(Circular) (Six-base) MAPSORT of: pF1KE2368.seq Check: 5871 from: 1 to: 9158 pF1KE2368 9158 bp With 182 enzymes: SgfI * September 22, 2011 10:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4335 5858 4335 Size: 1523 7635 AatII G_ACGT'C Cuts at: 3947 4700 3947 Size: 753 8405 AccI GT'mk_AC Cuts at: 3941 6196 3941 Size: 2255 6903 Acc65I G'GTAC_C Cuts at: 4328 6174 4328 Size: 1846 7312 AclI AA'CG_TT Cuts at: 8998 8998 Size: 9158 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 835 1541 1745 2916 3632 4131 5412 5737 6983 Size: 706 204 1171 716 499 1281 325 1246 Cuts at: 6983 7415 8162 8430 835 Size: 432 747 268 1563 Fragments arranged by size: 1563 1281 1246 1171 747 716 706 499 432 325 268 204 AfeI AGC'GCT Cuts at: 965 1405 2185 3767 965 Size: 440 780 1582 6356 Fragments arranged by size: 6356 1582 780 440 AflIII A'CryG_T Cuts at: 659 1108 4439 5174 5399 6510 7614 8591 659 Size: 449 3331 735 225 1111 1104 977 1226 Fragments arranged by size: 3331 1226 1111 1104 977 735 449 225 AgeI A'CCGG_T Cuts at: 1124 7546 1124 Size: 6422 2736 AhdI GACnn_n'nnGTC Cuts at: 6141 6141 Size: 9158 AleI CACnn'nnGTG Cuts at: 4234 4234 Size: 9158 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 5866 5866 Size: 9158 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1836 1868 5722 5754 1836 Size: 32 3854 32 5240 Fragments arranged by size: 5240 3854 32 32 AlwNI CAG_nnn'CTG Cuts at: 1564 2034 2956 3386 3406 6463 8030 1564 Size: 470 922 430 20 3057 1567 2692 Fragments arranged by size: 3057 2692 1567 922 470 430 20 ApaI G_GGCC'C Cuts at: 786 834 2027 2094 3997 786 Size: 48 1193 67 1903 5947 Fragments arranged by size: 5947 1903 1193 67 48 ApaLI G'TGCA_C Cuts at: 522 4022 7928 522 Size: 3500 3906 1752 Fragments arranged by size: 3906 3500 1752 ApoI r'AATT_y Cuts at: 730 2338 5503 6162 8511 8767 730 Size: 1608 3165 659 2349 256 1121 Fragments arranged by size: 3165 2349 1608 1121 659 256 AseI AT'TA_AT Cuts at: 20 20 Size: 9158 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 9158 AvaI C'yCGr_G Cuts at: 128 247 641 3223 5318 6178 128 Size: 119 394 2582 2095 860 3108 Fragments arranged by size: 3108 2582 2095 860 394 119 BamHI G'GATC_C Cuts at: 3228 5030 6183 3228 Size: 1802 1153 6203 Fragments arranged by size: 6203 1802 1153 BanI G'GyrC_C Cuts at: 133 973 1063 1127 1794 3162 3217 4090 4328 Size: 840 90 64 667 1368 55 873 238 Cuts at: 4328 5846 6174 6588 6836 6871 133 Size: 1518 328 414 248 35 2420 Fragments arranged by size: 2420 1518 1368 873 840 667 414 328 248 238 90 64 55 35 BanII G_rGCy'C Cuts at: 332 344 786 834 2027 2094 3239 3554 3997 Size: 12 442 48 1193 67 1145 315 443 Cuts at: 3997 4044 4612 5338 5459 5777 6172 7202 332 Size: 47 568 726 121 318 395 1030 2288 Fragments arranged by size: 2288 1193 1145 1030 726 568 443 442 395 318 315 121 67 48 47 12 BbeI G_GCGC'C Cuts at: 4094 6592 6840 4094 Size: 2498 248 6412 Fragments arranged by size: 6412 2498 248 BbsI GAAGACnn'nnnn_ Cuts at: 823 873 1547 2364 2544 2853 3808 3843 823 Size: 50 674 817 180 309 955 35 6138 Fragments arranged by size: 6138 955 817 674 309 180 50 35 BbvCI CC'TCA_GC Cuts at: 889 1651 2095 2710 5254 889 Size: 762 444 615 2544 4793 Fragments arranged by size: 4793 2544 762 615 444 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 632 666 1162 1196 3412 3446 5905 5939 632 Size: 34 496 34 2216 34 2459 34 3851 Fragments arranged by size: 3851 2459 2216 496 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 1461 7056 7817 9158 1461 Size: 5595 761 1341 1461 Fragments arranged by size: 5595 1461 1341 761 BclI T'GATC_A Cuts at: 6678 6678 Size: 9158 BglI GCCn_nnn'nGGC Cuts at: 2022 2742 2022 Size: 720 8438 BglII A'GATC_T Cuts at: 303 1268 5492 5762 6102 6673 8710 303 Size: 965 4224 270 340 571 2037 751 Fragments arranged by size: 4224 2037 965 751 571 340 270 BlpI GC'TnA_GC Cuts at: 2923 3548 6270 2923 Size: 625 2722 5811 Fragments arranged by size: 5811 2722 625 Bme1580I G_kGCm'C Cuts at: 136 526 786 834 1799 1824 2027 2094 2622 Size: 390 260 48 965 25 203 67 528 Cuts at: 2622 3220 3251 3313 3997 4026 4921 6783 6876 Size: 598 31 62 684 29 895 1862 93 Cuts at: 6876 7932 136 Size: 1056 1362 Fragments arranged by size: 1862 1362 1056 965 895 684 598 528 390 260 203 93 67 62 48 31 29 25 BmgBI CAC'GTC Cuts at: 5231 5231 Size: 9158 BmrI ACTGGGnnnn_n' Cuts at: 1504 1829 1927 2632 5282 6370 6451 6786 8930 Size: 325 98 705 2650 1088 81 335 2144 Cuts at: 8930 1504 Size: 1732 Fragments arranged by size: 2650 2144 1732 1088 705 335 325 98 81 BmtI G_CTAG'C Cuts at: 2647 2647 Size: 9158 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5959 5988 7525 5959 Size: 29 1537 7592 Fragments arranged by size: 7592 1537 29 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 367 2335 2968 3408 3577 3880 4380 5440 5979 Size: 1968 633 440 169 303 500 1060 539 Cuts at: 5979 6052 367 Size: 73 3473 Fragments arranged by size: 3473 1968 1060 633 539 500 440 303 169 73 Bpu10I CC'TnA_GC Cuts at: 889 1651 2095 2479 2710 5254 5930 889 Size: 762 444 384 231 2544 676 4117 Fragments arranged by size: 4117 2544 762 676 444 384 231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1039 1414 3841 3856 6334 7213 7705 8003 1039 Size: 375 2427 15 2478 879 492 298 2194 Fragments arranged by size: 2478 2427 2194 879 492 375 298 15 BsaI GGTCTCn'nnnn_ Cuts at: 1378 1706 1914 2803 3061 3487 8862 1378 Size: 328 208 889 258 426 5375 1674 Fragments arranged by size: 5375 1674 889 426 328 258 208 BsaAI yAC'GTr Cuts at: 5955 6511 5955 Size: 556 8602 BsaBI GATnn'nnATC Cuts at: 6 2221 6672 8709 6 Size: 2215 4451 2037 455 Fragments arranged by size: 4451 2215 2037 455 BsaHI Gr'CG_yC Cuts at: 3944 4091 4697 5711 6589 6837 7539 9035 3944 Size: 147 606 1014 878 248 702 1496 4067 Fragments arranged by size: 4067 1496 1014 878 702 606 248 147 BsaWI w'CCGG_w Cuts at: 416 467 1113 1124 3352 3372 4273 6126 6347 Size: 51 646 11 2228 