(Circular) (Six-base) MAPSORT of: pF1KE2321.seq Check: 674 from: 1 to: 7415 pF1KE2321 7415 bp With 182 enzymes: SgfI * January 27, 2011 13:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 798 3836 798 Size: 3038 4377 AatII G_ACGT'C Cuts at: 1166 1987 4178 1166 Size: 821 2191 4403 Fragments arranged by size: 4403 2191 821 AccI GT'mk_AC Cuts at: 4453 4453 Size: 7415 Acc65I G'GTAC_C Cuts at: 4431 4431 Size: 7415 AclI AA'CG_TT Cuts at: 7255 7255 Size: 7415 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 428 1178 1678 1819 1857 2878 4020 4159 5240 Size: 750 500 141 38 1021 1142 139 1081 Cuts at: 5240 5672 6419 6687 428 Size: 432 747 268 1156 Fragments arranged by size: 1156 1142 1081 1021 750 747 500 432 268 141 139 38 AflII C'TTAA_G Cuts at: 1075 1075 Size: 7415 AflIII A'CryG_T Cuts at: 119 3668 4767 5871 6848 119 Size: 3549 1099 1104 977 686 Fragments arranged by size: 3549 1104 1099 977 686 AgeI A'CCGG_T Cuts at: 5803 5803 Size: 7415 AhdI GACnn_n'nnGTC Cuts at: 846 846 Size: 7415 AleI CACnn'nnGTG Cuts at: 2188 3040 3221 3559 2188 Size: 852 181 338 6044 Fragments arranged by size: 6044 852 338 181 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2444 3473 3532 2444 Size: 1029 59 6327 Fragments arranged by size: 6327 1029 59 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 106 138 106 Size: 32 7383 AlwNI CAG_nnn'CTG Cuts at: 483 546 1865 1880 2010 2332 2909 3195 3356 Size: 63 1319 15 130 322 577 286 161 Cuts at: 3356 3495 4720 6287 483 Size: 139 1225 1567 1611 Fragments arranged by size: 1611 1567 1319 1225 577 322 286 161 139 130 63 15 ApaI G_GGCC'C Cuts at: 406 2708 406 Size: 2302 5113 ApaLI G'TGCA_C Cuts at: 2503 3685 6185 2503 Size: 1182 2500 3733 Fragments arranged by size: 3733 2500 1182 ApoI r'AATT_y Cuts at: 2581 4419 6768 7024 2581 Size: 1838 2349 256 2972 Fragments arranged by size: 2972 2349 1838 256 AseI AT'TA_AT Cuts at: 20 20 Size: 7415 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7415 AvaI C'yCGr_G Cuts at: 564 571 3719 4061 4435 564 Size: 7 3148 342 374 3544 Fragments arranged by size: 3544 3148 374 342 7 BamHI G'GATC_C Cuts at: 1376 2852 4440 1376 Size: 1476 1588 4351 Fragments arranged by size: 4351 1588 1476 BanI G'GyrC_C Cuts at: 256 786 1763 2275 2609 3871 3968 4431 4845 Size: 530 977 512 334 1262 97 463 414 Cuts at: 4845 5093 5128 256 Size: 248 35 2543 Fragments arranged by size: 2543 1262 977 530 512 463 414 334 248 97 35 BanII G_rGCy'C Cuts at: 406 1123 1325 1721 2708 2882 3139 3371 4137 Size: 717 202 396 987 174 257 232 766 Cuts at: 4137 4429 5459 406 Size: 292 1030 2362 Fragments arranged by size: 2362 1030 987 766 717 396 292 257 232 202 174 BbeI G_GCGC'C Cuts at: 260 4849 5097 260 Size: 4589 248 2578 Fragments arranged by size: 4589 2578 248 BbsI GAAGACnn'nnnn_ Cuts at: 882 1166 1987 2916 2937 3219 3954 4026 882 Size: 284 821 929 21 282 735 72 4271 Fragments arranged by size: 4271 929 821 735 284 282 72 21 BbvCI CC'TCA_GC Cuts at: 584 3505 584 Size: 2921 4494 BciVI GTATCCnnnnn_n' Cuts at: 3767 