AC
AgeI         1016  ACCGGT               A^CCGGT
AhdI         6028  GACCGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I      4220  GGTACC               G^GTACC
AspEI        6028  GACCGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         5929  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         3982  GGCGCC               GGCGC^C
BciVI        1359  GGATAC               GTATCC(6/5)
BsaAI        5845  TACGTG               [CT]AC^GT[GA]
BsaBI        2109  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        5126  CGGCCG               CG[GA][CT]^CG
BssSI        1333  CACGAG               CACGAG(-5/-1)
BstEII       4623  GGTGACC              G^GT[ACGT]ACC
BstPI        4623  GGTGACC              G^GT[ACGT]ACC
DraI         4249  TTTAAA               TTT^AAA
EagI         5126  CGGCCG               C^GGCCG
Eam1105I     6028  GACCGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        5126  CGGCCG               C^GGCCG
Eco52I       5126  CGGCCG               C^GGCCG
EcoO65I      4623  GGTGACC              G^GT[ACGT]ACC
EcoRI        2230  GAATTC               G^AATTC
KasI         3982  GGCGCC               G^GCGCC
KpnI         4220  GGTACC               GGTAC^C
MamI         2109  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI          551  ACGCGT               A^CGCGT
MseI         4250  TTAA                 T^TAA
NaeI         5128  GCCGGC               GCC^GGC
NarI         3982  GGCGCC               GG^CGCC
NdeI         3737  CATATG               CA^TATG
NgoMIV       5128  GCCGGC               G^CCGGC
NheI         2535  GCTAGC               G^CTAGC
PaeR7I        533  CTCGAG               C^TCGAG
PflFI        5929  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1016  ACCGGT               A^CCGGT
PshAI        4648  GACCAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI          171  CCTGCAGG             CCTGCA^GG
SfoI         3982  GGCGCC               GGC^GCC
SmaI         3115  CCCGGG               CCC^GGG
Sse8387I      171  CCTGCAGG             CCTGCA^GG
Tru9I        4250  TTAA                 T^TAA
Tth111I      5929  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          533  CTCGAG               C^TCGAG
XmaCI        3115  CCCGGG               C^CCGGG
XmaI         3115  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AatII        3835  GACGTC               GACGT^C
             4588  GACGTC              
Alw44I        414  GTGCAC               G^TGCAC
             3914  GTGCAC              
AocI          825  CCTAAGG              CC^T[ACGT]AGG
             1143  CCTCAGG             
ApaLI         414  GTGCAC               G^TGCAC
             3914  GTGCAC              
AviII         842  TGCGCA               TGC^GCA
             3578  TGCGCA              
BamHI        3120  GGATCC               G^GATCC
             4922  GGATCC              
BglI         1908  GCCAGGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2628  GCCTCGCTGGC         
BlpI         2814  GCTCAGC              GC^T[ACGT]AGC
             3439  GCTGAGC             
Bpu1102I     2814  GCTCAGC              GC^T[ACGT]AGC
             3439  GCTGAGC             
BsgI          547  CTGCAC               GTGCAG(16/14)
             2828  GTGCAG              
Bsp1407I     4334  TGTACA               T^GTACA
             5294  TGTACA              
BsrBI         365  GAGCGG               CCGCTC(-3/-3)
             3688  GAGCGG              
BsrFI        1016  ACCGGT               [GA]^CCGG[CT]
             5128  GCCGGC              
BsrGI        4334  TGTACA               T^GTACA
             5294  TGTACA              
Bsu36I        825  CCTAAGG              CC^T[ACGT]AGG
             1143  CCTCAGG             
CelII        2814  GCTCAGC              GC^T[ACGT]AGC
             3439  GCTGAGC             
Cfr10I       1016  ACCGGT               [GA]^CCGG[CT]
             5128  GCCGGC              
DraIII       2169  CACTATGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3595  CACATCGTG           
Eco81I        825  CCTAAGG              CC^T[ACGT]AGG
             1143  CCTCAGG             
FspI          842  TGCGCA               TGC^GCA
             3578  TGCGCA              
HincII       4195  GTCAAC               GT[CT]^[GA]AC
             5287  GTCAAC              
HindII       4195  GTCAAC               GT[CT]^[GA]AC
             5287  GTCAAC              
KspI           99  CCGCGG               CCGC^GG
             1575  CCGCGG              
SacII          99  CCGCGG               CCGC^GG
             1575  CCGCGG              
SexAI        1208  ACCTGGT              A^CC[AT]GGT
             2258  ACCTGGT             
SspBI        4334  TGTACA               T^GTACA
             5294  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  139 enzymes) #
#########################################

AccII       AccIII      AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
Aor51HI     ApaI        ApoI        Asp700I     AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvCI       BbvI        BcgI        BcnI        
BfaI        BglII       BmyI        BpmI        BpuAI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspEI       
BspHI       BspLU11I    BspMI       BsrDI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DrdI        DsaI        
EaeI        EarI        Eco47III    EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MroI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RcaI        
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SmlI        
SphI        StuI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmnI        
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