20 901 1853 221 Cuts at: 6347 6868 7546 7820 7967 8378 416 Size: 521 678 274 147 411 1196 Fragments arranged by size: 2228 1853 1196 901 678 646 521 411 274 221 147 51 20 11 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1836 1866 3307 3337 3496 3526 4939 4969 5722 Size: 30 1441 30 159 30 1413 30 753 Cuts at: 5722 5752 1836 Size: 30 5242 Fragments arranged by size: 5242 1441 1413 753 159 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 265 268 1372 1405 1554 1582 1951 2026 2083 Size: 3 1104 33 149 28 369 75 57 Cuts at: 2083 2152 2359 3310 3331 3334 3402 3808 4108 Size: 69 207 951 21 3 68 406 300 Cuts at: 4108 4585 5242 5458 5746 5991 6000 265 Size: 477 657 216 288 245 9 3423 Fragments arranged by size: 3423 1104 951 657 477 406 369 300 288 245 216 207 149 75 69 68 57 33 28 21 9 3 3 BseYI C'CCAG_C Cuts at: 269 554 1102 1226 2180 2493 3112 3341 4605 Size: 285 548 124 954 313 619 229 1264 Cuts at: 4605 5240 5337 6583 7341 7918 8414 269 Size: 635 97 1246 758 577 496 1013 Fragments arranged by size: 1264 1246 1013 954 758 635 619 577 548 496 313 285 229 124 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 640 2957 640 Size: 2317 6841 BsiEI CG_ry'CG Cuts at: 104 5237 6700 6746 7954 104 Size: 5133 1463 46 1208 1308 Fragments arranged by size: 5133 1463 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 332 526 1985 2082 2321 3554 3716 4026 4044 Size: 194 1459 97 239 1233 162 310 18 Cuts at: 4044 4928 5452 6172 6950 7140 7932 332 Size: 884 524 720 778 190 792 1558 Fragments arranged by size: 1558 1459 1233 884 792 778 720 524 310 239 194 190 162 97 18 BsmBI CGTCTCn'nnnn_ Cuts at: 2179 3145 3951 2179 Size: 966 806 7386 Fragments arranged by size: 7386 966 806 Bsp1286I G_dGCh'C Cuts at: 136 332 344 526 786 834 1799 1824 1985 Size: 196 12 182 260 48 965 25 161 Cuts at: 1985 2027 2082 2094 2321 2622 3220 3239 3251 Size: 42 55 12 227 301 598 19 12 Cuts at: 3251 3313 3554 3716 3997 4026 4044 4612 4921 Size: 62 241 162 281 29 18 568 309 Cuts at: 4921 4928 5338 5452 5459 5777 6172 6783 6876 Size: 7 410 114 7 318 395 611 93 Cuts at: 6876 6950 7140 7202 7932 136 Size: 74 190 62 730 1362 Fragments arranged by size: 1362 965 730 611 598 568 410 395 318 309 301 281 260 241 227 196 190 182 162 161 114 93 74 62 62 55 48 42 29 25 19 18 12 12 12 7 7 BspEI T'CCGG_A Cuts at: 467 1113 3372 6126 8378 467 Size: 646 2259 2754 2252 1247 Fragments arranged by size: 2754 2259 2252 1247 646 BspHI T'CATG_A Cuts at: 3743 4106 4562 5054 8334 3743 Size: 363 456 492 3280 4567 Fragments arranged by size: 4567 3280 492 456 363 BspMI ACCTGCnnnn'nnnn_ Cuts at: 140 287 1058 2346 3703 4335 4805 5630 5858 Size: 147 771 1288 1357 632 470 825 228 Cuts at: 5858 6208 6422 6724 7105 140 Size: 350 214 302 381 2193 Fragments arranged by size: 2193 1357 1288 825 771 632 470 381 350 302 228 214 147 BsrBI CCG'CTC Cuts at: 50 475 3798 7450 7504 8988 9153 50 Size: 425 3323 3652 54 1484 165 55 Fragments arranged