5313 6074 7415 3767 Size: 1546 761 1341 3767 Fragments arranged by size: 3767 1546 1341 761 BclI T'GATC_A Cuts at: 230 1209 4935 230 Size: 979 3726 2710 Fragments arranged by size: 3726 2710 979 BfrBI ATG'CAT Cuts at: 1131 1637 1131 Size: 506 6909 BglI GCCn_nnn'nGGC Cuts at: 698 2132 698 Size: 1434 5981 BglII A'GATC_T Cuts at: 2447 4348 4930 6967 2447 Size: 1901 582 2037 2895 Fragments arranged by size: 2895 2037 1901 582 BlpI GC'TnA_GC Cuts at: 745 4527 745 Size: 3782 3633 Bme1580I G_kGCm'C Cuts at: 406 789 1894 2165 2507 2708 3689 5040 5133 Size: 383 1105 271 342 201 981 1351 93 Cuts at: 5133 6189 406 Size: 1056 1632 Fragments arranged by size: 1632 1351 1105 1056 981 383 342 271 201 93 BmrI ACTGGGnnnn_n' Cuts at: 1047 2157 2848 4627 4708 5043 7187 1047 Size: 1110 691 1779 81 335 2144 1275 Fragments arranged by size: 2144 1779 1275 1110 691 335 81 BmtI G_CTAG'C Cuts at: 2326 2326 Size: 7415 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 633 709 5782 633 Size: 76 5073 2266 Fragments arranged by size: 5073 2266 76 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 370 448 1718 1873 1963 3038 3115 3295 3340 Size: 78 1270 155 90 1075 77 180 45 Cuts at: 3340 3592 370 Size: 252 4193 Fragments arranged by size: 4193 1270 1075 252 180 155 90 78 77 45 Bpu10I CC'TnA_GC Cuts at: 331 584 1715 3115 3505 331 Size: 253 1131 1400 390 4241 Fragments arranged by size: 4241 1400 1131 390 253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 709 1009 1294 4591 5470 5962 6260 709 Size: 300 285 3297 879 492 298 1864 Fragments arranged by size: 3297 1864 879 492 300 298 285 BsaI GGTCTCn'nnnn_ Cuts at: 370 424 4000 7119 370 Size: 54 3576 3119 666 Fragments arranged by size: 3576 3119 666 54 BsaAI yAC'GTr Cuts at: 384 4089 4768 384 Size: 3705 679 3031 Fragments arranged by size: 3705 3031 679 BsaBI GATnn'nnATC Cuts at: 6 2023 4929 6966 6 Size: 2017 2906 2037 455 Fragments arranged by size: 2906 2037 2017 455 BsaHI Gr'CG_yC Cuts at: 257 1163 1984 4175 4250 4846 5094 5796 7292 Size: 906 821 2191 75 596 248 702 1496 Cuts at: 7292 257 Size: 380 Fragments arranged by size: 2191 1496 906 821 702 596 380 248 75 BsaWI w'CCGG_w Cuts at: 2173 3770 4604 5125 5803 6077 6224 6635 2173 Size: 1597 834 521 678 274 147 411 2953 Fragments arranged by size: 2953 1597 834 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 2062 2092 2101 2131 2650 2665 2680 2695 2948 Size: 30 9 30 519 15 15 15 253 Cuts at: 2948 2978 3184 3214 4111 4141 2062 Size: 30 206 30 897 30 5336 Fragments arranged by size: 5336 897 519 253 206 30 30 30 30 30 15 15 15 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 2658 3337 3589 3648 3747 3824 2658 Size: 679 252 59 99 77 6249 Fragments arranged by size: 6249 679 252 99 77 59 BseYI C'CCAG_C Cuts at: 1553 1608 3617 3817 4223 4840 5598 6175 6671 Size: 55 2009 200 406 617 758 577 496 Cuts at: 6671 1553 Size: 2297 Fragments arranged by size: 2297 2009 758 617 577 496 406 200 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1401 1639 2644 3369 