by size: 3652 3323 1484 425 165 55 54 BsrDI GCAATG_nn' Cuts at: 753 1352 1628 1905 2411 3119 5316 5405 5649 Size: 599 276 277 506 708 2197 89 244 Cuts at: 5649 7070 753 Size: 1421 2841 Fragments arranged by size: 2841 2197 1421 708 599 506 277 276 244 89 BsrFI r'CCGG_y Cuts at: 1124 5236 7156 7337 7546 1124 Size: 4112 1920 181 209 2736 Fragments arranged by size: 4112 2736 1920 209 181 BsrGI T'GTAC_A Cuts at: 4442 5402 4442 Size: 960 8198 BssSI C'ACGA_G Cuts at: 1441 7429 7787 1441 Size: 5988 358 2812 Fragments arranged by size: 5988 2812 358 BstAPI GCAn_nnn'nTGC Cuts at: 1735 3091 3695 5210 5651 5853 6535 1735 Size: 1356 604 1515 441 202 682 4358 Fragments arranged by size: 4358 1515 1356 682 604 441 202 BstBI TT'CG_AA Cuts at: 7519 7519 Size: 9158 BstEII G'GTnAC_C Cuts at: 4731 4731 Size: 9158 BstXI CCAn_nnnn'nTGG Cuts at: 689 2124 2165 3258 3975 4743 5418 689 Size: 1435 41 1093 717 768 675 4429 Fragments arranged by size: 4429 1435 1093 768 717 675 41 BstYI r'GATC_y Cuts at: 1 303 1268 3228 3563 5030 5492 5720 5762 Size: 302 965 1960 335 1467 462 228 42 Cuts at: 5762 6102 6183 6650 6673 7008 7254 8266 8352 Size: 340 81 467 23 335 246 1012 86 Cuts at: 8352 8364 8710 1 Size: 12 346 449 Fragments arranged by size: 1960 1467 1012 965 467 462 449 346 340 335 335 302 246 228 86 81 42 23 12 Bsu36I CC'TnA_GG Cuts at: 934 1252 934 Size: 318 8840 BtgI C'CryG_G Cuts at: 107 207 682 986 1155 1337 1418 1644 1683 Size: 100 475 304 169 182 81 226 39 Cuts at: 1683 1869 4636 5342 5438 5627 5819 107 Size: 186 2767 706 96 189 192 3446 Fragments arranged by size: 3446 2767 706 475 304 226 192 189 186 182 169 100 96 81 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1362 3237 5931 7108 7289 7452 1362 Size: 1875 2694 1177 181 163 3068 Fragments arranged by size: 3068 2694 1875 1177 181 163 BtsI GCAGTG_nn' Cuts at: 1786 3463 3765 4006 6401 1786 Size: 1677 302 241 2395 4543 Fragments arranged by size: 4543 2395 1677 302 241 DraI TTT'AAA Cuts at: 4359 6157 4359 Size: 1798 7360 DraIII CAC_nnn'GTG Cuts at: 2282 3708 2282 Size: 1426 7732 DrdI GACnn_nn'nnGTC Cuts at: 1003 1254 1835 6864 7722 1003 Size: 251 581 5029 858 2439 Fragments arranged by size: 5029 2439 858 581 251 EaeI y'GGCC_r Cuts at: 1334 1857 2561 3260 3576 3965 4784 4808 5132 Size: 523 704 699 316 389 819 24 324 Cuts at: 5132 5234 5435 5816 6743 6917 7308 7335 8680 Size: 102 201 381 927 174 391 27 1345 Cuts at: 8680 1334 Size: 1812 Fragments arranged by size: 1812 1345 927 819 704 699 523 391 389 381 324 316 201 174 102 27 24 EagI C'GGCC_G Cuts at: 5234 6743 5234 Size: 1509 7649 EarI CTCTTCn'nnn_ Cuts at: 508 925 1207 2123 2308 2911 3281 4403 4972 Size: 417 282 916 185 603 370 1122 569 Cuts at: 4972 5080 5101 5221 5359 5801 5890 5912 7181 Size: 108 21 120 138 442 89 22 1269 Cuts at: 7181 7391 508 Size: 210 2275 Fragments arranged by size: 2275 1269 1122 916 603 569 442 417 370 282 210 185 138 120 108 89 22 