4111 1401 Size: 238 1005 725 742 4705 Fragments arranged by size: 4705 1005 742 725 238 BsiEI CG_ry'CG Cuts at: 104 315 530 885 4957 5003 6211 104 Size: 211 215 355 4072 46 1208 1308 Fragments arranged by size: 4072 1308 1208 355 215 211 46 BsiHKAI G_wGCw'C Cuts at: 1084 1325 1721 2507 2882 3371 3689 3816 4429 Size: 241 396 786 375 489 318 127 613 Cuts at: 4429 5207 5397 6189 1084 Size: 778 190 792 2310 Fragments arranged by size: 2310 792 786 778 613 489 396 375 318 241 190 127 BsiWI C'GTAC_G Cuts at: 4325 4325 Size: 7415 BsmI GAATG_Cn' Cuts at: 1359 3261 1359 Size: 1902 5513 BsmBI CGTCTCn'nnnn_ Cuts at: 169 2670 3175 169 Size: 2501 505 4409 Fragments arranged by size: 4409 2501 505 Bsp1286I G_dGCh'C Cuts at: 406 789 1084 1123 1325 1721 1894 2165 2507 Size: 383 295 39 202 396 173 271 342 Cuts at: 2507 2708 2882 3139 3371 3689 3816 4137 4429 Size: 201 174 257 232 318 127 321 292 Cuts at: 4429 5040 5133 5207 5397 5459 6189 406 Size: 611 93 74 190 62 730 1632 Fragments arranged by size: 1632 730 611 396 383 342 321 318 295 292 271 257 232 202 201 190 174 173 127 93 74 62 39 BspEI T'CCGG_A Cuts at: 2173 6635 2173 Size: 4462 2953 BspHI T'CATG_A Cuts at: 4217 6591 4217 Size: 2374 5041 BspMI ACCTGCnnnn'nnnn_ Cuts at: 798 1386 3836 4465 4679 4981 5362 798 Size: 588 2450 629 214 302 381 2851 Fragments arranged by size: 2851 2450 629 588 381 302 214 BsrBI CCG'CTC Cuts at: 50 210 1686 3270 3327 5707 5761 7245 7410 Size: 160 1476 1584 57 2380 54 1484 165 Cuts at: 7410 50 Size: 55 Fragments arranged by size: 2380 1584 1484 1476 165 160 57 55 54 BsrDI GCAATG_nn' Cuts at: 365 921 1713 2559 3006 3878 4358 5327 365 Size: 556 792 846 447 872 480 969 2453 Fragments arranged by size: 2453 969 872 846 792 556 480 447 BsrFI r'CCGG_y Cuts at: 314 525 529 2216 3597 3874 3971 4252 5413 Size: 211 4 1687 1381 277 97 281 1161 Cuts at: 5413 5594 5803 314 Size: 181 209 1926 Fragments arranged by size: 1926 1687 1381 1161 281 277 211 209 181 97 4 BsrGI T'GTAC_A Cuts at: 4046 4046 Size: 7415 BssSI C'ACGA_G Cuts at: 2780 3241 3250 5686 6044 2780 Size: 461 9 2436 358 4151 Fragments arranged by size: 4151 2436 461 358 9 BstAPI GCAn_nnn'nTGC Cuts at: 3495 4792 3495 Size: 1297 6118 BstBI TT'CG_AA Cuts at: 2234 5776 2234 Size: 3542 3873 BstEII G'GTnAC_C Cuts at: 2535 2535 Size: 7415 BstXI CCAn_nnnn'nTGG Cuts at: 426 504 639 2355 3561 3957 426 Size: 78 135 1716 1206 396 3884 Fragments arranged by size: 3884 1716 1206 396 135 78 BstYI r'GATC_y Cuts at: 1 507 1376 2447 2559 2852 4348 4440 4907 Size: 506 869 1071 112 293 1496 92 467 Cuts at: 4907 4930 5265 5511 6523 6609 6621 6967 1 Size: 23 335 246 1012 86 12 346 449 Fragments arranged by size: 1496 1071 1012 869 506 467 449 346 335 293 246 112 92 86 23 12 Bsu36I CC'TnA_GG Cuts at: 969 1486 969 Size: 517 6898 BtgI C'CryG_G Cuts at: 107 503 1421 1428 2483 3744 3950 4181 107 Size: 396 918 7 1055 1261 206 231 3341 Fragments arranged by size: 3341 1261 1055 918 396 231 206 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2714 5365 5546 5709 2714 Size: 2651 181 163 4420 Fragments arranged by size: 4420 2651 181 163 BtsI GCAGTG_nn' Cuts at: 557 4658 557 Size: 4101 3314 DraI TTT'AAA Cuts at: 4414 4414 Size: 7415 DraIII CAC_nnn'GTG Cuts at: 2817 2817 Size: 7415 DrdI GACnn_nn'nnGTC Cuts at: 1540 3621 3790 5121 5979 1540 Size: 2081 169 1331 858 2976 Fragments arranged by size: 2976 2081 1331 858 169 EaeI y'GGCC_r Cuts at: 312 527 1952 2345 2369 2744 3044 3599 3698 Size: 215 1425 393 24 375 300 555 99 Cuts at: 3698 5000 5174 5565 5592 6937 312 Size: 1302 174 391 27 1345 790 Fragments arranged by size: 1425 1345 1302 790 555 393 391 375 300 215 174 99 27 24 EagI C'GGCC_G Cuts at: 312 527 5000 312 Size: 215 4473 2727 Fragments arranged by size: 4473 2727 215 EarI CTCTTCn'nnn_ Cuts at: 172 1189 1262 1726 2632 2888 3010 3998 4367 Size: 1017 73 464 906 256 122 988 369 Cuts at: 4367 5438 5648 172 Size: 1071 210 1939 Fragments arranged by size: 1939 1071 1017 988 906 464 369 256 210 122 73 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1365 2070 3019 3361 4132 5623 5933 6079 7225 Size: 705 949 342 771 1491 310 146 1146 Cuts at: 7225 1365 Size: 1555 Fragments arranged by size: 1555 1491 1146 949 771 705 342 310 146 EcoICRI GAG'CTC Cuts at: 1323 1719 2880 3369 4427 1323 Size: 396 1161 489 1058 4311 Fragments arranged by size: 4311 1161 1058 489 396 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 370 428 448 1178 1678 1718 1819 1857 1873 Size: 58 20 730 500 40 101 38 16 Cuts at: 1873 1963 2878 3038 3115 3295 3340 3592 4020 Size: 90 915 160 77 180 45 252 428 Cuts at: 4020 4159 5240 5672 6419 6687 370 Size: 139 1081 432 747 268 1098 Fragments arranged by size: 1098 1081 915 747 730 500 432 428 268 252 180 160 139 101 90 77 58 45 40 38 20 16 EcoNI CCTnn'n_nnAGG Cuts at: 280 1693 1996 3508 280 Size: 1413 303 1512 4187 Fragments arranged by size: 4187 1512 1413 303 EcoO109I rG'GnC_Cy Cuts at: 186 402 403 452 487 560 568 650 663 Size: 216 1 49 35 73 8 82 13 Cuts at: 663 1605 1788 2285 2906 3246 4554 186 Size: 942 183 497 621 340 1308 3047 Fragments arranged by size: 3047 1308 942 621 497 340 216 183 82 73 49 35 13 8 1 EcoRI G'AATT_C Cuts at: 4419 4419 Size: 7415 EcoRV GAT'ATC Cuts at: 3921 3921 Size: 7415 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1551 1983 2940 4050 5434 1551 Size: 432 957 1110 1384 3532 Fragments arranged by size: 3532 1384 1110 957 432 FseI GG_CCGG'CC Cuts at: 318 318 Size: 7415 FspI TGC'GCA Cuts at: 5196 5196 Size: 7415 HaeII r_GCGC'y Cuts at: 260 347 1865 2011 4849 5097 6119 7098 260 Size: 87 1518 146 2838 248 1022 979 577 Fragments arranged by size: 2838 1518 1022 979 577 248 146 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 547 579 832 864 2062 2094 2948 2980 3568 Size: 32 253 32 1198 32 854 32 588 Cuts at: 3568 3600 5257 5287 5289 5319 547 Size: 32 1657 30 2 30 2643 Fragments arranged by size: 2643 1657 1198 854 588 253 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 309 2070 2967 3155 3294 4454 309 Size: 1761 897 188 139 1160 3270 Fragments