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1195 1954 2527 4969 7366 7676 7822 8968 1195 Size: 759 573 2442 2397 310 146 1146 1385 Fragments arranged by size: 2442 2397 1385 1146 759 573 310 146 EcoICRI GAG'CTC Cuts at: 330 3552 4042 6170 330 Size: 3222 490 2128 3318 Fragments arranged by size: 3318 3222 2128 490 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 367 835 1541 1745 2335 2916 2968 3408 3577 Size: 468 706 204 590 581 52 440 169 Cuts at: 3577 3632 3880 4131 4380 5412 5440 5737 5979 Size: 55 248 251 249 1032 28 297 242 Cuts at: 5979 6052 6983 7415 8162 8430 367 Size: 73 931 432 747 268 1095 Fragments arranged by size: 1095 1032 931 747 706 590 581 468 440 432 297 268 251 249 248 242 204 169 73 55 52 28 EcoNI CCTnn'n_nnAGG Cuts at: 1704 3577 4342 5464 5841 1704 Size: 1873 765 1122 377 5021 Fragments arranged by size: 5021 1873 1122 765 377 EcoO109I rG'GnC_Cy Cuts at: 351 678 783 830 831 1307 1446 2024 2091 Size: 327 105 47 1 476 139 578 67 Cuts at: 2091 3012 3366 3383 3477 3591 3660 3994 5685 Size: 921 354 17 94 114 69 334 1691 Cuts at: 5685 5940 6297 351 Size: 255 357 3212 Fragments arranged by size: 3212 1691 921 578 476 357 354 334 327 255 139 114 105 94 69 67 47 17 1 EcoRI G'AATT_C Cuts at: 2338 6162 2338 Size: 3824 5334 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 429 3918 4707 4968 7177 429 Size: 3489 789 261 2209 2410 Fragments arranged by size: 3489 2410 2209 789 261 FspI TGC'GCA Cuts at: 952 3688 6939 952 Size: 2736 3251 3171 Fragments arranged by size: 3251 3171 2736 HaeII r_GCGC'y Cuts at: 967 1236 1407 2187 2314 3630 3769 4094 6592 Size: 269 171 780 127 1316 139 325 2498 Cuts at: 6592 6840 7862 8841 967 Size: 248 1022 979 1284 Fragments arranged by size: 2498 1316 1284 1022 979 780 325 269 248 171 139 127 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 624 656 1459 1491 1927 1959 2707 2739 2789 Size: 32 803 32 436 32 748 32 50 Cuts at: 2789 2821 3307 3339 3496 3528 5551 5583 6127 Size: 32 486 32 157 32 2023 32 544 Cuts at: 6127 6159 7000 7030 7032 7062 624 Size: 32 841 30 2 30 2720 Fragments arranged by size: 2720 2023 841 803 748 544 486 436 157 50 32 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 4305 5397 6197 4305 Size: 1092 800 7266 Fragments arranged by size: 7266 1092 800 KasI G'GCGC_C Cuts at: 4090 6588 6836 4090 Size: 2498 248 6412 Fragments arranged by size: 6412 2498 248 KpnI G_GTAC'C Cuts at: 4332 6178 4332 Size: 1846 7312 MluI A'CGCG_T Cuts at: 659 8591 659 Size: 7932 1226 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2340 2463 7829 8013 2340 Size: 123 5366 184 3485 Fragments arranged by size: 5366 3485 184 123 MscI TGG'CCA Cuts at: 1859 2563 3967 4786 4810 5134 5437 6919 1859 Size: 704 1404 819 24 324 303 1482 4098 Fragments arranged by size: 4098 1482 1404 819 704 324 303 24 MslI CAynn'nnrTG Cuts at: 1423 2278 2653 2830 3256 3742 4066 4159 4234 Size: 855 375 177 426 486 324 93 75 Cuts at: 4234 4561 4705 4741 5614 