arranged by size: 3270 1761 1160 897 188 139 HindIII A'AGCT_T Cuts at: 2423 3894 2423 Size: 1471 5944 KasI G'GCGC_C Cuts at: 256 4845 5093 256 Size: 4589 248 2578 Fragments arranged by size: 4589 2578 248 KpnI G_GTAC'C Cuts at: 4435 4435 Size: 7415 MluI A'CGCG_T Cuts at: 6848 6848 Size: 7415 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1727 1850 3843 3948 6086 6270 1727 Size: 123 1993 105 2138 184 2872 Fragments arranged by size: 2872 2138 1993 184 123 105 MscI TGG'CCA Cuts at: 1954 2371 2746 3700 5176 1954 Size: 417 375 954 1476 4193 Fragments arranged by size: 4193 1476 954 417 375 MslI CAynn'nnrTG Cuts at: 469 1114 2188 3040 3221 3559 5404 5531 469 Size: 645 1074 852 181 338 1845 127 2353 Fragments arranged by size: 2353 1845 1074 852 645 338 181 127 MspA1I CmG'CkG Cuts at: 2123 3011 3103 3447 3621 3909 4113 4526 4840 Size: 888 92 344 174 288 204 413 314 Cuts at: 4840 5200 6213 6458 2123 Size: 360 1013 245 3080 Fragments arranged by size: 3080 1013 888 413 360 344 314 288 245 204 174 92 NaeI GCC'GGC Cuts at: 316 531 3599 3973 4254 5596 316 Size: 215 3068 374 281 1342 2135 Fragments arranged by size: 3068 2135 1342 374 281 215 NarI GG'CG_CC Cuts at: 257 4846 5094 257 Size: 4589 248 2578 Fragments arranged by size: 4589 2578 248 NcoI C'CATG_G Cuts at: 107 2483 3950 4181 107 Size: 2376 1467 231 3341 Fragments arranged by size: 3341 2376 1467 231 NgoMIV G'CCGG_C Cuts at: 314 529 3597 3971 4252 5594 314 Size: 215 3068 374 281 1342 2135 Fragments arranged by size: 3068 2135 1342 374 281 215 NheI G'CTAG_C Cuts at: 2322 2322 Size: 7415 NsiI A_TGCA'T Cuts at: 1133 1639 1133 Size: 506 6909 NspI r_CATG'y Cuts at: 123 592 1147 1833 2266 3672 4468 4968 5875 Size: 469 555 686 433 1406 796 500 907 Cuts at: 5875 123 Size: 1663 Fragments arranged by size: 1663 1406 907 796 686 555 500 469 433 PasI CC'CwG_GG Cuts at: 483 1599 483 Size: 1116 6299 PciI A'CATG_T Cuts at: 119 3668 5871 119 Size: 3549 2203 1663 Fragments arranged by size: 3549 2203 1663 PflMI CCAn_nnn'nTGG Cuts at: 2010 2010 Size: 7415 PfoI T'CCnGG_A Cuts at: 1940 2828 1940 Size: 888 6527 PmeI GTTT'AAAC Cuts at: 4414 4414 Size: 7415 PmlI CAC'GTG Cuts at: 384 384 Size: 7415 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6554 6586 6554 Size: 32 7383 PpuMI rG'GwC_Cy Cuts at: 452 487 568 663 1605 1788 2285 2906 3246 Size: 35 81 95 942 183 497 621 340 Cuts at: 3246 452 Size: 4621 Fragments arranged by size: 4621 942 621 497 340 183 95 81 35 PspOMI G'GGCC_C Cuts at: 402 2704 402 Size: 2302 5113 PstI C_TGCA'G Cuts at: 1397 3200 3446 3452 3635 4016 4106 4462 5147 Size: 1803 246 6 183 381 90 356 685 Cuts at: 5147 1397 Size: 3665 Fragments arranged by size: 3665 1803 685 381 356 246 183 90 6 PvuI CG_AT'CG Cuts at: 104 104 Size: 7415 PvuII CAG'CTG Cuts at: 3011 3103 3447 3621 4113 4840 5200 3011 Size: 92 344 174 492 727 360 5226 Fragments arranged by size: 5226 727 492 360 344 174 92 RsrII CG'GwC_CG Cuts at: 5610 5610 Size: 7415 SacI G_AGCT'C Cuts at: 1325 1721 2882 3371 4429 1325 Size: 396 