7147 7274 1423 Size: 327 144 36 873 1533 127 3307 Fragments arranged by size: 3307 1533 873 855 486 426 375 327 324 177 144 127 93 75 36 MspA1I CmG'CkG Cuts at: 209 273 1685 1759 2088 2211 2988 3069 3108 Size: 64 1412 74 329 123 777 81 39 Cuts at: 3108 3116 3547 3719 4387 4874 5906 6269 6583 Size: 8 431 172 668 487 1032 363 314 Cuts at: 6583 6943 7956 8201 209 Size: 360 1013 245 1166 Fragments arranged by size: 1412 1166 1032 1013 777 668 487 431 363 360 329 314 245 172 123 81 74 64 39 8 NaeI GCC'GGC Cuts at: 5238 7339 5238 Size: 2101 7057 NarI GG'CG_CC Cuts at: 4091 6589 6837 4091 Size: 2498 248 6412 Fragments arranged by size: 6412 2498 248 NcoI C'CATG_G Cuts at: 107 682 1418 1869 5627 5819 107 Size: 575 736 451 3758 192 3446 Fragments arranged by size: 3758 3446 736 575 451 192 NdeI CA'TA_TG Cuts at: 3846 3846 Size: 9158 NgoMIV G'CCGG_C Cuts at: 5236 7337 5236 Size: 2101 7057 NheI G'CTAG_C Cuts at: 2643 2643 Size: 9158 NspI r_CATG'y Cuts at: 513 1112 2682 4443 5178 5208 5403 5875 6211 Size: 599 1570 1761 735 30 195 472 336 Cuts at: 6211 6711 7618 513 Size: 500 907 2053 Fragments arranged by size: 2053 1761 1570 907 735 599 500 472 336 195 30 PasI CC'CwG_GG Cuts at: 1824 3099 3481 3664 3975 1824 Size: 1275 382 183 311 7007 Fragments arranged by size: 7007 1275 382 311 183 PciI A'CATG_T Cuts at: 1108 4439 5174 5399 7614 1108 Size: 3331 735 225 2215 2652 Fragments arranged by size: 3331 2652 2215 735 225 PflMI CCAn_nnn'nTGG Cuts at: 2570 3386 3708 3914 4874 5655 2570 Size: 816 322 206 960 781 6073 Fragments arranged by size: 6073 960 816 781 322 206 PfoI T'CCnGG_A Cuts at: 1409 1839 3379 3554 5417 1409 Size: 430 1540 175 1863 5150 Fragments arranged by size: 5150 1863 1540 430 175 PmeI GTTT'AAAC Cuts at: 6157 6157 Size: 9158 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1051 1083 1836 1868 5722 5754 8297 8329 1051 Size: 32 753 32 3854 32 2543 32 1880 Fragments arranged by size: 3854 2543 1880 753 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 351 678 1307 3383 3591 5685 351 Size: 327 629 2076 208 2094 3824 Fragments arranged by size: 3824 2094 2076 629 327 208 PshAI GACnn'nnGTC Cuts at: 4760 4760 Size: 9158 PspOMI G'GGCC_C Cuts at: 782 830 2023 2090 3993 782 Size: 48 1193 67 1903 5947 Fragments arranged by size: 5947 1903 1193 67 48 PspXI vC'TCGA_Gb Cuts at: 641 641 Size: 9158 PstI C_TGCA'G Cuts at: 284 917 2357 4346 6205 6890 284 Size: 633 1440 1989 1859 685 2552 Fragments arranged by size: 2552 1989 1859 1440 685 633 PvuI CG_AT'CG Cuts at: 104 104 Size: 9158 PvuII CAG'CTG Cuts at: 273 2988 3108 3116 3719 4387 4874 6583 6943 Size: 2715 120 8 603 668 487 1709 360 Cuts at: 6943 273 Size: 2488 Fragments arranged by size: 2715 2488 1709 668 603 487 360 120 8 RsrII CG'GwC_CG Cuts at: 7353 7353 Size: 9158 SacI G_AGCT'C Cuts at: 332 3554 4044 6172 332 Size: 3222 490 2128 3318 Fragments arranged by size: 3318 3222 2128 490 SacII CC_GC'GG Cuts at: 210 1686 210 Size: 