1161 489 1058 4311 Fragments arranged by size: 4311 1161 1058 489 396 SalI G'TCGA_C Cuts at: 4452 4452 Size: 7415 SanDI GG'GwC_CC Cuts at: 452 487 568 1605 2285 452 Size: 35 81 1037 680 5582 Fragments arranged by size: 5582 1037 680 81 35 SapI GCTCTTCn'nnn_ Cuts at: 1189 1726 2632 5438 5648 1189 Size: 537 906 2806 210 2956 Fragments arranged by size: 2956 2806 906 537 210 SbfI CC_TGCA'GG Cuts at: 4016 4106 4462 4016 Size: 90 356 6969 Fragments arranged by size: 6969 356 90 ScaI AGT'ACT Cuts at: 3409 6932 3409 Size: 3523 3892 SfcI C'TryA_G Cuts at: 33 1393 2037 3196 3442 3448 3631 3996 4012 Size: 1360 644 1159 246 6 183 365 16 Cuts at: 4012 4102 4458 5143 6136 6327 33 Size: 90 356 685 993 191 1121 Fragments arranged by size: 1360 1159 1121 993 685 644 365 356 246 191 183 90 16 6 SfoI GGC'GCC Cuts at: 258 4847 5095 258 Size: 4589 248 2578 Fragments arranged by size: 4589 2578 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7415 SgrAI Cr'CCGG_yG Cuts at: 4252 4252 Size: 7415 SmaI CCC'GGG Cuts at: 573 4063 4437 573 Size: 3490 374 3551 Fragments arranged by size: 3551 3490 374 SmlI C'TyrA_G Cuts at: 724 1024 1075 1309 3719 4570 5485 5977 6239 Size: 300 51 234 2410 851 915 492 262 Cuts at: 6239 724 Size: 1900 Fragments arranged by size: 2410 1900 915 851 492 300 262 234 51 SpeI A'CTAG_T Cuts at: 67 67 Size: 7415 SphI G_CATG'C Cuts at: 592 1147 4468 4968 592 Size: 555 3321 500 3039 Fragments arranged by size: 3321 3039 555 500 StuI AGG'CCT Cuts at: 1155 3400 1155 Size: 2245 5170 StyI C'CwwG_G Cuts at: 107 320 696 794 2471 2483 3950 4181 4549 Size: 213 376 98 1677 12 1467 231 368 Cuts at: 4549 4903 107 Size: 354 2619 Fragments arranged by size: 2619 1677 1467 376 368 354 231 213 98 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 899 2139 3140 3211 3831 4998 5131 5590 5799 Size: 1240 1001 71 620 1167 133 459 209 Cuts at: 5799 899 Size: 2515 Fragments arranged by size: 2515 1240 1167 1001 620 459 209 133 71 TatI w'GTAC_w Cuts at: 1106 3407 4046 6930 1106 Size: 2301 639 2884 1591 Fragments arranged by size: 2884 2301 1591 639 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 185 217 809 841 2801 2833 3806 3838 6932 Size: 32 592 32 1960 32 973 32 3094 Cuts at: 6932 6964 185 Size: 32 636 Fragments arranged by size: 3094 1960 973 636 592 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 886 5212 886 Size: 4326 3089 XbaI T'CTAG_A Cuts at: 4446 4446 Size: 7415 XcmI CCAnnnn_n'nnnnTGG Cuts at: 501 1251 4910 501 Size: 750 3659 3006 Fragments arranged by size: 3659 3006 750 XhoI C'TCGA_G Cuts at: 3719 3719 Size: 7415 XmaI C'CCGG_G Cuts at: 571 4061 4435 571 Size: 3490 374 3551 Fragments arranged by size: 3551 3490 374 XmnI GAAnn'nnTTC Cuts at: 2923 2923 Size: 7415 ZraI GAC'GTC Cuts at: 1164 1985 4176 1164 Size: 821 2191 4403 Fragments arranged by size: 4403 2191 821 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BcgI BmgBI BssHII BstZ17I ClaI CspCI FspAI HpaI MfeI NdeI NotI NruI PacI PshAI PsiI PspXI PsrI SacII SexAI SfiI SnaBI SrfI SspI SwaI