1476 7682 SalI G'TCGA_C Cuts at: 6195 6195 Size: 9158 SanDI GG'GwC_CC Cuts at: 678 678 Size: 9158 SapI GCTCTTCn'nnn_ Cuts at: 508 925 4403 4972 5101 7181 7391 508 Size: 417 3478 569 129 2080 210 2275 Fragments arranged by size: 3478 2275 2080 569 417 210 129 SbfI CC_TGCA'GG Cuts at: 284 6205 284 Size: 5921 3237 ScaI AGT'ACT Cuts at: 3901 4585 4948 8675 3901 Size: 684 363 3727 4384 Fragments arranged by size: 4384 3727 684 363 SexAI A'CCwGG_T Cuts at: 1316 2366 1316 Size: 1050 8108 SfcI C'TryA_G Cuts at: 33 280 366 913 2353 4342 6087 6201 6886 Size: 247 86 547 1440 1989 1745 114 685 Cuts at: 6886 7879 8070 33 Size: 993 191 1121 Fragments arranged by size: 1989 1745 1440 1121 993 685 547 247 191 114 86 SfoI GGC'GCC Cuts at: 4092 6590 6838 4092 Size: 2498 248 6412 Fragments arranged by size: 6412 2498 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 9158 SmaI CCC'GGG Cuts at: 3225 6180 3225 Size: 2955 6203 SmlI C'TyrA_G Cuts at: 641 1054 1429 3820 3871 6313 7228 7720 7982 Size: 413 375 2391 51 2442 915 492 262 Cuts at: 7982 641 Size: 1817 Fragments arranged by size: 2442 2391 1817 915 492 413 375 262 51 SpeI A'CTAG_T Cuts at: 67 67 Size: 9158 SphI G_CATG'C Cuts at: 2682 5208 5875 6211 6711 2682 Size: 2526 667 336 500 5129 Fragments arranged by size: 5129 2526 667 500 336 StuI AGG'CCT Cuts at: 286 2729 4690 286 Size: 2443 1961 4754 Fragments arranged by size: 4754 2443 1961 StyI C'CwwG_G Cuts at: 107 682 1418 1487 1869 4292 5024 5627 5819 Size: 575 736 69 382 2423 732 603 192 Cuts at: 5819 5982 6292 6646 107 Size: 163 310 354 2619 Fragments arranged by size: 2619 2423 736 732 603 575 382 354 310 192 163 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 388 1402 3867 5529 6741 6874 7333 7542 388 Size: 1014 2465 1662 1212 133 459 209 2004 Fragments arranged by size: 2465 2004 1662 1212 1014 459 209 133 TatI w'GTAC_w Cuts at: 590 3899 4283 4442 4583 4946 5402 8673 590 Size: 3309 384 159 141 363 456 3271 1075 Fragments arranged by size: 3309 3271 1075 456 384 363 159 141 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4005 4037 8675 8707 4005 Size: 32 4638 32 4456 Fragments arranged by size: 4638 4456 32 32 Tth111I GACn'n_nGTC Cuts at: 6040 6955 6040 Size: 915 8243 XbaI T'CTAG_A Cuts at: 6189 6189 Size: 9158 XcmI CCAnnnn_n'nnnnTGG Cuts at: 984 1461 1867 3671 4602 5127 5685 6653 984 Size: 477 406 1804 931 525 558 968 3489 Fragments arranged by size: 3489 1804 968 931 558 525 477 406 XhoI C'TCGA_G Cuts at: 641 641 Size: 9158 XmaI C'CCGG_G Cuts at: 3223 6178 3223 Size: 2955 6203 XmnI GAAnn'nnTTC Cuts at: 2455 2905 3901 4882 4948 2455 Size: 450 996 981 66 6665 Fragments arranged by size: 6665 996 981 450 66 ZraI GAC'GTC Cuts at: 3945 4698 3945 Size: 753 8405 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AflII AscI AvrII BaeI BfrBI BsiWI BsmI BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NotI NruI NsiI PacI PmlI PsiI PsrI SfiI SgrAI SnaBI SrfI SspI SwaI